标签归档：ctx

fn-test. EH3989说明书

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fn-test EH3989说明书

Human β-CTx(Beta Crosslaps) ELISA Kit

EH3989

  • β-CTx ELISA Kit

  •  适应种属

  • Human

  •  检测范围

  • 125-8000pg/ml

  •  灵敏度

  • 75pg/ml

  • 产品名称

  • Human β-CTx(Beta Crosslaps) ELISA Kit

  • 货号

  • EH3989

  • 别称

  • β-CTx ELISA Kit

  • 检测方法

  • Competitive ELISA, Coated with Antigen

  • 应用

  • β-CTx ELISA Kit

  • 规格

  • 48T/96T

  • 检测范围

  • 125-8000pg/ml

  • 灵敏度

  • < 75pg/ml

  • 种属

  • Human

  • 标准曲线

  • 存储

  • 2-8 ℃ for 6 months

  • 回收率

  • Matrices listed below were spiked with certain level of β-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of β-CTx in samples.

    Matrix Recovery range(%) Average(%)
    serum(n=5) 92-103 97
    EDTA plasma(n=5) 86-105 94
    heparin plasma(n=5) 85-100 91

  • 线性度

  • The linearity of the kit was assayed by testing samples spiked with appropriate concentration of β-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample 1:2 1:4 1:8
    serum(n=5) 87-104% 91-100% 91-104%
    EDTA plasma(n=5) 89-101% 82-98% 83-100%
    heparin plasma(n=5) 82-99% 80-100% 80-100%

  • 变异系数（%）

  • 批内差：CV<8%

  • 注意事项

  • 仅供科研使用

 

RTC-450

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RTC-450

简要描述：RTC-450Chlorotoxin仪器1mgAlomone

详细介绍

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Native Chlorotoxin (CTX) is a 36-amino acid peptide, originally isolated from the venom of the giant yellow Israeli scorpion Leiurus quinquestriatus hebraeus. Primary amino acid sequence shows considerable homology to a class of short insectotoxins.1
 
Initially, CTX has been demonstrated as an irreversible inhibitor of small conductance Cl– channels in colonic epithelial cells and Cl– fluxes across glioma cell membranes.2,3  Inhibition of Cl– channels with CTX prevents cell volume changes, and in turn, inhibits tumor cell invasion and migration.4-6 

Immunohistochemical studies show that CTX specifically and selectively binds to glioma cell lines and to tissue biopsies from patients with various malignant gliomas and other embryonic related tumors of neuroectodermal origin but not to normal brain.

These findings have lead to clinical evaluation for the therapeutic and diagnostic use of CTX a synthetic derivate in patients with malignant glioma.  This derivative of CTX has been shown to selectively label human gliomas in vivo and in vitro and demonstrated all of the known physical and biological activities of the naturally occurring CTX.7,8

Further studies on the interaction of CTX with Cl– channels suggest that these effects are indirect; affinity purification with a recombinant CTX identified the matrix metalloproteinase-2 (MMP2) as CTX binder in glioma cells. The actual receptor for CTX appears to be a protein complex that contains MMP2 and Cl– Channel-3 (ClC-3). Binding of CTX induces endocytosis of this complex and hence the ClC-3 channels. This finding might explain the irreversible action of CTX and its relatively slow time course of Cl– channel blockage.9,10
 
Alomone Labs is pleased to offer Chlorotoxin (#RTC-450), expressed in E. coli and purified to homogeneity.