组成

状态

容量

(A)抗体固相化 96 孔板（干燥孔板）

洗净后使用

96 wells（8×12）/1 块

(B)瘦素标准溶液（小鼠）（5,000 pg/mL）

稀释后使用

500 μL/1 瓶

(C)缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗瘦素抗体

稀释后使用

200 μL/1 瓶

(E)过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F)显色液（TMB）

即用

12 mL/1 瓶

(H)反应终止液（1M H₂SO₄）※小心轻放

即用

12 mL/1 瓶

( I )浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

动物种类

对象物质

反应性和反应率（％）

Mouse

Leptin

100

α-MSH

―

IFN-γ

―

MCH

―

TNF-α

―

Rat

Leptin

31.5

Human

Lepin

＋

样品

A

B

C

1

3818

846

405

2

3810

856

405

3

3979

842

394

4

4047

851

392

5

4046

856

420

mean

3940

850

403

SD

118

6.18

11.2

CV(%)

3.0

0.73

2.8

测量日/样品

D

E

第０天

5007

1052

第１天

5126

1063

第２天

5069

1027

第３天

5000

1000

mean

5051

1035

SD

59.3

28.0

CV(%)

1.2

2.7

添加量

实测值

回收量

回收率(%)

0.00

994.9

–

–

337

1334.0

339.1

101

1061

2099.0

1104

104

1238

2284.0

1289

104

添加量

实测值

回收量

回收率(%)

0.00

1996

–

–

2731

4741

2745

101

4682

6444

4448

95.0

5462

7717

5721

105

ELISA试剂盒选择指南①②

 1.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin Resistance and Hepatic Steatosis in High-Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes. 2017 Apr;66(4):868-879 

 2.

DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes. Kuroda M, Tominaga A, Nakagawa K, Nishiguchi M, Sebe M, Miyatake Y, Kitamura T, Tsutsumi R, Harada N, Nakaya Y, Sakaue H. PLoS One. 2016 Aug 5;11(8):e0160532.

 3.

Sodium alginate prevents progression of non-alcoholic steatohepatitis and liver carcinogenesis in obese and diabetic mice. Miyazaki T, Shirakami Y, Kubota M, Ideta T, Kochi T, Sakai H, Tanaka T, Moriwaki H, Shimizu M. Oncotarget. 2016 Mar 1;7(9):10448-58.

 4.

Comparison of two Kampo medicines in a diet-induced mouse obesity model. Fengying Gao, Satoru Yokoyama, Makoto Fujimoto, Koichi Tsuneyama, Ikuo Saiki, Yutaka Shimada andYoshihiro Hayakawa. Traditional & Kampo Medicine, Volume 2, Issue 2, pages 60–66, September 2015

 5.

Effect of Keishibukuryogan on Genetic and Dietary Obesity Models. Fengying Gao, Satoru Yokoyama, Makoto Fujimoto, Koichi Tsuneyama, Ikuo Saiki, Yutaka Shimada, and Yoshihiro Hayakawa. Evid Based Complement Alternat Med. 2015; 2015: 801291.

 6.

Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect. Kamohara R, Yamaza H, Tsuchiya T, Komatsu T, Park S, Hayashi H, Chiba T, Mori R, Otabe S, Yamada K, Nagayasu T, Shimokawa I. Exp Gerontol. 2015 Apr;64:46-54.

 7.

Preventive effects of astaxanthin on diethylnitrosamine-induced liver tumorigenesis in C57/BL/KsJ-db/db obese mice. Ohno T, Shimizu M, Shirakami Y, Miyazaki T, Ideta T, Kochi T, Kubota M, Sakai H, Tanaka T, Moriwaki H. Hepatol Res. Jul 2015.

 8.

Sudachitin, a polymethoxylated flavone, improves glucose and lipid metabolism by increasing mitochondrial biogenesis in skeletal muscle. Tsutsumi R, Yoshida T, Nii Y, Okahisa N, Iwata S,Tsukayama M, Hashimoto R, Taniguchi Y, Sakaue H, Hosaka T, Shuto E, Sakai T. 

Nutrition & Metabolism, 11:32, Jul 2014.

 9.

Type 2 diabetic conditions in Otsuka Long-Evans Tokushima Fatty rats are ameliorated by 5-aminolevulinic acid. Sato T, Yasuzawa T, Uesaka A, Izumi Y, Kamiya A, Tsuchiya K, Kobayashi Y, Kuwahata M, Kido Y. Nutr Res. Vol.34(6), p544-551, Jun 2014.

10.

Inhibitory Effects of Japanese Herbal Medicines Sho-saiko-to and Juzen-taiho-to on Nonalcoholic Steatohepatitis in Mice. Takahashi Y, Soejima Y, Kumagai A, Watanabe M, Uozaki H, Fukusato T. PLoS One. 2014 Jan 22;9(1):e87279.

11.

Down-Regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin via Leptin Signaling in Diabetic/Obese KK-A y Mice. Beppu F., Hosokawa M., Yim M-J., Shinoda T., Miyashita K. Lipids, Vol.48(5), p449-455, May 2013.

12.

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita. Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012.

13.

Effects of Gametophytes of Ecklonia Kurome on the Levels of Glucose and Triacylglycerol in db/db, Prediabetic C57BL/6J and IFN-γ KO Mice. F. Dwiranti., M. Hiraoka., T. Taguchi., Y. Konishi., M. Tominaga., A. Tominaga. Int J B 64 iomed Sci, Vol.8, No.1, Mar 2012.

14.

Overexpression of FoxO1 in the Hypothalamus and Pancreas Causes Obesity and Glucose Intolerance. H.-J. Kim., M. Kobayashi., T. Sasaki., O. Kikuchi., K. Amano., T. Kitazumi., Y.-S. Lee., H. Yokota-Hashimoto., V. Y. Susanti., Y. Ido Kitamura., J. Nakae., and T. Kitamura. Endocrinology, Vol.153, No.2, p659-671, Feb 2012.

15.

Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome. M. Fujimoto., K. Tsuneyama., T. Fujimoto., C. Selmid., 

M. E. Gershwin., Y. Shimada. Digestive and Liver Disease, 2012.

16.

Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice. Y. Yamashita., M. Okabe., M. Natsume., H. Ashida. Archives of 

Biochemistry and Biophysics, 2012.

17.

Preventive Effects of Curcumin on the Development of Azoxymethane-Induced Colonic Preneoplastic Lesions in Male C57BL/KsJ-db/db Obese Mice. M. Kubota., M. Shimizu., H. Sakai., Y. Yasuda., D. Terakura., A. Baba., T. Ohno., H. Tsurumi., T. Tanaka., H. Moriwaki. Nutrition and Cancer, Vol. 64(1), 2012.

18.

Caffeic Acid Phenethyl Ester Suppresses the Production of Adipocytokines, Leptin, Tumor Necrosis Factor -Alpha and Resistin, during Differentiation to Adipocytes in 3T3-L1 Cells. S, Juman., N, Yasui., H, Okuda., A,Ueda., H, Negishi., T, Miki. and K, Ikeda. Biological and Pharmaceutical Bulletin Vol. 34 (2011) , No.4, 490.

19.

Mate Tea(Ilex paraguariensis)Promotes Satiety and Body Weight Lowering in Mice:Involvement of Glucagon-Like Peptide-1. G, M, E, Hussein., H, Matsuda., S, Nakamura., M, Hamao., T, Akiyama., K, Tamura., and M, Yoshikawa. Biol.Pharm.Bull. Vol.34(12), p1849-1855, 2011.