上海金畔生物科技正规代理Trilink产品，zui近市场出现Trilink的假货，为了您的实验安全请选择正牌代理。 :

TriLink BioTechnologies——高品质常规/特殊核苷酸产品
TriLink BioTechnologies公司是世界的核酸修饰技术领域的，成立于1996年，总部设在San Diego，California。TriLink公司致力于高品质核酸修饰产品的研发和生产，提供包括核苷酸、常规及核苷三磷酸特殊修饰的寡核苷酸定制（oligonucleotides），m RNA合成，CleanAmp?PCR产品，phosphoramidites和其他小分子在内的众多产品。近二十年来，TriLink一直是诊断和OEM市场核酸产品供应的行业，产品应用于基因治疗，核苷类化疗，寡核苷酸治疗和诊断领域。此外，TriLink还可提供特殊核苷酸、m RNA定制，合同研究服务和ISO/ QSR标准的cGMP生产设施。TriLink完善的产品及研发服务解决方案有助于推动药物发现和生物医学研究。

经过18年的发展， TriLink已经成为高品质RNA合成领域的，为广大科研工作者提供的mRNA及长链RNA（长可达几个Kb），并且定制修饰。同时我们也提供成品mRNA产品，包括报告基因及 mRNA表达因子。

Antigen mRNA for Vaccines and Immunotherapy

mRNA offers several advantages over traditional plasmid and viral-based approaches:

• mRNA boasts a superior safety profile. As a transient carrier of genetic information, it is metabolized naturally and poses little to no risk of genomic integration. Additionally, no inactivated viruses or pathogens are needed.
• mRNA serves the dual purpose of expressing the desired antigen as well as acting as an adjuvant.
• mRNA triggers a more diverse immune response. Because the mRNA encoded epitopes are intracellular, they are recognized by the immune system in an MHC class-independent manner.
• mRNA can more readily transfect difficult-to-transfect cell types because it functions in the cytoplasm. DNA vaccines can be limited by lack of access to the nucleus.
• mRNA manufacturing is easily scalable. Because mRNA transcription is carried out compley in vitro, to hundreds of millions of vaccine doses with a lead time of as little as a few weeks. This allows for rapid deployment of a new antigen during pandemics.
• mRNA is easily customizable. The ease of manufacturing makes it a viable option for personalized treatments.

产品如下:

L-7210
CleanCap™ OVA mRNA (5moU)
CleanCap™ Ovalbumin mRNA (5-methoxyuridine) 

mRNA Length: 1,437 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Insert
SDS

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunization and biochemical studies and is an established model allergen for airway hyper-responsiveness.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

TriLink offers both unmodified and 5-methoxyuridine modified OVA mRNA. Exogenous unmodified mRNA activates the innate immune system and production of cytokines, which will influence the overall induced immune response. mRNA modified with 5-methoxyuridine reduces this effect.
 

Certificate(s) of Analysis

TD-OB06A

L-7210 is a replacement for L-6128,

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

L-7610
CleanCap™ OVA mRNA
CleanCap™ Ovalbumin mRNA 

mRNA Length: 1,437 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Insert
SDS

Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunization and biochemical studies and is an established model allergen for airway hyper-responsiveness.

This mRNA is capped using CleanCap™, TriLink’s proprietary co-transcriptional capping method,

which results in the naturally occurring Cap 1 structure with high capping efficiency. It is polyadenylated and optimized for mammalian systems. It mimics a fully processed mature mRNA.

TriLink offers both unmodified and 5-methoxyuridine modified OVA mRNA. Exogenous unmodified mRNA activates the innate immune system and production of cytokines, which will influence the overall induced immune response. mRNA modified with 5-methoxyuridine reduces this effect.

Certificate(s) of Analysis

TD-OB07A

L-7610 is a replacement for L-6328.

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

上海金畔生物科技正规代理Trilink产品，zui近市场出现Trilink的假货，为了您的实验安全请选择正牌代理。 :

TriLink BioTechnologies——高品质常规/特殊核苷酸产品
TriLink BioTechnologies公司是世界的核酸修饰技术领域的，成立于1996年，总部设在San Diego，California。TriLink公司致力于高品质核酸修饰产品的研发和生产，提供包括核苷酸、常规及核苷三磷酸特殊修饰的寡核苷酸定制（oligonucleotides），m RNA合成，CleanAmp?PCR产品，phosphoramidites和其他小分子在内的众多产品。近二十年来，TriLink一直是诊断和OEM市场核酸产品供应的行业，产品应用于基因治疗，核苷类化疗，寡核苷酸治疗和诊断领域。此外，TriLink还可提供特殊核苷酸、m RNA定制，合同研究服务和ISO/ QSR标准的cGMP生产设施。TriLink完善的产品及研发服务解决方案有助于推动药物发现和生物医学研究。

经过18年的发展， TriLink已经成为高品质RNA合成领域的，为广大科研工作者提供的mRNA及长链RNA（长可达几个Kb），并且定制修饰。同时我们也提供成品mRNA产品，包括报告基因及 mRNA表达因子。

Reporter Gene mRNA

Reporter genes are commonplace tools in cell biology research. TriLink has developed optimized capped (Cap 0 and Cap 1) and polyadenylated mRNA expressing reporters. These reporter mRNAs can be used as controls to study mRNA transfection and expression in mammalian cells using a variety of assays including fluorescence microscopy, quantitative fluorometry and bioluminescent imaging, as well as fluorescence-activated cell sorting (FACS).

产品如下：

L-7601
CleanCap™ EGFP mRNA
CleanCap™ Enhanced Green Fluorescent Protein mRNA 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product InsertSDS

The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Sold for research use only under license from Life Technologies.

Certificate(s) of Analysis

TD-OF01A

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

L-7201
CleanCap™ EGFP mRNA (5moU)
CleanCap™ Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Inser

SDS         

The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Certificate(s) of Analysis

TD-OA01A-1

TD-OA01A

T1-CHI01A-1

L-7201 is a replacement product for L-6101 and L-6126.

Sold for research use only under license from Life Technologies.

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

L-7701
CleanCap™ Cyanine 5 EGFP mRNA (5moU)
CleanCap™ Cyanine 5 Enhanced Green Fluorescent Protein mRNA (5-methoxyuridine) 

mRNA Length: 996 nucleotides

Concentration: 1.0 mg/mL

Buffer: 1 mM Sodium Citrate, pH 6.4

Identity & Purity:

Agarose Gel Mobility; Pass

Concentration ± 5%; Pass

Product Insert

SDS

Cyanine 5 EGFP mRNA is designed for the analysis of mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. When labeled with cyanine 5, EGFP mRNA can be directly visualized. Cyanine 5 EGFP mRNA is an ideal molecule to determine mRNA delivery and localization independent of translation.

Cyanine 5 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 650 nm and 670 nm respectively. TriLink’s CleanCap Cyanine 5 EGFP mRNA is transcribed with Cyanine 5-UTP:5-Methoxy-UTP at a ratio of 1:3. Substitution in this ratio results in mRNA that is easily visualized and can still be translated in cell culture. Translation efficiency correlates inversely with Cyanine 5-UTP substitution.

This mRNA is capped using CleanCap™, TriLink's proprietary co-transciptional capping method, which results in the naturally occuring Cap 1 structure with high capping efficiency. It is polyadenylated, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics a fully processed mature mRNA.

Certificate(s) of Analysis

TD-OF03A

L-7701 is a replacement for L-6402. 

Sold for research use only under license from Life Technologies.

Not for resale without express written permission. Not for use in humans. No license under any patent or patent pending is granted or implied by the purchase of any TriLink product. TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser’s sole responsibility.

TriLink BioTechnologies——高品质常规/特殊核苷酸产品

TriLink BioTechnologies公司是世界的核酸修饰技术领域的，成立于1996年，总部设在San Diego，California。TriLink公司致力于高品质核酸修饰产品的研发和生产，提供包括核苷酸、常规及核苷三磷酸特殊修饰的寡核苷酸定制（oligonucleotides），m RNA合成，CleanAmp™PCR产品，phosphoramidites和其他小分子在内的众多产品。近二十年来，TriLink一直是诊断和OEM市场核酸产品供应的行业，产品应用于基因治疗，核苷类化疗，寡核苷酸治疗和诊断领域。此外，TriLink还可提供特殊核苷酸、m RNA定制，合同研究服务和ISO/ QSR标准的cGMP生产设施。TriLink完善的产品及研发服务解决方案有助于推动药物发现和生物医学研究。

公司主要产品：

一、寡核苷酸产品

近二十年来，TriLink一直是诊断和OEM市场核酸产品供应的行业，产品应用于基因治疗，核苷类化疗，寡核苷酸治疗和诊断领域。公司提供任意选择的DNA、RNA 以及经修饰的寡核苷酸产品，包括常规产品、荧光染料/非荧光染料标记产品、特殊修饰产品、5端加帽产品等。 无论你需要的是任何标准DNA或RNA寡核苷酸，还是特殊的复杂序列，我们都将为您合成满足您实验需要的量及纯度的核苷酸产品。我们的每批产品均经过包括PAGE, HPLC和/或质谱分析以保证其品质。我们将以高品质产品服务于科研、临床前/临床、诊断领域。

二、核苷酸产品：

TriLink提供多种多样的核苷酸产品，适宜于配体研究、抗病毒核苷酸研究、凋亡研究、染色体绘图、药物研发、表观遗传研究、热启动PCR反应、基因测序、RNA分离与绘图、干细胞重编程、mRNA 修饰及治疗研究。

三、DNA合成试剂：

该产品系列包括以下产品：Phthalimidyl 3'-C6-Amino-CPG (500 Å)、 Phthalimidyl 3'-C3-Amino-CPG (500 Å)、Propyl-CPG (500 Å)、Dabcyl-CPG (500 Å)、DMT-C6-Disulfide-CPG (500 Å/1000 Å)、3 Å Molecular Sieves、CleanAmp™ Amidite: PAC-dA-CE、CleanAmp™ Amidite: PAC-dG-CE、CleanAmp™ Amidite: Ac-dC-CE、CleanAmp™ Amidite: dT-CE

四、CleanAmp™ PCR产品

CleanAmp™的热启动PCR是TriLink在SBIR资助下由NIH购得的产品，该产品含有一种高灵敏度、高特异性及高灵活度的热启动酶。 TriLink利用其在修饰核苷酸领域的优势开发出了修饰的dNTPs及引物，这些引物能在常规 DNA Taq酶下实现热启动。只需简单的替换掉PCR反应中的常规dNTPs或引物， 即可在CleanAmp™ 产品下进行热启动的PCR反应。CleanAmp™产品通过在模板处于较低浓度的初始循环中限制激活试剂的量，从而减少北京扩增，进而减少甚至消除引物二聚体及错配的产生。CleanAmp™ 系列产品适用于RT-PCR, 多重 PCR, 数字PCR, 快速PCR, end-point PCR 及 real-time PCR (包括快速real-time PCR)。 CleanAmp™ 热启动PCR试剂与所有DNA聚合酶及PCR反应兼容、具有的特异性及灵敏性、产量高、操作简便、扩增效率高（23kb以上仍然维持高扩增效率）、 高性价比。

五、mRNA 及 Long RNA

经过18年的发展， TriLink已经成为高品质RNA合成领域的，为广大科研工作者提供的mRNA及长链RNA（长可达几个Kb），并且定制修饰。同时我们也提供成品mRNA产品，包括报告基因及 mRNA表达因子。

六、 早期治疗GMP产品

在这20年中，我们已经成长为高品质研究及诊断级别GMP车间产品，以及GMP标准生产的mRNA及寡核苷酸治疗产品，特别是不常见的复杂结构化合物，范围包括毒理研究、早期临床研究类产品。

七、适配体产品

自1996年以来， TriLink′s在修饰核苷酸合成领域的优势就已经为适配体研究奠定了坚实的基础。 目前TriLink 通过提供用于SELEX的经修饰核苷三磷酸产品、常备的包括DNA/RNA的随机文库以及核酸适配体的合成及交联服务于产品。

八、表观遗传学研究试剂

近年来表观遗传修饰对基因表达的影响已经引起越来越多的重视， 特别是DNA的甲基化，现在，在表观遗传学中起着重要作用的新碱基修饰已经被陆续发现，如5-Hydroxymethyl-dU, 5-Hydroxymethyl-dC，5-Formyl-dC。为服务于这一前沿领域的研究，TriLink提供包括核苷特殊修饰的DNA、RNA以及寡核苷酸产品， 并且是目前*一家能生产众多新发现及难以合成的碱基修饰核酸产品的供应商。该产品系列目录如下：

九、线粒体DNA (mtDNA)分析试剂

TriLink 高品质的核酸合成技术使其成为mtDNA研究分析当之无愧的引物合成供应商。 TriLink公司的mitoPrimers™ 是一系列扩增人类线粒体DNA超变量区1/2  (HV1/HV2)的特异引物，并大大简化了分析步骤，节省大量宝贵时间。目前TriLink还提供多款线粒体DNA测序、SNP检测用产品及试剂盒，可广泛用于学术研究、法医鉴定（失踪人口及灾难后牺牲者身份确认）等领域，高度腐化的样本及毛发、骨骼、牙齿、指甲及血样均可用于线粒体DNA分析。

十、定制服务

为满足部分研究人员特殊的实验需求，TriLink还提供市场难以采购到的特殊修饰的核酸产品、生物小分子定制合成服务。