标签归档：异构酶

topogen代理

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topogen代理-上海金畔

TopoGEN是一家研究诊断公司，为从事DNA拓扑异构酶和DNA修复的研究人员提供创新的产品和服务。从事基于机制的抗癌、抗病毒和抗菌药物发现的科学家会发现其产品在研发中特别有用。他们的药物筛选试剂盒可用于明确鉴定拓扑异构酶靶向分子，用于癌症化疗和抗菌应用。TopoGEN还销售检测和定量原核（DNA Gyrase，拓扑异构酶IV）和真核拓扑异构酶（拓扑异构酶I，IIa，IIb，III）的检测试剂盒。TopoGEN还提供全系列的 DNA 底物和 DNA 切割靶标、拓扑异构酶抑制剂、拓扑异构酶抗体和试剂盒补充试剂。DNA修复的相关系列产品也是他们产品的关键部分。

TopoGEN生产和销售研究高质量试剂，并了解出版物和专li所需的证据标准。TopoGEN的技术支持人员致力于为您（研究人员）提供完成研究目标所需的培训和敏锐度。

topogen常卖产品：

货号 品名 规格 品牌
DR3000 HRHeLa-Sce HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR3000 HRHeLa-Sce2 HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR3000 HRHeLa-Sce3 HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR3000 HRHeLa-CPR HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA) 1Kit topogen
DR3000 HRHeLa-CPR2 HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) 1Kit topogen
DR3000 HRHeLa-CPR3 HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) 1Kit topogen
DR3000 HRCN1.4-Sce HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR3000 HRCN1.4-Sce2 HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR3000 HRCN1.4-Sce3 HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR3000 HRSH-Sce HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR3000 HRSH-Sce2 HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR3000 HRSH-Sce3 HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR3000 HR3T3-Sce HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR3000 HR3T3-Sce2 HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR3000 HR3T3-Sce3 HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR3000 Custom-Sce Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (50ug DNA) 1Kit topogen
DR3000 Custom-Sce2 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (100ug DNA) 1Kit topogen
DR3000 Custom-Sce3 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (250ug DNA) 1Kit topogen
DR3000A Custom-Sce Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (50ug DNA) 1Kit topogen
DR3000A Custom-Sce2 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (100ug DNA) 1Kit topogen
DR3000A Custom-Sce3 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (250ug DNA) 1Kit topogen
DR5001 HNHela-Sce NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR5001 HNHela-Sce2 NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR5001 HNHela-Sce3 NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR5000 HNSH-Sce NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA) 1Kit topogen
DR5000 HNSH-Sce2 NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA) 1Kit topogen
DR5000 HNSH-Sce3 NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA) 1Kit topogen
DR5000 HNCN1.4-Sce NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA) 1Kit topogen
DR5000 HNCN1.4-Sce2 NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA) 1Kit topogen
DR5000 HNCN1.4-Sce3 NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA) 1Kit topogen
DR5000 HN3T3-Sce NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA) 1Kit topogen
DR5000 HN3T3-Sce2 NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA) 1Kit topogen
DR5000 HN3T3-Sce3 NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA) 1Kit topogen
DR5000 Custom-Sce Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (50ug DNA) 1Kit topogen
DR5000 Custom-Sce2 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (100ug DNA) 1Kit topogen
DR5000 Custom-Sce3 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (250ug DNA) 1Kit topogen
DR5000A Custom-Sce Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (50ug DNA) 1Kit topogen
DR5000A Custom-Sce2 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (100ug DNA) 1Kit topogen
DR5000A Custom-Sce3 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (250ug DNA) 1Kit topogen
DR3050-1 HR Pooled Cell analysis:  50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP 1Kit topogen
DR3050-2 HeLa Cell RepHR Pooled Cell analysis:  250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP 1Kit topogen
DR3050-3 HR Pooled Cell analysis:  500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP 1Kit topogen
DR3050-4 HR Pooled Cell analysis:  1000ug DR-HRGFP, 1000ug pSce1, 25ug e-GFP 1Kit topogen
DRT5050-1 Detailed protocols with 50ug of cNHEJ plasmid, 50ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRT5050-2 Detailed protocols with 250ug of cNHEJ plasmid, 250ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRT5050-3 Detailed protocols with 500ug of cNHEJ plasmid, 500ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRT5050-4 Detailed protocols with 1000ug of cNHEJ plasmid, 1000ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRA5050-1 Detailed protocols with 50ug of Alt-NHEJ plasmid, 50ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRA5050-2 Detailed protocols with 250ug of Alt-NHEJ plasmid, 250ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRA5050-3 Detailed protocols with 500ug of Alt-NHEJ plasmid, 500ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
DRA5050-4 Detailed protocols with 1000ug of Alt-NHEJ plasmid, 1000ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 1Kit topogen
TG1050-1 Topoisomerase Type IIa Nuclear Extraction/Assay Kit 100assays topogen
TG1001-1 Topoisomerase II Assay Kit (20ug kDNA) 100assays topogen














TOPOGEN

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TOPOGEN

简要描述：TopoGEN 公司是一家专门为拓扑酶研究提供试剂和试剂盒。试剂盒可以有效地分析拓扑异构酶 I ， II 和旋转酶，分析细胞内的拓扑抑制以及描绘拓扑异构酶抑制剂的特性。另外公司还提供 DNa c底物，拓扑抑制剂和抗体等。

详细介绍

产品咨询

 


TopoGEN 公司是一家专门为拓扑酶研究提供试剂和试剂盒。试剂盒可以有效地分析拓扑异构酶 I ， II 和旋转酶，分析细胞内的拓扑抑制以及描绘拓扑异构酶抑制剂的特性。另外公司还提供 DNa c底物，拓扑抑制剂和抗体等。
  

topogen TG1004-1 Tdp1 Assay Kit 100 Assays 4482
topogen TG1004-2 Tdp1 Assay Kit 250 Assays 6282
topogen TG1007 Topoisomerase IV Drug Screening Kit 100 Assays 4302
topogen TG1003 Gyrase Assay Kit 100 Assays 5382
topogen TG1001-1 Human Topoisomerase II Assay Kit 100 Assays 4842
topogen TG1001-2 Human Topoisomerase II Assay Kit 250 Assays 6822
topogen TG1009-1 Topoisomerase II Drug Screening Kit 100 Assays 4842
topogen TG1009-2 Topoisomerase II Drug Screening Kit 250 Assays 6822
topogen TG1015-1 Topoisomerase I Assay Kit 100 Assays 4482
topogen TG1015-2 Topoisomerase I Assay Kit 250 Assays 6282
topogen TG1011-1 SDS-K+ Precipitation Kit （in vivo） 100 Assays 5382
topogen TG1011-2 SDS-K+ Precipitation Kit （in vivo） 250 Assays 6822
topogen TG1018-1 Topoisomerase I Drug Screening Kit 100 Assays 4842
topogen TG1018-2 Topoisomerase I Drug Screening Kit 250 Assays 6642
topogen TG1021 Topoisomerase I In Vivo Link Kit 200 Assays 7182
topogen TG1022 Topoisomerase II In Vivo Link Kit 200 Assays 8442
topogen TG1010-1 SDS-K+ Precipitation Kit （in vitro） 100 Assays 5382
topogen TG1010-2 SDS-K+ Precipitation Kit （in vitro） 250 Assays 7182
topogen TG1025 DNA Intercalator/Unwinding Kit 50 Experiments 6282
topogen TG1030 Topoisomerase II Immunoflourescence Kit 50 Analyses 7182
topogen TG1035 Topoisomerase II Immunoprecipitation Kit 50 Analyses 7182
topogen TG2000G-1KIT Gyrase/Relaxed DNA Assay Kit 100 Assays 8442
topogen TG2000G-3KIT Gyrase/Relaxed DNA Assay Kit 500 Assays 16182
topogen TG2000G-5KIT Gyrase/Relaxed DNA Assay Kit 1000 Assays 25182
topogen TG2000G-7KIT Gyrase/Relaxed DNA Assay Kit 2000 Assays 35982
topogen TG2000H-1 Human Topoisomerase II 250 Units 4482
topogen TG2000H-2 Human Topoisomerase II 500 Units 6282
topogen TG2005H-Y723F Mutant Human Topo I 10 ug 5382
topogen TG2005H-RC1 Human Topoisomerase I 500 Units 4482
topogen TG2000G-1 E. Coli DNA Gyrase （Type II Topoisomerase） 100 Units 3582
topogen TG2000G-3 E. Coli DNA Gyrase （Type II Topoisomerase） 500 Units 8982
topogen TG2000G-5 E. Coli DNA Gyrase （Type II Topoisomerase） 1000 Units 15642
topogen TG2000G-7 E. Coli DNA Gyrase （Type II Topoisomerase） 2000 Units 24282
topogen TG2000EC-1 Topoisomerase IV 250 Units 5382
topogen TG2000EC-3 Topoisomerase IV 500 Units 8982
topogen TG2000EC-5 Topoisomerase IV 1000 Units 16182
topogen TG2004H-1 Human Tdp1 250 Units 5382
topogen TG2004H-2 Human Tdp1 500 Units 7182
topogen TG2000GSA-1 S. Aureus DNA Gyrase 100 Units 2502
topogen TG2000GSA-3 S. Aureus DNA Gyrase 500 Units 8982
topogen TG2000GSA-5 S. Aureus DNA Gyrase 1000 Units 14382
topogen TG2013-1 Kinetoplast DNA （kDNA） 25ug 3582
topogen TG2013-2 Kinetoplast DNA （kDNA） 50ug 5382
topogen TG2013-3 Kinetoplast DNA （kDNA） 250ug 12582
topogen TG2023-2 pRYG DNA 20 ug 3582
topogen TG2030-2 pHOT-1 DNA 20 ug 3582
topogen TG2036-1 Supercoiled pBR322 DNA （modified） 50 ug 4122
topogen TG2036-2 Supercoiled pBR322 DNA （modified） 250 ug 10062
topogen TG2037-1 Relaxed pBR322 DNA 50 ug 4482
topogen TG2037-2 Relaxed pBR322 DNA 250 ug 10782
topogen TG2010-1 Monoclonal Antibody to Human Topoisomerase II Alpha 50 Units 6642
topogen TG2010-3 Polyclonal Antibody to Human Topoiosomerase II Beta 250 Units 5922
topogen TG2010-4 Polyclonal Antibody to Human Topoisomerase III Beta 250 Units 5922
topogen TG2011-1 Polyclonal Antibody to Human Topoisomerase II Alpha 250 Units 5922
topogen TG2012 Polyclonal Antibody to Human Topoiosomerase I （Scleroderma Patient Serum） 500 Units 5382
topogen TG2012-2 Polyclonal to Human Topoisomerase I （serum） 250 ul 4122
topogen TG2012-4 Monoclonal to Human Topoisomerase I 100 ug 5382
topogen TG2014 Polyclonal to Yeast Topoisomerase II （Serum） 250 ul 5382
topogen TG2015 Monoclonal to Human Topoisomerase III Alpha 50 ul 5382
topogen TG2016 Polyclonal to Human Tdp1 250 Units 5922
topogen TG2011-2 Topoisomerase II Alpha C-Terminal Peptide 50 ug 3222
topogen TG4100 Topotecan Hydrochloride （Hycamtin） 0.25 ml 3582
topogen TG4101 Irinotecan （CPT-11） 0.25 ml 1962
topogen TG4102 9-Nitro-20 （S） Camptothecin （Rubitecan） 0.25ml 1962
topogen TG4103 10-Hydroxy Camptothecin 0.25 ml 1962
topogen TG4104 9-Amino Camptothecin 0.25 ml 1962
topogen TG4110 Camptothecin Lyophilized 1782
topogen TG4120 Doxorubicin （Adriacin） 0.25 ml 1962
topogen TG4130 Daunomycin 0.25 ml 1782
topogen TG4140 Etoposide （VP16） Lyophilized 1782
topogen TG4170 Ellipticine 0.25 ml 1782
topogen TG4180 Ciproflaxin 50 ul 1242
topogen TG4150 Amsacrine （m-AMSA） 0.25 ml 1782
topogen TG4160 Genistein 0.25 ml 1782
topogen TG2011-3 Human Topoisomerase II Alpha 20 Units 3582
topogen TG2011-4 Topoisomerase I Western Blot 20 Units 3042
topogen TGVI DNA Topoisomerase Protocols Volume I N/A 2322
topogen TGVII DNA Topoisomerase Protocols Volume II N/A 2322
topogen TG4010 10X Topoisomerase I Assay Buffer 1 ml 882
topogen TG4030 5X Gyrase Assay Buffer 1 ml 882
topogen TG4032 10X Topoisomerase IV Assay Buffer 1 ml （A）, 100 ul （B） 882
topogen TG4037 5X Universal Stop Buffer 1 ml 882
topogen TG4038 10X Gel Loading Buffer 1 ml 882
topogen TG4039 10X Proteinase K 0.5ml 882
topogen TG4040 Incomplete Topoisomerase II Assay Buffer 1 ml （A）, 1 ml （B） 882
topogen TG4060 10% Sodium Dodecyl Sulfate 1 ml 882
topogen TG4065 Sarkosyl （20%） 5 ml 1782
topogen TG4090 Topoisomerase II Dilution Buffer 1 ml 882
topogen TG4095 Topoisomerase I Dilution Buffer 1 ml 882
topogen TG2017-1 Linear kDNA 10 ug 3042
topogen TG2020-1 Decatenated kDNA 10 ug 3402
topogen TG2028-1 Linear pRYG DNA 10 ug 1962
topogen TG2025-1 Relaxed pRYG DNA 10 ug 2682
topogen TG2035-1 Relaxed pHOT-1 DNA 10 ug 2322

                                                   

 
我们公司zui大优势是强大的采购，
1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，
2： 货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，
3：提供加急服务，一般1-2周到货，超过时限加急费全免
4：价格公道，绝大部分价格有优势，当然不能保证100%产品都有价格优势，
5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司
6：我们还是santa,Advanced Biotechnologies Inc;Athens Research & Technology, bangs, BBInternational, crystalchem, dianova, FD Neurotechnologies, Inc. FormuMax Scientific, Inc; Genebridege; Glycotope Biotechnology GmbH; iduron; Innovative Research of America； Ludger ； neuroprobe; omicronbio; Polysciences; prospecbi; QA-BIO；quickzyme；RESEARCH DIETS, INC；sterlitech；sysy；TriLink BioTechnologies, Inc；TOPOGEN -biochem;zyagen;……几十家国外公司代理
7：我们还从事invitrogen，qiagen; abcam ;sigma;neb; roche;merck; rnd; BD; GE; pierce; BioLegend….等*批发                                         

  TOPOGEN 代理， ，  代理， 代理中国代理， 上海代理， 北京代理， 国内代理， 大陆代理， 代理

 

上海金畔生物科技有限公司
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地址： 

喜获Inspitalis中国地区代理！

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上海金畔喜获Inspitalis中国地区代理！

上海金畔与Inspitalis签订协议，正式成为Inspitalis在中国地区的代理。

 

 

自1995年以来，我们一直向制药行业和学术界提供拓扑异构酶产品和服务，以帮助抗感染和抗癌市场的研究。Inspiralis的前身是John Innes Enterprises，它是根据拓扑异构酶研究领域的托尼·麦克斯韦教授的研究而建立的。
我们位于约翰尼恩斯中心和诺维奇研究园东安格利亚大学的学术研究中心附近的实验室。我们与Maxwell教授的学术研究小组保持密切联系，帮助我们保持在拓扑异构酶领域的前沿。我们员工的经验以及与学术团体的联系使我们能够提供高水平的支持，以确保研究人员能够使用我们的产品获得最佳结果。生产酶和底物的科学家也提供了技术支持。

 

关于“正牌代理商”栏目的声明        

“正牌代理商”栏目设立的初衷是为了让读者在购买产品时能更快找到厂家的代理商，不用一张一张地翻名片，也不用担心买到水货和假货。基于这个初衷，我们收录了多个品牌的代理商，并在签订合同时进行了严格的审核。各厂家或代理商以合同的形式对其代理信息的真实性和准确性进行了保证。       然而，由于生物产品市场的复杂性，尽管我们进行了严格的审核，也不能保证信息的*准确和实时更新。基于此，今后我们会尽我们最大的努力去进行更严格的审核，也希望各厂家和代理商配合和谅解。

 

topogen TG2000H-2现货说明书

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topogen TG2000H-2现货说明书

人拓扑异构酶IIα酶–500单位

Human Topoisomerase IIα Enzyme – 500 Units

Human Topoisomerase IIα Enzyme

纯化的人拓扑异构酶IIα（Top2a）过表达，并在SDS-PAGE上纯化至单一条带的均一性。拓扑异构酶II高度纯化，无核酸酶污染。

人拓扑异构酶IIα质量控制试验

核酸污染：通过检测线性kDNA和线性质粒DNA的形成来检测。在37°C（在top2a测定条件下，含或不含ATP）下，将1µg连环KDNA或超螺旋pUC19 DNA孵育4小时。

通过在抑制人类topo IIa活性的条件下测定超螺旋DNA的松弛来评估与topo I的交叉污染。将过量纯化的酶与pRYG DNA在37°C条件下，在不存在ATP（含或不含Mg++）的情况下孵育2小时。在这些条件下，不得检测到pRYG超螺旋DNA的松弛。

当SDS-PAGE凝胶过载纯蛋白时，可以看到170kDa的主带。最终馏分中没有180kDa拓朴II形式。（不同批次的蛋白质浓度不同，但通常在5-50μg/ml的范围内）人类拓扑异构酶IIα的供应量为2-10单位/ul（37°C时，1个单位将在30分钟内降解0.2μg KDNA）。注意，单位定义基于优选的DNA底物kDNA（动粒DNA）。我们建议将kDNA底物与这种酶一起使用，因为质粒DNA弛豫测定几乎不那么敏感。活性基于kDNA脱链（而非质粒DNA松弛）进行验证。

top2酶的最终部分来自FPLC柱，在以下缓冲液中：10%甘油、50mM Tris（pH 7.7）、1mM EDTA和EGTA、650mM NaCl。

人拓扑异构酶IIα测定条件和性能质控

使用在拓扑II反应缓冲液（50mM Tris-Cl，pH 8.0，120mM KCl，10mM MgCl2，0.5mM ATP，0.5mM二硫苏糖醇）中最终体积为20-30µl的动载体DNA（KDNA）底物和0.2µg KDNA进行链连接测定。用5µl（每20µl反应体积）的终止缓冲液（5%沙科西、0.0025%溴酚蓝、25%甘油）终止反应。反应产物在含有0.5ug/ml溴化乙锭的1%琼脂糖凝胶上分析。当凝胶运行时，用手持紫外光源可以容易地监测2.5kb DNA微环脱链产物的分辨率。

酶在干冰上运输，应在-70°C下储存。我们还建议在第一次解冻后等分酶（反复冷冻/解冻会导致活性丧失）；酶活性稳定1-2天（不更长）。

inspiralis TRT101现货说明书

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上海金畔inspiralis TRT101现货说明书

人类拓扑异构酶 I

人类拓扑异构酶 I

人类拓扑异构酶 I 是通过在杆状病毒感染的昆虫细胞 ( Spodoptera frugiperda) 中过表达该酶并通过改编自 Stewart等人，1996 的方法对其进行纯化来制备 的。该酶在稀释缓冲液中提供 μl。

储存于-80°C。建议将酶分装以避免重复冻融循环。

货号

 产品 价格 数量
HT101 人体拓扑 I
100 U		 85 英镑
HT105 人体拓扑 I
500 U		 250 英镑
HT110 人体拓扑 I
1000 U		 416 英镑
HT120 人体拓扑 I
2000 U		 690 英镑

人拓扑异构酶 I 松弛检测试剂盒

除了用于松弛反应的测定和稀释缓冲液外，它们还包含酶和超螺旋 DNA 底物。1 U 的人类 topo I 将在 37°C 下 30 分钟内松弛 0.5 µg 超螺旋 pBR322 DNA。 

货号

 产品 价格 数量
HTR101 人 Topo I 松弛检测试剂盒 1
100 U 人 topo I 和 50 µg 超螺旋 DNA		 210 英镑
HTR102 人 Topo I 松弛检测试剂盒 2
500 U 人 topo I 和 250 µg 超螺旋 DNA		 665 英镑
HTR103 人 Topo I 松弛检测试剂盒 3
1000 U 人 topo I 和 500 µg 超螺旋 DNA		 1,242 英镑
HTR104 人 Topo I 松弛检测试剂盒 4
2000 U 人 topo I 和 1 mg 超螺旋 DNA		 1,944 英镑

用于细胞提取物的人类拓扑异构酶 I 检测试剂盒

这些试剂盒设计用于检测含有人类拓扑 I 的细胞提取物和部分纯化的部分。它们包含超螺旋 DNA 底物、检测缓冲液、稀释缓冲液和松弛 DNA 标记。

试剂盒成分基于标准人体拓扑 I 测定，每次测定包含 500 ng 底物 DNA。

货号

 产品 价格 数量
TRAK1 人 Topo I 细胞提取物检测试剂盒 1
包含足够的检测缓冲液、超螺旋和松弛标记 DNA，可用于 100 次检测		 175 英镑
TRAK2 Human Topo I 细胞提取物检测试剂盒 2
包含足够的检测缓冲液、超螺旋和松弛标记 DNA，可进行 500 次检测		 442 英镑

该试剂盒提供了足够的人类拓扑 I 酶、质粒 DNA 底物、缓冲液和其他检测成分*，可进行 100 次检测。该酶在稀释缓冲液中以 10 U/μl 的浓度提供。该试剂盒还提供了足够用于一块 96 孔板的洗涤缓冲液。这些缓冲液以 20X 浓缩液的形式提供，使用前必须用超纯水稀释。

有关此检测的更多信息，请参见“高/中通量检测”下的“服务”页面。

套件附带有限许可，仅供个人使用。 

货号

 产品 价格 数量
TRT101 HTS 检测试剂盒 – Human Topo I 1-5 试剂盒
，每板 100 次检测		 465 英镑
TRT102 HTS 检测试剂盒 – Human Topo I 6+ 试剂盒
，每板可进行 100 次检测		 375 英镑

inspiralis DNA Unwinding Assay Kit

发表于由

inspiralis自1995以来，一直为制药行业和学术界提供抗感染、**研究产品和服务。inspiralis专门供应（包括**拓扑异构酶旋转酶，拓扑异构酶IV，人类拓扑异构酶I和II和基板超螺旋和轻松的pBR322和kDNA）和提供拓扑异构酶抑制剂筛选试验（低和高通量），inspiralis的目标是提升研究抗感染、**市场研究效率，以便更好的为客户谋得福利。

inspiralis DNA Unwinding Assay Kit

DNA Unwinding Assay Kit

产品描述（产品编号：Duksr001、Dukr002、Duks003）在稀释缓冲液中以2 U/L的低浓度供应酶。储存于-80C（稳定3个月，未稀释）。建议对酶进行校准，以避免重复的冻融循环。对于体外实验室搜索仅使用。

展开分析典型反应包括15μL（2X）分析缓冲液、0.5μL PBR322（1μg/μL）加上小麦胚芽拓扑I（2.5 U）和化合物（视情况而定），总体积为30μL。反应在37℃下培养30分钟。加入20μl水和50μl丁醇，反应短暂旋转，离心分离。萃取后，将水层（下层）与等体积的STEB混合，用氯方/异戊醇萃取，并对琼脂糖凝胶进行分析，凝胶可在氯喹（CQ）存在或不存在下运行。超冷法测定BR322。TOPO I单独使质粒松弛（通道3），但在TOPO I和ETBR的存在下，质粒运行接近过筛DNA（通道4）。

 

质量控制
核酸内切酶分析：用1μg的小麦胚芽拓扑异构酶Ⅰ在6℃下孵育0.5 g，在琼脂糖凝胶电泳中检测到松弛DNA为开放的圆形或线性形式。

 

 

 

TopoGEN2022价格表，欢迎选购！

发表于由

美国TopoGEN公司成立于1991年，是一家诊断公司，由一批在拓扑异构酶领域工作和在基础、临床、应用及相关方面研究经验丰富的研究者创建，为以药物开发为基础的拓扑异构酶和机制研发人员提供创新产品和服务。

药物筛选试剂盒可以分析拓扑异构酶活性药物，用于癌症化疗和抗菌方面。

TopoGEN公司还能提供原核和真核化验拓扑异构酶 (I型，II，IV和旋转酶)检测试剂盒，体外体内诊断均可应用。

TopoGEN公司还提供DNA载体、DNA裂解目标、拓扑异构酶抑制剂、拓扑异构酶抗体和相关试剂盒等完整产品线。


上海金畔TopoGEN2022价格表，欢迎选购！

Catalog No. Description Size 价格 品牌 Dry ice
TG2000H-1 Human Topoisomerase IIa 250 units 5015 TopoGEN Dry ice
TG2000H-2 Human Topoisomerase IIa 500 units 7395 TopoGEN Dry ice
TG2000H-3 Human Topoisomerase IIa 1000 units 10965 TopoGEN Dry ice
TG2000H-4 Human Topoisomerase IIa 2000 units 14875 TopoGEN Dry ice
TG2005H-RC1 Human Topoisomerase I  250 units 4760 TopoGEN Dry ice
TG2005H-RC2 Human Topoisomerase I 500 units 6630 TopoGEN Dry ice
TG2005H-RC3 Human Topoisomerase I  3000 units 18530 TopoGEN Dry ice
TG2005H-Y723F Mutant Human Topoisomerase I (Y723F) 10ug 7395 TopoGEN Dry ice
TG2000G-1 E. coli DNA Gyrase 100 units 4930 TopoGEN Dry ice
TG2000G-3 E. coli DNA Gyrase 500 units 11135 TopoGEN Dry ice
TG2000G-5 E. coli DNA Gyrase 1000 units 18530 TopoGEN Dry ice
TG2000G-7 E. coli DNA Gyrase 2000 units 29835 TopoGEN Dry ice
TG2000GSA-1 S. aureus DNA Gyrase 100 units 5865 TopoGEN Dry ice
TG2000GSA-3 S. aureus DNA Gyrase 500 units 11135 TopoGEN Dry ice
TG2000GSA-5 S. aureus DNA Gyrase 1000 units 16745 TopoGEN Dry ice
TG2000EC-1 E. coli Topoisomerase IV 250 units 6630 TopoGEN Dry ice
TG2000EC-3 E. coli Topoisomerase IV 500 units 10540 TopoGEN Dry ice
TG2000EC-5 E. coli Topoisomerase IV 1000 units 16745 TopoGEN Dry ice
TG1050-1 Topoisomerase Type IIa Nuclear Extraction/Assay Kit 100 assays 6970 TopoGEN
TG1001-1 Topoisomerase II Assay Kit (20ug kDNA) 100 assays 4845 TopoGEN
TG1001-2 Topoisomerase II Assay Kit (50ug kDNA) 250 assays 6545 TopoGEN
TG1001-1A Topoisomerase II Assay Kit (20ug kDNA + 100u Human Topo Iia) 100 assays 7395 TopoGEN Dry ice
TG1001-2A Topoisomerase II Assay Kit (50ug kDNA + 250u  Human Topo Iia) 250 assays 10795 TopoGEN Dry ice
TG1001-3A Topoisomerase II Assay Kit (100ug kDNA + 500u Human Topo Iia) 500 assays 12665 TopoGEN Dry ice
TG1009-1 Topoisomerase II Drug Screening Kit (25ug SC DNA) 100 assays 5525 TopoGEN
TG1009-2 Topoisomerase II Drug Screening Kit (62.5ug SC DNA) 250 assays 7395 TopoGEN
TG1009-1A Topoisomerase II Drug Screening Kit (25ug SC DNA + 100u Topo Iia) 100 assays 6715 TopoGEN Dry ice
TG1009-2A Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 250u Topo Iia) 250 assays 10795 TopoGEN Dry ice
TG1009-3A Topoisomerase II Drug Screening Kit (62.5ug SC DNA + 500u Topo Iia) 250 assays 12665 TopoGEN Dry ice
TG1019-1 Topoisomerase II Drug Screening Kit (20ug kDNA) 100 assays 5525 TopoGEN
TG1019-2 Topoisomerase II Drug Screening Kit (50ug kDNA) 250 assays 7395 TopoGEN
TG1019-1A Topoisomerase II Drug Screening Kit (20ug kDNA + 100u Human Topo Iia) 100 assays 7395 TopoGEN Dry ice
TG1019-2A Topoisomerase II Drug Screening Kit (50ug kDNA + 250u  Human Topo Iia) 250 assays 11135 TopoGEN Dry ice
TG1019-3A Topoisomerase II Drug Screening Kit (50ug kDNA + 500u  Human Topo Iia) 250 assays 12665 TopoGEN Dry ice
TG1022 Topo II In Vivo Link Kit 200 assays 8075 TopoGEN Dry ice
TG1007-1 E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA) 100 assays 5525 TopoGEN
TG1007-2 E. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA) 250 assays 7395 TopoGEN
TG1007-1A E. coli Topoisomerase IV Drug Screening Kit (25ug SC DNA + 100u EC Topo IV) 100 assays 6630 TopoGEN Dry ice
TG1007-2A E. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA + 250u EC Topo IV) 250 assays 11135 TopoGEN Dry ice
TG1007-3A E. coli Topoisomerase IV Drug Screening Kit  (62.5ug SC DNA + 500u EC Topo IV) 250 assays 14875 TopoGEN Dry ice
TG1008-1 E. coli Topo IV Drug Screening Kit (20ug kDNA) 100 assays 5525 TopoGEN
TG1008-2 E. coli Topo IV Drug Screening Kit (50ug kDNA) 250 assays 7395 TopoGEN
TG1008-1A E. coli Topo IV Drug Screening Kit (20ug kDNA + 100u E. coli Topo IV) 100 assays 6630 TopoGEN Dry ice
TG1008-2A E. coli Topo IV Drug Screening Kit (50ug kDNA + 250u E. coli Topo IV) 250 assays 12240 TopoGEN Dry ice
TG1008-3A E. coli Topo IV Drug Screening Kit (50ug kDNA + 500u E. coli Topo IV) 250 assays 14875 TopoGEN Dry ice
TG1040-1 Topoisomerase Type I Nuclear Extraction/Assay Kit 100 assays 6885 TopoGEN
TG1015-1 Topoisomerase I Assay Kit (25ug SC DNA) 100 assays 4760 TopoGEN
TG1015-2 Topoisomerase I Assay Kit (62.5ug SC DNA) 250 assays 6460 TopoGEN
TG1015-1A Topoisomerase I Assay Kit (25ug SC DNA +100u Human Topo I) 100 assays 6630 TopoGEN Dry ice
TG1015-2A Topoisomerase I Assay Kit (62.5ug SC DNA + 250u Human Topo I) 250 assays 10115 TopoGEN Dry ice
TG1015-3A Topoisomerase I Assay Kit (125ug SC DNA + 500u Human Topo I) 500 assays 12070 TopoGEN Dry ice
TG1018-1 Topoisomerase I Drug Screening Kit (25ug SC DNA) 100 assays 5355 TopoGEN
TG1018-2 Topoisomerase I Drug Screening Kit (62.5ug SC DNA) 250 assays 6630 TopoGEN
TG1018-1A Topoisomerase I Drug Screening Kit (25ug SC DNA + 100u Human Topo I) 100 assays 6885 TopoGEN Dry ice
TG1018-2A Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 250u Human Topo I) 250 assays 10540 TopoGEN Dry ice
TG1018-3A Topoisomerase I Drug Screening Kit (62.5ug SC DNA + 500u Human Topo I) 250 assays 12240 TopoGEN Dry ice
TG1021 Topoisomerase I In Vivo Link Kit 200 assays 8330 TopoGEN Dry ice
TG1017-1 Human Topoisomerase I High Load Kit 500 units 8330 TopoGEN Dry ice
TG1017-2 Human Topoisomerase I High Load Kit 1000 units 10115 TopoGEN Dry ice
TG1010-1 In Vitro SDS-K+ Precipitation Kit 100 assays 6885 TopoGEN
TG1010-2 In Vitro SDS-K+ Precipitation Kit 250 assays 8755 TopoGEN
TG1025 DNA Intercalator/Unwinding Kit 50 experiments 7905 TopoGEN Dry ice
TG1011-1 In Vivo SDS-K+ Precipitation Kit  100 assays 4165 TopoGEN Dry ice
TG1011-2 In Vivo SDS-K+ Precipitation Kit  250 assays 6035 TopoGEN Dry ice
TG1020-0 Human Topoisomerase I ICE Assay Kit (No Antibody) 24 Tests 6290 TopoGEN
TG1020-1 Human Topoisomerase I ICE Assay Kit 24 Tests 9775 TopoGEN
TG1020-2a Human Topoisomerase IIa ICE Assay Kit 24 Tests 9265 TopoGEN
TG1020-2b Human Topoisomerase IIb ICE Assay Kit 24 Tests 9265 TopoGEN
TG1020-3a Human Topoisomerase IIIa ICE Assay Kit 24 Tests 9265 TopoGEN
TG1020-3b Human Topoisomerase IIIb ICE Assay Kit 24 Tests 9265 TopoGEN
TG1030-1 Human Topoisomerase I EMSA Kit 25 assays 5525 TopoGEN
TG1030-2 Human Topoisomerase II EMSA Kit 25 assays 7395 TopoGEN
TG1003 DNA Gyrase Assay Kit 100 assays 5015 TopoGEN
TG2000G-1KIT Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase) 100 assays 6035 TopoGEN Dry ice
TG2000G-3KIT Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase) 500 assays 15640 TopoGEN Dry ice
TG2000G-5KIT Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase) 1000 assays 26010 TopoGEN Dry ice
TG2000G-7KIT Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase) 2000 assays 46665 TopoGEN Dry ice
TG2001G-1KIT Purified E. coli DNA Gyrase Drug Screening Kit (50ug Relaxed DNA + 100u Gyrase) 100 assays 6375 TopoGEN Dry ice
TG2001G-3KIT Purified E. coli DNA Gyrase Drug Screening Kit (250ug Relaxed DNA + 500u Gyrase) 500 assays 16745 TopoGEN Dry ice
TG2001G-5KIT Purified E. coli DNA Gyrase Drug Screening Kit (500ug Relaxed DNA + 1000u Gyrase) 1000 assays 29835 TopoGEN Dry ice
TG2001G-7KIT Purified E. coli DNA Gyrase Drug Screening Kit  (1mg Relaxed DNA + 2000u Gyrase) 2000 assays 48535 TopoGEN Dry ice
TG2011-5 Mouse Monoclonal Antibody to Human Topo IIB 100 units 5525 TopoGEN Dry ice
TG1024-A Topoisomerase IIa Band Shift Kit 25 assays 8075 TopoGEN Dry ice
TG1024-B Topoisomerase IIb Band Shift Kit 25 assays 8075 TopoGEN Dry ice
TG2010-1 Mouse Monoclonal Antibody to Human Topoisomerase IIα  50 units 5355 TopoGEN Dry ice
TG2011-1 Rabbit Polyclonal Antibody to Human Topoisomerase IIα 250 units 6035 TopoGEN
TG2010-3 Rabbit Polyclonal Antibody to Human Topoisomerase IIb 250 units 6035 TopoGEN
TG2012 Human Antibody to Human Topoisomerase I (Scleroderma Serum) 500 units 6035 TopoGEN Dry ice
TG2012-2 Rabbit Polyclonal Antibody to Human Topoisomerase I (Rabbit Serum) 250 ul 5525 TopoGEN Dry ice
TG2012-4 Mouse Monoclonal Antibody to Human Topoisomerase I 100 ug 5525 TopoGEN Dry ice
TG2014 Rabbit Polyclonal Antibody to Yeast Topo II (Serum) 250 ul 5525 TopoGEN Dry ice
TG2010-4 Rabbit Polyclonal Antibody to Human Topoisomerase IIIb 250 units 5525 TopoGEN Dry ice
TG2015 Mouse Monoclonal Antibody to Human Topoisomerase IIIα 50 ul 6375 TopoGEN Dry ice
TG2011-2 Topoisomerase IIα C-terminal Peptide 50 ug 6375 TopoGEN
TG2011-3 Topoisomerase IIα MW Marker 20 units 6375 TopoGEN Dry ice
TG2011-4 Topoisomerase I WB Marker  20 units 6375 TopoGEN Dry ice
TG1030 Topoisomerse II Immunofluorescence Kit >50 analyses 8245 TopoGEN
TG1035 Topoisomerase II Immunoprecipitation Kit >50 analyses 8245 TopoGEN
TG1004-1 Human TDP1 Assay Kit  (Oligo) 100 assays 6970 TopoGEN
TG1004-1A Human TDP1 Assay Kit  (DNA/TopComplex) 100 assays 8415 TopoGEN
TG1004-2 Human TDP1 Assay Kit (Oligo) 250 assays 8415 TopoGEN
TG1004-2A Human TDP1 Assay Kit (DNA/Top Complex) 250 assays 10115 TopoGEN
TG2004H-1 Human TDP1 Protein 250 units 6035 TopoGEN Dry ice
TG2004H-2 Human TDP1 Protein 500 units 8075 TopoGEN Dry ice
TG2016 Rabbit Polyclonal Antibody to Human TDP1 250 units 6035 TopoGEN
TG2038-1 Human TDP2 DNA Substrate 10 ug 6035 TopoGEN
TG2038-3 Human TDP2 DNA Substrate 50 ug 8075 TopoGEN
TG1005-1 Human TDP2 Assay Kit 100 assays 8075 TopoGEN
TG1005-2 Human TDP2 Assay Kit 250 assays 10115 TopoGEN
TG2003H-1 Human TDP2 Protein 25 ug 6715 TopoGEN Dry ice
TG2003H-2 Human TDP2 Protein 50 ug 8670 TopoGEN Dry ice
TG2013-1 Kinetoplast DNA (catenated) 25 ug 3655 TopoGEN
TG2013-2 Kinetoplast DNA (catenated) 50 ug 5525 TopoGEN
TG2013-3 Kinetoplast DNA (catenated) 250 ug 16660 TopoGEN Dry ice
TG2018-1 Linear Kinetoplast DNA Marker 10 ug 2295 TopoGEN
TG2020-1 Decatenated Kinetoplast DNA Marker 10 ug 2295 TopoGEN
TG2023-2 pRYG DNA (topo II target site) 20 ug 5525 TopoGEN
TG2025-1 Relaxed pRYG DNA Marker 10 ug 4165 TopoGEN
TG2028-1 Linear pRYG DNA Marker 10 ug 3655 TopoGEN
TG2030-1 Supercoiled pHOT-1 DNA 25 ug 3655 TopoGEN
TG2030-2 Supercoiled pHOT-1 DNA 50 ug 4845 TopoGEN
TG2030-3 Supercoiled pHOT-1 DNA 250 ug 11135 TopoGEN
TG2030-4 Supercoiled pHOT-1 DNA 500 ug 16745 TopoGEN
TG2035-1 Relaxed pHOT-1 DNA 25 ug 3995 TopoGEN
TG2035-2 Relaxed pHOT-1 DNA 50 ug 5525 TopoGEN
TG2035-3 Relaxed pHOT-1 DNA 250 ug 13005 TopoGEN
TG2035-4 Relaxed pHOT-1 DNA 500 ug 18530 TopoGEN
DR3000 HRHeLa-Sce HR based Kit in HeLa Requiring I-Sce1 Transfection (50ug DNA) 77180 TopoGEN
DR3000 HRHeLa-Sce2 HR based Kit in HeLa Requiring I-Sce1 Transfection (100ug DNA) 82110 TopoGEN
DR3000 HRHeLa-Sce3 HR based Kit in HeLa Requiring I-Sce1 Transfection (250ug DNA) 91885 TopoGEN
DR3000 HRHeLa-CPR HR based Kit in HeLa Requiring CRISPR Transfection (50ug DNA) 81515 TopoGEN
DR3000 HRHeLa-CPR2 HR based Kit in HeLa Requiring CRISPR Transfection (100ug DNA) 86445 TopoGEN
DR3000 HRHeLa-CPR3 HR based Kit in HeLa Requiring CRISPR Transfection (250ug DNA) 96135 TopoGEN
DR3000 HRCN1.4-Sce HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (50ug DNA) 92905 TopoGEN
DR3000 HRCN1.4-Sce2 HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (100ug DNA) 97750 TopoGEN
DR3000 HRCN1.4-Sce3 HR based Kit in Mouse Neuronal Cells Requiring I-Sce1 Transfection (250ug DNA) 107525 TopoGEN
DR3000 HRSH-Sce HR in human Neuroblastoma Cells req. I-Sce1 Transfection (50ug DNA) 92905 TopoGEN
DR3000 HRSH-Sce2 HR in human Neuroblastoma Cells req. I-Sce1 Transfection (100ug DNA) 97750 TopoGEN
DR3000 HRSH-Sce3 HR in human Neuroblastoma Cells req. I-Sce1 Transfection (250ug DNA) 107525 TopoGEN
DR3000 HR3T3-Sce HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (50ug DNA) 77180 TopoGEN
DR3000 HR3T3-Sce2 HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (100ug DNA) 82110 TopoGEN
DR3000 HR3T3-Sce3 HR based Kit in MOUSE 3T3 Cells  Requiring I-Sce1 Transfection (250ug DNA) 91885 TopoGEN
DR3000 Custom-Sce Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (50ug DNA) 96815 TopoGEN
DR3000 Custom-Sce2 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (100ug DNA) 106505 TopoGEN
DR3000 Custom-Sce3 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.)  Includes HRHeLa cell line control (250ug DNA) 116280 TopoGEN
DR3000A Custom-Sce Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (50ug DNA) 81515 TopoGEN
DR3000A Custom-Sce2 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (100ug DNA) 86445 TopoGEN
DR3000A Custom-Sce3 Customer makes own cell line hosting HR Reporter (I-Sce Transfectn req.) No HRHeLa cell line control (250ug DNA) 96135 TopoGEN
DR5001 HNHela-Sce NHEJ Based HeLa Requires I-Sce1 Transfection (50ug DNA) 77180 TopoGEN
DR5001 HNHela-Sce2 NHEJ Based HeLa Requires I-Sce1 Transfection (100ug DNA) 82110 TopoGEN
DR5001 HNHela-Sce3 NHEJ Based HeLa Requires I-Sce1 Transfection (250ug DNA) 91885 TopoGEN
DR5000 HNSH-Sce NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (50ug DNA) 92820 TopoGEN
DR5000 HNSH-Sce2 NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (100ug DNA) 97750 TopoGEN
DR5000 HNSH-Sce3 NHEJ Based in Human Neuroblastoma Line requires I-Sce1 transfection (250ug DNA) 107525 TopoGEN
DR5000 HNCN1.4-Sce NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (50ug DNA) 92820 TopoGEN
DR5000 HNCN1.4-Sce2 NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (100ug DNA) 97750 TopoGEN
DR5000 HNCN1.4-Sce3 NHEJ Based in Mouse Neuronal CN1.4 cells requires I-Sce1 transfection (250ug DNA) 107525 TopoGEN
DR5000 HN3T3-Sce NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (50ug DNA) 77180 TopoGEN
DR5000 HN3T3-Sce2 NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (100ug DNA) 82110 TopoGEN
DR5000 HN3T3-Sce3 NHEJ Based Mouse 3T3 cells Requires I-Sce1 Transfection (250ug DNA) 91885 TopoGEN
DR5000 Custom-Sce Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (50ug DNA) 111435 TopoGEN
DR5000 Custom-Sce2 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (100ug DNA) 116280 TopoGEN
DR5000 Custom-Sce3 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  Includes iHNHeLa cell line control (250ug DNA) 126055 TopoGEN
DR5000A Custom-Sce Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (50ug DNA) 81515 TopoGEN
DR5000A Custom-Sce2 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (100ug DNA) 86445 TopoGEN
DR5000A Custom-Sce3 Customer creates cell line hosting NHEJ Reporter (I-Sce Transfectn req.)  NO iHNHeLa cell line control (250ug DNA) 96135 TopoGEN
DR3050 HR Pooled Cell analysis:  50ug DR-HRGFP, 50ug pSce1, 25ug e-GFP, Puromycin 68425 TopoGEN
DR3250 HeLa Cell RepHR Pooled Cell analysis:  250ug DR-HRGFP, 250ug pSce1, 25ug e-GFP, Puromycinorters to detect DNA Hypomethylating Effectors 136765 TopoGEN
DR3500 HR Pooled Cell analysis:  500ug DR-HRGFP, 500ug pSce1, 25ug e-GFP, Puromycin 156315 TopoGEN
DR31000 HR Pooled Cell analysis:  1000ug DR-HRGFP, 10000ug pSce1, 25ug e-GFP, Puromycin 195415 TopoGEN
DRT5050 Detailed protocols with 50ug of cNHEJ plasmid, 50ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 68425 TopoGEN
DRT5250 Detailed protocols with 250ug of cNHEJ plasmid, 250ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 136765 TopoGEN
DRT5500 Detailed protocols with 500ug of cNHEJ plasmid, 500ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 156315 TopoGEN
DRT51000 Detailed protocols with 1000ug of cNHEJ plasmid, 1000ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 195415 TopoGEN
DRA5050 Detailed protocols with 50ug of Alt-NHEJ plasmid, 50ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 76245 TopoGEN
DRA5250 Detailed protocols with 250ug of Alt-NHEJ plasmid, 250ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 136765 TopoGEN
DRA5500 Detailed protocols with 500ug of Alt-NHEJ plasmid, 500ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 156315 TopoGEN
DR51000 Detailed protocols with 1000ug of Alt-NHEJ plasmid, 1000ug I-Sce1 plasmid, 50ug eGFP transfection control plasmid 195415 TopoGEN
TG4110 Camptothecin 10mM 0.25ml 1615 TopoGEN Dry ice
TG4120 Doxorubicin 10mM 0.25ml 1615 TopoGEN Dry ice
TG4130 Daunomycin 10mM 0.25ml 1615 TopoGEN Dry ice
TG4140 Etoposide 10mM 0.25ml 1615 TopoGEN
TG4150 m-AMSA 10mM 0.25ml 1615 TopoGEN Dry ice
TG4160 Genistein 10mM 0.25ml 1615 TopoGEN Dry ice
TG4170 Ellipticine 10mM 0.25ml 1615 TopoGEN Dry ice
TG4180 Ciproflaxin 50 ul 1615 TopoGEN Dry ice
TG4100 Topotecan 10mM 0.25ml 1615 TopoGEN Dry ice
TG4101 Irinotecan 10mM 0.25ml 1615 TopoGEN Dry ice
TG4102 9-Nitro-20 (S) Camptothecin 10mM 0.25ml 1615 TopoGEN Dry ice
TG4103 10-Hydroxy Camptothecin 10 mM 0.25ml 1615 TopoGEN Dry ice
TG4104 9-amino-CPT 10mM (0.25ml) 0.25ml 1615 TopoGEN Dry ice
TG4010 Topo I Assay Buffer 10x 1ml 1615 TopoGEN
TG4030 Gyrase Assay Buffer 5x 1ml 1615 TopoGEN
TG4032 5x Topoisomerase IV Assay Buffer (2-part) 1ml 1785 TopoGEN
TG4040 Topo II Assay Buffer (2 part) 1ml 1785 TopoGEN Dry ice
TG4060 10% SDS 1ml 1615 TopoGEN
TG4065 Sarkosyl 20% 5ml 1785 TopoGEN
TG4037 5x Stop Buffer 1ml 1615 TopoGEN
TG4038 10x Gel Loading Buffer 1ml 1615 TopoGEN
TG4039 10x Proteinase K 0.5ml 1615 TopoGEN
TG4035 20mM ATP 0.5ml 1615 TopoGEN
TG4045 5X SA Gyrase Assay Buffer 1ml 1615 TopoGEN
TG4055 5X Potassium Glutamate 1ml 1615 TopoGEN
TG4090 Topoisomerase II Dilution Buffer 1ml 1615 TopoGEN
TG4095 Topoisomerase I Dilution Buffer 1ml 1615 TopoGEN

ProFoldin 药物靶向酶和底物

发表于由

 

 

ProFoldin成立于2005年，位于美国马萨诸塞州，提供专注于药物发现，蛋白质科学和化学分析的产品和服务：

  • 药物靶酶测定

  • 酶和底物

  • 浓度测量

  • 脂质体和纳米光盘

  • 蛋白质制备

  • 分子结合与分离

ProFoldin的产品和服务在生命科学和药物发现实验室中被的大学，研究机构，生物技术公司，制药研发和研究医院广泛采用。 

 

 ProFoldin 药物靶向酶和底物

人酵素
DNA拓扑异构酶    
  人DNA拓扑异构酶I-用于2500种测定 货号 HTOPI-010
  人DNA拓扑异构酶I-用于10,000次测定 货号 HTOPI-040
DNA聚合酶    
  人DNA聚合酶alpha-用于100次检测 货号 HDPA1-100
  人DNA聚合酶alpha-用于1000次检测 货号 HDPA1-1000
  人类DNA聚合酶α全酶-用于100次测定 货号 HDPA-100
  人DNA聚合酶beta-用于100次检测 货号 HDPB-100
  人DNA聚合酶beta-用于1000次检测 货号 HDPB-1000
激酶    
  人胸苷酸激酶（TMK）-用于500次检测 货号 HTMK-500
聚（ADP-核糖）聚合酶-1（PARP-1）    
  人Poly（ADP-核糖）聚合酶-1-用于100次检测 货号 PARP-100
  人Poly（ADP-核糖）聚合酶-1-用于1000次检测 货号 PARP-1000
RNA聚合酶    
  人线粒体RNA聚合酶-用于100次检测 货号 MRPA-100
       
细菌酶和蛋白质
β-内酰胺酶    
  大肠杆菌 β-内酰胺酶-用于100次检测 货号 LAC-100EC
生物素蛋白连接酶（BirA）    
  大肠杆菌 BirA-用于100次检测 货号 BIRA-100KEC
D-Ala-D-Ala连接酶    
  大肠杆菌 D-Ala-D-Ala连接酶-用于100次检测 货号 DDL-100EC
DNA促旋酶（细菌  DNA拓扑异构酶II ）    
  大肠杆菌 DNA促旋酶-用于100次检测 货号 TOP2-100EC
  大肠杆菌 gyrA-用于100次检测 货号 GYRA-100EC
  大肠杆菌 gyrB-用于100次检测 货号 GYRB-100EC
  金黄色葡萄球菌 DNA促旋酶-用于100次测定 货号 TOP2-100SA
  金黄色葡萄球菌 gyrA-用于100次检测 货号 GYRA-100SA
  金黄色葡萄球菌 gyrB-用于100次检测 货号 GYRB-100SA
  铜绿假单胞菌 DNA促旋酶-用于100次检测 货号 TOP2-100PA
  铜绿假单胞菌 GyrA-用于100次检测 货号 GYRA-100PA
  铜绿假单胞菌 GyrB-用于100次检测 货号 GYRB-100PA
DNA解旋酶    
  大肠杆菌 DNA解旋酶-用于100次检测 货号 DNAB-100EC
  H.流感 DNA解旋酶-用于100次检测 货号 DNAB-100HI
  铜绿假单胞菌 DNA解旋酶-用于100次检测 货号 DNAB-100PA
  肺炎链球菌 DNA解旋酶-用于100次检测 货号 DNAB-100SP
DNA连接酶    
  大肠杆菌 DNA连接酶-用于100次检测 货号 LIG-100EC
DNA聚合酶    
  大肠杆菌 DNA聚合酶α-用于100次检测 货号 DPA-100EC
  肺炎链球菌 DNA聚合酶α-用于100次检测 货号 DPA-100SP
  H.流感 DNA聚合酶α-用于100次检测 货号 DPA-100HI
DNA启动酶    
  大肠杆菌 DNA引发酶-用于100次检测 货号 DNAG-100EC
  H.流感 DNA引发酶-用于100次检测 货号 DNAG-100HI
  肺炎链球菌 DNA引发酶-用于100次检测 货号 DNAG-100SP
  金黄色葡萄球菌 DNA引发酶-用于100次检测 货号 DNAG-100SA
DNA拓扑异构酶I     
  大肠杆菌 DNA拓扑异构酶I-用于100次检测 货号 *-100EC
DNA拓扑异构酶II    
  参见DNA促旋酶    
DNA拓扑异构酶IV（parC-parE复合物）    
  大肠杆菌 DNA拓扑异构酶IV-用于100次检测 货号 TOP4-100EC
  大肠杆菌 parC-用于100次检测 货号 PARC-100EC
  大肠杆菌 parE-用于100次检测 货号 PARE-100EC
  金黄色葡萄球菌 DNA拓扑异构酶IV-用于100次测定 货号 TOP4-100SA
  金黄色葡萄球菌 parC-用于100次测定 货号 PARC-100SA
  金黄色葡萄球菌 parE-用于100次检测 货号 PARE-100SA
  铜绿假单胞菌 DNA拓扑异构酶IV-用于100次测定 货号 TOP4-100PA
  铜绿假单胞菌 parC-用于100次测定 货号 PARC-100PA
  铜绿假单胞菌 parE-用于100次检测 货号 PARE-100PA
  肺炎链球菌 DNA拓扑异构酶IV-用于100次检测 货号 TOP4-100SP
  肺炎链球菌 parC-用于100次检测 货号 PARC-100SP
  肺炎链球菌 parE-用于100次检测 货号 PARE-100SP
DNA结合蛋白    
  大肠杆菌单链DNA结合蛋白-100 µg 货号 S SB-100EC
激酶    
  大肠杆菌 NAD激酶-用于100次检测 货号 NADK-100EC
  大肠杆菌胸苷酸激酶（TMK）-用于100次检测 货号 TMK-100EC
  大肠杆菌 UMP激酶-用于100次检测 货号 UMK-100EC
  大肠杆菌鸟苷酸激酶-用于100次检测 货号 GMK-100EC
  肺炎链球菌鸟苷酸激酶-用于100次检测 货号 GMK-100SP
甲基赤藓糖醇磷酸胞苷基转移酶（IspD）    
  大肠杆菌 IspD-用于100次检测 货号 ISPD-100EC
  铜绿假单胞菌 IspD-用于100次检测 货号 ISPD-100PA
穆拉    
  大肠杆菌 MurA-用于100次检测 货号 MURA-100EC
MurC    
  大肠杆菌 MurC-用于100次检测 货号 MURC-100EC
  铜绿假单胞菌 MurC-用于100次检测 货号 MURC-100PA
  肺炎链球菌 MurC-用于100次检测 货号 MURC-100SP
默德    
  大肠杆菌 MurD-用于100次检测 货号 MURD-100EC
  铜绿假单胞菌 MurD-用于100次检测 货号 MURD-100PA
  金黄色葡萄球菌 MurD-用于100次检测 货号 MURD-100SA
MurE    
  大肠杆菌 MurE-用于100次检测 货号 MURE-100EC
       
墨尔    
  大肠杆菌 MurF-用于100次检测 货号 MURF-100EC
  铜绿假单胞菌 MurF-用于100次检测 货号 MURF-100PA
  金黄色葡萄球菌 MurF-用于100次检测 货号 MURF-100SA
  肺炎链球菌 MurF-用于100次检测 货号 MURF-100SP
烟酸磷酸核糖基转移酶（PncB）    
  金黄色葡萄球菌 PncB-用于100次检测 货号 PNCB-100SA
  肺炎链球菌 PncB-用于100次检测 货号 PNCB-100SP
RNA聚合酶    
  大肠杆菌 RNA聚合酶-用于100次检测 货号 RNAP-100EC
  金黄色葡萄球菌 RNA聚合酶-用于100次测定 货号 RNAP-100SA
  大肠杆菌多核苷酸磷酸化酶-用于100次检测 货号 PNP-100EC
UDP-N-乙酰氨基葡糖焦磷酸化酶（GlmU）    
  大肠杆菌 GlmU – 100个测定小号 货号 GLMU-100EC
       
聚合酶的酶底物和模板
  超螺旋DNA用于DNA弛豫分析 货号 SDNA-050UG
  轻松的DNA用于DNA超螺旋测定 货号 RDNA-050UG
  级联DNA用于DNA弛豫分析 货号 KDNA-100
  缺口DNA用于DNA连接酶测定 货号 NLA100K-DNA
  用于人类DNA聚合酶α测定的DNA模板 货号 HDPA100K-DNA
  用于人类DNA聚合酶beta测定的DNA模板 货号 DPB100K-DNA
  用于人类DNA聚合酶γ测定的DNA模板 货号 DPG100K-DNA
  用于线粒体RNA聚合酶测定的DNA模板 货号 MRPA100K-DNA
  用于大肠杆菌 RNA聚合酶测定的DNA模板 货号 RPA100K-DNA
  金黄色葡萄球菌 RNA聚合酶测定的DNA模板 货号 RPA100KS-DNA
  用于噬菌体T7 RNA聚合酶测定的DNA模板  货号 T7RPA100K-DNA
  用于M-MLV逆转录酶测定的DNA模板 货号 MLV100K-DNA
  HIV逆转录酶测定的DNA模板 货号 HIV100K-DNA
  用于AMV逆转录酶测定的DNA模板 货号 AMV100K-DNA
  用于病毒RNA依赖性RNA聚合酶测定的RNA模板 货号 VRT100K-RNA
  用于大肠杆菌多核苷酸磷酸化酶测定的RNA模板 货号 PNP100K-RNA
  用于大肠杆菌 DNA引发酶测定的DNA模板 货号 EGA100K-DNA
  用于流感嗜血杆菌 DNA引发酶测定的DNA模板 货号 HGA100K-DNA
  用于肺炎链球菌 DNA引发酶测定的DNA模板 货号 PGA100K-DNA
  S的DNA模板。金黄色 DNA引物酶测定 货号 AGA100K-DNA
  MurC底物-UDP-MurNAc 货号 MURC-SUB1
  MurD底物-UDP-MurNAc-L-Ala 货号 MURD-SUB1
  MurE substarte-UDP-MurNAc-二肽 货号 MURE-SUB1
  MurF亚启动-UDP-MurNAc-三肽 货号 MURF-SUB1

 

 

TopoGEN书2020年版来啦~~

发表于由

 

 

美国TopoGEN公司成立于1991年，是一家诊断公司，由一批在拓扑异构酶领域工作和在基础、临床、应用及相关方面研究经验丰富的研究者创建，为以药物开发为基础的拓扑异构酶和机制研发人员提供创新产品和服务。药物筛选试剂盒可以分析拓扑异构酶活性药物，用于癌症化疗和抗菌方面。TopoGEN公司还能提供原核和真核化验拓扑异构酶 (I型，II，IV和旋转酶)检测试剂盒，体外体内诊断均可应用。TopoGEN公司还提供DNA载体、DNA裂解目标、拓扑异构酶抑制剂、拓扑异构酶抗体和相关试剂盒等完整产品线。

上海金畔TopoGEN书2020年版来啦~~

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