Clontech逆转录病毒载体

pRetroX-IRES-ZsGreen1

Description

pRetroX-IRES-ZsGreen1 is a bicistronic, fl uorescent, retroviral vector that allows both a gene 

of interest and the ZsGreen1 gene to be translated from a single bicistronic mRNA. pRetroXIRES-ZsGreen1 is designed for effi cient delivery and selection （by fl ow cytometry or other 

methods） of stably transduced mammalian cells expressing the ZsGreen1 fl uorescent protein 

and the protein of interest. This vector can be used to obtain stable cell lines without timeconsuming drug and clonal selection. ZsGreen1 is derived from the reef coral Zoanthus sp. 

green fl uorescent protein （ZsGreen） and is easily detected with standard FITC fi lter sets （1）.

Bicistronic expression from this vector is facilitated by the encephalomyocarditis virus （EMCV） 

internal ribosome entry site （IRES）. This IRES facilitates cap-independent translation from an 

internal start site at the IRES/ZsGreen1 junction （2）. ZsGreen1 is a human codon optimized 

ZsGreen variant that encodes the brightest commercially available green fl uorescent protein 

（1）. This retroviral vector is derived from the pMIN series of vectors （3, 4）. These optimized 

vectors have the ability to produce high viral titers, express genes at high levels, and, due 

to the absence of retroviral coding sequences, exhibit improved safety profi les. The multiple 

cloning site （MCS） in pRetroX-IRES-ZsGreen1 is between the 5’ MMLV LTR and the IRES 

sequence. Genes cloned into the MCS are expressed as a bicistronic message transcribed 

from the 5’ LTR.

pRetroX-IRES-ZsGreen1 contains all of the necessary viral RNA processing elements; these 

include the 5’ and 3’ LTRs, the packaging signal （ψ）, and the tRNA primer-binding site. For 

safety reasons, however, the vector lacks the structural genes （gag, pol, and env） necessary for 

retroviral particle formation and replication. pRetroX-IRES-ZsGreen1 contains a ColE1 origin 

of replication, and an E. coli Ampr

 gene for propagation and selection in bacteria.