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Hypoxyprobe™ F6 Kit

HPI Catalog # HP4-XXX

Kit Contents:

Solid CCI-103F (Hypoxyprobe™-F6)

Diluted Rabbit anti-CCI-103F antisera (PAbF6)

Applications: Use the Hypoxyprobe™-F6 Kit to measure hypoxia in tissues and cells by means of immunochemical detection including immunoperoxidase, immunofluorescence or flow cytometry. (Kleiter et al., Int. J. Radiation Oncol. Biol. Phys. 64(2): 592-602, 2006). Ljungkvist et al., Int. J. Radiat. Oncol. Biol. Phys. 62(4): 1157-1168, 2005).

Quantities: a. Hypoxyprobe™-F6 Kits contain 100, 200 or 1000 mg, respectively, of CCI-103F (Hypoxyprobe™–F6). Typical doses are 60mg/kg body weight is used in small animal studies.

b. Each Kit contains diluted anti-CCI-103F rabbit antiserum (PAbF6) containing 0.09% sodium azide and 1% BSA as stabilizers. The 100 and 200 mg Kits contain one 200 uL unit of antiserum; the 1000 mg Kit contains two 200 uL units of antiserum. Optimal operating dilutions of the antisera are to be determined by investigators but 1/200 -1/500 dilutions give strong immunoperoxidase staining when combined with peroxidase conjugated goat anti-rabbit secondary antisera.

Not supplied: Anti-rabbit secondary reagents or other immunohistochemical reagents (buffers, antigen retrieval agents, etc).

Storage: a. Store Hypoxyprobe™–F6 in the dark at room temperature (can also be stored at 2-8 degrees C)

b. Store rabbit antisera (PAbF6) at 2-8 degrees C.

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