Capralogics CI0163说明书

 

Capralogics CI0163说明书

anti-Adiponectin Receptor AdipoR1 N-terminus

抗脂联素受体AdipoR1 N末端

SKU: CI0163

 

 

 

IH石蜡:ADPR1 N末端人肝脏(上图)。IH石蜡:ADPR1 N末端IH人肌肉(下图)。

产品规格

量: 

200微克

寄主物种: 

山羊

抗原: 

25个氨基酸(aa)合成肽SSHKGSVVAQGNGAPASNREADTVE对应于AdipoR1的N-末端结构域的aa 2-26

反应种类: 

高度保守,提示多种物种

格式/偶联物/净化: 

肽亲和纯化

缓冲液和配方: 

亲和纯化抗体@ 1mg / ml,10 mM KHPO4,140 mM NaCl,BSA @ 1 mg / ml和0.1%sodium azide

存储: 

等分并在-20°C冷冻。避免冻融循环。

克隆: 

多克隆

应用

Western Blotting: 

1:1000

IHC: 

1:250(石蜡切片)

评论: 

脂联素受体1(AdipoR1)是一种完整的膜蛋白,具有七个跨膜结构域,具有内部N末端和外部C末端。AdipoR1在物种中高度保守,并由许多细胞表达。骨骼肌是AdipoR1的丰富来源。肥胖人体脂肪组织(AT)中AdipoR1的表达下降。体重减轻导致AT中AdipoR1增加。

参考文献: 

Kadowaki T,Yamauchi T,Kubota N,Hara K,Ueki K,Tobe K.胰岛素抵抗,糖尿病和代谢综合征中的脂联素和脂联素受体。J Clin Invest。2006年7月; 116(7):1784-92。 
Rasmussen MS,Lihn AS,Pedersen SB,Bruun JM,Rasmussen M,Richelsen B.人脂肪组织中的脂联素受体:肥胖,体重减轻和脂肪库的影响。肥胖(银泉)。2006年1月; 14(1):28-35。 
Staiger H,Kaltenbach S,Staiger K,Stefan N,Fritsche A,Guirguis A,Peterfi C,Weisser M,Machicao F,Stumvoll M,Haring HU。脂联素受体mRNA在人骨骼肌细胞中的表达与葡萄糖和脂质代谢的体内参数有关。糖尿病。2004年9月; 53(9):2195-201。 
Yamauchi T,et al。克隆介导抗糖尿病代谢作用的脂联素受体。性质。2003年6月12日; 423(6941):762-9。  

 

 

 

货号 

品名

规格

美金价

品牌

AC0201

PACS-1

100ul

295

capralogics

AC0202

DAPR

250ug

295

capralogics

AC0203

TMX4 (monoclonal)

100 ug

295

capralogics

AC0206

TMX-KLH

250 ug

295

capralogics

AS0010

Normal Alpaca Serum (10ml)

10ml

125

capralogics

CC0208

HMG-CoA (HMGCR)

250 ug

295

capralogics

CI0110

anti-human TRAIL-R2

200 ug

299

capralogics

CI0112

anti-human TRAIL-R3

200 ug

299

capralogics

CI0114

anti-human CCR5

200 ug

299

capralogics

CI0115

anti-human CXCR4

200 ug

299

capralogics

CI0116

anti-mouse CXCR4

200 ug

299

capralogics

CI0117

anti-human CCR4

200 ug

299

capralogics

CI0118

anti-human CCR2

200 ug

299

capralogics

CI0119

anti-human CCR3

200 ug

299

capralogics

CI0120

anti-human CCR8 N-terminal

200 ug

299

capralogics

CI0121

anti-human CCR8 2nd External Cellular Domain

100 ug

299

capralogics

CI0122

anti-mouse CCR4 N-Terminal

200 ug

299

capralogics

CI0123

anti-mouse CCR8

200 ug

299

capralogics

CI0124

anti-human CCR9

200 ug

299

capralogics

CI0125

anti-mouse CCR9

200 ug

299

capralogics

CI0126

anti-human CCR10

200 ug

299

capralogics

CI0127

anti-mouse CCR10

200 ug

299

capralogics

CI0128

anti-human CCR11

200 ug

299

capralogics

CI0129

anti-human CCR6

200 ug

299

capralogics

CI0130

anti-human CCR D6

200 ug

299

capralogics

CI0131

anti-mouse CCR7

200 ug

299

capralogics

CI0132

anti-mouse CCR D6

200 ug

299

capralogics

CI0133

anti-human TLR2

200 ug

299

capralogics

CI0134

anti-human TLR5

200 ug

299

capralogics

CI0136

anti-human p73L

200 ug

295

capralogics

CI0137

anti-human p51A

200 ug

295

capralogics

CI0138

anti-human pp36 T-cell adaptor

100 ug

195

capralogics

CI0139

anti-human IL-21 C-terminus

200 ug

299

capralogics

CI0140

anti-human IL-21 N-terminus

200 ug

299

capralogics

CI0141

anti-mouse IL-21 C-terminus

200 ug

299

capralogics

CI0142

anti-mouse IL-21 N-terminus

200 ug

299

capralogics

CI0143

anti-human IL-22 C-terminus

200 ug

299

capralogics

CI0144

anti-human IL-22 N-terminus

200 ug

299

capralogics

CI0145

anti-mouse IL-22 N-terminus

200 ug

299

capralogics

CI0146

MAPKK 7

100 ug

299

capralogics

CI0147

anti-human CCR1

200 ug

299

capralogics

CI0148

anti-mouse IL-21 Receptor

200 ug

299

capralogics

CI0149

anti-human IL-21 Receptor

200 ug

299

capralogics

CI0150

anti-human IL-22R-alpha-2

200 ug

299

capralogics

CI0151

anti-mouse IL-28RA

200 ug

299

capralogics

CI0152

anti-human IL-22 Receptor

200 ug

299

capralogics

CI0154

anti-mouse CCR3

200 ug

299

capralogics

CI0155

anti-mouse IL-22 N-terminus

100 ul

299

capralogics

CI0156

anti-mouse IL-21 Receptor

100 ul

299

capralogics

CI0157

anti-human IL-23 Receptor

200 ug

299

capralogics

CI0158

anti-mouse TLR9

200 ug

299

capralogics

CI0159

anti-human TLR4

200 ug

299

capralogics

CI0160

anti-human TLR8

200 ug

299

capralogics

CI0161

anti-human TLR9

200 ug

299

capralogics

CI0162

anti-human TLR10

200 ug

299

capralogics

CI0163

anti-Adiponectin Receptor AdipoR1 N-terminus

200 ug

295

capralogics

CI0164

anti-Adiponectin Receptor AdipoR1 C-terminus

200 ug

295

capralogics

CI0165

anti-Adiponectin Receptor AdipoR2 N-terminus

200 ug

295

capralogics

CI0166

anti-Adiponectin Receptor AdipoR2 C-terminus

200 ug

295

capralogics

CI0167

anti-human IL-21

200 ug

299

capralogics

CI0168

Human (and Mouse) ADPR1 C-terminus aa317-340

200 ug

295

capralogics

CI0169

human ADPR2 C-terminus aa362-380

200 ug

295

capralogics

CI0170

human ADPR2 C-terminus2 aa321-342

200 ug

295

capralogics

CI0171

Beta Chemokine Receptor D6 (human) Biotin Conjugated IgG

100 ug

299

capralogics

CI0172

CCR9 (Mouse) Biotin conjugated IgG

100 ug

299

capralogics

CI0173

Angiopoietin 4 (mouse)

200 ug

299

capralogics

CI0174

Angiopoietin 4 (human)

200 ug

299

capralogics

CI0175

Adiponutrin 3 (mouse)

200 ug

299

capralogics

CI0176

Adiponectin Receptor 1 (ADPR1) (mouse) N-terminus

200 ug

299

capralogics

FC0101

GCN2

100ug

265

capralogics

FC0102

DNA-PKcs

100ug

265

capralogics

FC0103

LIMK-1

100ug

265

capralogics

FC0104

LIMK-2

100ug

265

capralogics

FC0105

PERK

100ug

265

capralogics

FC0106

IRE1a

100ug

265

capralogics

FC0107

TIE2

100ug

265

capralogics

FC0108

MAP3K10

100ug

265

capralogics

FC0109

Trk-A

100ug

265

capralogics

FC0110

FADK

100ug

265

capralogics

FC0111

GSK-3 beta

100ug

265

capralogics

FC0112

SGK1

100ug

265

capralogics

FC0113

SGK2

100ug

265

capralogics

FC0114

SGK3

100ug

265

capralogics

FC0115

FADK2

100ug

265

capralogics

FC0116

DCK

100ug

265

capralogics

FC0117

MLCK

100ug

265

capralogics

FC0118

MAPK1

100ug

265

capralogics

FC0119

MAPK8

100ug

265

capralogics

FC0120

MAPK9

100ug

265

capralogics

FC0121

MAPK10

100ug

265

capralogics

FC0122

MAPK11

100ug

265

capralogics

FC0123

MAPK12

100ug

265

capralogics

FC0124

MAPK13

100ug

265

capralogics

FC0125

anti-STAT6

100ug

265

capralogics

FC0126

MAP2K1

100ug

265

capralogics

FC0127

MAPK2

100ug

265

capralogics

FC0128

MAPK3

100ug

265

capralogics

FC0129

MAPK4

100ug

265

capralogics

FC0130

MAPK6

100ug

265

capralogics

GA0050

Normal Goat IgG Ammonium Sulfate Cut

20 mg

50

capralogics

GG0010

Normal Goat IgG Protein G Purified

10 mg

50

capralogics

GS0125

Normal Goat Serum (125 ml)

125 ml

75

capralogics

GS0250

Normal Goat Serum (250 ml)

250 ml

125

capralogics

GS0500

Normal Goat Serum (500 ml)

500 ml

175

capralogics

GS1000

Normal Goat Serum (1000 ml)

1000 ml

300

capralogics

LS0010

Normal Llama Serum (10 ml)

10 ml

125

capralogics

P00002

anti-Histone 1

250 ug

200

capralogics

P00004

anti-Histone 2B

250 ug

200

capralogics

P00006

anti-Histone 3

250 ug

200

capralogics

P00008

anti-Z-DNA

250 ug$200

 

capralogics

P00013

anti-BrdU

250 ug

225

capralogics

P00364

anti-Endothelin A (ET(A))

250 ug

200

capralogics

P00366

anti-Endothelin B (ET(B))

250 ug

200

capralogics

P00367

anti-EGFr

250 ug

200

capralogics

P00368

anti-EpoR

250 ug

200

capralogics

P00390

anti-Glucose Transporter 1

250 ug

200

capralogics

P00391

Glucose Transporter 1-FITC Conjugate

100ug

295

capralogics

P00702

anti-Angiotensin II type 1 (AT(1)) receptor

250 ug

200

capralogics

P00704

anti-5-methyl cytosine

250 ug

200

capralogics

P00801

anti-LAT1

250 ug

275

capralogics

P00802

anti-PCB

1 mg

250

capralogics

P00803

anti-IgY

100 ul

175

capralogics

P00804

anti-IgY-HRP

100 ul

195

capralogics

P00805

anti-IgY-FITC

100 ul

195

capralogics

P00806

anti-IgY-AP

100 ul

195

capralogics

P00807

anti-IgY-Biotin

100 ul

195

capralogics

P00808

anti-Rabbit IgG

1 mg

175

capralogics

P00809

anti-mouse IgG

1 mg

175

capralogics

P00810

anti-human IgG

1 mg

175

capralogics

P00811

anti-Goat IgG

1 mg

175

capralogics

P00812

anti-bovine serum albumin

1 mg

250

capralogics

P00813

anti-PP1

1 mg

250

capralogics

P00814

anti-PP1 Alpha

1 mg

250

capralogics

P00815

anti-PP1 Beta

1 mg

250

capralogics

P00816

anti-PP1 Gamma 1

1 mg

250

capralogics

P00817

anti-PP1 Gamma 2

1 mg

250

capralogics

P00818

anti-PP1 PP2A/C

1 mg

250

capralogics

P00819

anti-PP1 PP2B

1 mg

250

capralogics

P00820

anti-Elongin A

1 mg

250

capralogics

P00821

anti-Elongin B

1 mg

250

capralogics

P00822

Elongin C

1 mg

250

capralogics

P00823

anti-T4

1 mg

250

capralogics

P00824

anti-TSH

1 mg

250

capralogics

P00825

anti-Beta-TSH

1 mg

250

capralogics

P00826

anti-TSH Sandwich Pair – 2 IgY Antibodies

2 x 1 mg

250

capralogics

P00827

anti-TBG

1 mg

250

capralogics

P00828

anti-hCG

1 mg

250

capralogics

P00829

anti-hCG beta

1 mg

250

capralogics

P00830

anti-hCG sandwich pair

2 x 1 mg

400

capralogics

P00831

anti-hCG HRP conjugate

100 ul

250

capralogics

P00832

anti-hCG beta HRP conjugate

100 ul

250

capralogics

P00851

anti-actin

250 ug

295

capralogics

P00852

anti-myosin II

150 ug

295

capralogics

P00853

anti-FGF2

150 ug

295

capralogics

PIP007

anti-Histone Core

250 ug

200

capralogics

PL0101

Rabbit anti-Llama

1 mg

100

capralogics

PL0102

Rabbit anti-Llama HRP Conjugate

250 ug

195

capralogics

PL0103

Rabbit anti-Llama Alkaline Phosphatase

250 ug

195

capralogics

PL0104

Rabbit anti-Llama Biotin Conjugate

250 ug

195

capralogics

PL0105

Rabbit anti-Llama FITC Conjugate

250 ug

195

capralogics

PL0106

Goat anti-Llama

1 mg

100

capralogics

PL0107

Goat anti-Llama HRP Conjugate

250 ug

195

capralogics

PL0108

Goat anti-Llama Alkaline Phosphatase

250 ug

195

capralogics

PL0109

Goat anti-Llama Biotin Conjugate

250 ug

195

capralogics

PL0110

Goat anti-Llama FITC Conjugate

250 ug

195

capralogics

PL0111

Rabbit anti-llama Heavy Chain

1 mg

195

capralogics

PL0112

Rabbit anti-llama Light Chain

1 mg

195

capralogics

PL0113

Rabbit anti-llama Heavy Chain HRP

250 ug

195

capralogics

PL0114

Rabbit anti-llama Light Chain HRP

250 ug

195

capralogics

PL0115

Rabbit anti-llama Heavy Chain Biotin Conjugate

250 ug

195

capralogics

PL0116

Rabbit anti-llama Light Chain Biotin Conjugate

250 ug

195

capralogics

PL0117

Rabbit anti-llama Heavy Chain Alkaline Phosphatase

250 ug

195

capralogics

PL0118

Rabbit anti-llama Light Chain Alkaline Phosphatase

250 ug

195

capralogics

PL0119

Rabbit anti-Llama IgG Heavy Chain FITC Conjugate

250 ug

195

capralogics

PL0120

Rabbit anti-Llama IgG Light Chain FITC Conjugate

250 ug

195

capralogics

PL0121

Llama anti-Mouse IgG

250 ug

199

capralogics

PL0122

Rabbit anti-Alpaca IgG

1 mg

100

capralogics

PL0123

Rabbit anti-llama IgG1

1 mg

100

capralogics

PL0124

Goat anti-Alpaca IgG

1 mg

100

capralogics

RA0050

Normal Rabbit IgG Ammonium Sulfate Cut

50 mg

50

capralogics

RG0010

Normal Rabbit IgG Protein G Purified

10 mg

50

capralogics

RS0025

Normal Rabbit Serum (25 ml)

25 ml

50

capralogics

SA0050

Normal Sheep IgG Ammonium Sulfate Cut

50 mg

50

capralogics

SG0010

Normal Sheep IgG Protein G Purified

10 mg

50

capralogics

SS1000

Normal Sheep Serum (1000 ml)

1000 ml

300

capralogics

 

 

 

 

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reddotbiotech RD-ADIPOR1-Mu说明书

 

Reddot Biotech Inc.目前专注于销售用于制药,实验室,医学和科研用途的试剂和相关产品。我们致力于研究和开发,为客户提供高质量的产品,为任何研究提供准确,可靠和快速的结果。凭借与各大洲公司和研究机构合作的丰富经验,我们为我们提供的产品和服务提供了巨大的价值。

RD-ADIPOR1-MuMouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

Catalog Number

RD-ADIPOR1-Mu

Detection Range

3.125-200ng/mL

Assay Length

1-4.5 hours

Method

sandwich

Sensitivity

1.17ng/mL

 

ACDCR1CGI45PAQR1TESBP1ATESBP1-AProgestin and AdipoQ Receptor Family Member I

窗体顶端

 

 

 

 

 

 

 

 

窗体顶端

 

货号

品名

规格

品牌

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

96T

reddotbiotech

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

48T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

96T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

48T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

96T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

96T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

48T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

96T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

48T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

96T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

48T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

96T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

96T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

96T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

48T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

96T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

48T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

96T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

48T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

96T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

48T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

96T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

48T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

96T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

48T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

96T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

48T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

96T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

48T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

96T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

48T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

 

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7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

Reddot RDR-ADIPOR1-Mu说明书

 

Reddot Biotech Inc.位于加拿大不列颠哥伦比亚省基洛纳,由一支在医疗器械行业拥有超过35年经验的执行团队于2015年成立。我们专注于为市场的客户提供及时的客户服务,可靠的供应和具有性能的产品。我们依靠竞争,创新和快速资源分配来满足客户需求。

Reddot Biotech Inc.目前专注于销售用于制药,实验室,医学和科研用途的试剂和相关产品。我们致力于研究和开发,为客户提供高质量的产品,为任何研究提供准确,可靠和快速的结果。凭借与各大洲公司和研究机构合作的丰富经验,我们为我们提供的产品和服务提供了巨大的价值。

 

 

 

 

RDR-ADIPOR1-MuMouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

小鼠脂联素受体1(ADIPOR1)ELISA试剂盒

目录编号

RDR-ADIPOR1沐

检测范围

3.12-200ng /毫升

分析长度

1-3个小时

方法

sandwich

灵敏度

1.17ng / mL的

存储

所有组件均可在4°C下储存

可以使用

预稀释检测试剂,增强稳定性

 

 

 

 

货号

品名

规格

品牌

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

96T

reddotbiotech

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

48T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

96T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

48T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

96T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

96T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

48T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

96T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

48T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

96T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

48T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

96T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

96T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

96T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

48T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

96T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

48T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

96T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

48T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

96T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

48T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

96T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

48T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

96T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

48T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

96T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

48T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

96T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

48T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

96T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

48T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

 

 

 

传统的ELISA试剂盒与即用型ELISA试剂盒

我们新的“即用型”ELISA试剂盒具有以下特点:

  • 缩短实验时间 
  • 预稀释检测试剂
  • 增强稳定性,允许在4 o C下储存整个试剂盒

这些试剂盒仍然保持我们高水平的质量和灵敏度,以及我们12个月的保质期。

与传统的ELISA试剂盒相比,我们的即用型试剂盒使用起来更容易,更快捷。

提供的试剂和材料

传统的96T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:2
  • 标准:2
  • 标准稀释液:1 x 20mL
  • 检测试剂A:1×120μL(竞争试剂盒1×70μL)
  • 检测试剂B:1×120μL
  • 分析稀释剂A:1×12mL
  • 分析稀释液B:1×12mL
  • TMB底物:1 x 9mL
  • 终止溶液:1 x 6mL
  • 洗涤缓冲液(30倍浓缩液):1×20mL
  • 使用说明书:1

即用型96T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:2
  • 标准:2
  • 标准稀释液:1 x 20mL
  • 检测溶液A:1x12mL
  • 检测溶液B:1x12mL
  • TMB底物:1 x 9mL
  • 终止溶液:1 x 6mL
  • 洗涤缓冲液(30倍浓缩液):1×20mL
  • 使用说明书:1

 

 

传统的48T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:1
  • 标准:1
  • 标准稀释液:1 x 10mL
  • 检测试剂A:1×60μL(竞争试剂盒1×35μL)
  • 检测试剂B:1×60μL
  • 分析稀释液A:1×6mL
  • 分析稀释液B:1×6mL
  • TMB底物:1×4.5mL
  • 终止溶液:1 x 3mL
  • 洗涤缓冲液(30倍浓缩液):1×10mL
  • 使用说明书:1

 

即用型48T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:1
  • 标准:1
  • 标准稀释液:1 x 10mL
  • 检测溶液A:1x6mL
  • 检测溶液B:1x6mL
  • TMB底物:1×4.5mL
  • 终止溶液:1 x 3mL
  • 洗涤缓冲液(30倍浓缩液):1×10mL
  • 使用说明书:1

 

 

 

所需材料但未提供套件

  • 微孔板读数器,450±10nm滤光片。
  • 精密单通道或多通道移液器和一次性吸头。
  • 微量离心管用于稀释。
  • 去离子水或蒸馏水。
  • 用于印迹微量滴定板的吸水纸。
  • 稀释洗涤缓冲液的容器。

存放套件

对于未开封的套件

传统的ELISA试剂盒:所有试剂应根据样品瓶上的标签保存。的TMB底物,洗涤缓冲液(30X浓缩物)终止溶液应在被存储ö Ç 在接收而其他人应在-20 Ô Ç 

即用型ELISA试剂盒:所有试剂应在收到后于o C储存

打开套件

打开试剂盒后,仍需要根据上述储存条件储存剩余的试剂。此外,将未使用的孔返回到含有干燥剂包的铝箔袋中,并沿着拉链密封的整个边缘重新密封。

注意:

有关套件的失效日期,请参阅套件盒上的标签。在此日期之前,所有组件都是稳定的

强烈建议在开封后1个月内使用剩余的试剂。

样品采集和存储

请注意您感兴趣的套件推荐哪些样品类型。如果您有任何疑问或想知道我们是否可以根据您的需求定制套件,请咨询我们

血清

使用血清分离管,让样品在室温下凝固2小时或在4℃下过夜,然后以约1000×g离心20分钟。立即测定新鲜制备的血清,或将样品分装在-20℃或-80℃下备用。避免反复冻/融循环。

等离子体

使用EDTA或肝素作为抗凝血剂收集血浆。在收集后30分钟内,在2-8℃下以1000×g离心样品15分钟。立即去除血浆并进行检测,或将样品保存在-20℃或-80℃的等分试样中以备后用。避免反复冻/融循环。

组织匀浆

组织匀浆的制备将根据组织类型而变化。通常,组织应在冰冷的PBS(0.02mol / L,pH 7.0-7.2)中*冲洗以除去过量的血液并在均质化前称重。将组织切成小块并在冰上用玻璃匀浆器在5-10mL PBS中均化(Micro Tissue Grinders也起作用)。应使用超声波细胞破碎器对所得悬浮液进行超声处理,或进行两次冻融循环以进一步破坏细胞膜。之后,匀浆应以5000×g离心5分钟。立即取出上清液并进行分析,或等分试样并在≤-20℃下储存。

细胞裂解物

根据以下说明,在测定前必须裂解细胞:

  • 应用胰蛋白酶分离贴壁细胞,然后通过离心收集(悬浮细胞可通过直接离心收集)。
  • 在冷PBS中洗涤细胞三次。
  • 在1X PBS中重悬细胞并进行4次超声波处理(或在≤-20℃下冷冻细胞。轻轻混合解冻细胞。重复冻融循环3次。)
  • 在2 – 8℃下以1500×g离心10分钟以除去细胞碎片。细胞培养上清液和其他生物液体 – 以1000×g离心20分钟。立即去除颗粒物并进行分析,或将样品分装在-20℃或-80℃的等分试样中。避免反复冻/融循环。

唾液

使用收集装置或等同物收集唾液。在2-8在1000×g下离心15分钟样品ö C.在≤-20删除在等分试样的微粒,并立即检测,或将ö C.避免反复冷冻/解冻循环。

尿

无菌收集当天的批尿液(中游),直接收集到无菌容器中。离心机以除去颗粒物质,测定立即或等分试样并储存在≤-20 ö C.避免反复冻融。

牛奶

用于以2 10,000×G下离心15分钟的样品- 8 ø C.收集总共3个周期的水性馏分和离心机两次以上。立即测定或将样品分装在-20 o C或-80 o C以备后用。避免反复冻/融循环。

泪液,细胞培养上清液和其他生物液体

以1000×g离心样品20分钟。立即去除颗粒物并进行检测,或将样品保存在-20 o C或-80 o C的等分试样中。避免反复冻/融循环。

注意:                                                

  • 样品在5天内可以被存储在4使用Ô C,否则样品必须储存在-20 Ô C(≤1月)或-80 Ô C(≤2个月),以避免生物活性和/或污染的损失。
  • 样品溶血会影响结果; 不会检测到用作样本的溶血标本。
  • 进行测定时,将样品置于室温下。

试剂准备

*使用前将所有试剂盒组分和样品置于室温(18-25 o C)。

标准 

用标准稀释剂重新配制标准品,在室温下保持10分钟,轻轻摇动(不要起泡)。有关储备溶液中标准浓度的信息,请参阅手册。准备7管含有0.5mL标准稀释剂的试管,并使用稀释的标准品生产双稀释系列。在下次转移之前*混合每个管。

检测试剂A和检测试剂B.

传统的ELISA试剂盒:使用前短暂旋转或离心检测A和检测B溶液。分别用Assay Diluent A和B稀释至工作浓度(1:100)。

即用型ELISA试剂盒:检测溶液A和B的浓度已经正确,无需进一步稀释。

洗液 

用580mL去离子水或蒸馏水稀释20mL洗涤液浓缩物(30×),制备600mL洗涤液(1×)。

TMB底物 

用灭菌的吸头吸取所需剂量的溶液。不要将残留的溶液倒回到小瓶中。

注意:

  • 不要直接在孔中进行连续稀释。
  • 在进行测定后15分钟内准备标准品。不要直接在37 o C 溶解试剂。
  • 检测试剂A和B是粘性溶液,因此慢慢移液它们以减少体积误差。
  • 传统的ELISA试剂盒:根据说明小心地重新构建标准品或使用检测试剂A和B,避免起泡并轻轻混合直至晶体*溶解。为了大限度地减少移液造成的不性,请使用小体积并确保移液器已校准。建议一次移液10μL以上以确保准确性。                 
  • 即用型ELISA试剂盒:重组标准品只能使用一次
  • 重构的标准品,检测试剂A和检测试剂B只能使用一次
  • 如果在洗涤液浓缩物(30×)中形成晶体,则温热至室温并轻轻混合直至晶体*溶解。
  • 试剂制备过程中使用的任何污染水或容器都会影响检测结果。

样品制备

  • Reddot Biotech仅对试剂盒本身负责,而不是对试验过程中消耗的样品负责。实验者应该在开始实验之前总是计算所需的样品总量。
  • 实验者还应该在开始之前预测大致的浓度。如果预期值不在标准曲线范围内,建议确定样品的稀释度。
  • 如果您使用的样品类型未在手册中指出,则应在开始实际实验之前进行初步实验以确保试剂盒的有效性。
  • 建议不要使用化学裂解缓冲液来制备组织或细胞提取样品,因为它可能会由于裂解缓冲液的含量而导致意外的反应和结果。
  • 由于其他来源的抗原与我们试剂盒中使用的抗体之间可能存在错配,因此不建议使用其他制造商生产的天然或重组蛋白,因为我们的试剂盒可能无法识别它们。
  • 我们的试剂盒可能无法检测到细胞培养上清液中的样品,因为它们很容易受到细胞活力,细胞数量和/或取样时间等因素的影响。
  • 建议将未经长期储存的新鲜样品与我们的试剂盒一起使用。如果样品长时间未冷冻保存,可能会发生蛋白质降解和变性,导致结果相互矛盾或不正确。

分析程序

用于夹心ELISA试剂盒

  • 确定稀释标准品,空白样品和样品的孔。为标准准备7口井,为空白准备1口井。每种稀释的标准品(读取试剂制备),空白和样品加入100μL到适当的孔中。用Plate密封剂盖住。在37 o C 孵育2小时。
  • 从每个井中取出液体,不要洗。
  • 向每个孔中加入100μL检测试剂A工作溶液(即用型试剂盒中的检测溶液A)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 吸出溶液,用喷枪,多通道移液器,歧管分配器或自动洗衣机在每个孔中用350μL1×洗涤液洗涤,静置1~2分钟。通过将板轻敲到吸水纸上,*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后,通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转平板并将其吸附在吸水纸上。
  • 向每个孔中加入100μL检测试剂B工作溶液(即用型试剂盒中的检测溶液B)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 如步骤4中所述,重复抽吸/洗涤过程总共5次。
  • 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟(不要超过30分钟)。避光。加入底物溶液后液体会变蓝。
  • 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀,请轻轻敲打板以确保充分混合。
  • 去除板底部的任何水滴和指纹,确认液体表面没有气泡。运行酶标仪,立即进行450nm的测量。

适用于竞争性ELISA试剂盒

  • 确定稀释标准品,空白样品和样品的孔。为标准准备7口井,为空白准备1口井。分别加入50μL标准稀释液(读取试剂制备),空白和样品加入适当的孔中。然后立即向每个孔中加入50μL检测试剂A(即用型试剂盒中的检测溶液A)。轻轻摇动平板(建议使用微孔板振荡器)。用Plate密封剂覆盖。在37 o C 孵育1小时。检测试剂A可能会出现混浊。温热至室温并轻轻混合直至溶液看起来均匀。
  • 吸出溶液,用喷枪,多通道移液器,歧管分配器或自动洗涤器向每个孔中加入350μL1×洗涤液,静置1-2分钟。通过将板轻敲到吸水纸上,*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后,通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转印版并将其贴在吸水纸上。
  • 向每个孔中加入100μL检测试剂B工作溶液(即用型试剂盒中的检测溶液B)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 在步骤2中重复抽吸/洗涤过程总共5次。
  • 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟(不要超过30分钟)。避光。加入底物溶液后液体会变蓝。
  • 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀,请轻轻敲打板以确保充分混合。
  • 去除板底部的任何水滴和指纹,确认液体表面没有气泡。运行酶标仪,立即进行450nm的测量。

注意:

  • 分析准备:为每个实验保留适当数量的孔,并从微孔板中移除额外的孔。剩余的井应重新密封,并保存在-20 o C的传统套件中,4 o C的即用型套件。  
  • 样品或试剂添加:请使用新制备的标准品。小心地将样品加入孔中并轻轻混合以避免起泡。不要触摸井壁。对于该程序中的每个步骤,将试剂或样品添加到测定板的总分配时间不应超过10分钟。这将确保每个移液步骤的相等的经过时间,而不会中断。建议复制所有标准品和标本,但不要求。为避免交叉污染,在添加标准品,样品和试剂之间更换移液器吸头。此外,对每种试剂使用分离的储库。
  • 孵育:为了确保准确的结果,需要在孵育步骤中适当粘附板密封剂。在孵化步骤之间不允许孔长时间未被覆盖。一旦将试剂添加到孔条中,在测定过程中不要让条带干燥。必须控制孵育时间和温度。
  • 洗涤:洗涤程序至关重要。在每个步骤中*去除液体对于试剂盒的良好性能是*的。后一次清洗后,通过抽吸或倾析除去任何残留的清洗液,并去除板底部的任何水滴或指纹。洗涤不足将导致精度差和吸光度读数错误升高。
  • 控制反应时间:观察加入TMB底物后的颜色变化(例如每10分钟观察一次),如果颜色太深,则提前加入终止溶液以避免过强的反应,这将导致吸光度读数不准确。
  • TMB底物  很容易被污染。保护它免受光线和物理污染。 
  • 环境湿度可能对从试剂盒获得的结果产生影响。如果您工厂的湿度低于60%,建议使用加湿器。

 

测试原理

用于夹心ELISA试剂盒

该试剂盒中提供的微量滴定板预先涂有对靶抗原特异的抗体。然后将标准品或样品加入适当的微量滴定板孔中,用对靶抗原特异的生物素缀合的抗体制剂。接下来,将与辣根过氧化物酶(HRP)缀合的抗生物素蛋白加入每个微孔板孔中并孵育。在添加TMB底物溶液后,仅含有靶抗原,生物素缀合的抗体和酶缀合的抗生物素蛋白的孔将显示颜色变化。通过添加硫酸溶液终止酶 – 底物反应,并且在450nm±10nm的波长下通过分光光度法测量颜色变化。然后通过比较样品的OD与标准曲线确定样品中靶抗原的浓度。

适用于竞争性ELISA试剂盒

该测定采用竞争性抑制酶免疫测定技术。将靶抗原特异性的单克隆抗体预先包被在微孔板上。在生物素标记的靶抗原和未标记的靶抗原(标准品或样品)之间启动竞争性抑制反应,其中预涂的抗体对靶抗原具有特异性。温育后,洗去未结合的缀合物。接下来,将与辣根过氧化物酶(HRP)缀合的抗生物素蛋白加入每个微孔板孔中并孵育。结合的HRP缀合物的量是反向正比于样品中的靶抗原的浓度。加入底物溶液后,显色的强度与样品中靶抗原的浓度成比例。

结果计算

用于夹心ELISA试剂盒

平均每个标准品,对照品和样品的重复读数,然后减去平均零标准光密度。通过绘制每个标准品的平均OD和浓度构建标准曲线,并通过图表上的点绘制zui佳拟合曲线,或在log-log图纸上创建标准曲线,其中y轴上的目标抗原浓度和吸光度x轴。还建议使用绘图软件(例如,曲线专家1.30)。如果样品已稀释,则从标准曲线读取的浓度必须乘以稀释倍数。

适用于竞争性ELISA试剂盒

该测定采用竞争性抑制酶免疫测定技术,因此样品中的靶抗原浓度与测定信号强度之间存在负相关。平均每个标准品,对照品和样品的重复读数。在对数 – 对数或半对数图纸上创建标准曲线,在y轴上记录目标抗原浓度,在x轴上记录吸光度。通过标准点绘制zui佳拟合直线,或者可以通过回归分析确定。还建议使用绘图软件(例如,曲线专家1.30)。如果样品已稀释,则从标准曲线读取的浓度必须乘以稀释倍数。

灵敏度

该测定的灵敏度或检测下限(LLD)被定义为可以与零区分的低蛋白质浓度。通过将两个标准偏差加到二十个零标准重复的平均光密度值并计算相应的浓度来确定。

夹心ELISA试剂盒的典型数据

为了使计算更容易,我们绘制标准的OD值(X轴)与标准的已知浓度(Y轴),尽管浓度是自变量,OD值是因变量。然而,标准曲线的OD值可以根据测定性能的条件(例如操作者,移液技术,洗涤技术或温度效应)而变化,建议绘制数据的对数以建立每个测试的标准曲线。有关典型的标准曲线,请参阅套件中包含的手册。

确定所有套件精度的一般方法如下所述。可根据要求提供个人批量套件的规格。

  • 测定内精密度(测定内的度):分别在一个平板上测试3个具有低,中和高水平指数的样品20次。
  • 批间精密度(测定之间的度):在3个不同的板上测试3个具有低,中和高水平的样品的指数,每个板中重复8次。
  • CV(%)= SD /平均值(X)100
    • 分析内:CV <10%
    • Inter-Assay:CV <12%

稳定性

我们的ELISA试剂盒的稳定性取决于活性丧失的速率。在适当的储存条件下,我们的试剂盒的失效率在失效日期内通常低于5%。

为尽量减少外部对我们套件性能的影响,应始终控制操作程序和实验室条件。需要特别注意的因素包括:室温,空气湿度和培养箱温度。还强烈建议整个测定由相同的实验者从开始到结束进行。

分析程序摘要

用于夹心ELISA试剂盒

  • 准备所有试剂,样品和标准品;
  • 每孔加入100μL标准品或样品。在37 o C 孵育2小时;
  • 吸出并加入100μL制备的检测试剂A(即用型试剂盒中的检测溶液A)。在37 o C 孵育1小时;
  • 吸3次洗净;
  • 加入100μL制备的检测试剂B(即用型试剂盒中的检测溶液B)。在37 o C 孵育1小时;
  • 吸取并洗涤5次;
  • 加入90μL底物溶液。在37 o C 孵育15-25分钟;
  • 加入50μL终止液。立即读取450nm。

适用于竞争性ELISA试剂盒

  • 准备所有试剂,样品和标准品;
  • 每孔加入50μL标准品或样品。然后立即加入50μL制备的检测试剂A(即用型试剂盒中的检测溶液A)。摇晃和混合。在37oC孵育1小时;
  • 吸3次洗净;
  • 加入100μL制备的检测试剂B(即用型试剂盒中的检测溶液B)。在37oC孵育1小时;
  • 吸取并洗涤5次;
  • 加入90μL底物溶液。在37oC孵育15-25分钟;
  • 加入50μL终止液。立即读取450nm。

重要的提示

  • 受现有条件和科学技术的限制,不可能对供应商提供的原材料进行全面的鉴定和分析测试。因此,可能存在一些定性和/或技术风险。
  • 终的实验结果将与产品的有效性,终用户的操作技能和实验环境密切相关。请确保有足够的样本可以获得准确的结果。
  • 不同批次的试剂盒在检测范围,灵敏度和显色时间上可能略有不同。请*按照套件中包含的使用说明书进行实验。电子版仅供参考。
  • 不要将试剂从一个试剂盒中混合或替换为另一个试剂盒。仅使用制造商提供的试剂。
  • 在储存和孵育期间保护所有试剂免受强光照射。所有试剂瓶盖应紧密关闭,以防止液体蒸发和微生物污染。
  • 当次打开板时,孔中可能存在雾状物质。它对终的测定结果没有任何影响。在需要之前,不要从储存袋中取出微量滴定板。
  • 试剂准备和装载过程中的程序不正确,以及读板器的参数设置不正确可能导致错误的结果。在450±10nm波长下带宽为10nm或更小且光密度范围为0-3OD或更大的微孔板读数器可用于吸光度测量。请仔细阅读说明书并在实验前调整仪器。
  • 即使是同一个实验者也可能从两个独立的实验中获得不同的结果。为了获得更好的可重复结果,应该控制测定中每个步骤的操作。此外,建议在每批次的一般测定之前进行初步实验。
  • 每个套件都经过了几次严格的质量控制测试。但是,由于某些意外的运输条件或不同的实验室设备,终用户的结果可能与我们的内部数据不一致。不同批次的试剂盒之间的测定内差异也可能来自上述因素。
  • 来自不同制造商的相同产品的套件可能会产生不同的结果,因为我们没有将我们的产品与其他制造商进行比较。
  • 该试剂盒中的标准品以及抗体制备中使用的抗原通常是重组蛋白质。不同表达的序列,表达系统和/或纯化方法可用于制备重组蛋白。在我们的试剂盒中,抗体和抗体对的筛选技术也存在差异。因此,我们无法保证我们的试剂盒能够检测其他公司生产的重组蛋白。我们不建议使用Reddot Biotech ELISA试剂盒检测其他重组蛋白。
  • 有效期:12个月。
  • 说明书也适用于48T套件,但48T套件中的所有试剂都减少了一半。

预防

提供与该试剂盒一起使用的终止溶液是酸溶液。使用此试剂时,请佩戴眼睛,手,脸和衣物。

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。

 

Reddot RDR-ADIPOR1-Hu说明书

 

Reddot Biotech Inc.位于加拿大不列颠哥伦比亚省基洛纳,由一支在医疗器械行业拥有超过35年经验的执行团队于2015年成立。我们专注于为市场的客户提供及时的客户服务,可靠的供应和具有性能的产品。我们依靠竞争,创新和快速资源分配来满足客户需求。

Reddot Biotech Inc.目前专注于销售用于制药,实验室,医学和科研用途的试剂和相关产品。我们致力于研究和开发,为客户提供高质量的产品,为任何研究提供准确,可靠和快速的结果。凭借与各大洲公司和研究机构合作的丰富经验,我们为我们提供的产品和服务提供了巨大的价值。

 

 

 

 

RDR-ADIPOR1-HuHuman Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

人类脂联素受体1(ADIPOR1)ELISA试剂盒

目录编号

RDR-ADIPOR1胡

检测范围

0.781-50ng / mL的

分析长度

1-3个小时

方法

sandwich

灵敏度

0.25ng / mL的

存储

所有组件均可在4°C下储存

可以使用

预稀释检测试剂,增强稳定性

 

 

 

 

货号

品名

规格

品牌

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

96T

reddotbiotech

RD-ACE2-Ra

Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit

48T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

96T

reddotbiotech

RD-ACVAB-Hu

Human Activin AB (ACVAB) ELISA Kit

48T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Hu

Human Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

96T

reddotbiotech

RD-ACVA-Mu

Mouse Activin A (ACVA) ELISA Kit

48T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

96T

reddotbiotech

RD-ADIPOR1-Ra

Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Hu

Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

96T

reddotbiotech

RD-ADIPOR2-Ra

Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit

48T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-b

Bovine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-c

Canine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ch

Chicken Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Hu

Human Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Mu

Mouse Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-p

Porcine Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Ra

Rat Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

96T

reddotbiotech

RD-ADP-Rb

Rabbit Adiponectin (ADP) ELISA Kit

48T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Hu

Human Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Mu

Mouse Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Hu

Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

96T

reddotbiotech

RD-AGTR1-Ra

Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Hu

Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

96T

reddotbiotech

RD-AGTR2-Ra

Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit

48T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

96T

reddotbiotech

RD-AGT-Ra

Rat Angiotensinogen (AGT) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Hu

Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Mu

Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

96T

reddotbiotech

RD-ALCAM-Ra

Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-c

Canine Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Hu

Human Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Mu

Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

96T

reddotbiotech

RD-Ang1-7-Ra

Rat Angiotensin 1-7 (Ang1-7) ELISA Kit

48T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

96T

reddotbiotech

RD-Ang1-9-Hu

Human Angiotensin 1-9 (Ang1-9) ELISA Kit

48T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-c

Canine Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Hu

Human Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-b

Bovine Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Hu

Human Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

96T

reddotbiotech

RD-AngIII-Hu

Human Angiotensin III (AngIII) ELISA Kit

48T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Mu

Mouse Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Ra

Rat Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

96T

reddotbiotech

RD-AngII-Rb

Rabbit Angiotensin II (AngII) ELISA Kit

48T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Mu

Mouse Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

96T

reddotbiotech

RD-AngI-Ra

Rat Angiotensin I (AngI) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Hu

Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Mu

Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

96T

reddotbiotech

RD-ATP4a-Ra

Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

48T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

96T

reddotbiotech

RD-Bcl11A-Hu

Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit

48T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-b

Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Hu

Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

96T

reddotbiotech

RD-Bcl2L2-Ra

Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit

48T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

96T

reddotbiotech

RD-Bcl2L-Mu

Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Mu

Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

96T

reddotbiotech

RD-Bcl2-Ra

Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit

48T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

96T

reddotbiotech

RD-Bcl9-Mu

Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit

48T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-c

Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ch

Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Gu

Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Hu

Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Mu

Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-p

Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Ra

Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

96T

reddotbiotech

RD-BDNF-Rb

Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit

48T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Hu

Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Mu

Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

96T

reddotbiotech

RD-BLC1-Ra

Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit

48T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

96T

reddotbiotech

RD-BLK-Hu

Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit

48T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-b

Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ch

Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Hu

Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Mu

Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-p

Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Ra

Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

96T

reddotbiotech

RD-BMP2-Rb

Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit

48T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-b

Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-c

Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ch

Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-g

Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Hu

Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Mu

Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-p

Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Ra

Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

96T

reddotbiotech

RD-BMP4-Rb

Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Hu

Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Mu

Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

96T

reddotbiotech

RD-BMPR1A-Ra

Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit

48T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

96T

reddotbiotech

RD-C1QTNF1-Hu

Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit

48T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

96T

reddotbiotech

RD-C1QTNF3-Hu

Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit

48T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

96T

reddotbiotech

RD-C1QTNF9-Hu

Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit

48T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

96T

reddotbiotech

RD-cAMP-Ge

General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit

48T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

96T

reddotbiotech

RD-CSF2Rb-Hu

Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

48T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-b

Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Hu

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Mu

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-p

Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Ra

Rat Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

96T

reddotbiotech

RD-CTGF-Rb

Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit

48T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

96T

reddotbiotech

RD-CTLA4-Hu

Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit

48T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

96T

reddotbiotech

RD-EDN1-c

Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c

48T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Gu

Guinea pig Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Hu

Human Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Mu

Mouse Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-p

Porcine Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Ra

Rat Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

96T

reddotbiotech

RD-EDN1-Rb

Rabbit Endothelin 1 (EDN1) ELISA Kit

48T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Hu

Human Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

96T

reddotbiotech

RD-EDN2-Ra

Rat Endothelin 2 (EDN2) ELISA Kit

48T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Hu

Human Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Mu

Mouse Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-p

Porcine Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Hu

Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Mu

Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

96T

reddotbiotech

RD-EGFR2-Ra

Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit

48T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

96T

reddotbiotech

RD-EGF-Ra

Rat Epidermal Growth Factor (EGF) ELISA Kit

48T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Hu

Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Mu

Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

96T

reddotbiotech

RD-EGFR-Ra

Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-b

Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Hu

Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Mu

Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

96T

reddotbiotech

RD-EG-VEGF-Ra

Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

48T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Hu

Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Mu

Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

96T

reddotbiotech

RD-FGF10-Ra

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit

48T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Hu

Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Mu

Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

96T

reddotbiotech

RD-FGF11-Ra

Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit

48T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Hu

Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Mu

Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

96T

reddotbiotech

RD-FGF12-Ra

Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit

48T

reddotbiotech

 

 

 

传统的ELISA试剂盒与即用型ELISA试剂盒

我们新的“即用型”ELISA试剂盒具有以下特点:

  • 缩短实验时间 
  • 预稀释检测试剂
  • 增强稳定性,允许在4 o C下储存整个试剂盒

这些试剂盒仍然保持我们高水平的质量和灵敏度,以及我们12个月的保质期。

与传统的ELISA试剂盒相比,我们的即用型试剂盒使用起来更容易,更快捷。

提供的试剂和材料

传统的96T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:2
  • 标准:2
  • 标准稀释液:1 x 20mL
  • 检测试剂A:1×120μL(竞争试剂盒1×70μL)
  • 检测试剂B:1×120μL
  • 分析稀释剂A:1×12mL
  • 分析稀释液B:1×12mL
  • TMB底物:1 x 9mL
  • 终止溶液:1 x 6mL
  • 洗涤缓冲液(30倍浓缩液):1×20mL
  • 使用说明书:1

即用型96T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:2
  • 标准:2
  • 标准稀释液:1 x 20mL
  • 检测溶液A:1x12mL
  • 检测溶液B:1x12mL
  • TMB底物:1 x 9mL
  • 终止溶液:1 x 6mL
  • 洗涤缓冲液(30倍浓缩液):1×20mL
  • 使用说明书:1

 

 

传统的48T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:1
  • 标准:1
  • 标准稀释液:1 x 10mL
  • 检测试剂A:1×60μL(竞争试剂盒1×35μL)
  • 检测试剂B:1×60μL
  • 分析稀释液A:1×6mL
  • 分析稀释液B:1×6mL
  • TMB底物:1×4.5mL
  • 终止溶液:1 x 3mL
  • 洗涤缓冲液(30倍浓缩液):1×10mL
  • 使用说明书:1

 

即用型48T ELISA试剂盒

  • 预涂,即用型96孔带钢板:1
  • 96孔密封板:1
  • 标准:1
  • 标准稀释液:1 x 10mL
  • 检测溶液A:1x6mL
  • 检测溶液B:1x6mL
  • TMB底物:1×4.5mL
  • 终止溶液:1 x 3mL
  • 洗涤缓冲液(30倍浓缩液):1×10mL
  • 使用说明书:1

 

 

 

所需材料但未提供套件

  • 微孔板读数器,450±10nm滤光片。
  • 精密单通道或多通道移液器和一次性吸头。
  • 微量离心管用于稀释。
  • 去离子水或蒸馏水。
  • 用于印迹微量滴定板的吸水纸。
  • 稀释洗涤缓冲液的容器。

存放套件

对于未开封的套件

传统的ELISA试剂盒:所有试剂应根据样品瓶上的标签保存。的TMB底物,洗涤缓冲液(30X浓缩物)终止溶液应在被存储ö Ç 在接收而其他人应在-20 Ô Ç 

即用型ELISA试剂盒:所有试剂应在收到后于o C储存

打开套件

打开试剂盒后,仍需要根据上述储存条件储存剩余的试剂。此外,将未使用的孔返回到含有干燥剂包的铝箔袋中,并沿着拉链密封的整个边缘重新密封。

注意:

有关套件的失效日期,请参阅套件盒上的标签。在此日期之前,所有组件都是稳定的

强烈建议在开封后1个月内使用剩余的试剂。

样品采集和存储

请注意您感兴趣的套件推荐哪些样品类型。如果您有任何疑问或想知道我们是否可以根据您的需求定制套件,请咨询我们

血清

使用血清分离管,让样品在室温下凝固2小时或在4℃下过夜,然后以约1000×g离心20分钟。立即测定新鲜制备的血清,或将样品分装在-20℃或-80℃下备用。避免反复冻/融循环。

等离子体

使用EDTA或肝素作为抗凝血剂收集血浆。在收集后30分钟内,在2-8℃下以1000×g离心样品15分钟。立即去除血浆并进行检测,或将样品保存在-20℃或-80℃的等分试样中以备后用。避免反复冻/融循环。

组织匀浆

组织匀浆的制备将根据组织类型而变化。通常,组织应在冰冷的PBS(0.02mol / L,pH 7.0-7.2)中*冲洗以除去过量的血液并在均质化前称重。将组织切成小块并在冰上用玻璃匀浆器在5-10mL PBS中均化(Micro Tissue Grinders也起作用)。应使用超声波细胞破碎器对所得悬浮液进行超声处理,或进行两次冻融循环以进一步破坏细胞膜。之后,匀浆应以5000×g离心5分钟。立即取出上清液并进行分析,或等分试样并在≤-20℃下储存。

细胞裂解物

根据以下说明,在测定前必须裂解细胞:

  • 应用胰蛋白酶分离贴壁细胞,然后通过离心收集(悬浮细胞可通过直接离心收集)。
  • 在冷PBS中洗涤细胞三次。
  • 在1X PBS中重悬细胞并进行4次超声波处理(或在≤-20℃下冷冻细胞。轻轻混合解冻细胞。重复冻融循环3次。)
  • 在2 – 8℃下以1500×g离心10分钟以除去细胞碎片。细胞培养上清液和其他生物液体 – 以1000×g离心20分钟。立即去除颗粒物并进行分析,或将样品分装在-20℃或-80℃的等分试样中。避免反复冻/融循环。

唾液

使用收集装置或等同物收集唾液。在2-8在1000×g下离心15分钟样品ö C.在≤-20删除在等分试样的微粒,并立即检测,或将ö C.避免反复冷冻/解冻循环。

尿

无菌收集当天的批尿液(中游),直接收集到无菌容器中。离心机以除去颗粒物质,测定立即或等分试样并储存在≤-20 ö C.避免反复冻融。

牛奶

用于以2 10,000×G下离心15分钟的样品- 8 ø C.收集总共3个周期的水性馏分和离心机两次以上。立即测定或将样品分装在-20 o C或-80 o C以备后用。避免反复冻/融循环。

泪液,细胞培养上清液和其他生物液体

以1000×g离心样品20分钟。立即去除颗粒物并进行检测,或将样品保存在-20 o C或-80 o C的等分试样中。避免反复冻/融循环。

注意:                                                

  • 样品在5天内可以被存储在4使用Ô C,否则样品必须储存在-20 Ô C(≤1月)或-80 Ô C(≤2个月),以避免生物活性和/或污染的损失。
  • 样品溶血会影响结果; 不会检测到用作样本的溶血标本。
  • 进行测定时,将样品置于室温下。

试剂准备

*使用前将所有试剂盒组分和样品置于室温(18-25 o C)。

标准 

用标准稀释剂重新配制标准品,在室温下保持10分钟,轻轻摇动(不要起泡)。有关储备溶液中标准浓度的信息,请参阅手册。准备7管含有0.5mL标准稀释剂的试管,并使用稀释的标准品生产双稀释系列。在下次转移之前*混合每个管。

检测试剂A和检测试剂B.

传统的ELISA试剂盒:使用前短暂旋转或离心检测A和检测B溶液。分别用Assay Diluent A和B稀释至工作浓度(1:100)。

即用型ELISA试剂盒:检测溶液A和B的浓度已经正确,无需进一步稀释。

洗液 

用580mL去离子水或蒸馏水稀释20mL洗涤液浓缩物(30×),制备600mL洗涤液(1×)。

TMB底物 

用灭菌的吸头吸取所需剂量的溶液。不要将残留的溶液倒回到小瓶中。

注意:

  • 不要直接在孔中进行连续稀释。
  • 在进行测定后15分钟内准备标准品。不要直接在37 o C 溶解试剂。
  • 检测试剂A和B是粘性溶液,因此慢慢移液它们以减少体积误差。
  • 传统的ELISA试剂盒:根据说明小心地重新构建标准品或使用检测试剂A和B,避免起泡并轻轻混合直至晶体*溶解。为了大限度地减少移液造成的不性,请使用小体积并确保移液器已校准。建议一次移液10μL以上以确保准确性。                 
  • 即用型ELISA试剂盒:重组标准品只能使用一次
  • 重构的标准品,检测试剂A和检测试剂B只能使用一次
  • 如果在洗涤液浓缩物(30×)中形成晶体,则温热至室温并轻轻混合直至晶体*溶解。
  • 试剂制备过程中使用的任何污染水或容器都会影响检测结果。

样品制备

  • Reddot Biotech仅对试剂盒本身负责,而不是对试验过程中消耗的样品负责。实验者应该在开始实验之前总是计算所需的样品总量。
  • 实验者还应该在开始之前预测大致的浓度。如果预期值不在标准曲线范围内,建议确定样品的稀释度。
  • 如果您使用的样品类型未在手册中指出,则应在开始实际实验之前进行初步实验以确保试剂盒的有效性。
  • 建议不要使用化学裂解缓冲液来制备组织或细胞提取样品,因为它可能会由于裂解缓冲液的含量而导致意外的反应和结果。
  • 由于其他来源的抗原与我们试剂盒中使用的抗体之间可能存在错配,因此不建议使用其他制造商生产的天然或重组蛋白,因为我们的试剂盒可能无法识别它们。
  • 我们的试剂盒可能无法检测到细胞培养上清液中的样品,因为它们很容易受到细胞活力,细胞数量和/或取样时间等因素的影响。
  • 建议将未经长期储存的新鲜样品与我们的试剂盒一起使用。如果样品长时间未冷冻保存,可能会发生蛋白质降解和变性,导致结果相互矛盾或不正确。

分析程序

用于夹心ELISA试剂盒

  • 确定稀释标准品,空白样品和样品的孔。为标准准备7口井,为空白准备1口井。每种稀释的标准品(读取试剂制备),空白和样品加入100μL到适当的孔中。用Plate密封剂盖住。在37 o C 孵育2小时。
  • 从每个井中取出液体,不要洗。
  • 向每个孔中加入100μL检测试剂A工作溶液(即用型试剂盒中的检测溶液A)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 吸出溶液,用喷枪,多通道移液器,歧管分配器或自动洗衣机在每个孔中用350μL1×洗涤液洗涤,静置1~2分钟。通过将板轻敲到吸水纸上,*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后,通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转平板并将其吸附在吸水纸上。
  • 向每个孔中加入100μL检测试剂B工作溶液(即用型试剂盒中的检测溶液B)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 如步骤4中所述,重复抽吸/洗涤过程总共5次。
  • 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟(不要超过30分钟)。避光。加入底物溶液后液体会变蓝。
  • 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀,请轻轻敲打板以确保充分混合。
  • 去除板底部的任何水滴和指纹,确认液体表面没有气泡。运行酶标仪,立即进行450nm的测量。

适用于竞争性ELISA试剂盒

  • 确定稀释标准品,空白样品和样品的孔。为标准准备7口井,为空白准备1口井。分别加入50μL标准稀释液(读取试剂制备),空白和样品加入适当的孔中。然后立即向每个孔中加入50μL检测试剂A(即用型试剂盒中的检测溶液A)。轻轻摇动平板(建议使用微孔板振荡器)。用Plate密封剂覆盖。在37 o C 孵育1小时。检测试剂A可能会出现混浊。温热至室温并轻轻混合直至溶液看起来均匀。
  • 吸出溶液,用喷枪,多通道移液器,歧管分配器或自动洗涤器向每个孔中加入350μL1×洗涤液,静置1-2分钟。通过将板轻敲到吸水纸上,*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后,通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转印版并将其贴在吸水纸上。
  • 向每个孔中加入100μL检测试剂B工作溶液(即用型试剂盒中的检测溶液B)。用Plate密封剂覆盖后,在37 o C 孵育1小时。
  • 在步骤2中重复抽吸/洗涤过程总共5次。
  • 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟(不要超过30分钟)。避光。加入底物溶液后液体会变蓝。
  • 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀,请轻轻敲打板以确保充分混合。
  • 去除板底部的任何水滴和指纹,确认液体表面没有气泡。运行酶标仪,立即进行450nm的测量。

注意:

  • 分析准备:为每个实验保留适当数量的孔,并从微孔板中移除额外的孔。剩余的井应重新密封,并保存在-20 o C的传统套件中,4 o C的即用型套件。  
  • 样品或试剂添加:请使用新制备的标准品。小心地将样品加入孔中并轻轻混合以避免起泡。不要触摸井壁。对于该程序中的每个步骤,将试剂或样品添加到测定板的总分配时间不应超过10分钟。这将确保每个移液步骤的相等的经过时间,而不会中断。建议复制所有标准品和标本,但不要求。为避免交叉污染,在添加标准品,样品和试剂之间更换移液器吸头。此外,对每种试剂使用分离的储库。
  • 孵育:为了确保准确的结果,需要在孵育步骤中适当粘附板密封剂。在孵化步骤之间不允许孔长时间未被覆盖。一旦将试剂添加到孔条中,在测定过程中不要让条带干燥。必须控制孵育时间和温度。
  • 洗涤:洗涤程序至关重要。在每个步骤中*去除液体对于试剂盒的良好性能是*的。后一次清洗后,通过抽吸或倾析除去任何残留的清洗液,并去除板底部的任何水滴或指纹。洗涤不足将导致精度差和吸光度读数错误升高。
  • 控制反应时间:观察加入TMB底物后的颜色变化(例如每10分钟观察一次),如果颜色太深,则提前加入终止溶液以避免过强的反应,这将导致吸光度读数不准确。
  • TMB底物  很容易被污染。保护它免受光线和物理污染。 
  • 环境湿度可能对从试剂盒获得的结果产生影响。如果您工厂的湿度低于60%,建议使用加湿器。

 

测试原理

用于夹心ELISA试剂盒

该试剂盒中提供的微量滴定板预先涂有对靶抗原特异的抗体。然后将标准品或样品加入适当的微量滴定板孔中,用对靶抗原特异的生物素缀合的抗体制剂。接下来,将与辣根过氧化物酶(HRP)缀合的抗生物素蛋白加入每个微孔板孔中并孵育。在添加TMB底物溶液后,仅含有靶抗原,生物素缀合的抗体和酶缀合的抗生物素蛋白的孔将显示颜色变化。通过添加硫酸溶液终止酶 – 底物反应,并且在450nm±10nm的波长下通过分光光度法测量颜色变化。然后通过比较样品的OD与标准曲线确定样品中靶抗原的浓度。

适用于竞争性ELISA试剂盒

该测定采用竞争性抑制酶免疫测定技术。将靶抗原特异性的单克隆抗体预先包被在微孔板上。在生物素标记的靶抗原和未标记的靶抗原(标准品或样品)之间启动竞争性抑制反应,其中预涂的抗体对靶抗原具有特异性。温育后,洗去未结合的缀合物。接下来,将与辣根过氧化物酶(HRP)缀合的抗生物素蛋白加入每个微孔板孔中并孵育。结合的HRP缀合物的量是反向正比于样品中的靶抗原的浓度。加入底物溶液后,显色的强度与样品中靶抗原的浓度成比例。

结果计算

用于夹心ELISA试剂盒

平均每个标准品,对照品和样品的重复读数,然后减去平均零标准光密度。通过绘制每个标准品的平均OD和浓度构建标准曲线,并通过图表上的点绘制zui佳拟合曲线,或在log-log图纸上创建标准曲线,其中y轴上的目标抗原浓度和吸光度x轴。还建议使用绘图软件(例如,曲线专家1.30)。如果样品已稀释,则从标准曲线读取的浓度必须乘以稀释倍数。

适用于竞争性ELISA试剂盒

该测定采用竞争性抑制酶免疫测定技术,因此样品中的靶抗原浓度与测定信号强度之间存在负相关。平均每个标准品,对照品和样品的重复读数。在对数 – 对数或半对数图纸上创建标准曲线,在y轴上记录目标抗原浓度,在x轴上记录吸光度。通过标准点绘制zui佳拟合直线,或者可以通过回归分析确定。还建议使用绘图软件(例如,曲线专家1.30)。如果样品已稀释,则从标准曲线读取的浓度必须乘以稀释倍数。

灵敏度

该测定的灵敏度或检测下限(LLD)被定义为可以与零区分的低蛋白质浓度。通过将两个标准偏差加到二十个零标准重复的平均光密度值并计算相应的浓度来确定。

夹心ELISA试剂盒的典型数据

为了使计算更容易,我们绘制标准的OD值(X轴)与标准的已知浓度(Y轴),尽管浓度是自变量,OD值是因变量。然而,标准曲线的OD值可以根据测定性能的条件(例如操作者,移液技术,洗涤技术或温度效应)而变化,建议绘制数据的对数以建立每个测试的标准曲线。有关典型的标准曲线,请参阅套件中包含的手册。

确定所有套件精度的一般方法如下所述。可根据要求提供个人批量套件的规格。

  • 测定内精密度(测定内的度):分别在一个平板上测试3个具有低,中和高水平指数的样品20次。
  • 批间精密度(测定之间的度):在3个不同的板上测试3个具有低,中和高水平的样品的指数,每个板中重复8次。
  • CV(%)= SD /平均值(X)100
    • 分析内:CV <10%
    • Inter-Assay:CV <12%

稳定性

我们的ELISA试剂盒的稳定性取决于活性丧失的速率。在适当的储存条件下,我们的试剂盒的失效率在失效日期内通常低于5%。

为尽量减少外部对我们套件性能的影响,应始终控制操作程序和实验室条件。需要特别注意的因素包括:室温,空气湿度和培养箱温度。还强烈建议整个测定由相同的实验者从开始到结束进行。

分析程序摘要

用于夹心ELISA试剂盒

  • 准备所有试剂,样品和标准品;
  • 每孔加入100μL标准品或样品。在37 o C 孵育2小时;
  • 吸出并加入100μL制备的检测试剂A(即用型试剂盒中的检测溶液A)。在37 o C 孵育1小时;
  • 吸3次洗净;
  • 加入100μL制备的检测试剂B(即用型试剂盒中的检测溶液B)。在37 o C 孵育1小时;
  • 吸取并洗涤5次;
  • 加入90μL底物溶液。在37 o C 孵育15-25分钟;
  • 加入50μL终止液。立即读取450nm。

适用于竞争性ELISA试剂盒

  • 准备所有试剂,样品和标准品;
  • 每孔加入50μL标准品或样品。然后立即加入50μL制备的检测试剂A(即用型试剂盒中的检测溶液A)。摇晃和混合。在37oC孵育1小时;
  • 吸3次洗净;
  • 加入100μL制备的检测试剂B(即用型试剂盒中的检测溶液B)。在37oC孵育1小时;
  • 吸取并洗涤5次;
  • 加入90μL底物溶液。在37oC孵育15-25分钟;
  • 加入50μL终止液。立即读取450nm。

重要的提示

  • 受现有条件和科学技术的限制,不可能对供应商提供的原材料进行全面的鉴定和分析测试。因此,可能存在一些定性和/或技术风险。
  • 终的实验结果将与产品的有效性,终用户的操作技能和实验环境密切相关。请确保有足够的样本可以获得准确的结果。
  • 不同批次的试剂盒在检测范围,灵敏度和显色时间上可能略有不同。请*按照套件中包含的使用说明书进行实验。电子版仅供参考。
  • 不要将试剂从一个试剂盒中混合或替换为另一个试剂盒。仅使用制造商提供的试剂。
  • 在储存和孵育期间保护所有试剂免受强光照射。所有试剂瓶盖应紧密关闭,以防止液体蒸发和微生物污染。
  • 当次打开板时,孔中可能存在雾状物质。它对终的测定结果没有任何影响。在需要之前,不要从储存袋中取出微量滴定板。
  • 试剂准备和装载过程中的程序不正确,以及读板器的参数设置不正确可能导致错误的结果。在450±10nm波长下带宽为10nm或更小且光密度范围为0-3OD或更大的微孔板读数器可用于吸光度测量。请仔细阅读说明书并在实验前调整仪器。
  • 即使是同一个实验者也可能从两个独立的实验中获得不同的结果。为了获得更好的可重复结果,应该控制测定中每个步骤的操作。此外,建议在每批次的一般测定之前进行初步实验。
  • 每个套件都经过了几次严格的质量控制测试。但是,由于某些意外的运输条件或不同的实验室设备,终用户的结果可能与我们的内部数据不一致。不同批次的试剂盒之间的测定内差异也可能来自上述因素。
  • 来自不同制造商的相同产品的套件可能会产生不同的结果,因为我们没有将我们的产品与其他制造商进行比较。
  • 该试剂盒中的标准品以及抗体制备中使用的抗原通常是重组蛋白质。不同表达的序列,表达系统和/或纯化方法可用于制备重组蛋白。在我们的试剂盒中,抗体和抗体对的筛选技术也存在差异。因此,我们无法保证我们的试剂盒能够检测其他公司生产的重组蛋白。我们不建议使用Reddot Biotech ELISA试剂盒检测其他重组蛋白。
  • 有效期:12个月。
  • 说明书也适用于48T套件,但48T套件中的所有试剂都减少了一半。

预防

提供与该试剂盒一起使用的终止溶液是酸溶液。使用此试剂时,请佩戴眼睛,手,脸和衣物。

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等知药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发,欢迎合作。