NGAL (human) ELISA kit
肾损伤标志物 NGAL 检测试剂盒
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NGAL (human) ELISA kit
肾损伤标志物 NGAL 检测试剂盒
急性肾损伤(Acute Kidney Injury,AKI)是一组临床综合征,以急性肾功能(Acute Renal Failure,ARF)减退为特征。针对 AKI 的诊断及研究,已证实存在于血液或尿液中的多种蛋白可成为肾损伤、肾疾病或肾毒性诊断及愈后的标志物。
其中,中性粒细胞明胶酶相关脂质运载蛋白(NGAL,lipocalin-2,siderocalin)是一种 25 kDa 的小蛋白,在全身上皮组织中表达。其可作为肾损伤标志物,当肾小管损伤后,NGAL 可从患者的尿液和血液中检出。
NGAL(人)ELISA 试剂盒,可在 450 nm 处高灵敏度检测人样本中 NGAL,低至 4 pg/mL。
◆特点
● 96 孔板检测,只需 4 小时即可快速检测最多 40 个样品及其平行组
● 高灵敏检测,可检测低至 4 pg/mL 的 NGAL
● 试剂盒包含即用型预涂板和独立标准溶液,可节省时间并减少操作失误
● 全定量分析,有别于半定量分析的免疫印迹法
◆NGAL(人)ELISA试剂盒(KIT 036)- 标准曲线
◆产品详情
NGAL别称 |
中性粒细胞明胶酶相关脂质运载蛋白、脂质运载蛋白2、铁运载蛋白、癌基因产物24p3 |
灵敏度 |
4 pg/mL(范围10-1000 pg/mL) |
检测时间 |
小于4小时 |
应用 |
ELISA、比色检测 |
应用说明 |
用于定量检测人尿液、血浆、血清、组织提取物和培养上清液中的NGAL |
波长 |
450 nm |
物种反应性 |
人 |
运输条件 |
非冷冻蓝冰 |
长期储存 |
+4°C |
组分 |
1. 12 × 8 孔微孔板条,每条均涂有抗 NGAL抗体(共96孔)+板条框 2. 样品稀释液 3. NGAL标准溶液1-8:0,10,25,50,100,250,500,1000 pg/mL 4. 25X浓缩洗涤液 5. 生物素化 NGAL 抗体 6. HRP-链霉亲和素偶联物 7. TMB底物 8. 终止液 |
技术信息/产品说明 |
点击此处查看操作手册 |
UniProt ID |
P80188 |
监管状态 |
RUO – 仅供研究用 |
制造商 |
BioPorto Diagnostics |
◆产品列表
产品编号 |
产品名称 |
包装 |
BPD-KIT-036 |
NGAL (human) ELISA kit |
96 wells |
◆其他物种的 NGAL 试剂盒
除NGAL (人) ELISA试剂盒外,另可提供小鼠、大鼠、猪、狗、猴的 NGAL ELISA 试剂盒。
产品编号 |
产品名称 |
包装 |
BPD-KIT-042 |
NGAL (mouse) ELISA kit |
96 wells |
BPD-KIT-043 |
NGAL (dog) ELISA kit |
96 wells |
BPD-KIT-044 |
NGAL (pig) ELISA kit |
96 wells |
BPD-KIT-045 |
NGAL (monkey) ELISA kit |
96 wells |
BPD-KIT-046 |
NGAL (rat) ELISA kit |
96 wells |
参考文献
1. |
The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males: T. Tominaga, et al.; Antioxidants (Basel) 10, 866 (2021), Application(s): NGAL quantification on human urine samples, 摘要; 全文 |
2. |
Prognostic value of urinary calprotectin, NGAL and KIM-1 in chronic kidney disease: F.S. Seibert, et al.; Kidney Blood Press. Res. 43, 1255 (2018), Application(s): ELISA using human urine, 摘要; |
3. |
Acute kidney injury prediction in cardiac surgery patients by a urinary peptide pattern: a case-control validation study: J. Metzger, et al.; Crit. Care 20, 157 (2016), 摘要; 全文 |
4. |
An AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions: J. Kanda, et al.; Clin. Exp. Nephrol. 19, 99 (2015), 摘要; |
5. |
Urinary neutrophil gelatinase-associated lipocalin predicts kidney outcome and death in patients with cirrhosis and bacterial infections: R. Barreto, et al.; J. Hepatol. 61, 35 (2014), Application(s): ELISA using human urine, 摘要; |
6. |
A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine: R.L. Kift, et al.; Ann. Clin. Biochem. 50, 236 (2013), 摘要; |
7. |
Albumin oxidation leads to neutrophil activation in vitro and inaccurate measurement of serum albumin in patients with diabetic nephropathy: R. Michelis, et al.; Free Radic. Biol. Med. 60, 49 (2013), 摘要; |
8. |
Association between plasma neutrophil gelatinase associated lipocalin level and obstructive sleep apnea or nocturnal intermittent hypoxia: K. Murase, et al.; PLoS One 8, e54184 (2013), 摘要; |
9. |
BK virus-associated nephropathy: neutrophil gelatinase-associated lipocalin as a new diagnostic tool?: S. Rau, et al.; Clin. Transplant. 27, E184 (2013), 摘要; |
10. |
Can neutrophil gelatinase-associated lipocalin help depict early contrast material-induced nephropathy?: A. Lacquaniti, et al.; Radiology 267, 86 (2013), 摘要; |
11. |
Circulating levels of neutrophil gelatinase-associated lipocalin (NGAL) correlate with the presence and severity of preeclampsia: S.M. Kim, et al.; Reprod. Sci. 20, 1083 (2013), 摘要; |
12. |
Day-to-day variation of urinary NGAL and rational for creatinine correction: J. Helmersson-Karlqvist, et al.; Clin. Biochem. 46, 70 (2013), 摘要; |
13. |
Differential expression of cancer-associated proteins in breastmilk: W. Qin, et al.; Breastfeed Med. 8, 120 (2013), 摘要; |
14. |
Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury: K. Kashani, et al.; Crit. Care 17, R25 (2013), 摘要; |
15. |
Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study: M. Di Nardo, et al.; Blood Purif. 35, 172 (2013), 摘要; |
16. |
Prognostic value of serum neutrophil gelatinase-associated lipocalin in metastatic and nonmetastatic colorectal cancer: J. Martí, et al.; World J. Surg. 37, 1103 (2013), 摘要; |
17. |
Prophylactic perioperative sodium bicarbonate to prevent acute kidney injury following open heart surgery: a multicenter double-blinded randomized controlled trial: M. Haase, et al.; PLoS Med. 10, e1001426 (2013), 摘要; |
18. |
Serum neutrophil gelatinase-associated lipocalin as a predictor of the development of bronchopulmonary dysplasia in preterm infants: H. Inoue, et al.; Early Hum. Dev. 89, 425 (2013), 摘要; |
19. |
The clinical utility of plasma neutrophil gelatinase-associated lipocalin in acute kidney injury: J.W. Pickering, et al.; Blood Purif. 35, 295 (2013), 摘要; |
20. |
The role of urinary liver-type fatty acid-binding protein in critically ill patients: E. Cho, et al.; J. Korean Med. Sci. 28, 100 (2013), 摘要; |
21. |
Urine Biomarkers Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule-1 (KIM-1) Have Different Patterns in Heart Failure Exacerbation: M. Park, et al.; Biomark. Insights 8, 15 (2013), 摘要; |
22. |
Albuminuria increases cystatin C excretion: implications for urinary biomarkers: M. Nejat, et al.; Nephrol. Dial. Transplant. 27 Suppl 3, iii96 (2012), 摘要; |
23. |
Are serum and urine neutrophil gelatinase-associated lipocalin predictive of renal graft function in short term?: N. Rahimzadeh, et al.; Pediatr. Transplant. 16, 796 (2012), 摘要; |
24. |
Association between systemic neutrophil gelatinase-associated lipocalin and anemia, relative hypochromia, and inflammation in chronic systolic heart failure: K. Shrestha, et al.; Congest. Heart Fail. 18, 239 (2012), 摘要; |
25. |
Delayed graft function (DGF) after living donor kidney transplantation: a study of possible explanatory factors: J. Salamzadeh, et al.; Ann. Transplant. 17, 69 (2012), 摘要; |
26. |
Evaluation of the impact of shock wave lithotripsy on kidneys using a new marker: how do neutrophil gelatinese-associated lypocalin values change after shock wave lithotripsy?: F. Zekey, et al.; Urology 80, 267 (2012), 摘要; |
27. |
Failure of remote ischemic preconditioning to reduce the risk of postoperative acute kidney injury in children undergoing operation for complex congenital heart disease: a randomized single-center study: K.R. Pedersen, et al.; J. Thorac. Cardiovasc. Surg. 143, 576 (2012), 摘要; |
28. |
Failure of remote ischemic preconditioning to reduce the risk of postoperative acute kidney injury in children undergoing operation for complex congenital heart disease: a randomized single-center study: K.R. Pedersen, et al.; J. Thorac. Cardiovasc. Surg. 143, 576 (2012), 摘要; |
29. |
Mild elevation of urinary biomarkers in prerenal acute kidney injury: K. Doi, et al.; Kidney Int. 82, 1114 (2012), 摘要; |
30. |
Neutrophil gelatinase-associated lipocalin as an early biomarker of acute kidney injury in liver transplantation: T.D. Jeong, et al.; Clin. Transplant. 26, 775 (2012), 摘要; |
31. |
Renal function and solitary kidney disease: Wilms tumour survivors versus patients with unilateral renal agenesis: J. Stefanowicz, et al.; Kidney Blood Press. Res. 35, 174 (2012), 摘要; |
32. |
Serum levels of gelatinase associated lipocalin as indicator of the inflammatory status in coronary artery disease: N. Kafkas, et al.; Int. J. Inflam. 2012, 189797 (2012), 摘要; |
33. |
Spironolactone diminishes urinary albumin excretion in patients with type 1 diabetes and microalbuminuria: a randomized placebo-controlled crossover study: S.E. Nielsen, et al.; Diabet. Med. 29, e184 (2012), 摘要; |
34. |
The effect of RAAS blockade on markers of renal tubular damage in diabetic nephropathy: u-NGAL, u-KIM1 and u-LFABP: S.E. Nielsen, et al.; Scand J. Clin. Lab Invest. 72, 137 (2012), 摘要; |
35. |
Urinary markers of kidney injury and kidney function decline in HIV-infected women: M.G. Shlipak, et al.; J. Acquir. Immune Defic. Syndr. 61, 565 (2012), 摘要; |
36. |
Urinary neutrophil gelatinase-associated lipocalin accurately detects acute allograft rejection among other causes of acute kidney injury in renal allograft recipients: N. Heyne, et al.; Transplantation 93, 1252 (2012), 摘要; |
37. |
Urinary NT-proBNP, NGAL, and H-FABP may predict hemodynamic relevance of patent ductus arteriosus in very low birth weight infants: V. Tosse, et al.; Neonatology 101, 260 (2012), 摘要; |
38. |
Clinical outcome of renal tubular damage in chronic heart failure: K. Damman, et al.; Eur. Heart J. 32, 2705 (2011), 摘要; |
39. |
Improved performance of urinary biomarkers of acute kidney injury in the critically ill by stratification for injury duration and baseline renal function: Z.H. Endre, et al.; Kidney Int. 79, 1119 (2011), 摘要; |
40. |
Kidney injury molecule-1 and N-acetyl-β-D-glucosaminidase in chronic heart failure: possible biomarkers of cardiorenal syndrome: C.G. Jungbauer, et al.; Eur. J. Heart. Fail. 13, 1104 (2011), 摘要; |
41. |
KIM-1 and NGAL: new markers of obstructive nephropathy: A. Wasilewska, et al.; Pediatr. Nephrol. 26, 579 (2011), 摘要; |
42. |
Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics: B.C. te Boekhorst, et al.; Cardiovasc. Res. 89, 680 (2011), 摘要; |
43. |
Neutrophil gelatinase-associated lipocalin at ICU admission predicts for acute kidney injury in adult patients: H.R. H. de Geus, et al.; Am. J. Respir. Crit. Care Med. 183, 907 (2011), 摘要; |
44. |
Neutrophil gelatinase-associated lipocalin concentrations predict development of acute kidney injury in neonates and children after cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Pediatr. 158, 1009 (2011), 摘要; |
45. |
Renal dysfunction is a stronger determinant of systemic neutrophil gelatinase-associated lipocalin levels than myocardial dysfunction in systolic heart failure: K. Shrestha, et al.; J. Card. Fail. 17, 472 (2011), 摘要; |
46. |
Serum neutrophil gelatinase-associated lipocalin – a sensitive novel marker of renal impairment in liver cirrhosis?: A.L. Gerbes, et al.; Digestion 84, 82 (2011), 摘要; |
47. |
Temporal relationship and predictive value of urinary acute kidney injury biomarkers after pediatric cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Am. Coll. Cardiol. 58, 2301 (2011), 摘要; |
48. |
Tubular markers do not predict the decline in glomerular filtration rate in type 1 diabetic patients with overt nephropathy: S.E. Nielsen, et al.; Kidney Int. 79, 1113 (2011), 摘要; |
49. |
Urinary neutrophil gelatinase-associated lipocalin and progression of diabetic nephropathy in type 1 diabetic patients in a four-year follow-up study: S.E. Nielsen, et al.; Nephron Clin. Pract. 118, c130 (2011), 摘要; |
50. |
Urinary neutrophil gelatinase-associated lipocalin as a marker of acute kidney injury after orthotopic liver transplantation: G. Wagener, et al.; Nephrol. Dial. Transplant. 26, 1717 (2011), 摘要; |
51. |
Urinary neutrophil gelatinase-associated lipocalin at birth predicts early renal function in very low birth weight infants: G. La Manna, et al.; Pediatr. Res. 70, 379 (2011), 摘要; |
52. |
Volume status and diuretic therapy in systolic heart failure and the detection of early abnormalities in renal and tubular function: K. Damman, et al.; J. Am. Coll. Cardiol. 57, 2233 (2011), 摘要; |
53. |
Bile duct ligation in developing rats: temporal progression of liver, kidney, and brain damage: J.M. Sheen, et al.; J. Pediatr. Surg. 45, 1650 (2010), 摘要; |
54. |
Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM1) in patients with diabetic nephropathy: a cross-sectional study and the effects of lisinopril: S.E. Nielsen, et al.; Diabet. Med. 27, 1144 (2010), 摘要; |
55. |
Neutrophil gelatinase-associated lipocalin (NGAL): validation of commercially available ELISA: K.R. Pedersen, et al.; Scand J. Clin. Lab Invest. 70, 374 (2010), 摘要; |
56. |
Pathological significance of a panel of urinary biomarkers in patients with drug-induced tubulointerstitial nephritis: Y. Wu, et al.; Clin. J. Am. Soc. Nephrol. 5, 1954 (2010), 摘要; |
57. |
Possible relationship between neutrophil gelatinase-associated lipocalin, hepcidin, and inflammation in haemodialysed patients: J. Malyszko, et al.; Nephron Clin. Pract. 115, c268 (2010), 摘要; |
58. |
Safety of low volume iodinated contrast administration for arteriovenous fistula intervention in chronic kidney disease stage 4 or 5 utilizing a bicarbonate prophylaxis strategy: E. Eisenhart, et al.; Semin. Dial. 23, 638 (2010), 摘要; |
59. |
Serum neutrophil gelatinase-associated lipocalin correlates with kidney function in heart allograft recipients: P. Przybylowski, et al.; Transplant Proc. 42, 1797 (2010), 摘要; |
60. |
Urinary neutrophil gelatinase-associated lipocalin as a novel biomarker for disease activity in lupus nephritis: T. Rubinstein, et al.; Rheumatology (Oxford) 49, 960 (2010), 摘要; |
61. |
Urinary neutrophil gelatinase-associated lipocalin: A potential biomarker for predicting rapid progression of drug-induced chronic tubulointerstitial nephritis: Y. Wu, et al.; Am. J. Med. Sci. 339, 537 (2010), 摘要; |
62. |
Urinary neutrophil-gelatinase associated lipocalin is a potential noninvasive marker for renal scarring in patients with vesicoureteral reflux: M. Ichino, et al.; J. Urol. 183, 2001 (2010), 摘要; |
63. |
Urine neutrophil gelatinase-associated lipocalin: an independent predictor of adverse outcomes in acute kidney injury: H.N. Yang, et al.; Am. J. Nephrol. 31, 501 (2010), 摘要; |
64. |
Implication of granulocyte-macrophage colony-stimulating factor induced neutrophil gelatinase-associated lipocalin in pathogenesis of rheumatoid arthritis revealed by proteome analysis: M. Katano, et al.; Arthritis Res. Ther. 11, R3 (2009), 摘要; |
65. |
NGAL: a new missing link between inflammation and uremic anemia?: D. Bolignano, et al.; Ren. Fail. 31, 622 (2009), 摘要; |
66. |
Comparison of the effects of gelatin and a modern hydroxyethyl starch solution on renal function and inflammatory response in elderly cardiac surgery patients: J. Boldt, et al.; Br. J. Anaesth. 100, 457 (2008), 摘要; |
67. |
Effect of a single intravenous immunoglobulin infusion on neutrophil gelatinase-associated lipocalin levels in proteinuric patients with normal renal function: D. Bolignano, et al.; J. Investig. Med. 56, 997 (2008), 摘要; |
68. |
Increased incidence of acute kidney injury with aprotinin use during cardiac surgery detected with urinary NGAL: G. Wagener, et al.; Am. J. Nephrol. 28, 576 (2008), 摘要; |
69. |
Neutrophil gelatinase-associated lipocalin as a biomarker of disease activity in pediatric lupus nephritis: M. Suzuki, et al.; Pediatr. Nephrol. 23, 403 (2008), 摘要; |
70. |
Neutrophil gelatinase-associated lipocalin expression in chronic myeloid leukemia: C. Villalva, et al.; Leuk. Lymphoma 49, 984 (2008), 摘要; |
71. |
Neutrophil gelatinase-associated lipocalin reflects the severity of renal impairment in subjects affected by chronic kidney disease: D. Bolignano, et al.; Kidney Blood Press. Res. 31, 255 (2008), 摘要; |
72. |
NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?: H. Bachorzewska-Gajewska, et al.; Int. J. Cardiol. 127, 290 (2008), 摘要; |
73. |
NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?: H. Bachorzewska-Gajewska, et al.; Int. J. Cardiol. 127, 290 (2008), 摘要; |
74. |
Serum neutrophil gelatinase-associated lipocalin as a marker of renal function in non-diabetic patients with stage 2-4 chronic kidney disease: J. Malyszko, et al.; Ren. Fail. 30, 625 (2008), 摘要; |
75. |
Urinary neutrophil gelatinase associated lipocalin (NGAL), a marker of tubular damage, is increased in patients with chronic heart failure: K. Damman, et al.; Eur. J. Heart Fail. 10, 997 (2008), 摘要; |
76. |
Urinary neutrophil gelatinase-associated lipocalin (NGAL) is associated with severity of renal disease in proteinuric patients: D. Bolignano, et al.; Nephrol. Dial. Transplant. 23, 414 (2008), 摘要; |
77. |
Urinary neutrophil gelatinase-associated lipocalin and acute kidney injury after cardiac surgery: G. Wagener, et al.; Am. J. Kidney Dis. 52, 425 (2008), 摘要; |
78. |
Urinary NGAL in premature infants: A.P. Lavery, et al.; Pediatr. Res. 64, 423 (2008), 摘要; |
79. |
Urine neutrophil gelatinase-associated lipocalin and interleukin-18 predict acute kidney injury after cardiac surgery: C. Xin, et al.; Ren. Fail. 30, 904 (2008), 摘要; |
80. |
Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study: M. Bennett, et al.; Clin. J. Am. Soc. Nephrol. 3, 665 (2008), 摘要; |
81. |
Could neutrophil-gelatinase-associated lipocalin and cystatin C predict the development of contrast-induced nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine values?: H. Bachorzewska-Gajewska, et al.; Kidney Blood Press. Res. 30, 408 (2007), 摘要; |
82. |
Detection of subclinical tubular injury after renal transplantation: comparison of urine protein analysis with allograft histopathology: S. Schaub, et al.; Transplantation 84, 104 (2007), 摘要; |
83. |
Neutrophil gelatinase-associated lipocalin (NGAL) correlations with cystatin C, serum creatinine and eGFR in patients with normal serum creatinine undergoing coronary angiography: H. Bachorzewska-Gajewska, et al.; Nephrol. Dial. Transplant. 22, 295 (2007), 摘要; |
84. |
Neutrophil gelatinase-associated lipocalin in patients with autosomal-dominant polycystic kidney disease: D. Bolignano, et al.; Am. J. Nephrol. 27, 373 (2007), 摘要; |
85. |
Neutrophil-gelatinase-associated lipocalin and renal function after percutaneous coronary interventions: H. Bachorzewska-Gajewska, et al.; Am. J. Nephrol. 26, 287 (2006), 摘要; |
其他参考文献
1. |
Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), 摘要; |
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