RealTimePrimers致力于为科研工作者提供价格低廉,经高度优化的实时定量PCR引物系列,结合高质量的模板和试剂,RealTimePrimers产品将为您呈现清晰、的PCR实验结果。现今,越来越多的研究者选择了RealTimePrimers产品。
实时定量PCR产物的产物定量有两种方式:使用已知浓度质粒DNA或对照基因的标准曲线来定量,和相对简单的相对定量法。两者都要保证扩增效率一致,但通常情况下,纯靶标产物的扩增效率要高于情况复杂的混合物样品,在实验中尤其要注意避免酚污染和(对RNA反转后定量)DNA污染等问题。
相对定量方法通过管家基因或对照基因来归一化靶标基因,是更常用的定量方式。在这一过程中选择合适的对照基因十分重要,通常需要一系列管家基因,以便综合或是找到稳定的基因来完成这一工作。
RealTimePrimers产品介绍:
1 信号通路PCR arrays (Pathway PCR arrays)
将信号通路上的基因集合形成arrays,一块微板上含88个靶标基因外加8个管家基因(housekeeping gene),大幅度降低了实验费用,提高了实验效率。
以人继发和先天免疫反应引物系列(Human Adaptive & Innate Immune Response Primer Library)为例,简单给予描述:
信号通路PCR arrays以96孔微板形式提供,每板含88个引物对和8个管家基因引物对,只需加入cDNA模板和SYBR green master mix即可实验。
2. 实时定量PCR引物
Realtimeprimers提供您所研究基因经证实的引物系列,并提供引物序列信息和详细的反应条件,可满足1000次PCR反应所需。
这类引物系列包括人、小鼠和大鼠三个物种。
3.提供管家基因引物序列信息,够1000次反应使用。通过比较多个看家基因表达,更好定量靶标基因的水平。
这类引物系列包括人、小鼠和大鼠三个物种。
以人housekeeping基因系列为例,包括基因:ACTB, B2M, GAPD, GUSB, HPRT1, PGK, PP1A, RPL13A, TBP和TFRC。
4. 基因家族(gene family)引物序列
将基因家族的成员汇总,提供已证实的引物序列,用于研究不同成员的表达差异,并提供引物的序列信息。
这类基因家族包括:
以人TNF基因家族为例,成员包括:TNF, LTA, LTB, TNFSF4, TNFSF5, TNFSF6, TNFSF7, TNFSF8, TNFSF9, TNFSF10, TNFSF11, TNFSF13, TNFSF13B, TNFSF14, TNFSF15, TNFSF18等
上海金畔realtimeprimers2022年价格表
货号 | 品名 | 规格 | 价格 | 品牌 |
AZ-1996 | AzuraQuant cDNA Synthesis Kit – 100 Reactions | 100 Reactions | 5015 | realtimeprimers |
AZ-1995 | AzuraQuant cDNA Synthesis Kit – 25 Reactions | 25 Reactions | 1445 | realtimeprimers |
AZ-2401 | AzuraView GreenFast qPCR Blue Mix Hi Rox – 200 Rxn | 200 Rxn | 935 | realtimeprimers |
AZ-2405 | AzuraView GreenFast qPCR Blue Mix Hi Rox – 500 Rxn | 500 Rxn | 2125 | realtimeprimers |
AZ-2420 | AzuraView GreenFast qPCR Blue Mix Hi Rox – 2000 Rxn | 2000 Rxn | 7650 | realtimeprimers |
AZ-2421 | AzuraView GreenFast qPCR Blue Mix Hi Rox – 10000 Rxn | 10000 Rxn | 37315 | realtimeprimers |
AZ-2301 | AzuraView GreenFast qPCR Blue Mix Lo Rox – 200 Rxn | 200 Rxn | 1870 | realtimeprimers |
AZ-2305 | AzuraView GreenFast qPCR Blue Mix Lo Rox – 500 Rxn | 500 Rxn | 4165 | realtimeprimers |
AZ-2320 | AzuraView GreenFast qPCR Blue Mix Lo Rox – 2000 Rxn | 2000 Rxn | 15215 | realtimeprimers |
AZ-2320 | AzuraView GreenFast qPCR Blue Mix Lo Rox – 10000 Rxn | 10000 Rxn | 37315 | realtimeprimers |
HACS-I | Human Actin Cytoskeleton Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HAIIR-I | Human Adaptive & Innate Immune Response Primer Library | ea | 6311.25 | realtimeprimers |
HATJ-I | Human Adherin – Tight Junction Primer Library | ea | 6311.25 | realtimeprimers |
HADG-I | Human Adipogenesis Primer Library | ea | 6311.25 | realtimeprimers |
HAZD-I | Human Alzheimer's Disease Primer Library | ea | 6311.25 | realtimeprimers |
HAMM-I | Human Amino Acid Metabolism Primer Library | ea | 7586.25 | realtimeprimers |
HAND-I | Human Androgen Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HAPL-I | Human Angiogenesis Primer Library | ea | 5036.25 | realtimeprimers |
HAPP-I | Human Antigen Processing and Presentation Primer Library | ea | 6311.25 | realtimeprimers |
HPA-I | Human Apoptosis Primer Library | ea | 5036.25 | realtimeprimers |
HATH-I | Human Atherosclerosis Primer Library | ea | 6311.25 | realtimeprimers |
HATPL-I | Human Autophagy Primer Library | ea | 5036.25 | realtimeprimers |
HBCR-I | Human B-Cell Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HBDNF-I | Human BDNF Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HBLCP-I | Human Bladder Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HBMP-I | Human BMP Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HBRC-I | Human Brain Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HBRCA-I | Human Breast Cancer Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HCAR-I | Human Calcium Regulation Pathway | ea | 6311.25 | realtimeprimers |
HCAMP-I | Human cAMP Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HCDG-I | Human Cancer Driver Gene I Primer Library | ea | 7586.25 | realtimeprimers |
HCDG-II | Human Cancer Driver Gene II Primer Library | ea | 7586.25 | realtimeprimers |
HCAN-I | Human Cancer Pathway Primer Library | ea | 6311.25 | realtimeprimers |
HCAD-I | Human Cell Adhesion Primer Library | ea | 7586.25 | realtimeprimers |
HCC-I | Human Cell Cycle Primer Library | ea | 5036.25 | realtimeprimers |
HCHR-I | Human Chromatin Regulation Primer Library | ea | 7586.25 | realtimeprimers |
HCCP-I | Human Colon Cancer Primer Library I | ea | 6311.25 | realtimeprimers |
HCCP-II | Human Colon Cancer Primer Library II | ea | 6311.25 | realtimeprimers |
HCREB-I | Human CREB Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HCCR-I | Human Cytokine and Chemokine Receptor Primer Library | ea | 7586.25 | realtimeprimers |
HCA-I | Human Cytokine Primer Library I | ea | 5036.25 | realtimeprimers |
HCA-II | Human Cytokine Primer Library II | ea | 5036.25 | realtimeprimers |
HSCK-I | Human Cytoskeleton Primer Library | ea | 7586.25 | realtimeprimers |
HDRL-I | Human DNA Repair Primer Library I | ea | 6311.25 | realtimeprimers |
HDRL-II | Human DNA Repair Primer Library II | ea | 6311.25 | realtimeprimers |
HDTX-I | Human Drug Detoxication I | ea | 6311.25 | realtimeprimers |
HDTX-II | Human Drug Detoxication II | ea | 7586.25 | realtimeprimers |
HDTP-I | Human Drug Transporter I Primer Library | ea | 6311.25 | realtimeprimers |
HEGFR-I | Human EGFR Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HEND-I | Human Endothelial Cell Primer Library | ea | 6311.25 | realtimeprimers |
HEMT-I | Human Epithelial Mesenchymal Transition Primer Library | ea | 6311.25 | realtimeprimers |
HERBB-I | Human ERBB Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HESR-I | Human Estrogen Signaling Primer Library | ea | 7586.25 | realtimeprimers |
HFLM-I | Human Fatty Acid and Lipid Metabolism Primer Library | ea | 6311.25 | realtimeprimers |
HFGF-I | Human FGF Receptor Signaling Primer Library | ea | 7586.25 | realtimeprimers |
HFCA-I | Human Focal Adhesion Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HGCPL-I | Human Gastric Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HGDNF-I | Human GDNF Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HGLM-I | Human Glucose Metabolism Primer Library | ea | 5036.25 | realtimeprimers |
HGPCR-I | Human G-Protein Coupled Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HHSP-I | Human Heat Shock Protein Primer Library | ea | 8861.25 | realtimeprimers |
HHOG-I | Human Hedgehog Signaling Primer Library | ea | 7586.25 | realtimeprimers |
HHPO-I | Human Hippo Signaling Primer Pathway | ea | 6311.25 | realtimeprimers |
HHOX-I | Human Homeobox Gene Primer Library | ea | 7586.25 | realtimeprimers |
HHYP-I | Human Hypoxia Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HIL17-I | Human IL17 Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HINS-I | Human Insulin Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HINT-I | Human Integrin Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HIFN-I | Human Interferon Type I Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HIFN-II | Human Interferon Type II Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HJAK-I | Human JAK/STAT Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HKIN-X | Human Kinase Library (I, II, III and IV) | ea | 17786.25 | realtimeprimers |
HKIN-I | Human Kinase Library I | ea | 5036.25 | realtimeprimers |
HKIN-II | Human Kinase Library II | ea | 5036.25 | realtimeprimers |
HKIN-III | Human Kinase Library III | ea | 5036.25 | realtimeprimers |
HKIN-IV | Human Kinase Library IV | ea | 5036.25 | realtimeprimers |
HLGI-I | Human Ligand Gated Ion Channels Primer Library | ea | 8861.25 | realtimeprimers |
HLCPL-I | Human Liver Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HLUCPL-I | Human Lung Cancer PCR Primer Library | ea | 6311.25 | realtimeprimers |
HMAP-I | Human MAP Kinase Signaling Library | ea | 6311.25 | realtimeprimers |
HMEL-I | Human Melanoma Primer Library | ea | 6311.25 | realtimeprimers |
HMDSC-I | Human Myeloid Derived Suppressor Cell Primer Library | ea | 6311.25 | realtimeprimers |
HNCR-I | Human Necroptosis Primer Library | ea | 6311.25 | realtimeprimers |
HNTS-I | Human Neurotransmitter Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HNFKB-I | Human NFKappaB Primer Library | ea | 5036.25 | realtimeprimers |
HNOS-I | Human Nitric Oxide Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HNKT-I | Human NK Cell Mediated Toxicity Primer Library | ea | 6311.25 | realtimeprimers |
HNOT-I | Human Notch Signaling Pathway | ea | 6311.25 | realtimeprimers |
HCirc-Onc-I | Human Oncogene circRNA Library | ea | 11411.25 | realtimeprimers |
HONC-I | Human Oncogene Primer Library | ea | 6311.25 | realtimeprimers |
HOST-I | Human Osteogenesis Primer Library | ea | 6311.25 | realtimeprimers |
HOVCL-I | Human Ovarian Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HOSL-I | Human Oxidative Stress Primer Library | ea | 5036.25 | realtimeprimers |
HTPS-I | Human p53 Signaling Primer Library | ea | 5036.25 | realtimeprimers |
HPANC-I | Human Pancreatic Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HPKD-I | Human Parkinson's Disease Primer Library | ea | 6311.25 | realtimeprimers |
HPDGF-I | Human PDGF Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HAKT-I | Human PI3K-AKT Signaling Primer Library | ea | 5036.25 | realtimeprimers |
HPRC-I | Human Prostate Cancer Primer Library | ea | 6311.25 | realtimeprimers |
HRAS-I | Human Ras Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HRBG-I | Human Retinoblastoma Gene Primer Library | ea | 6311.25 | realtimeprimers |
HRGTP-I | Human Rho GTPase-Signaling Primer Library | ea | 7586.25 | realtimeprimers |
HSCH-I | Human Schwann Cell PCR Primer Library | ea | 6311.25 | realtimeprimers |
HSTL-I | Human Signal Transduction Primer Library | ea | 5036.25 | realtimeprimers |
HSMAD-I | Human SMAD Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HSTAT-I | Human Statin Signaling Library | ea | 6311.25 | realtimeprimers |
HSCL-I | Human Stem Cell Primer Library | ea | 5036.25 | realtimeprimers |
HTAIT-I | Human T-Cell Anergy/Immune Tolerance Primer Library | ea | 6311.25 | realtimeprimers |
HTDD-I | Human T-Cell Development and Differentiation Primer Library | ea | 6311.25 | realtimeprimers |
HTCR-I | Human T-Cell Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HTGFB-I | Human TGF Beta Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HTHD-I | Human Th1 and Th2 Cell Differentiation Primer Library | ea | 6311.25 | realtimeprimers |
HTNF-I | Human TNF Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HTLR-I | Human Toll-like Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HTIMU-I | Human Tumor Immunity Primer Library | ea | 6311.25 | realtimeprimers |
HTIM-I | Human Tumor Invasion/Metastasis Primer Library | ea | 5036.25 | realtimeprimers |
HVEGF-I | Human VEGF Signaling Primer Library | ea | 6311.25 | realtimeprimers |
HWNT-I | Human WNT Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MATJ-I | Mouse Adherin – Tight Junction Primer Library | ea | 6311.25 | realtimeprimers |
MADG-I | Mouse Adipogenesis Primer Library | ea | 6311.25 | realtimeprimers |
MAPL-I | Mouse Angiogenesis Primer Library | ea | 5036.25 | realtimeprimers |
MPA-I | Mouse Apoptosis Primer Library | ea | 5036.25 | realtimeprimers |
MATPL-I | Mouse Autophagy Primer Library | ea | 5036.25 | realtimeprimers |
MBDNF-I | Mouse Bdnf Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MBMP-I | Mouse BMP Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MCC-I | Mouse Cell Cycle Primer Library | ea | 5036.25 | realtimeprimers |
MCREB-I | Mouse CREB Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MCCR-I | Mouse Cytokine and Chemokine Receptor Primer Library | ea | 7586.25 | realtimeprimers |
MCA-I | Mouse Cytokine Primer Library I | ea | 5036.25 | realtimeprimers |
MCA-II | Mouse Cytokine Primer Library II | ea | 5036.25 | realtimeprimers |
MDDS-I | Mouse DNA Damage Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MDRL-I | Mouse DNA Repair Primer Library I | ea | 7586.25 | realtimeprimers |
MDRL-II | Mouse DNA Repair Primer Library II | ea | 7586.25 | realtimeprimers |
MEGFR-I | Mouse EGFR Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MEMT-I | Mouse Epithelial-Mesenchymal Transition Primer Library | ea | 6311.25 | realtimeprimers |
MESR-I | Mouse Estrogen Receptor Signaling Library | ea | 7586.25 | realtimeprimers |
MFTL-I | Mouse Fatty Liver Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MGDNF-I | Mouse Gdnf Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MGLM-I | Mouse Glucose Metabolism Primer Library | ea | 6311.25 | realtimeprimers |
MHPO-I | Mouse Hippo Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MHYP-I | Mouse Hypoxia Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MINT-I | Mouse Integrin Signaling Primer Library | ea | 8861.25 | realtimeprimers |
MJAK-I | Mouse Jak/Stat Signaling Primer Library | ea | 7586.25 | realtimeprimers |
MMAP-I | Mouse MAP Kinase Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MMDSC-I | Mouse Myeloid Derived Suppressor Cell Primer Library | ea | 6311.25 | realtimeprimers |
MNTS-I | Mouse Neurotransmitter Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MNFKB-I | Mouse NFKappaB Primer Library | ea | 5036.25 | realtimeprimers |
MNOT-I | Mouse Notch Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MONC-I | Mouse Oncogene Primer Library | ea | 6311.25 | realtimeprimers |
MOSL-I | Mouse Oxidative Stress Primer Library | ea | 6311.25 | realtimeprimers |
MTPS-I | Mouse p53 Signaling Primer Library | ea | 5036.25 | realtimeprimers |
MAKT-I | Mouse PI3K-AKT Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MRAS-I | Mouse Ras Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MTCR-I | Mouse T-Cell Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MTGFB-I | Mouse TGF Beta Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MTLR-I | Mouse Toll-like Receptor Signaling Primer Library | ea | 6311.25 | realtimeprimers |
MTIMU-I | Mouse Tumor Immunity Primer Library | ea | 6311.25 | realtimeprimers |
MTIM-I | Mouse Tumor Invasion/Metastasis Primer Library | ea | 5036.25 | realtimeprimers |
MWNT-I | Mouse WNT Signaling Primer Library | ea | 6311.25 | realtimeprimers |
RATPL-I | Rat Autophagy Primer Library | ea | 7586.25 | realtimeprimers |
RBDNF-I | Rat Bdnf Signaling Primer Library | ea | 7586.25 | realtimeprimers |
RCA-I | Rat Cytokine Primer Library I | ea | 6311.25 | realtimeprimers |
RCA-II | Rat Cytokine Primer Library II | ea | 6311.25 | realtimeprimers |
RGDNF-I | Rat Gdnf Signaling Primer Library | ea | 7586.25 | realtimeprimers |
RGLM-I | Rat Glucose Metabolism Primer Library | ea | 6311.25 | realtimeprimers |
RINT-I | Rat Integrin Signaling Primer Library | ea | 10136.25 | realtimeprimers |
RNTS-I | Rat Neurotransmitter Signaling Primer Library | ea | 7586.25 | realtimeprimers |
RNFKB-I | Rat NFKappaB Primer Library | ea | 7586.25 | realtimeprimers |
ROSL-I | Rat Oxidative Stress Primer Library | ea | 6311.25 | realtimeprimers |
RUBM-I | Rat Ubiquitin Metabolism Primer Library | ea | 7586.25 | realtimeprimers |