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常用生化试剂作用:
1、蛋白酶K:能水解消化蛋白质,特别是与DNA结合的组蛋白,在尿素和SDS中稳定。一般工作浓度是50—100μg/ml,推荐反应缓冲液:50mM Tris-HCl (pH7.5),10mM CaCl2。
2、SDS:十二烷基酸钠,溶解细胞膜上的脂类与蛋白质,因而溶解膜蛋白而破坏细胞膜,并解离细胞中的核蛋白,SDS 还能与蛋白质结合而沉淀。
3、IPTG:异丙基-β-D-硫代半乳糖苷, 常用于蓝白斑筛选及IPTG诱导的细菌内的蛋白表达等。IPTG和乳糖的结构相似,所以它和乳糖一样,可以与乳糖操纵元的阻遏蛋白结合,使阻遏蛋白的空间构像变化,四聚体解聚成单体,失去与操纵子特异性结合的能力,从而解除了阻遏蛋白的作用,使其后的基因得以转录合成利用乳糖的酶类。与乳糖不同的是,IPTG不被 β-半乳糖苷酶水解。常与X-GAL一起用于蓝白斑筛选。作用*的诱导剂,不被细菌代谢而十分稳定。
4、X-gal:5-溴-4-氯-3-吲哚-β-D-半乳糖苷,是IPTG的显色试剂,在IPTG的催化下显蓝色。常跟IPTG用与蓝白斑筛选。
5、G418:一种抗生素,对几乎所有的细胞都有毒性,是稳定转染常用的选择试剂。
6、DMSO:二甲基亚砜。实验室用于作液体层析溶剂,同用作测试物质紫外消光值上时用作参照物。能溶于所有烷烃和烯烃。
7、曲拉通X-100:TritonX-100,用的细胞裂解液成分之一,在保护蛋白活性方面有一定作用, 能力介于NP40和SDS之间,偏向于NP40。
8、NP-40:很温和的去垢剂,1%浓度的基本可以破坏掉胞膜,对核膜破坏的作用弱,结合特定的buffer可以获得胞浆蛋白。
9、BSA:牛血清白蛋白,主要起维持渗透压作用、PH缓冲作用、载体作用和营养作用。在动物细胞无血清培养中,添加白蛋白可起到生理和机械保护作用和载体作用。常用于蛋白定量中的内参和抗体稀释液。
10、甲酰胺: 甲酰胺被用作凝胶电泳中RNA的稳定剂,也用于稳定毛细管电泳中的变性单股DNA。
2017年产品报价如下:
货号 | 品名 | 规格 | 价格 | 品牌 |
M011-0 | mouse GPVI | 0.1 mg | 6375 | Emfret |
M011-1 | mouse GPVI | 1.5 ml; 300 tests | 7905 | Emfret |
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 0.5 mg | 7565 | Emfret |
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 1.5 ml; 300 tests | 7905 | Emfret |
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 1.5 ml; 300 tests | 8245 | Emfret |
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 1.5 ml; 300 tests | 8245 | Emfret |
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 0.5 mg | 7565 | Emfret |
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 1.5 ml; 300 tests | 7905 | Emfret |
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 0.5 mg | 7565 | Emfret |
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | 1.5 ml; 300 tests | 8245 | Emfret |
M024-0 | Integrin alphaIIb (GPIIb, CD41) | 6 mg | 34833 | Emfret |
M030-0 | Integrin beta3 chain (GPIIIa, CD61)mouse and human | 0.5 mg | 7565 | Emfret |
M031-0 | Integrin beta3 chain (GPIIIa, CD61)mouse and human | 0.5 mg | 7565 | Emfret |
M031-1 | Integrin beta3 chain (GPIIIa, CD61)mouse and human | 1.5 ml; 300 tests | 7905 | Emfret |
M040-0 | GPIbalpha (CD42b) | 0.5 mg | 7565 | Emfret |
M040-1 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 7905 | Emfret |
M040-2 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 8245 | Emfret |
M040-3 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 8245 | Emfret |
M041-0 | GPIbalpha (CD42b) | 0.5 mg | 7565 | Emfret |
M041-1 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 7905 | Emfret |
M042-0 | GPIbalpha (CD42b) | 0.5 mg | 7565 | Emfret |
M042-1 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 7905 | Emfret |
M043-0 | GPIbalpha (CD42b) | 0.5 mg | 7565 | Emfret |
M043-1 | GPIbalpha (CD42b) | 1.5 ml; 300 tests | 7905 | Emfret |
M050-0 | GPIbbeta (CD42c)mouse and human | 0.5 mg | 7565 | Emfret |
M050-1 | GPIbbeta (CD42c)mouse and human | 1.5 ml; 300 tests | 7905 | Emfret |
M051-0 | GPIX (CD42a) | 0.5 mg | 7565 | Emfret |
M051-1 | GPIX (CD42a) | 1.5 ml; 300 tests | 7905 | Emfret |
M060-1 | GPV (CD42d) | 1.5 ml; 300 tests | 7905 | Emfret |
M061-1 | GPV (CD42d) | 1.5 ml; 300 tests | 7905 | Emfret |
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | 0.5 mg | 7565 | Emfret |
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | 1.5 ml; 300 tests | 7905 | Emfret |
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | 0.5 mg | 7565 | Emfret |
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | 1.5 ml; 300 tests | 7905 | Emfret |
M080-0 | Integrin alpha5 chain (CD49e)mouse and human | 0.5 mg | 7565 | Emfret |
M080-1 | Integrin alpha5 chain (CD49e)mouse and human | 1.5 ml; 300 tests | 7905 | Emfret |
M110-0 | CD9 | 0.5 mg | 7565 | Emfret |
M110-1 | CD9 | 1.5 ml; 300 tests | 7905 | Emfret |
M120-0 | CD31 (PECAM-1) | 0.5 mg | 7565 | Emfret |
M130-0 | CD62P (P-selectin) | 0.5 mg | 7565 | Emfret |
M130-1 | CD63P (P-selectin) | 1.5 ml; 300 tests | 7905 | Emfret |
M130-2 | CD64P (P-selectin) | 1.5 ml; 300 tests | 8245 | Emfret |
P150-1 | von Willebrand Factor (vWF) | 1.5 ml; 300 tests | 7905 | Emfret |
P140-1 | fibrinogen | 1.5 ml; 300 tests | 7905 | Emfret |
P180-1 | negative control | 1.0 ml; 200 tests | 4063 | Emfret |
P190-1 | negative control | 1.0 ml; 200 tests | 4063 | Emfret |
P190-2 | negative control | 1.0 ml; 200 tests | 4063 | Emfret |
P190-3 | negative control | 1.0 ml; 200 tests | 4063 | Emfret |
R300 | Antibodies for Mouse Plaet Depletion | 0.5 mg | 7565 | Emfret |
C301 | Antibodies for Mouse Plaet Depletion | 0.5 mg | 1785 | Emfret |
D200 | Two-Color Analysis of Mouse Plaet Activation | 1 ml; 200 tests | 8313 | Emfret |
X488 | In Vivo Mouse Plaet Labeling | 0.1 mg | 8483 | Emfret |
X649 | In Vivo Mouse Plaet Labeling | 0.1 mg | 8483 | Emfret |