worthington LK003150现货说明书

上海金畔worthington LK003150现货说明书

Papain Dissociation System

沃辛顿木瓜蛋白酶解离系统是一套用于Huettner和Baughman的神经细胞分离方法的试剂(J.Neurosci.,63041986)。这些材料的设计是为了方便和简单,对偶尔使用的用户以及更有经验和频繁使用的用户都很有用。每个批次都用于测试大鼠脊髓神经细胞分离的性能,该试剂盒为每次分离提供新鲜制备的酶溶液。

稳定性/储存:试剂在环境温度下在正常运输程序中预期的时间内是稳定的,但包装应在到达时冷藏。使用前,内容物可在2-8°C下储存4-6个月。储存于2-8°C。

包装内容

该包装包含足够的材料,用于分离五个单独的组织等分试样,每个试样的体积高达0.3-0.4 cm3。对于更大的组织样品,在每个步骤中按比例制备更大体积的试剂,并按照方案中所述的相同比例组合它们。

•小瓶1:含有钙、镁、碳酸氢盐和酚红的无菌Earl's平衡盐溶液(EBSS),每个包装一小瓶,100ml。该小瓶的等分试样用于重建其他小瓶并制备稀释抑制剂溶液。使用之间冷藏,每次使用前用无菌O2:CO2平衡。

•小瓶2:含L-半胱氨酸和EDTA的木瓜蛋白酶,每个包装5 x 100单位一次性小瓶。该材料经0.22微米膜过滤并在高压釜小瓶中冷冻干燥。用5ml EBSS(1号小瓶)重新配制的小瓶产生每毫升20单位木瓜蛋白酶在1mM L-半胱氨酸和0.5mM EDTA中的溶液。需要在37°C下短暂孵育,以确保溶解和活性。

•小瓶3:脱氧核糖核酸酶I(DNase),每个包装5 x 2000单位一次性小瓶。

该材料经0.22微米膜过滤并在高压釜小瓶中冷冻干燥。用0.5ml EBSS(1号小瓶)重新配制的小瓶产生每毫升2000单位脱氧核糖核酸酶的溶液。避免剧烈混合。

•小瓶4:含牛血清白蛋白的卵母细胞蛋白酶抑制剂,每包一小瓶,重组后32ml。

该材料经0.22微米膜过滤并在高压釜小瓶中冷冻干燥。用32ml EBSS(1号小瓶)重新配制的小瓶产生有效浓度为10mg卵粘液抑制物和10mg白蛋白/ml的溶液。每次解离使用该小瓶的等分试样。使用之间冷藏,每次使用前用无菌O2:CO2平衡。当储存在2-8°C时,重构后稳定。

还包括一张与pH值相关的卡片,用作O2:CO2平衡的指南。

相关产品:

胶原酶(CLS1-4/CLSA)

脱氧核糖核酸酶I(DP/D/DCLS/D2/DPFF/DPRF)

透明质酸酶(HSE/HSEP)

中性蛋白酶(Dispase,NPRO)

蛋白酶K(PROK)

胰蛋白酶(TL/TRL/TRL3/TRLS/TRTPCK)

胰蛋白酶抑制剂(LBI/OI/SI/SIC)

细胞分离优化系统(CIT)

肝细胞分离系统(HIS)

新生儿心肌细胞分离系统(NCIS)

木瓜蛋白酶解离系统

来源:不适用

一套五瓶一次性木瓜蛋白酶和五瓶一次性DNase、100ml厄尔平衡盐溶液(EBSS)和一瓶抑制剂,用于Huettner,J.E.和Baughman,R.W.:J.Neuroscience的组织分离方法。沃辛顿使用新生大鼠幼崽脊髓进行了使用测试。该包装包含足够的材料,用于分离五个单独的组织等分试样,每个试样的体积高达0.3-0.4 cm3。

储存于2-8°C。

货号:LK003150

品牌:worthington

worthington LK003150现货

淋巴细胞分离简述

作为一种重要的免疫细胞,淋巴细胞的数目及功能状态反应了机体所处的免疫状态,与一些先天或后天的免疫缺陷性疾病、传染性疾病以及恶性肿瘤等免疫功能障碍有关的疾病有密切关联。因此,研究淋巴细胞的免疫状态对多种疾病的诊断、预防及治疗都有着极为重要的意义。而淋巴细胞分离是研究淋巴细胞功能状态及其在细胞免疫中作用的关键步骤。

淋巴细胞分离来源主要是外周血(或淋巴组织细胞悬液),目前常规的淋巴细胞分离方法有基于淋巴细胞分离液的密度梯度离心法、HES沉降法、以及基于抗原抗体结合的磁珠与流式细胞分离法。本文就现在常用的几种淋巴细胞分离方法及其特点做一简要综述。

1、密度梯度离心法

该方法采用淋巴细胞分离液进行进行密度梯度离心分离得到淋巴细胞。外周血单个核细胞(peripheral blood mononuclear cell,PBMC)包括淋巴细胞和单核细胞,其体积、形状和比重与其他细胞不同从而导致各种细胞具有不同的密度,淋巴细胞分离液即是一种根据细胞密度差异,借助离心产生的重力加速度,使一定密度的细胞按相应密度梯度分布,从而将各种血细胞加以分离纯化的常用试剂。常用的淋巴细胞分离液有Ficoll淋巴细胞分离液和Percoll淋巴细胞分离液。

1.1. Ficoll-Hypaque密度梯度离心法

Ficoll是一种中性的蔗糖的多聚体,吸水性强,平均分子量为400,000,具有高密度、低渗透压、无毒性的特点。单一的Ficoll溶液分子量大,高浓度时增加粘度易导致细胞聚集,因此常用的Ficoll淋巴细胞分离液采用了降低Ficoll浓度,同时加入泛影葡胺增加密度的Ficoll-Hypaqu混合溶液,其密度在1.077左右,近于等渗,适用于分离外周血淋巴细胞和单个核细胞(PBMC,外周血淋巴细胞和单个核细胞比重为1.075左右)1。利用该分离液作密度梯度离心时,各种血液成分将按密度梯度重新分布聚集,红细胞与粒细胞由于密度较大故沉于分层液的底部,淋巴细胞和单核细胞(PBMC)的比重与分层液比重相近,故位于分层液界面上。23

 Ficool淋巴细胞分离液是一种经典的淋巴细胞分离试剂,分离得到的PBMC纯度在90%以上,收获率可达8090%,活细胞百分率在95%以上。然而因其固定的密度,故只适用于PBMC、脊髓干细胞、骨髓干细胞的分离。

订货信息:

供应商               品名                    货号        规格

MP Biomedicals   Lymphocyte Separation Medium – LSM?  0850494X    100 mL  

biomarket       LymphoprepTM分离液          1114544      250ml

 

1.2. Percoll密度梯度离心法:

Percoll成分为硅化聚乙烯吡咯烷酮(PVP)的颗粒,渗透压很低,粘度也很小,不穿透生物膜,对细胞无毒害无刺激,不引起细胞聚集,可形成高达1.3gml密度。特别的是percoll的颗粒直径大小不同,可在离心过程中自然形成连续的密度梯度,从而将不同密度的细胞分离出来。而且Percoll扩散常数低,所形成的梯度十分稳定,配置不同浓度(密度)时仅需通过用PBS(1x)或组织培养液稀释即可获得,广泛用于分离细胞、亚细胞成分、细菌及病毒,还可将受损细胞及其碎片与完好的活细胞分离。采用Percoll细胞分离液得到的细胞可直接用于基于荧光标记的细胞分选。4

因此,Percoll细胞分离液不仅适用于外周血中PBMC的分离(单个核细胞和淋巴细胞分别处于不同的密度层),而且能将全血中的多种细胞分离出来(各种细胞处于相应的梯度层),还可根据不同实验室需要配置所需的连续密度梯度,分离特定的细胞或亚细胞组分。初步分离得到的PBMC可根据需要不同密度梯度的不连续梯度离心方法进行进一步细胞纯化,如纯化淋巴母细胞和除去死细胞以及富含NK活性大颗粒淋巴细胞(LGL)的纯化。5

常用percoll浓度密度换算(Percoll原液与10倍浓缩的PBS91的比例混合,此时溶液 为100 Percoll,比重是1.127

Percoll浓度()    70       60       50       40       30        20

比重gml       1.090  1.077    1.067   1.056   1.043     1.031

订货信息:

供应商    货号           规格

GE    17-0891-01         1 L

GE    17-0891-01         100 ml

 

1.3. Iodixanol梯度离心法

Iodixanol是一种化学成分为碘海醇(Iohexo1)的白色、高亲水性粉剂,可以配成型的非离子密度梯度分离液。分子量为821,密度为2.1 g/ml,是非离子型的、对细胞无毒性的化合物,可用于细胞、细胞器、生物大分子、病毒等各种生物物质的分离或提纯6。该分离液的密度可以通过测定其折射率来确定,配置后可以进行高温高压的灭菌。可根据需要配置成不同密度的梯度离心液,主要用于脂蛋白的分离,也可用于淋巴细胞分离纯化。7,8

 

订货信息:

供应商              品名                        货号           规格

Axis-Shield      Nycodenz(粉剂)         AS1002424      1x500g

Axis-Shield  LymphoprepTM  分离管(即用型)   AS1019818    18Tubes x10ml

Axis-Shield   OptiPrepTM  分离液(即用型)AS1114542      1x250ml

Axis-Shield  LymphoprepTM  分离液(即用型)AS1114544    1x250ml

Axis-Shield  LymphoprepTM  分离液(即用型)AS1114545    4x250ml

Axis-Shield  LymphoprepTM  分离液(即用型)AS1114547    1x500ml

Axis-Shield  LymphoprepTM  分离液(即用型)AS1114547     6x500ml

Axis-Shield  NycoPrepTM 1.077  分离液(即用型)AS1114550  4x250ml

Axis-Shield  NycoPrepTM 1.077  分离液(即用型)AS1114551   1x250ml

 

2、淀粉(HES)离心沉淀法

HES是一种由高分子量支链淀粉经降解进一步加工处理后制成的血浆代用品9。自然状态下,HES与红细胞膜上的负电荷结合,使红细胞彼此以凹面相贴聚集而下沉,与血浆及单个核细胞形成清晰的界面。如果HES与外周血混合物经低速离心可加快红细胞的下沉,便于吸取含单核细胞层,使PBMC的分离过程简单化。该方法分离PBMC操作简便,成本低廉,分离细胞数量与密度梯度离心方法相当。然而该方法分离的细胞质量有待进一步提高。10-12

订货信息:

国内多家血浆代用品供应商如湖北武汉康宝泰精细化工有限公司可以获得。

 

3、基于免疫技术的淋巴细胞分离法

目前多家生物制品公司均开发出的基于抗原抗体特异性结合的淋巴细胞分离试剂盒,主要有免疫磁珠分离法、流式细胞分选法13,14。这些试剂盒能特异性分离外周血或其它组织中的单一淋巴细胞类型,操作简便,所得细胞纯度高,可直接用于下游分子生物学实验。然而该法基于抗体技术,因此成本高,还未成为目前的主流淋巴细胞分离方法。

订货信息

供应商              品名                              货号

Stem cell   EasySep? Magnet全淋巴细胞富集试剂盒    19961HLA

Stem cell   EasySep? MagnetT细胞富集试剂盒         19951HLA

Stem cell   EasySep? MagnetB细胞富集试剂盒         19954HLA

.

主要参考文献:

1. Separation of human lymphocytes from citrated blood by density gradient (NycoPrep) centrifugation: monocyte depletion depending upon activation of membrane potassium channels. B?yum A, Brincker Fjerdingstad H, Martinsen I, Lea T, L?vhaug D. Scand J Immunol. 2002 Jul;56(1):76-84.

2. Separation of leucocytes: improved cell purity by fine adjustments of gradient medium density and osmolality. B?yum A, L?vhaug D, Tresland L, Nordlie EM. Scand J Immunol. 1991 Dec;34(6):697-712.

3. Isolation of lymphocytes, granulocytes and macrophages. B?yum A.

Scand J Immunol. 1976 Jun;Suppl 5:9-15.

4. A simple method for human peripheral blood monocyte isolation. de Almeida MC, Silva AC, Barral A, Barral Netto M. Mem Inst Oswaldo Cruz. 2000 Mar-Apr;95(2):221-3

5. Isolation of brain and spinal cord mononuclear cells using percoll gradients.

Pino PA, Cardona AE. J Vis Exp. 2011 Feb 2;(48). pii: 2348.

Mem Inst Oswaldo Cruz. 2000 Mar-Apr;95(2):221-3.

6. Iodixanol梯度离心法分离脂蛋白. 党美林,林元喜. 《第七届海峡两岸心血管科学研讨会论文集》.2009

7. Su-Ting Chen, Jia-Yun Li, Yi Zhang. Recombinant MPT83 Derived from Mycobacterium tuberculosis Induces Cytokine Production and Upregulates  the Function of Mouse Macrophages through TLR2. The Journal of Immunology, 2012, 188: 668–677

8.Xingui Tian, Xiaobo Su, Xiao Li. Protection against Enterovirus 71 with Neutralizing Epitope Incorporation within Adenovirus Type 3 Hexon. PLoS ONE. July 2012,Volume 7, Issue 7 , e41381.

9.Forster H.Physical and chemical properties of hydroxyethylstarch.Plasˉma Volume Expansion,1992,105-121.

10.Rubinstein P,Dobrila L,Rosenfield RE,et al.Processing and cryopˉreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution.Proc Natl Acad Sci,1995,92:10119-10122.

11.Denning-kendall P,Donaldson C,Nicol A,et al.Optimal processing of human
umbilical cord blood for clinical banking.Exp Hematol,1996,24:1394-1401.

12. 对外周血单个核细胞分离方法探讨. 韩亚萍 刘源 章莉莉 刘婷 李军 黄祖瑚 .中华现代临床医学杂志 2003年11月.1(9)

13.181-P: A rapid method to enrich specific lymphocyte populations (T cells, B cells, total lymphocytes) from whole blood. Karina L. McQueen, Jenna L. Warren, Allen C. Eaves, Terry E. Thomas.Human Immunology. Volume 68(1), Supplement: S106. 33rd Annual ASHI Meeting Abstracts October 2007

14. Collection, Storage, and Preparation of Human Blood Cells. Dagur PK, McCoy JP Jr. Curr Protoc Cytom. 2015 Jul 1;73:5.1.1-5.1.16

 

 

 

pluriselect2022年价格表

pluriselect细胞分离产品 – 用于生命科学研究的试剂盒、缓冲液和耗材。

生命科学研究用试剂盒、缓冲液和耗材。

细胞分离

细胞分离产品,用于正负细胞分离。直接从全血、水皮、PBMC等样品中分离。

细胞过滤器

细胞和样品过滤器几乎可以满足任何过滤需求。有19种网眼尺寸和多种管径可供选择。德国制造

密度梯度离心法

密度梯度培养基和离心管,用于分离PBMC(外周血单核细胞–含有淋巴细胞和单核细胞)、粒细胞、血小板或单核细胞,结果一致且可行。

媒体和缓冲区

细胞分离和细胞培养的缓冲液,如BSA-、EDTA-、PBS-原液、红细胞裂解缓冲液。

分子分析

消耗品

细胞培养、细胞分析和液体处理方面的所有消耗品。

ACCESSORIES

Unique mixing device, universal magnetic racks for most common tube sizes
pluriselect2022年价格表
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19-00400-10 CD4 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-00400-20 CD4 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
29-04500-10 CD45 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
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19-04500-20 CD45 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
29-04500-20 CD45 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
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43-50100-50 pluriStrainer 100µm, PET-mesh, 50 pcs – non-sterile  50 pcs – non-sterile 672 pluriSelect
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43-70200-03 ÜberStrainer, 200µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70010-03 ÜberStrainer, 10µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70015-03 ÜberStrainer, 15µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-10005-40 pluriStrainer Mini, 5µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10005-50 pluriStrainer Mini, 5µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10010-40 pluriStrainer Mini, 10µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10010-50 pluriStrainer Mini, 10µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10020-40 pluriStrainer Mini, 20µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10020-50 pluriStrainer Mini, 20µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10020-60 pluriStrainer Mini, 20µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-10040-40 pluriStrainer Mini, 40µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10040-50 pluriStrainer Mini, 40µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10040-60 pluriStrainer Mini, 40µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-10070-40 pluriStrainer Mini, 70µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10070-50 pluriStrainer Mini, 70µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10070-60 pluriStrainer Mini, 70µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-10070-70 pluriStrainer Mini, 70µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
41-50000-50 Connector Ring, 50 pcs – non-sterile  50 pcs – non-sterile 1440 pluriSelect
43-10100-40 pluriStrainer Mini, 100µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10100-60 pluriStrainer Mini, 100µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-50040-51 pluriStrainer 40µm, PET-mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
43-50100-51 pluriStrainer 100µm, PET-mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
43-50070-51 pluriStrainer 70µm, PET-mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
60-00050-11 1x RBC Lysis Buffer, 100ml  100ml 360 pluriSelect
43-10020-46 Bundle pluriStrainer® -Mini 20 µm 25 pcs and Flow Cytometry Tubes (PS) 25pcs, 25pcs per bag sterile  25pcs per bag sterile 960 pluriSelect
43-10020-66 Bundle pluriStrainer® -Mini 20 µm 100 pcs and Flow Cytometry Tubes (PS) 100pcs, 25pcs per bag sterile  25pcs per bag sterile 2520 pluriSelect
43-50001-13 pluriStrainer 1µm, 25 pcs, sterile  25 pcs 1392 pluriSelect
43-50005-13 pluriStrainer 5µm, 25 pcs, sterile  25 pcs 1392 pluriSelect
43-50050-03 PluriStrainer® 50 µm, 25 pcs. – sterile (Cell Strainer)  25 pcs. – sterile (Cell Strainer) 1392 pluriSelect
43-50150-03 pluriStrainer® 150 µm, 25 pcs. – sterile (Cell Strainer)  25 pcs. – sterile (Cell Strainer) 1392 pluriSelect
43-50060-50 pluriStrainer 60µm, 50 pcs – non-sterile  50 pcs – non-sterile 1824 pluriSelect
43-50050-50 PluriStrainer® 50 µm, 50 pcs. – non-sterile (Cell Strainer)  50 pcs. – non-sterile (Cell Strainer) 1824 pluriSelect
22-011b0-10 CD11b S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
43-50000-98 pluriStrainer Set 2, 24 pcs, sterile  24 pcs 1656 pluriSelect
70-50010-12 S-pluriBead® Maxi Reagent Kit kit 2424 pluriSelect
43-10005-60 pluriStrainer Mini, 5µm, 100pcs, 25pcs sterile in bag  5µm 2160 pluriSelect
29-F4800-20 F4/80 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
70-50010-22 M-pluriBead® Maxi Reagent Kit kit 2424 pluriSelect
29-00800-10 CD8 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
43-57040-03 pluriStrainer 40µm, Nylon-Mesh, 25 pcs, sterile  25 pcs 600 pluriSelect
43-57040-50 pluriStrainer 40µm, Nylon-Mesh, 50 pcs, non-sterile  50 pcs 672 pluriSelect
43-57040-51 pluriStrainer 40µm, Nylon-Mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
43-57070-03 pluriStrainer 70µm, Nylon-Mesh, 25 pcs, sterile  25 pcs 600 pluriSelect
43-57070-50 pluriStrainer 70µm, Nylon-Mesh, 50 pcs, non-sterile  50 pcs 672 pluriSelect
43-57070-51 pluriStrainer 70µm, Nylon-Mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
43-57100-03 pluriStrainer 100µm, Nylon-Mesh, 25 pcs, sterile  25 pcs 600 pluriSelect
43-57100-50 pluriStrainer 100µm, Nylon-Mesh, 50 pcs, non-sterile  50 pcs 672 pluriSelect
43-57100-51 pluriStrainer 100µm, Nylon-Mesh, 50 pcs, sterile  50 pcs 1008 pluriSelect
43-10040-76 Bundle pluriStrainer® -Mini 40 µm 500 pcs and Flow Cytometry Tubes (PS) 500pcs, 25pcs per bag sterile  25pcs per bag sterile 9024 pluriSelect
43-51000-03 pluriStrainer® 1000 µm, 25 pcs. – sterile (Cell Strainer)  25 pcs. – sterile (Cell Strainer) 1392 pluriSelect
29-F4800-10 F4/80 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
43-70005-03 ÜberStrainer, 5µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-10040-70 pluriStrainer Mini, 40µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-10100-50 pluriStrainer Mini, 100µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
01-00600-10 Buffy Coat Add-on Kit 1056 pluriSelect
05-00001-01 Centrifuge Tube 50 ml (sterile, 300 pcs.)  300 pcs. 1080 pluriSelect
05-00002-01 Centrifuge Tube 15 ml  (sterile, 500 pcs.)  500 pcs. 1608 pluriSelect
05-03040-01 Flow Cytometry Tube 5 ml (1000 pcs. , sterile)  sterile 2880 pluriSelect
19-00002-31 pluriSpin® Human PLT Depletion, 2ml  2ml 3360 pluriSelect
19-00200-10 CD2 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-00200-20 CD2 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-00300-20 CD3 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-00500-10 CD5 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-00500-20 CD5 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-00700-10 CD7 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-00700-20 CD7 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-00800-20 CD8 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-00800-10 CD8 S-pluriBead anti-hu, 2ml  2ml 1104 pluriSelect
19-01001-31 pluriSpin® Human Monocyte Enrichment, 2ml  2ml 3144 pluriSelect
19-00900-10 CD9 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-00900-20 CD9 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01001-32 pluriSpin® Human Monocyte Enrichment, 10ml  10ml 12480 pluriSelect
19-01002-31 pluriSpin® Human Monocyte Depletion, 2ml  2ml 1632 pluriSelect
19-011b0-10 CD11b S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-01002-32 pluriSpin® Human Monocyte Depletion, 10ml  10ml 7296 pluriSelect
19-011a0-10 CD11a S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-011a0-20 CD11a M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01400-20 CD14 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01400-10 CD14 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-011b0-20 CD11b M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01500-10 CD15 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-01500-20 CD15 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01600-10 CD16 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-01600-20 CD16 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-01900-10 CD19 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-01900-20 CD19 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-02001-32 pluriSpin® Human T Cell Enrichment, 10ml  10ml 12480 pluriSelect
19-02001-31 pluriSpin® Human T Cell Enrichment, 2ml  2ml 3144 pluriSelect
19-02002-31 pluriSpin® Human T Cell Depletion, 2ml  2ml 1632 pluriSelect
19-02002-32 pluriSpin® Human T Cell Depletion, 10ml  10ml 7296 pluriSelect
19-02100-10 CD21 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-02100-20 CD21 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-03001-31 pluriSpin® Human CD4+ T Cell Enrichment, 2ml  2ml 3144 pluriSelect
19-03001-32 pluriSpin® Human CD4? T Cell Enrichment, 10ml  10ml 12480 pluriSelect
19-03002-31 pluriSpin® Human CD4? Cell Depletion, 2ml  2ml 1632 pluriSelect
19-03002-32 pluriSpin® Human CD4? Cell Depletion, 10ml  10ml 7296 pluriSelect
19-03100-10 CD31 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-03100-20 CD31 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-04001-31 pluriSpin® Human CD8+ T Cell Enrichment, 2ml  2ml 3144 pluriSelect
19-04001-32 pluriSpin® Human CD8® T Cell Enrichment, 10ml  10ml 12480 pluriSelect
19-04002-31 pluriSpin® Human CD8+ Cell Depletion, 2ml  2ml 1632 pluriSelect
19-04002-32 pluriSpin® Human CD8? Cell Depletion, 10ml  10ml 7296 pluriSelect
19-04300-10 CD43 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-04300-20 CD43 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-04400-10 CD44 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-04400-20 CD44 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-04500-10 CD45 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-04700-10 CD47 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-04700-20 CD47 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-05001-31 pluriSpin® Human NK Cell Enrichment, 2ml  2ml 3144 pluriSelect
19-05001-32 pluriSpin® Human NK Cell Enrichment, 10ml  10ml 12480 pluriSelect
19-05002-31 pluriSpin® Human NK Cell Depletion, 2ml  2ml 1632 pluriSelect
19-05002-32 pluriSpin® Human NK Cell Depletion, 10ml  10ml 7296 pluriSelect
19-05500-10 CD55 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-05500-20 CD55 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-06001-32 pluriSpin® Human B Cell Enrichment, 10ml  10ml 12480 pluriSelect
19-06002-31 pluriSpin® Human CD19? Cell Depletion, 2ml  2ml 1632 pluriSelect
19-06002-32 pluriSpin® Human CD19? Cell Depletion, 10ml  10ml 7296 pluriSelect
19-08002-31 pluriSpin® Human PBMC Depletion, 2ml  2ml 1632 pluriSelect
19-08002-32 pluriSpin® Human PBMC Depletion, 10ml  10ml 7296 pluriSelect
19-09002-31 pluriSpin® Human CD45+ Cell Depletion, 2ml  2ml 1632 pluriSelect
19-09002-32 pluriSpin® Human CD45+ Cell Depletion, 10ml  10ml 7296 pluriSelect
19-09500-10 CD95 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-11700-10 CD117 S-pluriBead® anti-hu, 2ml  2ml 1440 pluriSelect
19-09500-20 CD95 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-11700-20 CD117 M-pluriBead® anti-hu, 2ml  2ml 2616 pluriSelect
19-13800-10 CD138 S-pluriBead® anti-hu, 2ml  2ml 1440 pluriSelect
19-13800-20 CD138 M-pluriBead® anti-hu, 2ml  2ml 2616 pluriSelect
19-19300-10 CD193 S-pluriBead® anti-hu, 2ml  2ml 1440 pluriSelect
19-19300-20 CD193 M-pluriBead® anti-hu, 2ml  2ml 2616 pluriSelect
19-235a0-10 CD235a S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-235a0-20 CD235a M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
19-27100-10 CD271 S-pluriBead® anti-hu, 2ml  2ml 1440 pluriSelect
19-27100-20 CD271 M-pluriBead® anti-hu, 2ml  2ml 2616 pluriSelect
19-32600-10 CD326 S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
19-32600-20 CD326 M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
22-00400-10 CD4 S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
22-00400-20 CD4 M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-008b0-10 CD8b S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
22-008b0-20 CD8b M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-011b0-20 CD11b M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-04400-10 CD44 S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
22-04400-20 CD44 M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-04500-10 CD45 S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
22-04500-20 CD45 M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-05500-10 CD55 S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
22-05500-20 CD55 M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
22-TCRAB-10 Anti TCRAB S-pluriBead® anti-rat, 2ml  2ml 1440 pluriSelect
23-01400-20 CD14 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
22-TCRAB-20 Anti TCRAB M-pluriBead® anti-rat, 2ml  2ml 2616 pluriSelect
23-011b0-10 CD11b S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-011b0-20 CD11b M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-01400-10 CD14 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-02100-10 CD21 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-02100-20 CD21 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-02900-10 CD29 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-02900-20 CD29 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-04400-10 CD44 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-04400-20 CD44 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-04700-10 CD47 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-04700-20 CD47 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-062L0-10 CD62L S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-062L0-20 CD62L M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-09000-10 CD90 S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-09000-20 CD90 M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-HLARP-10 HLARP S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-HLARP-20 HLARP M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
23-RBC00-10 RBC S-pluriBead® anti-porcine, 2ml  2ml 1440 pluriSelect
23-RBC00-20 RBC M-pluriBead® anti-porcine, 2ml  2ml 2616 pluriSelect
24-02900-10 CD29 S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-02900-20 CD29 M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-04400-10 CD44 S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-04100-20 CD41/61 M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-04100-10 CD41/61 S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-04500-20 CD45 M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-04400-20 CD44 M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-04700-10 CD47 S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-04700-20 CD47 M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-04500-10 CD45 S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-062L0-10 CD62L S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-062L0-20 CD62L M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
24-27100-10 CD271 S-pluriBead® anti-sheep, 2ml  2ml 1440 pluriSelect
24-HLARP-10 HLARP S-pluriBead® anti-sheep, 2ml  2ml 3600 pluriSelect
24-27100-20 CD271 M-pluriBead® anti-sheep, 2ml  2ml 2616 pluriSelect
25-00900-20 CD9 M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
24-HLARP-20 HLARP M-pluriBead® anti-sheep, 2ml  2ml 6540 pluriSelect
25-00900-10 CD9 S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-01400-10 CD14 S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-02100-10 CD21 S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-01400-20 CD14 M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
25-02100-20 CD21 M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
25-045R0-20 CD45R M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
25-045R0-10 CD45R S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-062L0-20 CD62L M-pluriBead® anti-bovine, 2ml  2ml 2616 pluriSelect
25-04700-20 CD47 M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
25-062L0-10 CD62L S-pluriBead® anti-bovine, 2ml  2ml 1440 pluriSelect
25-11700-10 CD117 S-pluriBead® anti-bovine, 2ml  2ml 1440 pluriSelect
25-11700-20 CD117 M-pluriBead® anti-bovine, 2ml  2ml 2616 pluriSelect
25-04700-10 CD47 S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-HLARP-10 HLARP S-pluriBead® anti-bovine, 2ml  2ml 3600 pluriSelect
25-HLARP-20 HLARP M-pluriBead® anti-bovine, 2ml  2ml 6540 pluriSelect
27-00400-20 CD4 M-pluriBead® anti-canine, 2ml  2ml 6540 pluriSelect
27-00400-10 CD4 S-pluriBead® anti-canine, 2ml  2ml 3600 pluriSelect
27-02900-20 CD29 M-pluriBead® anti-canine, 2ml  2ml 6540 pluriSelect
27-02900-10 CD29 S-pluriBead® anti-canine, 2ml  2ml 3600 pluriSelect
29-00300-10 CD3 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-00300-20 CD3 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-00400-10 CD4 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-00400-20 CD4 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-00800-20 CD8 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-011b0-10 CD11b S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-011b0-20 CD11b M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-011c0-10 CD11c S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-011c0-20 CD11c M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-01400-20 CD14 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-01400-10 CD14 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-01900-10 CD19 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-01900-20 CD19 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-03100-10 CD31 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-03100-20 CD31 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-32600-10 CD326 S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-32600-20 CD326 M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
29-Ly6G0-10 Ly6G S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
29-Ly6G0-20 Ly6G M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
31-GTaHS-10 universal S-pluriBead® anti-hamster, 2ml  2ml 1104 pluriSelect
31-GTaHS-20 universal M-pluriBead® anti-hamster, 2ml  2ml 1776 pluriSelect
31-GTaMS-10 universal S-pluriBead® anti-ms, 2ml  2ml 1104 pluriSelect
31-GTaMS-20 universal M-pluriBead® anti-ms, 2ml  2ml 1776 pluriSelect
31-GTaRB-10 universal S-pluriBead® anti-rabbit, 2ml  2ml 1104 pluriSelect
31-GTaRB-20 universal M-pluriBead® anti-rabbit, 2ml  2ml 1776 pluriSelect
31-GTaRT-10 universal S-pluriBead® anti-rat, 2ml  2ml 1104 pluriSelect
31-GTaRT-20 universal M-pluriBead® anti-rat, 2ml  2ml 1776 pluriSelect
31-RBaGT-20 universal M-pluriBead® anti-goat, 2ml  2ml 1776 pluriSelect
31-RBaHU-10 universal S-pluriBead® anti-hu, 2ml  2ml 1104 pluriSelect
31-RBaHU-20 universal M-pluriBead® anti-hu, 2ml  2ml 1776 pluriSelect
32-011a0-10 CD11a S-pluriBead® anti-horse, 2ml  2ml 3600 pluriSelect
32-04100-10 CD41/61 S-pluriBead® anti-horse, 2ml  2ml 3600 pluriSelect
32-04100-20 CD41/61 M-pluriBead® anti-horse, 2ml  2ml 6540 pluriSelect
33-01400-10 CD14 S-pluriBead® anti-rabbit, 2ml  2ml 3600 pluriSelect
32-011a0-20 CD11a M-pluriBead® anti-horse, 2ml  2ml 6540 pluriSelect
33-01400-20 CD14 M-pluriBead® anti-rabbit, 2ml  2ml 6540 pluriSelect
33-08100-20 CD81 M-pluriBead® anti-rabbit, 2ml  2ml 6540 pluriSelect
33-08100-10 CD81 S-pluriBead® anti-rabbit, 2ml  2ml 3600 pluriSelect
42-50000-50 Funnel, 50 pcs – non-sterile  50 pcs – non-sterile 1224 pluriSelect
43-10005-46 Bundle pluriStrainer® -Mini 5 µm 25 pcs and Flow Cytometry Tubes (PS) 25pcs, 25pcs per bag sterile  25pcs per bag sterile 960 pluriSelect
43-10005-66 Bundle pluriStrainer® -Mini 5 µm 100 pcs and Flow Cytometry Tubes (PS) 100pcs, 25pcs per bag sterile  25pcs per bag sterile 2520 pluriSelect
43-10005-56 Bundle pluriStrainer® -Mini 5 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10005-76 Bundle pluriStrainer® -Mini 5 µm 500 pcs and Flow Cytometry Tubes (PS) 500pcs, 25pcs per bag sterile  25pcs per bag sterile 9024 pluriSelect
43-10005-70 pluriStrainer Mini, 5µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-10010-46 Bundle pluriStrainer® -Mini 10 µm 25 pcs and Flow Cytometry Tubes (PS) 25pcs, 25pcs per bag sterile  25pcs per bag sterile 960 pluriSelect
43-10010-56 Bundle pluriStrainer® -Mini 10 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10010-60 pluriStrainer Mini, 10µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-10010-66 Bundle pluriStrainer® -Mini 10 µm 100 pcs and Flow Cytometry Tubes (PS) 100pcs, 25pcs per bag sterile  25pcs per bag sterile 2520 pluriSelect
43-10010-70 pluriStrainer Mini, 10µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-10010-76 Bundle pluriStrainer® -Mini 10 µm 500 pcs and Flow Cytometry Tubes (PS) 500pcs, 25pcs per bag sterile  25pcs per bag sterile 9024 pluriSelect
43-10020-56 Bundle pluriStrainer® -Mini 20 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10020-70 pluriStrainer Mini, 20µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-10020-76 Bundle pluriStrainer® -Mini 20 µm 500 pcs and Flow Cytometry Tubes (PS) 500pcs, 25pcs per bag sterile  25pcs per bag sterile 9024 pluriSelect
43-10040-46 Bundle pluriStrainer® -Mini 40 µm 25 pcs and Flow Cytometry Tubes (PS) 25pcs, 25pcs per bag sterile  25pcs per bag sterile 960 pluriSelect
43-10040-56 Bundle pluriStrainer® -Mini 40 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10040-66 Bundle pluriStrainer® -Mini 40 µm 100 pcs and Flow Cytometry Tubes (PS) 100pcs, 25pcs per bag sterile  25pcs per bag sterile 2520 pluriSelect
43-10070-46 Bundle pluriStrainer® -Mini 70 µm 25 pcs and Flow Cytometry Tubes (PS) 25pcs, 25pcs per bag sterile  25pcs per bag sterile 960 pluriSelect
43-10070-56 Bundle pluriStrainer® -Mini 70 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10070-66 Bundle pluriStrainer® -Mini 70 µm 100 pcs and Flow Cytometry Tubes (PS) 100pcs, 25pcs per bag sterile  25pcs per bag sterile 2520 pluriSelect
43-10070-76 Bundle pluriStrainer® -Mini 70 µm 500 pcs and Flow Cytometry Tubes (PS) 500pcs, 25pcs per bag sterile  25pcs per bag sterile 9024 pluriSelect
43-10100-46 Bundle pluriStrainer® Mini 100 µm and Flow Cytometry Tubes (25 pcs.) 25 pcs. 960 pluriSelect
43-10100-56 Bundle pluriStrainer® -Mini 100 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10100-66 Bundle pluriStrainer® Mini 100 µm and Flow Cytometry Tubes (100 pcs.) 100 pcs 2520 pluriSelect
43-10100-76 Bundle pluriStrainer® Mini 100 µm and Flow Cytometry Tubes (500 pcs.) 500 pcs 9024 pluriSelect
43-10100-70 pluriStrainer Mini, 100µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-50000-97 pluriStrainer Set 3, 24 pcs, sterile  24 pcs 1656 pluriSelect
43-50001-50 pluriStrainer 1µm, 50 pcs – non-sterile  50 pcs – non-sterile 1824 pluriSelect
43-50015-50 pluriStrainer 15µm, 50 pcs – non-sterile  50 pcs – non-sterile 1824 pluriSelect
43-50085-50 pluriStrainer 85µm, 50 pcs – non-sterile  50 pcs – non-sterile 1824 pluriSelect
43-50150-50 pluriStrainer® 150 µm, 50 pcs. – non-sterile (Cell Strainer)  50 pcs. – non-sterile (Cell Strainer) 1824 pluriSelect
43-50750-03 pluriStrainer® 750 µm, 25 pcs. – sterile (Cell Strainer)  25 pcs. – sterile (Cell Strainer) 1392 pluriSelect
43-50750-50 pluriStrainer® 750 µm, 50 pcs. – non-sterile (Cell Strainer)  50 pcs. – non-sterile (Cell Strainer) 1824 pluriSelect
43-50500-50 pluriStrainer 500µm, 50 pcs – non-sterile  50 pcs – non-sterile 1824 pluriSelect
43-51000-50 pluriStrainer® 1000 µm, 50 pcs. – non-sterile (Cell Strainer)  50 pcs. – non-sterile (Cell Strainer) 1824 pluriSelect
43-70000-97 ÜberStrainer Set 1 set 2784 pluriSelect
43-70000-98 ÜberStrainer Set 2 set 2784 pluriSelect
43-70000-99 ÜberStrainer Set 3 set 2784 pluriSelect
43-70001-03 ÜberStrainer, 1µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70020-03 ÜberStrainer, 20µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70030-03 ÜberStrainer, 30µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70040-03 ÜberStrainer, 40µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70060-03 ÜberStrainer, 60µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70070-03 ÜberStrainer, 70µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70085-03 ÜberStrainer, 85µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70100-03 ÜberStrainer, 100µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70300-03 ÜberStrainer, 300µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70400-03 ÜberStrainer, 400µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-70500-03 ÜberStrainer, 500µm, 10pcs, sterile  10pcs 2784 pluriSelect
43-71001-50 Syringe Strainer 1 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71005-50 Syringe Strainer 5 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71005-51 Syringe Strainer 5 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71010-50 Syringe Strainer 10 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71010-51 Syringe Strainer 10 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71015-50 Syringe Strainer 15 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71015-51 Syringe Strainer 15 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71020-50 Syringe Strainer 20 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71020-51 Syringe Strainer 20 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71030-50 Syringe Strainer 30 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71030-51 Syringe Strainer 30 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71040-50 Syringe Strainer 40 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71040-51 Syringe Strainer 40 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71050-50 Syringe Strainer 50 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71050-51 Syringe Strainer 50 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71060-50 Syringe Strainer 60 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71060-51 Syringe Strainer 60 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71070-50 Syringe Strainer 70 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71070-51 Syringe Strainer 70 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71085-50 Syringe Strainer 85 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71085-51 Syringe Strainer 85 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71100-50 Syringe Strainer 100 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71100-51 Syringe Strainer 100 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71200-50 Syringe Strainer 200 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71200-51 Syringe Strainer 200 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71300-50 Syringe Strainer 300 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71300-51 Syringe Strainer 300 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71400-50 Syringe Strainer 400 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71400-51 Syringe Strainer 400 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
43-71500-50 Syringe Strainer 500 µm (12 pcs., non-sterile)  12 pcs 1200 pluriSelect
43-71500-51 Syringe Strainer 500 µm (12 pcs., sterile)  12 pcs 1800 pluriSelect
44-00002-11 pluriMate – 2ml centrifuge tube, 100 pcs  100 pcs 1608 pluriSelect
44-00002-15 pluriMate – 2ml centrifuge tube, 500 pcs  500 pcs 7512 pluriSelect
44-00015-11 pluriMate – 15ml centrifuge tube, 100 pcs.  100 pcs. 1968 pluriSelect
44-00015-15 pluriMate – 15ml centrifuge tube, 500 pcs.  500 pcs. 9240 pluriSelect
44-00050-11 pluriMate – 50ml centrifuge tube, 100 pcs.  100 pcs. 2064 pluriSelect
44-00050-15 pluriMate – 50ml centrifuge tube, 500 pcs.  500 pcs. 9672 pluriSelect
50-01010-01 pluriMag Rack I device 3912 pluriSelect
50-01010-02 pluriMag Rack II device 3912 pluriSelect
60-00000-01 1x PBS, 100ml  100ml 72 pluriSelect
60-00000-10 1x PBS, 500ml  500ml 120 pluriSelect
60-00010-10 10x PBS, 100ml  100ml 108 pluriSelect
60-00010-11 10x PBS, 500ml  500ml 204 pluriSelect
60-00020-10 BSA stock solution, 50ml  50ml 432 pluriSelect
60-00020-11 BSA stock solution, 250ml  250ml 1776 pluriSelect
60-00020-12 BSA stock solution, 500ml  500ml 3072 pluriSelect
60-00030-10 EDTA Solution, 10ml  10ml 192 pluriSelect
60-00030-12 EDTA Solution, 100ml  100ml 864 pluriSelect
60-00046-12 Detachment Buffer (Buffer C & Buffer D) 552 pluriSelect
60-00050-12 1x RBC Lysis Buffer, 250ml  250ml 672 pluriSelect
60-00050-13 1x RBC Lysis Buffer, 500ml  500ml 1080 pluriSelect
60-00051-10 10x RBC Lysis Buffer, 100ml  100ml 1152 pluriSelect
60-00070-12 Buffer A (Stabilization Buffer), 10 ml  10 ml 336 pluriSelect
60-00060-12 Buffer B (Incubation Buffer), 10 ml  10 ml 552 pluriSelect
60-00091-10 Leuko Spin Medium, 100ml  100ml 372 pluriSelect
60-00091-11 Leuko Spin Medium, 250ml  250ml 768 pluriSelect
60-00080-11 10x Wash Buffer, 500ml  500ml 3072 pluriSelect
60-00091-12 Leuko Spin Medium, 500ml  500ml 1368 pluriSelect
60-00092-10 PBMC Spin Medium, 100ml  100ml 240 pluriSelect
60-00093-10 PBMC 24+ (Lympho 24+) Spin Medium, 100ml  100ml 211 pluriSelect
60-00092-11 PBMC Spin Medium, 250ml  250ml 480 pluriSelect
60-00093-11 PBMC 24+ (Lympho 24+) Spin Medium, 250ml  250ml 398 pluriSelect
60-00093-12 PBMC 24+ (Lympho 24+) Spin Medium, 500ml  500ml 696 pluriSelect
60-00094-10 PLT Spin Medium, 100ml  100ml 180 pluriSelect
60-00094-11 PLT Spin Medium, 250ml  250ml 336 pluriSelect
60-00094-12 PLT Spin Medium, 500ml  500ml 562 pluriSelect
60-00095-10 Monocytes Spin Medium, 100 100ml 197 pluriSelect
60-00095-12 Monocytes Spin Medium, 500 500ml 612 pluriSelect
60-00095-11 Monocytes Spin Medium, 250 250ml 372 pluriSelect
70-50010-21 M-pluriBead® Mini Reagent Kit kit 1392 pluriSelect
05-03040-03 Flow Cytometry Tube 5 ml (100 pcs. , sterile)  100 pcs. sterile) 1248 pluriSelect
43-10200-40 pluriStrainer Mini, 200µm, 25pcs sterile in bag  25pcs sterile in bag 768 pluriSelect
43-10200-46 Bundle pluriStrainer® Mini 200 µm and Flow Cytometry Tubes (25 pcs.) 25 pcs. 960 pluriSelect
43-10200-50 pluriStrainer Mini, 200µm, 50pcs, 25pcs sterile in bag  50pcs 1128 pluriSelect
43-10200-56 Bundle pluriStrainer® -Mini 200 µm 50 pcs and Flow Cytometry Tubes (PS) 50pcs, 25pcs per bag sterile  25pcs per bag sterile 1536 pluriSelect
43-10200-60 pluriStrainer Mini, 200µm, 100pcs, 25pcs sterile in bag  100pcs 2160 pluriSelect
43-10200-66 Bundle pluriStrainer® Mini 200 µm and Flow Cytometry Tubes (100 pcs.) 100 pcs 2520 pluriSelect
43-10200-70 pluriStrainer Mini, 200µm, 500pcs, 25pcs sterile in bag  500pcs 8400 pluriSelect
43-10200-76 Bundle pluriStrainer® Mini 200 µm and Flow Cytometry Tubes (500 pcs.) 500 pcs 9024 pluriSelect
44-09102-10 pluriMate – 2ml centrifugation tube, 50 pcs, pre-filled Leuko Spin  50 pcs 1320 pluriSelect
44-09102-11 pluriMate – 2ml centrifugation tube, 100 pcs, pre-filled Leuko Spin  100 pcs 2364 pluriSelect
44-09102-15 pluriMate – 2ml centrifugation tube, 500 pcs, pre-filled Leuko Spin  500 pcs 11496 pluriSelect
44-09202-10 pluriMate – 2ml centrifugation tube, 50 pcs, pre-filled PBMC Spin [old 44-91002-10]  50 pcs 1200 pluriSelect
44-09202-11 pluriMate – 2ml centrifugation tube, 100 pcs, pre-filled PBMC Spin [old 44-91002-11]  100 pcs 2184 pluriSelect
44-09202-15 pluriMate – 2ml centrifugation tube, 500 pcs, pre-filled PBMC Spin [old 44-91002-15]  500 pcs 10536 pluriSelect
44-09302-10 pluriMate – 2ml centrifugation tube, 50 pcs, pre-filled PBMC 24+ Spin  50 pcs 1200 pluriSelect
44-09302-11 pluriMate – 2ml centrifugation tube, 100 pcs, pre-filled PBMC 24+ Spin  100 pcs 2184 pluriSelect
44-09302-15 pluriMate – 2ml centrifugation tube, 500 pcs, pre-filled PBMC 24+ Spin  500 pcs 10536 pluriSelect
44-09115-10 pluriMate – 15ml centrifugation tube, 50 pcs, pre-filled Leuko Spin  50 pcs 2112 pluriSelect
44-09115-11 pluriMate – 15ml centrifugation tube, 100 pcs, pre-filled Leuko Spin  100 pcs 3552 pluriSelect
44-09115-15 pluriMate – 15ml centrifugation tube, 500 pcs, pre-filled Leuko Spin  500 pcs 17268 pluriSelect
44-09215-10 pluriMate – 15ml centrifugation tube, 50 pcs, pre-filled PBMC Spin [old 44-91015-10]  50 pcs 1752 pluriSelect
44-09215-11 pluriMate – 15ml centrifugation tube, 100 pcs, pre-filled PBMC Spin [old 44-91015-11]  100 pcs 3168 pluriSelect
44-09215-15 pluriMate – 15ml centrifugation tube, 500 pcs, pre-filled PBMC Spin [old 44-91015-15]  500 pcs 15336 pluriSelect
44-09315-10 pluriMate – 15ml centrifugation tube, 50 pcs, pre-filled PBMC 24+ Spin  50 pcs 1752 pluriSelect
44-09315-11 pluriMate – 15ml centrifugation tube, 100 pcs, pre-filled PBMC 24+ Spin  100 pcs 3168 pluriSelect
44-09315-15 pluriMate – 15ml centrifugation tube, 500 pcs, pre-filled PBMC 24+ Spin  500 pcs 15336 pluriSelect
44-09150-10 pluriMate – 50ml centrifugation tube, 50 pcs, pre-filled Leuko Spin  50 pcs 3960 pluriSelect
44-09150-11 pluriMate – 50ml centrifugation tube, 100 pcs, pre-filled Leuko Spin  100 pcs 6984 pluriSelect
44-09150-15 pluriMate – 50ml centrifugation tube, 500 pcs, pre-filled Leuko Spin  500 pcs 33096 pluriSelect
44-09250-10 pluriMate – 50ml centrifugation tube, 50 pcs, pre-filled PBMC Spin [old 44-91050-10]  50 pcs 3288 pluriSelect
44-09250-11 pluriMate – 50ml centrifugation tube, 100 pcs, pre-filled PBMC Spin [old 44-91050-11]  100 pcs 5928 pluriSelect
44-09250-15 pluriMate – 50ml centrifugation tube, 500 pcs, pre-filled PBMC Spin [old 44-91050-15]  500 pcs 28752 pluriSelect
44-09350-10 pluriMate – 50ml centrifugation tube, 50 pcs, pre-filled PBMC 24+ Spin  50 pcs 3288 pluriSelect
44-09350-11 pluriMate – 50ml centrifugation tube, 100 pcs, pre-filled PBMC 24+ Spin  100 pcs 5928 pluriSelect
44-09350-15 pluriMate – 50ml centrifugation tube, 500 pcs, pre-filled PBMC 24+ Spin  500 pcs 28752 pluriSelect
44-10015-10 pluriMate II – 15ml centrifuge tube, 50 pcs  50 pcs 1032 pluriSelect
44-10015-11 pluriMate II – 15ml centrifuge tube, 100 pcs  100 pcs 1824 pluriSelect
44-10015-15 pluriMate II – 15ml centrifuge tube, 500 pcs  500 pcs 8616 pluriSelect
44-10050-10 pluriMate II – 50ml centrifuge tube, 50 pcs  50 pcs 1104 pluriSelect
44-10050-11 pluriMate II – 50ml centrifuge tube, 100 pcs  100 pcs 1968 pluriSelect
44-10050-15 pluriMate II – 50ml centrifuge tube, 500 pcs  500 pcs 9192 pluriSelect
44-19115-10 pluriMate II – 15ml centrifugation tube, 50 pcs, pre-filled Leuko Spin  50 pcs 2040 pluriSelect
44-19115-11 pluriMate II – 15ml centrifugation tube, 100 pcs, pre-filled Leuko Spin  100 pcs 3408 pluriSelect
44-19215-11 pluriMate II – 15ml centrifugation tube, 100 pcs, pre-filled PBMC Spin  100 pcs 3024 pluriSelect
44-19115-15 pluriMate II – 15ml centrifugation tube, 500 pcs, pre-filled Leuko Spin  500 pcs 15816 pluriSelect
44-19215-10 pluriMate II – 15ml centrifugation tube, 50 pcs, pre-filled PBMC Spin  50 pcs 1680 pluriSelect
44-19315-10 pluriMate II – 15ml centrifugation tube, 50 pcs, pre-filled PBMC 24+ Spin  50 pcs 1680 pluriSelect
44-19150-11 pluriMate II – 50ml centrifugation tube, 100 pcs, pre-filled Leuko Spin  100 pcs 6840 pluriSelect
44-19150-15 pluriMate II – 50ml centrifugation tube, 500 pcs, pre-filled Leuko Spin  500 pcs 31632 pluriSelect
44-19150-10 pluriMate II – 50ml centrifugation tube, 50 pcs, pre-filled Leuko Spin  50 pcs 3816 pluriSelect
44-19215-15 pluriMate II – 15ml centrifugation tube, 500 pcs, pre-filled PBMC Spin  500 pcs 14808 pluriSelect
44-19315-11 pluriMate II – 15ml centrifugation tube, 100 pcs, pre-filled PBMC 24+ Spin  100 pcs 3024 pluriSelect
44-19315-15 pluriMate II – 15ml centrifugation tube, 500 pcs, pre-filled PBMC 24+ Spin  500 pcs 14808 pluriSelect
44-19350-11 pluriMate II – 50ml centrifugation tube, 100 pcs, pre-filled PBMC 24+ Spin  100 pcs 5736 pluriSelect
44-19250-10 pluriMate II – 50ml centrifugation tube, 50 pcs, pre-filled PBMC Spin  50 pcs 3144 pluriSelect
44-19250-11 pluriMate II – 50ml centrifugation tube, 100 pcs, pre-filled PBMC Spin  100 pcs 5736 pluriSelect
44-19250-15 pluriMate II – 50ml centrifugation tube, 500 pcs, pre-filled PBMC Spin  500 pcs 27312 pluriSelect
45-93006-10 TwinSpin; pre-filled with PBMC 24+, 50 pcs  50 pcs 4200 pluriSelect
46-10001-15 pluriStrainer Mini 1 µm (membrane, 5 pcs.), sterile  5 pcs 480 pluriSelect
45-94006-10 TwinSpin; pre-filled with PLT Spin, 50 pcs  50 pcs 4200 pluriSelect
45-92006-10 TwinSpin; pre-filled with PBMC Spin, 50 pcs  50 pcs 4200 pluriSelect
44-19350-10 pluriMate II – 50ml centrifugation tube, 50 pcs, pre-filled PBMC 24+ Spin  50 pcs 3144 pluriSelect
44-19350-15 pluriMate II – 50ml centrifugation tube, 500 pcs, pre-filled PBMC 24+ Spin  500 pcs 27312 pluriSelect
45-91006-10 TwinSpin; pre-filled with Leuko Spin, 50 pcs  50 pcs 4200 pluriSelect
46-10008-15 pluriStrainer Mini 8 µm (membrane, 5 pcs.), sterile  5 pcs 480 pluriSelect
46-10003-15 pluriStrainer Mini 3 µm (membrane, 5 pcs.), sterile  5 pcs 480 pluriSelect
46-10005-15 pluriStrainer Mini 5 µm (membrane, 5 pcs.), sterile  5 pcs 480 pluriSelect
60-01103-11 High Density Spin Medium (1.1), 250ml  250ml 912 pluriSelect
60-01103-12 High Density Spin Medium (1.1), 500ml  500ml 1632 pluriSelect
60-01063-11 Density Diluent Medium, 250ml  250ml 336 pluriSelect
60-01063-12 Density Diluent Medium, 500ml  500ml 516 pluriSelect
43-75100-40 Re-Strainer (In-Line-Strainer) 100 μm – Sterile, 25pcs  25pcs 2400 pluriSelect

transgenomic公司HPLC分离柱


transgenomic公司HPLC分离柱

简要描述:DNA Sep 分离柱是美国环球基因有限公司的技术, 这种柱子使用无孔多苯乙烯 – 二乙烯基苯( PS-DVB )共聚物微球体作为稳定基质,与含有 18 个碳原子的烷基长碳链相连,所以色谱柱的基质是疏水、电中性的,不会与核酸分子直接发生反应。

详细介绍

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HPLC分离柱
WAVE 系统关键技术 DNA 分离柱

DNA Sep 分离柱是美国环球基因有限公司的技术, 这种柱子使用无孔多苯乙烯 – 二乙烯基苯( PS-DVB )共聚物微球体作为稳定基质,与含有 18 个碳原子的烷基长碳链相连,所以色谱柱的基质是疏水、电中性的,不会与核酸分子直接发生反应。 三乙基铵醋酸盐( TEAA )是一种离子对试剂,离子对试剂既是疏水性的又带正电荷,既能与核酸主链上的磷酸基团的负电荷反应,同时 TEAA 的疏水基团又与固定相上碳 -18 链的疏水基团发生反应。这种离子对试剂起着连接核酸和柱基质之间的桥梁作用,使 DNA 片段吸附在固定相上面。然后,通过改变流动相中乙腈的浓度实现 DNA 片段的分离。

其 特点如下:

C 18 与 PS/DVB聚合物形成牢固的化学键结合

n 3微米微粒色谱柱填料 n 高效分离能力 n pH 值及温度范围: ( pH 2-14, Temp. 20- 95 0 C ) n 保证大于 6000 进样 n 具备DNA样品分析和纯化的能力 n 的分析稳定性 (>99%) n 色谱柱可再生性

下图: 分离柱表面的化学反应:TEAA分子与基质的微球体通过疏水键相连,而它的正电荷基团与带负电DNA分子相互作用。

订货号 名称 图片 性能特点
DNA-99-3510 DNASep Cartridge DNAsep柱应用于DNA分析
DNA-99-3710 DNASep HT DNA HT 分离柱应用于DNA快速分析(3分钟/样)
RNA-99-3810 RNASep Prep 7.8 x 50 mm 尺寸用于RNA分析或纯化
NUC-99-3550 OligoSep 4.6 x 50 mm 尺寸用于寡核苷算分析
NUC-99-3860 OligoSep Prep HC 7.8 x 50 mm 尺寸用于1000 nmoles量级寡核苷算纯化

淋巴细胞分离液

淋巴细胞分离液

产品编号 包装 价格
130010-L 60ml 86.00
130010-L1 120ml 125.00
130010-L2 200ml 190.00

产品简介  
    淋巴细胞分离液是一种根据细胞密度差异,借助离心产生的重力加速度,进行细胞的分离纯化的常用试剂。从外周血、骨髓、外周淋巴器官或各种组织分离出来的细胞是多种细胞的混合物,如要研究某种特定细胞的结构、功能或表面标志等特征,首先要分离纯化细胞。根据细胞的大小、密度、表面荷电、吸附能力或表面抗原的差异可实现对目的细胞的分离、纯化,常用的细胞分离与纯化方法有密度梯度沉降法、细胞电泳、贴附、亲和层析、花环形成、补体介导杀伤溶解以及流式细胞仪(FCM)和免疫磁珠分选等。密度梯度沉降法是根据细胞密度差异,借助细胞分离液和离心,进行细胞分离纯化的常用方法之一。细胞分离液则是一类能产生一定程度的密度梯度,高密度时粘度不高,浓度不同时渗透压变化不大,经洗涤去除后能用于体外培养,而对细胞没有毒性。zui常用的细胞分离液有Ficoll和Percoll。Ficoll是蔗糖的多聚体,呈中性,吸水性强,平均分子量为400,000,当密度为1.2g/ml仍未超出正常生理性渗透压,也不穿过生物膜。红细胞、粒细胞比重大,离心后沉于管底;淋巴细胞和单核细胞的比重小于或等于分层液比重,离心后漂浮于分层液的液面上,也可有少部分细胞悬浮在分层液中。吸取分层液液面的细胞,就可从外周血中分离到单个核细胞。  
    赛驰生物生产的淋巴细胞分离液(Lymphocytes Separation Medium)以Ficoll和泛影酸钠为主原材料,配制成密度为1.077的,无菌溶液。该产品适用于人淋巴细胞和大多数哺乳动物细胞的分离纯化,能除去红细胞和死细胞碎片。
包装清单  
    2-8℃避光保存。
使用说明

    1.在离心管中加入适量。
  2.取抗凝血(无溶血)与等量Hank’s液或平衡盐溶液充分混匀,用滴管沿管壁缓慢叠加于分层液面上,注意保持两液面界面清晰,水平离心2000rpm×20分钟。
  3.离心后,管内液体分为三层,上层为血浆和Hank’s液,下层主要为红细胞和粒细胞。中层为,在中上层液面处有一白色云雾层狭窄带,即为富含淋巴细胞和单核细胞的单个核细胞(PBMC)及血小板。
    4.吸去zui上层的血浆,收集血浆层和交界面的单个核细胞,尽量全部吸出PBMC,移入一新的离心管中;
    5.加入Hanks’液或无血清培养液,混匀后离心200×g 10分钟,弃去上清液(低速离心有利于去除细胞悬液中留存的血小板)。
    6.再用同样洗涤液洗涤细胞2次,每次离心500×g 10分钟,以洗去残留的。再用细胞培养液将细胞调配成适当浓度。通常,每毫升外周血可得1~2×106PBMC

产品编号
130010-L
130010-L1
130010-L2
60ml
120ml
200ml
说明书
1 份
保存条件

noninvasivetech粪便细胞分离系统说明书

noninvasivetech粪便细胞分离系统说明书

SCSR Fecal Cell Isolation System

 

研究人员和临床医生不需要侵入性活检,就可以研究与疾病相关的遗传标记和细胞表面标记,并监测患者的营养或疾病状况。SCSR粪便细胞分离系统通常每克粪便样本产生数百万个细胞,支持下游分析,包括细胞培养、核酸分析、细胞表面分析和基于患者自然史的无活检细胞存档。在过去的20年里,这项技术已经被用于从人类、大鼠、小鼠、牛、狗、猫、猴、山羊和绵羊样本中分离细胞。

        
DNA Extraction: Comparison of extraction from 200mg whole stool and extraction from 200mg equivalent of cells. 

DNA提取:从200mg粪便中提取和从200mg等量细胞中提取的比较。
比较室温下保持0小时(仅直接萃取)、3小时和24小时后的产率。
Compares yield after hold times of 0 hrs (direct extraction only), 3 hrs, and 24 hrs at room temperature. 


Fecal Cell Isolates  Left: Phase Contrast      Right: Rhodamine-labeled Phalloidin (Actin Stain)

       
Cultured Colonocyte  Left:  DIC     Right:  FITC-Labeled Anti-Human IgA

      
Fecal Cell Isolates: Left: Phase Contrast /Rhodamine-Phalloidin  Right: DIC/DAPI (DNA)

 
Fecal Cell Isolates
  Left: DIC     Middle: Rhodamine-Phalloidin     Right: DAPI

产品
SCSR公司™ 是可用的从粪便样本中分离细胞的试剂盒。我们的防腐介质允许样品在室温下运输最多5天。分离程序是一个简单的步骤梯度离心,然后在PBS中洗涤。细胞可以分析蛋白质、DNA和RNA,并且可以存档以备将来使用。试验表明,通过细胞的预分离,从粪便中提取的DNA得到了极大的改善。我们的离心方法优于珠或柱捕获,因为所有细胞类型都是分离的,不需要释放步骤[点击查看产品页面][点击查看技术信息]
我们还提供SCSR-T™, 一种用于生物样品的通用防腐剂和室温传输介质。它的配方是为了维持真核细胞的完整性和活力,以便进行下游分析。用SCSR-T保存的样品可在室温下运输,用于细胞分离、细胞培养、DNA和RNA提取、细胞内微生物分析、病毒与宿主基因组整合分析、药物基因组学、营养基因相互作用研究。SCSR-T公司™ 无毒无害[点击查看产品页面][点击查看技术信息]
研发
我们的实验室位于巴尔的摩和华盛顿之间的I-95走廊上,设备齐全,可进行流式细胞术、细胞分离、核酸提取、PCR、实时PCR、组织培养。


Adult progenitor cells isolated from stool samples 

 

 

 

 

 

 

 

 

IZON qEV高纯度外泌体分离柱说明书

 

IZON qEV高纯度外泌体分离柱说明书

产品型号: IZON

简要描述:qEV是一种根据尺寸排阻原理SEC (Size exclusion Chromatography)设计的一体 化外泌体分离/纯化技术。qEV有效地避免了传统离心方法在分离外泌体时,由于 超高离心力与蔗糖溶液高粘性造成的高丰度蛋白包裹及囊泡聚集的污染,也有效 避免了传统方法对于外泌体的膜结构的损伤,全面保留了外泌体的生物活性,可 用于外泌体功能实验。

 

qEV产品介绍

qEV是一种根据尺寸排阻原理SEC (Size exclusion Chromatography)设计的一体 化外泌体分离/纯化技术。qEV有效地避免了传统离心方法在分离外泌体时,由于 超高离心力与蔗糖溶液高粘性造成的高丰度蛋白包裹及囊泡聚集的污染,也有效 避免了传统方法对于外泌体的膜结构的损伤,全面保留了外泌体的生物活性,可 用于外泌体功能实验。

qEV分离原理

qEV进行外泌体分离时,将样品加入分离柱中,样品中小颗粒物质(脂类、蛋白等)会首先进入分离柱填充的排阻介 质孔隙中被固定住,而大分子物质(外泌体)则不能进入孔隙(如图)。

外泌体会在第7-11组分随缓冲液进入样品收集管,而高丰度蛋白等则会在第12组分开始被冲出(图左)。第7和8组分的外泌体富集度和纯度高,其含量可达第11组分及之后的分离组分的6000倍以上(图右)。

qEV产品优势

■方便、快速(仅需约15分钟);

qEVsingle   qEVoriginal    qEV2         qEV10

■高丰度蛋白去除率> 99%,高密度I旨蛋白(HDL)去除率>95% ;

■可保持外泌体、囊泡完整的结构和功能;

70nm+

■不同规格的qEV可分离特定粒径范围的外泌体;

■qEV尤其适用于细胞培养或动物注射等外泌体功能实验;

■适用于多种类型样品的外泌体提取,包括:血清、血浆、尿液、细胞

35nm+

上清、脑脊液、唾液、房水、宫腔灌洗液、腹水、胸水、胆汁、蜂王浆、 牛奶等。

qEVsingle

qEVoriginal
qEV2
qEV10
 

 

Axis-Shield介绍


Axis-Shield介绍

简要描述:挪威Axis-shield公司一直致力于分离技术的发展,该公司提供的一系列经碘化处理的密度梯度分离液不仅可以分离不同的细胞和细胞器而且可以分离纯化膜囊泡、病毒、蛋白质、核酸以及脂蛋白等物质。特别值得推荐的是,Axis-shield公司研制出的OptiprepTM和NycoprepTM Universal是一种全能型的梯度分离液详情见后。

详细介绍

产品咨询

 

AXIS-SHIELD 密度梯度分离液
现代细胞和分子生物学是以纯化的、有完整功能的细胞群、细胞器、和蛋白质等大分子物质为研究对象。几十年来,挪威Axis-shield公司一直致力于分离技术的发展,该公司提供的一系列经碘化处理的密度梯度分离液不仅可以分离不同的细胞和细胞器而且可以分离纯化膜囊泡、病毒、蛋白质、核酸以及脂蛋白等物质。特别值得推荐的是,Axis-shield公司研制出的OptiprepTMNycoprepTM Universal是一种全能型的梯度分离液详情见后。
Axis-shield公司的产品符合欧洲 cGMP 标准(相当美国FDA认证),它们具有无毒、低渗透度和分离纯化效果好的特点,因此在科研和临床检验中得到广泛的应用.
产品名称包装价格¥
1114544 LymphoprepTM 分离液(即用型) 1x250ml 690.00
1114545 LymphoprepTM 分离液(即用型) 4x250ml 1570.00
1114547 LymphoprepTM 分离液(即用型) 6x250ml 3150.00
1LymphoprepTM分离液
LymphoprepTM是目前国内外分离人外周血单个核细胞PBMC的常用方法即Ficoll-Isopaque法。
工作原理
PBMC与血液中的其他成分存在密度梯度,利用密度在1.077±0.001g/ml之间而且近于等渗的LymphoprepTM 溶液作密度梯度离心时,红细胞和粒细胞由于密度大,故沉于分层液底部。PBMC密度稍低于分层液,故位于分层液界面上,获得PBMCAxis-shield公司的LymphoprepTM 溶液含有多糖(polysacccharide,多糖能粘附在细胞表面,使红细胞和粒细胞聚集下沉,但它对淋巴细胞的活性和红细胞和粒细胞进一步分离有负面影响。
技术参数
泛影葡胺钠Sodium diatrizoate9.1% w/v; 多糖:5.7% w/v; 密度:1.077±0.001g/ml; 渗透性:280±15mOsm; 内毒素含量:<1.0EU/ml
2LymphoprepTM 分离管
LymphoprepTM 分离管是配合LymphoprepTM分离液用来分离人外周血单个核细胞PBMC的。该试管是无菌的,试管内壁中部有一个塑料过滤圈,LymphoprepTM分离液在过滤圈的下面。这种结构能防止血液和分离液搅在一起,而且红细胞和粒细胞能透过过滤圈沉到管底,取而代之,分离液位于过滤圈的上面。这样,有助于增强分离纯化的效果。
产品名称包装价格¥
1019818 LymphoprepTM 分离管(即用型) 18x10ml 1653.00
3NycoprepTM 1.077分离液(即用型)
工作原理
NycoprepTM 1.077 分离液主要是分离人外周血单个核细胞(PBMC),它和LymphoprepTM分离液的工作原理是相同的,密度也相同(1.077g/ml)。不同之处在于NycoprepTM 1.077分离液中非离子化的Nycodenz?代替泛影葡胺钠Sodium diatrizoate溶液。而且它不含有Ficollhe和多糖(polysacccharide),因此避免了LymphoprepTM分离液的缺点,具有更好的分离效果。
技术参数
Nycodenz14.1% w/v ; Nacl0.44% w/v; Tricine/NaoH, PH7.0: 5Mm; 
密度:1.077±0.001g/ml; 渗透性:295±15mOsm; 内毒素含量:<1.0EU/ml 
主要分离人外周血单个核细胞PBMC
产品名称包装价格¥
1114550 NycoprepTM 1.077 分离液(即用型) 4x250ml 1574.00
4NycoprepTM 1.068 分离液(即用型)
工作原理:
单个核细胞悬液并不是严格意义上的淋巴细胞,其中还含有少量的单核细胞及其它混杂细胞, NycoprepTM 1.068 分离液主要用于分离淋巴细胞和单核细胞。由于淋巴细胞(约1.070g/ml)和单核细胞(约1.065g/ml)的密度很接近,不容易分开。NycoprepTM 1.068 分离液利用本身的高渗透性,淋巴细胞比单核细胞更容易失水,在较短时间内使淋巴细胞的密度比单核细胞更大,在分界面上单核细胞位于淋巴细胞之上,从而达到分离目的。
技术参数
Nycodenz13.0% w/v; Nacl0.58% w/v; Tricine/NaoH, PH7.0: 5Mm; 
密度:1.068±0.001g/ml; 渗透性:335±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1002351 NycoprepTM 1.068 分离液(即用型) 1x100ml 369.00

5NycoprepTM 1.077Animal 分离液(即用型)
工作原理
通常,动物的淋巴细胞密度低于人的淋巴细胞密度,NycoprepTM 1.077Animal 分离液比NycoprepTM 1.077分离液具有较低的等渗透性这样尤其适于动物。目前,NycoprepTM 1.077Animal 分离液已在许多科研和临床实验中成功地应用于分离大鼠、小鼠、兔子血液中的单个核细胞。
技术参数
Nycodenz14.1% w/v; Nacl0.3% w/v; Tricine/NaoH, PH7.0: 5Mm;
密度:1.077±0.001g/ml; 渗透性:265±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1002380 NycoprepTM 1.077Animal 分离液(即用型) 1x100ml 289.00

6PolymorphprepTM 分离液即用型
工作原理:PolymorphprepTM 分离液高等渗透性可引起红细胞失水和收缩,增加了红细胞的浮力密度,从而使红细胞随着PolymorphprepTM 分离液(ρ=1.113g/ml)中的右旋糖苷溶液下沉。从而解决了红细胞浮力密度(ρ=1.09-1.11g/ml)和粒细胞密度十分相近而难分离的问题,使粒细胞和单核细胞在泛影葡胺钠界面分离开。
描述:主要用于分离多型核细胞PMN, 即粒细胞的分离。产品技术参数为泛影葡胺钠:
13.8% w/v; 右旋糖苷500: 8.0% w/v; 密度:1.113±0.001g/ml; 渗透性:460±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1001971 PolymorphprepTM 分离液即用型 1x100ml 294.00
7OptiprepTM 分离液(即用型)
工作原理
OptiprepTM 分离液是Axis-shield公司开发出的全能新型分离液,OptiprepTM 分离液的主要成分是odixanol,是一种高亲水性非离子的物质,这种主要成分决定了OptiprepTM 分离液是一种高密度、低粘度的溶液。而且,它的密度和渗透性成线形关系,在加入适当浓度的缓冲液或基本培养基,如蔗糖、HepesPRMI后,OptiprepTM 能形成连续的或不连续的等渗的密度梯度溶液,可将各种细胞、细胞器、脂蛋白分离开。经科研和临床实验证明,OptiprepTM 和常规的分离液FicollPercoll、蔗糖及氯化铯(CsCl)相比,OptiprepTM 分离液不仅分离纯化效果好,而且对各种细胞、细胞器的生命活性无任何影响。
描述:研制出的全能型分离液,主要成分为odixanol。可以用于分离细胞、亚细胞结构
和大分子物质。产品技术参数为Iodixanol: 60%w/v; 密度:1.320±0.001g/ml; 渗透
压:170±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1030061 OptiprepTM 分离液(即用型) 1x100ml 610.00
8NycoprepTM Universal分离液(即用型)
描述:主要成分是Iohexol Nycodenze是它的商标名,能将各种细胞、细胞核、细胞器、
脂蛋白等分离。产品技术参数为Nycodenz60% w/v; 密度:1.310±0.001g/ml; 渗透
性:580±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1106865 NycoprepTM Universal分离液(即用型) 1x300ml 1750.00
9Nycodenz 粉剂
描述:产品技术参数为Nycodenz 粉剂可溶于水溶液中浓度:80%w/v;密度:1.426g/ml
产品名称包装价格¥
1002424 Nycodenz 粉剂 1x500g 4450.00
10)泛影酸钠溶液Sodium diatrizoate solution 32.8%(即用型)
描述:产品技术参数为泛影酸钠: 32.8% w/v; 密度:1.200±0.002g/ml; 
渗透性:265±15mOsm; 内毒素含量:<1.0EU/ml
产品名称包装价格¥
1052878 泛影酸钠溶液Sodium diatrizoate solution 32.8%(即用型) 1x100ml 281.

www.axis-shield.com

POLYSCIENCES 21759-1说明书


 

美国POLYSCIENCES公司成立于1961年,主要生产和经营LIFE SCIENCES生命科学(胶体金,不含甲醛的甲醇等),病理学和显镜学 微球和粒子和磁珠各种单体和聚体等产品。polysciences公司是世界上较大较全的多聚化合物供应商,同时是的免疫学,组织化学试剂耗材供应商.

  POLYSCIENCES   21759-1现货

Nylon Wool Fiber T细胞分离尼龙毛柱

产品简介】

 尼龙毛柱是免疫学中一种用于分离T细胞的分离柱。忧郁在研究体内及体外的免疫系统时,分离淋巴细胞群是一个关键步骤。

描述

尼龙羊毛纤维可用于从外周血中分离白细胞并从B细胞中分离T细胞。粒细胞和B细胞粘附在纤维上,而T细胞和其他细胞则不粘。

准备使用,在10cc注射器中使用500mg预包装尼龙羊毛纤维。

10cc预装注射器Gamma处理25.0 – 40.0 kGy。

危害: 

无害使用正常预防措施

处理: 

运动正常护理

存储: 

在室温下储存

【详细说明】

 

尼龙毛柱

 

  尼龙毛柱是免疫学中一种用于分离T细胞的分离柱。忧郁在研究体内及体外的免疫系统时,分离淋巴细胞群是一个关键步骤。而市场上并没有针对B细胞的特异性抗血清,所以分离淋巴细胞中的T细胞并不是十分方便。T细胞尼龙毛分离法利用了尼龙毛对B细胞的亲和力,从而使T细胞在没有严重损伤的情况下达到的足够的纯度。该方法不像流式和磁珠费用昂贵,而且操作简便,所需要的条件也不高,是一种非常实用的方法。

 

  操作方法:

 

  1.秤取1.5g尼龙毛装入20-30ml玻璃容器或注射器内,填料的体积控制在15毫升左右。因为尼龙毛的松紧关系到T细胞的回收率,所以要特别注意。注射器下端配接5厘米左右长的带有弹簧夹的橡皮管。

 

  2. 加入大量的PBS平衡柱子后,用铝箔包裹,并置于适当的容器内,高压灭菌15分钟。

 

  (商品化尼龙毛柱以上步骤可以省略,经过PBS平衡后可以直接进行下列步骤。)

 

  3. 灭菌的尼龙毛柱垂直固定后放于超净台内,顶部以铝箔覆盖,避免污染。

 

  4. 注射器下端,橡皮管,弹簧夹用*消毒。打开弹簧夹调节流速在大约3-4ml/min。

 

  5.首先,加入3-4倍柱体的无血培养基平衡,之后加入相同体积的含有血清的培养基平衡(上述培养基都要预热),zui后等培养基液面接近柱填料液面时,关闭弹簧夹。

 

  6. 样本细胞悬浮液浓度控制在约5×108cells/ml,上样量一般是1/3-1/5柱体积。样品加入之后,再加入少量培养液,然后关闭弹簧夹。

 

  7.覆盖铝箔,垂直固定,置于消毒过的容器中,于培养箱内37℃孵育1小时。此过程中柱子必须要保持垂直状态。

 

  8. 从培养箱中拿出柱子,置于超净台内,除去铝箔。接上按照4.中的方法消毒的弹簧夹和橡皮管,加入适当体积经37℃预热的培养基,打开弹簧夹控制流速在3-4 mL/min,流出约5ml之后,流出的培养液基变得不透明,此时其中含有T细胞,收集这一部分培养基。

 

  9. 基本上何时结束收集取决于流出液体中细胞的浓度,实际上,收集的量达到一个柱体积时就可以停止收集,因为即使收集更多的量,回收率几乎不会增加。

 

  10.上述操作后,细胞的

 

  回收率为: 15~20 %

 

  T细胞含量: 85~95 %

 

  B细胞污染率: 1 5 %

 

  多数细胞被确定为T细胞。

 

(注)收集粘附于尼龙毛上的细胞时,将T细胞收集完毕后的尼龙毛再无菌操作的状态下取出,转移到适当的容器中,用镊子小心的将尼龙毛松开,粘附的细胞可以洗到培养基中。或者将注射器芯插入注射器内,通过压力使的粘附的细胞随着液体流出。

 

订货信息

货号

品名

包装

品牌

18369-50

尼龙毛

50g

POLYSCIENCES

18369-10

尼龙毛

10g

POLYSCIENCES

21759-1

尼龙毛柱 预装好

10支

POLYSCIENCES

 

 

 

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

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6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。

 

 

Cedarlane进口淋巴细胞分离液


Cedarlane进口淋巴细胞分离液

简要描述:Cedarlane淋巴细胞分离液可分离多个种属细胞来源,包括mouse, rat, rabbit, human and other mammalian等,所有产品为已经除菌的液体即用型,可用于从人或动物外周血、骨髓、脾等组织中分离获取活的淋巴细胞,同时高效去除红细胞及死细胞等

详细介绍

产品咨询

 来自加拿大Cedarlane公司,致力于向生命科学研究组织提供优质研究试剂,其淋巴细胞分离试剂以优质、高效、价廉赢得了用户的广泛认可。 

  Cedarlane淋巴细胞分离液可分离多个种属细胞来源,包括mouse, rat, rabbit, human and other mammalian等,所有产品为已经除菌的液体即用型,可用于从人或动物外周血、骨髓、脾等组织中分离获取活的淋巴细胞,同时高效去除红细胞及死细胞等。

 

*日期:2008.03.11-2008.04.30
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