信使RNA(mRNA)5 '帽状结构能够防止mRNA在细胞质中的降解,促进核糖体增长和蛋白的翻译。Trilink的帽类似物能够实现共转录加帽,这样就省去了加帽酶加帽操作。
CleanCap AG是TriLink公司用于5 '共转录加帽的产品,CleancapAG加帽可以形成Cap 1结构。CleancapAG的加帽效率高达98%。请注意:TriLink建议尽可能使用CleanCap AG,模板应该使用配套的AG启动子。Cleancap AG的加帽效率通常>90%。不像CleanCap AG,使用CleanCap GG(货号cat#N-7133或cat#N-7433)可能导致类似的ARCA转录效率和产量下降的现象。
上海金畔trilink现货产品:
货号 | 品名 | 规格 | 品牌 |
N-7003-10 | ARCA | 10umol | trilinkbiotech |
L-7702-100 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 100ug | trilinkbiotech |
N-2060-1 | 5-Hydroxymethyl-2-dCTP | 1umol | trilinkbiotech |
n-1013-1 | N6-Methyladenosine-5-TP | 1umol | trilinkbiotech |
N-4004-1 | 2,3-Dideoxythymidine-5-Triphosphate | 1umol | trilinkbiotech |
N-7003-5 | ARCA | 5umol | trilinkbiotech |
N-1087-1 | 5-Hydroxymethylcytidine-5-Triphosphate 5-Hydroxymethyl-CTP | 1umol | trilinkbiotech |
L-7606-1000 | CleanCap Cas9 mRNA | 1mg | trilinkbiotech |
L-7608-1000 | CleanCap beta gal mRNA | 1mg | trilinkbiotech |
N-2064-1 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
N-1084-1 | ON HOLD 5-Carboxy-Cytidine-5-Triphosphate 5-Carboxy-CTP 5-caCTP | 1umol | trilinkbiotech |
N-1010-10 | 2-Fluoro-2-deoxyuridine-5-Triphosphate | 10umol | trilinkbiotech |
N-1008-10 | 2-Fluoro-2-deoxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-1014-5 | 5-Methylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-1087-5 | 5-Hydroxymethylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-8006-1 | Cytidine-5-(1-Thiotriphosphate) | 1umol | trilinkbiotech |
N-8008-1 | 1-Thio-Uridine-5-Triphosphate | 1umol | trilinkbiotech |
L-7702-1000 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 1mg | trilinkbiotech |
N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-5002-050 | 生物标记dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
N-1087-10 | 5-Hydroxymethylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-1512-25 | Guanosine-5-Triphosphate | 25umol | trilinkbiotech |
N-1084-5 | 5-Carboxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-1085-1 | 5-Formylcytidine-5-Triphosphate 5-Formyl-CTP | 1umol | trilinkbiotech |
L-7606-100-100 ug | CleanCap Cas9 mRNA | 100ug | trilinkbiotech |
N-1066-1 | 8-Oxoguanosine-5-Triphosphate | 1umol | trilinkbiotech |
N-1066-5 | 8-Oxoguanosine-5-Triphosphate | 5umol | trilinkbiotech |
N-1065-1 | 5-Aminoallylcytidine-5-TP | 1umol | trilinkbiotech |
N-2063-5 | 5-Carboxy-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
L-7608-100 | CleanCap beta gal mRNA | 100µg | trilinkbiotech |
N-1085-10 | 5-Formylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-2020-1 | 2-Deoxyuridine-5-Triphosphate | 1umol | trilinkbiotech |
N-1084-10 | 5-Carboxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-5002-050 | 生物标记dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
N-2063-5 | 5-Carboxy-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-1087-10 | 5-Hydroxymethylcytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-1084-10 | 5-Carboxycytidine-5-Triphosphate | 10umol | trilinkbiotech |
N-1065-1 | 5-Aminoallylcytidine-5-TP | 1umol | trilinkbiotech |
N-1085-5 | 5-Formylcytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-1087-1 | 5-Hydroxymethylcytidine-5-Triphosphate 5-Hydroxymethyl-CTP | 1umol | trilinkbiotech |
N-1084-5 | 5-Carboxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-4004-5 | 2,3-Dideoxythymidine-5-Triphosphate | 5umol | trilinkbiotech |
L-7702-1000 | CleanCap Cyanine 5 FLuc mRNA (5moU) | 1mg | trilinkbiotech |
N-1010-1 | 2-Fluoro-2-deoxyuridine-5-TP | 1umol | trilinkbiotech |
N-5002-050 | 生物标记dCTP Biotin-16-Aminoallyl-2-deoxycytidine-5-TP | 50nmol | trilinkbiotech |
N-8008-1 | 1-Thio-Uridine-5-Triphosphate | 1umol | trilinkbiotech |
N-8002-5 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-8001-5 | 1-Thio-2-Deoxyadenosine-5-TP | 5umol | trilinkbiotech |
N-2064-10 | 5-Formyl-2-deoxycytidine-5-TP | 10umol | trilinkbiotech |
N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
N-8002-1 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
N-8001-1 | 1-Thio-2-Deoxyadenosine-5-Triphosphate | 1umol | trilinkbiotech |
N-2064-5 | 5-Formyl-2-deoxycytidine-5-Triphosphate | 5umol | trilinkbiotech |
n-1013-1 | N6-Methyladenosine-5-TP | 1umol | trilinkbiotech |
N-8002-1 | 1-Thio-2-Deoxycytidine-5-Triphosphate | 1umol | trilinkbiotech |
N-1008-1 | 2-Fluoro-2-deoxycytidine-5-TP | 1umol | trilinkbiotech |
N-2020-5 | 2-Deoxyuridine-5-Triphosphate | 5umol | trilinkbiotech |
Cleancap AU是mRNA表达过程中用于共转录加帽的产品,能够同步生产出具有天然结构Cap 1的mRNA。CleanCapAU是专为自复制RNA(saRNA)设计的,该saRNA基于正义RNA病毒,如委内瑞拉马脑炎病毒(VEEV)、塞姆利基森林病毒(SFV)和辛德比斯病毒(SIN)的基因组。这些正义RNA病毒基因组起始是一个5'-AU…Cap 1 mRNA。与传统的共转录转录加帽方法产生的Cap 0 mRNA相比,具有更高的体内活性。CleanCap可以与TriLink的修饰的NTP产品一起使用。