Takara、Clontech、UElandy Inc（试剂）代理
标签归档：clontech
Clontech
Clontech
简要描述：Clontech，代理，国内代理，上海代理，北京代理，中国代理，代理， PCR产品
详细介绍
产品咨询
Clontech，Clontech代理，国内代理，上海代理，北京代理，中国代理，代理， PCR产品
pRevTRE说明书
Clontech 四环素调控系统 pRevTRE载体
pRevTRE
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VSC0468 | pRevTRE | Clontech | 四环素调控系统 |
产品参数:
Promotor: LTR
Size: 6500
Selectable Marker: Hygromycin
Constitutive: Inducible
Viral/Non-Viral: Retroviral
载体抗性:
氨苄青霉素（Ampicillin）
载体描述:
pRevTRE is a retroviral Tet response vector that expresses a gene of interest from the Tet-response element （TRE）. This vector is derived from pLNCX, a retroviral vector created using elements of Moloney murine leukemia virus （MoMuLV） and Moloney murine sarcoma virus （MoMuSV） as described. The TRE contains seven direct repeats of the tetO operator sequence, upstream of a minimal CMV promoter, which can be bound by the tTA and rtTA transactivators. The 5′ viral LTR controls expression of the transcript that contains Y+ （the extended viral packaging signal） and the hygromycin resistance （Hygr） gene for antibiotic selection in mammalian cells. The TRE is derived from vectors described previously. pRevTRE also includes the E. coli Ampr gene for antibiotic selection in bacteria.
The complete RevTet-Off and RevTet-On Systems also include the control vector pRevTRE-Luc, which was constructed by cloning the firefly luciferase gene into the HindIII/Cla I sites in the MCS of pRevTRE.
质粒图谱:
Clontech AH109说明书
Clontech AH109说明书，AH109说明书，Clontech AH109
AH109
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VNC0491 | AH109 | Clontech | 配套酿酒酵母 |
his section provides detailed phenotypes of the yeast strains included with MATCHMAKER System 3
and MATCHMAKER GAL4 cDNA and Genomic Libraries. For additional information on the growth and
maintenance of yeast, see the YPH, Chapter III. We also recommend Guthrie & Fink’s Guide to Yeast
Genetics and Molecular Biology （1991） and Heslot & Gailardin’s Molecular Biology and Genetic
Engineering of Yeasts （1992）.
A. Yeast Host Strains
The complete genotypes of AH109, Y187, and CG-1945 are provided in Table II. All strains are
gal4
–
and gal80
–
; this prevents interference of native regulatory proteins with the regulatory
elements in the two-hybrid system.
1. Use AH109 as the host strain if you plan to screen an AD/library using HIS3, ADE2, and MEL1.
2. System 3 Users Only: Use Y187 as the host strain if you plan to test for an interaction between
two known proteins using the lacZ reporter only. In addition, use Y187 as a mating partner to
verify protein interactions.
3. Library Users Only: Use CG-1945 as the host strain if you plan to separate DNA/bait and
AD/library plasmids by cycloheximide counterselection. Alternatively, use pGBKT7 to construct your bait. This DNA-BD vector contains a kanamycin resistance marker; therefore,
vectors can be separated in E. coli without cycloheximide counterselection.
Clontech 酵母双杂交系统 pGBKT7说明书
Clontech 酵母双杂交系统 pGBKT7， 酵母双杂交系统 pGBKT7，Clontech pGBKT7
pGBKT7
产品参数:
Mammalian Selection：TRP1 （HIS3 selection）
载体抗性:
卡那霉素（Kanamycin）
说明书:
质粒图谱:
Clontech pBridge酵母三杂交系统说明书
Clontech pBridge酵母三杂交系统说明书
载体描述:
pBridge expresses two proteins: a DNA-binding domain fusion, and an additional protein. pBridge thus allows establishment of three-hybrid systems when used in combination with an activation domain fusion vector and yeast strains from any of Clontech’s GAL4-based two-hybrid systems, including Matchmaker™ Gold. This vector generates a hybrid protein that contains the sequences for the GAL4 DNA-binding domain （DNA-BD） and the sequence cloned into MCS I. The fusion protein is expressed in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences （NLS） that are an intrinsic part of the GAL4 DNA-BD. An additional gene of interest can be cloned into MCS II which is located downstream of an HA epitope and a second NLS. The resulting fusion protein is conditionally expressed from the Met25 promoter in response to methionine levels in the medium; i.e., it is repressed in the presence of 1 mM methionine and expressed in the absence of methionine.
质粒图谱:
酵母单杂交系统Clontech pHis2说明书
酵母单杂交系统 Clontech pHis2说明书
Description
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. It contains a HIS3 nutritional reporter gene, located downstream of a multiple cloning site （MCS） and the minimal promoter of the HIS3 locus （PminHIS3）.Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS and used as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the target sequence. A protein-DNA （or one-hybrid） interaction can be detected by performing the assay in a yeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions drive expression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficient media.
In the absence of activation, the constitutive HIS3 expression from PminHIS3 is very low. During library screening, the leaky expression of HIS3 is controlled by adding 3-ami,2,4-triazole （3-AT） to the medium. The concentration of 3-AT needed to fully suppress leaky HIS3 expression must be determined empirically for each DNA target element.
pHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication sequence （ARS4） and TRP1 nutritional marker for replication and selection in yeast （1, 2）; it contains a Col E1 origin and a kanamycin resistance gene （Kanr） for propagation and selection in E. coli. The centromeric sequence CEN6 ensures proper segregation of the plasmid during cell division in yeast （1, 2）.
Use
To use pHIS2 in a one-hybrid assay, clone one or more copies of a cis-acting DNA target sequence into the MCS. Then introduce the plasmid into competent yeast cells using the transformation protocol in the BD Matchmaker Library Construction & Screening Kits User Manual （PT3529-1）. In contrast to the original BD Matchmaker One-Hybrid System, this reporter vector does not need to be integrated into the yeast genome. Instead, it is maintained as an episome throughout the assay. Inserting your target element may alter the level of background HIS3 expression. Therefore, constructs should be tested for background （leaky） HIS3 expression before you start a one-hybrid analysis. Background growth due to leaky HIS3 expression is controlled by adding 3-AT to the selection medium, as described in the User Manual （PT3529-1）.
Location of Features
-
Multiple cloning sites: 141
-
HIS3 gene: 152811
-
Fragment containing the HIS3 3′ UTR & Termination sequence: 8121446
-
TRP1 gene: 28553529
-
Fragment containing the Col E1 E. coli origin of replication: 41654615
-
Kanamycin-resistance gene: 56054811
-
CEN6/ARS4 sequences: 62545737
Clontech 代理
Clontech 代理
简要描述：Clontech代理，Clontech中国代理，Clontech上海代理，Clontech代理，Clontech上海金畔生物科技有限公司Clontech专业代理，具体产品信息欢迎电询：021-50837765
详细介绍
产品咨询
世界*实验材料供应商 Clontech 正式上海金畔为其中国代理， Clontech 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔一直秉承为中国科研客户带来的产品，的服务， Clontech 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
Clontech 中国代理, Clontech 上海代理， Clontech 北京代理，Clontech 广东代理， Clontech 江苏代理Clontech 湖北代理,Clontech 天津,Clontech 黑龙江代理,Clontech 内蒙古代理,Clontech 吉林代理,Clontech 福建代理, Clontech 江苏代理, Clontech 浙江代理, Clontech 四川代理,
美国Clontech是1984年由华人方瑞贤创办的，一度曾经是众多海外华人的骄傲。因为方瑞贤创办的是全美*家，也是亚裔创办的zui大的分子生物公 司，更重要的是Clontech 建立了生物技术中建立基因文库的模式。在Clontech 被BD收购时已有四百名员工，包括六十五位博士，拥有五十多项，产品已达两千多种，堪称数一、数二的基因库，提供三十多个国家基因实验室或是 学术界做研究之用。该公司有曾于一九九四、一九九五、一九九八及一九九九年被圣荷西及旧金山商业报纸选为一百个成长zui快的私人公司。Clontech公司 一直锐意进取，其开发的产品多次获得全美R&D100金奖。产品范围包括基因识别、基因表达、基因功能研究，大家熟悉的Smart Race，Altas DNA矩阵，SSH消减杂交、MTN膜、多色荧光蛋白载体、MatchMaker酵母双杂交系列也在其中。美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品，其强大的科研实力和完善的质量管理体系（ISO9001）为Clontech产品的良好品质提供了可靠保障。
http://www.clontech.com/
部分产品目录
| Brand | Cat. # | Product | Package Size |
| clontech | 636153 | Human Aorta Poly A+ RNA | 5 ug |
| clontech | 636170 | Human Blood, Peripheral Leukocytes Poly A+ RNA | 5 ug |
| clontech | 636113 | Human Heart Poly A+ RNA | 5 ug |
| clontech | 636171 | Human Heart, Auricle Dextra （right） Poly A+ RNA | 5 ug |
| clontech | 636172 | Human Heart, Auricle Sinistra （left） Poly A+ RNA | 5 ug |
| clontech | 636175 | Human Heart, Pericardium Poly A+ RNA | 5 ug |
| clontech | 636173 | Human Heart, Ventricle （left） Poly A+ RNA | 5 ug |
| clontech | 636174 | Human Heart, Ventricle （right） Poly A+ RNA | 5 ug |
| clontech | 636143 | Human Lymph Node Poly A+ RNA | 5 ug |
| clontech | 636121 | Human Spleen Poly A+ RNA | 5 ug |
| clontech | 639537 | SMARTScribe Reverse Transcriptase | 100 Rxns |
| clontech | 634925 | SMARTer PCR cDNA Synthesis Kit | 10 Rxns |
上海金畔生物科技有限公司