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“AddexBio细胞株SJSA-1cells(骨肉瘤;多潜能肉瘤)”
疾病: 骨肉瘤;多潜能肉瘤
来源: 原发性股骨的原始多向肉瘤
种类: 人
组织: 骨头
Disease:
Osteosarcoma; multipotential sarcoma
Origin:
Derived from primary tumor of a patient diagnosed with primitive multipotential sarcoma of the femur
Species:
Homo sapiens
Tissue:
Bone
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene
Patient:
Male, Black, 19 years of age
Medium:
RPMI1640 + 10% FBS
Subculture:
1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 degree C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Isoenzymes:
Not known
DNA Profile (STR):
Not known
Mutations in SJSA-1 Cells
References for SJSA-1 cells:
1. Bransfield, K. L., Askham, J. M., Leek, J. P., Robinson, P. A., and Mighell, A. J. Phenotypic changes associated with DYNACTIN-2 (DCTN2) over expression characterise SJSA-1 osteosarcoma cells. Mol Carcinog, 45: 157-163, 2006.
2. Missirlis, D., Khant, H., and Tirrell, M. Mechanisms of peptide amphiphile internalization by SJSA-1 cells in vitro. Biochemistry, 48: 3304-3314, 2009.
3. Missirlis, D., Krogstad, D. V., and Tirrell, M. Internalization of p53(14-29) peptide amphiphiles and subsequent endosomal disruption results in SJSA-1 cell death. Mol Pharm, 7: 2173-2184.
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