世界*实验材料供应商 Sanquin 正式上海金畔为其中国代理， Sanquin 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔一直秉承为中国科研客户带来的产品，的服务， Sanquin 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
Sanquin 中国代理, Sanquin 上海代理， Sanquin 北京代理，Sanquin 广东代理， Sanquin 江苏代理Sanquin 湖北代理,Sanquin 天津,Sanquin 黑龙江代理,Sanquin 内蒙古代理,Sanquin 吉林代理,Sanquin 福建代理, Sanquin 江苏代理, Sanquin 浙江代理, Sanquin 四川代理,
Sanquin是通过荷兰血库和荷兰红十字会输血服务中心实验室之间的合并，成立于1998年（CLB）。公司成立基金会负责的血液供应，以非营利为基础提出输血等，符合zui高质量要求，安全性和效率。Sanquin为科学研究和提供教育，教学，培训。Sanquin血液供应的基础保证安全有效的在荷兰的血液供应。Sanquin还开发并生产医药产品，进行高质量的科学研究，开发和执行多种诊断服务。
Sanquin human IgG subclass Enzym Linked Immuno Sorbent Assay (ELISA)
Sanquin’s enzyme-linked immunosorbent assay is a sandwich type immunoassay. The kit contains microwell strips coated with highly avid monoclonal antibodies.
Principle
Test samples, calibrator and control sera are incubated in the wells. The IgG subclass to be determined binds to the solid phase and non-bound IgG is removed by washing.
Next peroxidase-conjugated anti-human IgG antiserum is added and non-bound conjugate is removed by washing. After incubation with substrate solution (ABTS) and H2O2 the reaction is stopped with an acid buffer.
The green coloured reaction product is measured by absorption and the concentration of the IgG subclass is calculated relatively to the values of a reference curve.
The IgG subclass concentrations in the calibrators were determined using a calibrator derived from the WHO 67/97 reference preparation.
Content of the ELISA kit
The kit contains 48 tests, including calibrators and controls, for each of the four subclasses:
• 2 precoated microtiter plates
• HRP conjugated a-IgG antibodies
• standard and control sera
• wash, dilution, substrate and stop buffers
• ABTS and hydrogen peroxidase substrate
Product number of the kit is M1551.
510(k) number: 943098