试剂盒成分
● 2 瓶 – microRNA Reprogramming 混合物Ⅰ(20 μM)(货号 05-0036),35 μL
● B18R 重组蛋白,不含载体(货号. 03-0017),50 μg
保存和保质期 Storage and Stability
● 将 microRNA 重编程混合物和 B18R 蛋白保存在 -70℃以下。
● 按照指示的保存,收到货后至少可以保存3个月。
质量控制
Stemgent® microRNA Booster 试剂盒用人新生的包皮成纤维细胞成功进行基于 RNA 重编程。所有试剂盒组分都灭过菌,检测不含支原体。
推荐使用
● Stemgent® microRNA Booster Kit 用于 microRNA-增强型 mRNA 重编程
● 参考制备 NuFF-条件性 Pluriton 培养基用于制备条件性重编程培养基。
● 参考操作手册: Stemgent® mRNA 重编程系统,关注实验细节和材料处理。
Specification Sheets
00-0073 Product Specification Sheet
Safety Data Sheets
03-0017 Safety Data Sheet
00-0073 Safety Data Sheet
05-0036 Safety Data Sheet
Protocols
microRNA Enhanced mRNA Reprogramming
Application Notes
MicroRNA Application Note
Product Sheets
Product Sheet_RNA Reprogramming
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mRNA Reprogramming Factors Set: hOKSML (00-0067)
Additional Publications
7q11.23 dosage-dependent dysregulation in human pluripotent stem cells affects transcriptional programs in disease-relevant lineages
Josowitz R, Lu J, Falce C, D’Souza SL, Wu M, Cohen N, Dubois NC; Zhao Y; Sobie EA; Fishman GI; Gelb BD. Identification and purification of human induced pluripotent stem cell-derived atrial-like cardiomyocytes based on sarcolipin expression. PLoS ONE 9(7): e101316 (2014)
Briggs SF; Dominguez AA; Chavez SL; Reijo Pera RA. "XIST in human preimplantation embryos and iPSCs." Stem Cells 33:1771 (2015)