组分

包装

PROTEOSTAT^® Aggresome Detection Reagent

10 μL

Hoechst 33342 Nuclear Stain

50 μL

Proteasome Inhibitor (MG-132)

120 nM

10×Assay Buffer

25 mL

1.

A fast and specific method to screen for intracellular amyloid inhibitors using bacterial model systems: S. Navarro, et al.; Eur. J. Med. Chem. (2015), Application(s): Confocal microscopy, Abstract;

2.

Amyloidogenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals: Y. Kamada, et al.; Biochim. Biophys. Acta 1850, 1107 (2015), Application(s): Microscopy, Abstract;

Conophylline protects cells in cellular models of neurodegenerative diseases by inducing mammalian target of rapamycin (mTOR)-independent autophagy: Y. Sasazawa, et al.; J. Biol. Chem. 290, 6168 (2015), Abstract;

3.

Decreased proteasomal function accelerates cigarette smoke-induced pulmonary emphysema in mice: Y. Yamada, et al.; Lab. Invest. 95, 625 (2015), Application(s):Aggresome detection by fluorescence microscopy in fibroblasts, Abstract;

4.

Defective autophagy is a key feature of cerebral cavernous malformations: S. Marchi, et al.; EMBO Mol. Med. 7, 1403 (2015), Application(s): Aggresome detection in aggregated proteins and aggresome‐like inclusion bodies in fixed and permeabilized samples,Abstract; Full Text

5.

Fibril growth and seeding capacity play key roles in α-synuclein-mediated apoptotic cell death: A.L. Mahul-Mellier, et al.; Cell Death Differ. 22, 2107 (2015), Abstract;

6.

In vitro administration of gold nanoparticles functionalized with MUC-1 protein fragment generates anticancer vaccine response via macrophage activation and polarization mechanism: T. Mocan, et al.; J. Cancer 6, 583 (2015), Application(s): Aggresome detection by fluorescence microscopy in peritoneal macrophages, Abstract; Full Text

7.

Intensified autophagy compromises the efficacy of radiotherapy against prostate cancer: M.I. Koukourakis, et al.; Biochem. Biophys. Res. Commun. 461, 268 (2015),Application(s): Fluorescence microscopy , Abstract;

Mevalonate pathway regulates cell size homeostasis and proteostasis through autophagy: T.P. Miettinen, et al.; Cell Rep. 13, 2610 (2015), Application(s): Flow cytometry analysis of protein aggregation using Jurkat, U2OS, Kc167 and HUVEC cells, Abstract;

8.

MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib: S. Jagannathan, et al.; Leukemia 29, 727 (2015), Application(s): Detection of protein aggregates by fluorescence microscopy in multiple myeloma cell lines, Abstract; Full Text

9.

Molecular chaperone GRP78 enhances aggresome delivery to autophagosomes to promote drug resistance in multiple myeloma: M.A. Abdel Malek, et al.; Oncotarget 6, 3098 (2015), Application(s): Confocal Microscopy, Abstract; Full Text

10.

Monitoring of dipeptidyl peptidase-IV (DPP-IV) activity in patients with mucopolysaccharidoses types I and II on enzyme replacement therapy – Results of a pilot study: K. Hetmanczyk, et al.; Clin. Biochem. (2015), Application(s): Plasma DPP-IV enzyme assay, Abstract;

11.

Pressure overload-induced cardiac dysfunction in aged male adiponectin knockout mice is associated with autophagy deficiency: J.W. Jahng, et al.; Endocrinology 156, 1667 (2015),Abstract;

Protein kinase C-dependent growth-associated protein 43 phosphorylation regulates gephyrin aggregation at developing GABAergic synapses: C.Y. Wang, et al.; Mol. Cell. Biol.35, 1712 (2015), Abstract;

Schwann cells contribute to neurodegeneration in transthyretin amyloidosis: T. Murakami, et al.; J. Neurochem. 134, 66 (2015), Abstract;

12.

Cationic polystyrene nanospheres induce autophagic cell death through the induction of endoplasmic reticulum stress: H.W. Chiu, et al.; Nanoscale 7, 736 (2014), Abstract;

Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen: S.M. Usmani, et al.; Nat. Commun. 5, 3508 (2014), Application: Amyloid detection in semen,Abstract;

13.

Distinct patterns of HSP30 and HSP70 degradation in Xenopus laevis A6 cells recovering from thermal stress: S. Khan, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 168, 1 (2014), Application(s): Detection of aggresomes in Xenopus laevis cells using fluorescence microscopy, Abstract;

Dynein function and protein clearance changes in tumor cells induced by a kunitz-type molecule, amblyomin-x: M.T. Pacheco, et al.; PLoS One 9, e111907 (2014), Application(s):Detection of aggresomes by flow cytometry, Abstract; Full Text

14.

Higher vulnerability and stress sensitivity of neuronal precursor cells carrying an alpha-synuclein gene triplication: A. Flierl, et al.; PLoS One 9, e112413 (2014), Application(s):Detection of protein aggregates by fluorescence microscopy and flow cytometry in neuronal precursor cells, Abstract; Full Text

15.

Human stefin B role in cell's response to misfolded proteins and autophagy: M. Polajnar, et al.; PLoS One 9, e102500 (2014), Application(s): Detection of protein aggregates in primary astrocytes, Abstract; Full Text

16.

Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells: E. Wolmarans, et al.; Cell Mol. Biol. Lett. 19, 98 (2014), Abstract;

17.

Preconditioning stimulus of proteasome inhibitor enhances aggresome formation and autophagy in differentiated SH-SY5Y cells: Y. Bang, et al.; Neurosci. Lett. 566, 263 (2014),Abstract;

18.

 Protein deubiquitination during oocyte maturation influences sperm function during fertilisation, antipolyspermy defense and embryo development: Y.J. Yi, et al.; Reprod. Fertil. Dev. (2014), Application(s): Detection of protein aggregates in oocytes, Abstract;

19.

Protein expression pattern of PAWP in bull spermatozoa is associated with sperm quality and fertility following artificial insemination: C.E. Kennedy, et al.; Mol. Reprod. Dev. 81, 436 (2014), Abstract;

Serine/threonine kinase 16 and MAL2 regulate constitutive secretion of soluble cargo in hepatic cells: J.G. In, et al.; Biochem. J. 463, 201 (2014), Abstract;

20.

SGTA regulates the cytosolic quality control of hydrophobic substrates: L. Wunderley, et al.; J. Cell. Sci. 127, 4728 (2014), Application(s): Dual staining with ProteoStat® dye, Abstract;Full Text

21.

T he small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget 5, 6252 (2014), Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay, Abstract; Full Text

22.

Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules: M.U. Cheema, et al.; Physiol. Rep. 1, e00064 (2013), Application(s): Labeling of kidney homogenates, labeled particles sorted by flow cytometry and identification by LC-MS/MS ,Abstract; Full Text

23.

Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death: P. Magnaghi, et al.; Nat. Chem. Biol. 9, 548 (2013), Application(s): Detection of aggresomes in human colon carcinoma HCT116 cells using fluorescence microscopy, Abstract;

24.

Environmental stresses induce misfolded protein aggregation in plant cells in a microtubule-dependent manner: Y. Nakajima, et al.; Int. J. Mol. Sci. 14, 7771 (2013),Application(s): Detection of aggresomes using fluorescence microscopy, Abstract; Full Text

25.

In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-β-estradiol analogue in breast adenocarcinoma cells: D.S. Nkandeu, et al.; Cell. Biochem. Funct. 31, 566 (2013), Abstract;

Increased generation of cyclopentenone prostaglandins after brain ischemia and their role in aggregation of ubiquitinated proteins in neurons: H. Liu, et al.; Neurotox. Res. 24, 191 (2013), Abstract;

26.

Macrolide antibiotics block autophagy flux and sensitize to bortezomib via endoplasmic reticulum stress-mediated CHOP induction in myeloma cells: S. Moriya, et al.; Int. J. Oncol.42, 1541 (2013), Application(s): Detection of aggresomes using flow cytometry, Abstract;Full Text

27.

Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2: Y. Maejima, et al.; Nat. Med. 19, 1478 (2013), Application(s): Detection of aggresomes in mouse heart sections using fluorescence microscopy, Abstract; Full Text

28.

N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions: B. Jeric, et al.; Biochim. Biophys. Acta 1833, 2254 (2013), Application(s):Detection of aggresomes using fluorescence microscopy, Abstract;

29.

The ubiquitin proteasome system regulates the stability and activity of the glucose sensor glucokinase in pancreatic beta cells: A. Hofmeister-Brix, et al.; Biochem. J. 456, 173 (2013),Abstract; Full Text

30.

VCP Phosphorylation-Dependent Interaction Partners Prevent Apoptosis in Helicobacter pylori-Infected Gastric Epithelial Cells: C.C. Yu, et al.; PLoS One 8, e55724 (2013),Application(s): Aggresome detection in AGS human gastric epithelial cells, Abstract; Full Text

31.

Zerumbone, an electrophilic sesquiterpene, induces cellular proteo-stress leading to activation of ubiquitin-proteasome system and autophagy: K. Ohnishi, et al.; BBRC 430, 616 (2013), Application(s): Aggresome detection in mouse hepatocytes, Abstract;

32.

Autophagy in idiopathic pulmonary fibrosis: A.S. Patel, et al.; PLoS One 7, e41394 (2012),Application(s): Detection of aggresomes in lung tissue sections using fluorescence microscopy, Abstract; Full Text

33.

Decreased proteasomal activity causes age-related phenotypes and promotes the development of metabolic abnormalities: U. Tomaru, et al.; Am. J. Pathol. 180, 963 (2012),Abstract;

34.

Mutations in the area composita protein αT-catenin are associated with arrhythmogenic right ventricular cardiomyopathy: J. van Hengel, et al.; Eur. Heart J. 34, 201 (2012),Abstract;

35.

Quantitative analysis of α-synuclein solubility in living cells using split GFP complementation: A. Kothawala, et al.; PLoS One 7, e43505 (2012), Application(s):Aggresome detection in HeLa cells, Abstract; Full Text

36.

Multiple aggregates and aggresomes of C-terminal truncated human αA-crystallins in mammalian cells and protection by αB-crystallin: I. Raju, et al.; PLoS One 6, e19876 (2011), Application(s): Aggresome detection in HeLa cells, Abstract; Full Text

37.

Novel Cell- and Tissue-Based Assays for Detecting Misfolded and Aggregated Protein Accumulation Within Aggresomes and Inclusion Bodies: D. Shen, et al.; Cell Biochem. Biophys. 60, 173 (2011), Abstract; Full Text

38.

Inhibitors of protein aggregation and toxicity: H. Amijee, et al.; Biochem. Soc. Trans. 37, 692 (2009), Abstract; Full Text

39.

Autophagy-mediated clearance of aggresomes is not a universal phenomenon: E. Wong, et al.; Hum. Mol. Genet. 17, 2570 (2008), Abstract; Full Text

40.

Chemical and biological approaches synergize to ameliorate protein-folding diseases: T.W. Mu, et al. ; Cell 134, 769 (2008), Abstract; Full Text

41.

Inhibitors of the proteasome suppress homologous DNA recombination in mammalian cells: Y. Murakawa, et al.; Cancer Res. 67, 8536 (2007), Abstract;

42.

p62SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death: G. Bjørkøy, et al.; J.Cell Biol. 171, 603 (2005), Full Text

43.

Therapeutic effects of cystamine in a murine model of Huntington's disease: A. Dedeoglu, et al.; J. Neurosci. 22, 8942 (2002), Full Text