抗体种类

  多克隆抗体

抗原

  Iba1的合成肽（C端序列相同）

免疫动物

  兔子

交叉性

  人、小鼠、大鼠、其他^※

浓度

  0.5-0.7 mg/mL

应用

  免疫组织染色（冰冻切片）：1︰500-1,000

  免疫细胞染色： 1︰500-1,000

储存条件

  -20℃冷冻保存（干冰运输）

抗原来源动物

  大鼠

物种：小鼠

部位：小脑

样品：冰冻切片

抗体浓度：1︰1,000

物种：小鼠

部位：伏隔核

样品：超薄切片

抗体浓度：1︰500

<数据提供>

东京理科大学生命生物科学科 萩原老师、山崎先生

物种：小鼠

部位：脊髓

样品：冰冻切片

抗体浓度：1︰500

<数据提供>

东京理科大学生命生物科学科 萩原老师、山崎先生

物种：小鼠

部位：视网膜

抗体浓度：1︰1,000

<数据提供>

Prof. Lieve Moons, KU Leuven, Belgium.

物种：大鼠

部位：大脑皮层

样品：冰冻切片

抗体浓度：1︰1,000

<数据提供>

日本国立精神·神经医疗研究中心

佐柳老师、真锅老师、一户老师、高坂老师

产品详情

产品等级 

  小胶质细胞标记物抗体——Anti   Iba1抗体

免疫化学用

   抗Iba1,   重组兔源单克隆抗体（6A4）

免疫化学用

   抗Iba1，兔（免疫细胞化学用）

免疫化学用

   抗Iba1，兔（石蜡切片用）

免疫化学用

   抗Iba1，兔 生物素结合

免疫化学用

   抗Iba1，兔源（结合488绿色荧光）（Prototype）

免疫化学用

   抗Iba1, 兔源, 结合SPICA Dye™ 568

免疫化学用

   抗   Iba1，兔源，SPICA Dye™ 594偶联

免疫化学用

   Anti-Iba1、红色荧光染料（635）结合

免疫化学用

   抗Iba1，山羊多克隆抗体

免疫化学用

   鼠源Iba1抗体，无标签，单克隆抗体（NCNP24）

免疫化学用

   抗Iba1, 兔源单克隆抗体（NCNP27）

免疫化学用

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Lane

1. Iba1 protein (10 ng)

2. 大鼠小胶质细胞 (10 µg)

3. 大鼠神经元 (10 µg)

4. 大鼠大脑皮层 (150 µg)

■ 实验条件

SDS-PAGE：5.5 % stacking gel, 12.5 % running gel, 100V

Blocking：3 % 脱脂奶粉/TBS ，1 h，室温

一抗：抗Iba1抗体(1/1000) ，3 % 脱脂奶粉/TTBS，Overnight，4℃

二抗：过氧化物酶标记抗兔IgG（1/5000），3 % 脱脂奶粉/TTBS，1 h，室温

Protocol for IHC-P(Anti Iba, Rabbit)

Protocol for IHC-Fr(Anti Iba Rabbit)

产品编号

产品名称

2017年发表文献引用

019-19741

Anti Iba1, Rabbit 

(for   Immunocytochemistry)

[1]

Tomov N, Surchev L. Punctate   Staining as Indirect Evidence for Microglial Ramification[J]. Acta   morphologica et anthropologica, 24: 1-2.<链接>

[2]

Barua S, Chung J I, Kim A Y, et   al. Jak kinase 3 signaling in microgliogenesis from the spinal nestin+   progenitors in both development and response to injury[J]. Neuroreport, 2017,   28(14): 929.<链接>

[3]

Dilution A S. Supplementary   Table S2. Antibodies used in immunohistochemical, flatmount and   immunoblotting studies[J].

<链接>

[4]

Su W S, Wu C H, Chen S F, et al.   Low-intensity pulsed ultrasound improves behavioral and histological outcomes   after experimental traumatic brain injury[J]. Scientific Reports, 2017, 7(1):   15524.

<链接>

[5]

Ebneter A, Kokona D, Jovanovic   J, et al. Dramatic Effect of Oral CSF-1R Kinase Inhibitor on Retinal   Microglia Revealed by In Vivo Scanning Laser Ophthalmoscopy[J]. Translational   vision science & technology, 2017, 6(2): 10-10.<链接>

016-20001

Anti Iba1, Rabbit

 (for   Western Blotting)

[1]

Zhang X, Wang D, Pan H, et al.   Enhanced expression of markers for astrocytes in the brain of a line of   GFAP-TK transgenic mice[J]. Frontiers in neuroscience, 2017, 11.

<链接>

[2]

Barua S, Chung J I, Kim A Y, et   al. Jak kinase 3 signaling in microgliogenesis from the spinal nestin+   progenitors in both development and response to injury[J]. Neuroreport, 2017,   28(14): 929.<链接>

[3]

Peters D G, Purnell C J, Haaf M   P, et al. Dietary lipophilic iron accelerates regional brain iron-load in   C57BL6 mice[J]. Brain Structure and Function, 2017: 1-18.<链接>

[4]

Edwards A, Szklarczyk A,   Ottenheimer D, et al. Matrix metalloproteinase activity stimulates N-cadherin   shedding and the soluble N-cadherin ectodomain promotes classical microglial   activation[J]. Journal of neuroinflammation, 2017, 14(1): 56.<链接>

[5]

Roche S L, Ruiz‐Lopez A M,   Moloney J N, et al. Microglial‐induced Müller cell gliosis is attenuated by   progesterone in a mouse model of retinitis pigmentosa[J]. Glia,   2017.

<链接>