Capricorn Scientific成立于2013年，是一家新的德国细胞培养基，血清和试剂生产商。我们生产的主要重点是生产用于生物技术，诊断和研究应用的高质量动物血清。严格的测试程序以及全面的可追溯性记录和文档保证了我们产品的和可重复的质量。
作为一家规模较小的公司，我们可以灵活地提供各种传统和定制产品，以更低的成本，更短的交付周期和个性化的客户服务满足当今市场不断增长的需求。
我们的技术，经验丰富的专家团队多年来一直致力于细胞培养领域。我们致力于为活跃的细胞培养公司提供高质量，可靠性和服务。
Capricorn Scientific Hy-A说明书
HybridomaPlus Protein-free Medium for Hybridoma Cells and Monoclonal Antibody Production with L-Glutamine
杂交瘤细胞无蛋白培养基及L-谷氨酰胺制备单克隆抗体的研究
货号：Hy-A(500毫升)
产品阐述，产品说明
杂交瘤加是一种无蛋白质的、可随时使用的培养基，不含多肽生长或附着因子，或可能使下游加工和终产品纯化复杂化的中介物。H型 此外，YbridomaPlus还能很好地用于制备单克隆抗体的血清培养基，也可作为一种生长介质。
商品概述
pH 7.0 – 7.5
内毒素≤1 EU/ml
细胞培养试验
不育试验
储存2°C至8°C
重要信息
-杂交瘤加需要补充胆固醇补充剂或其他来源的胆固醇，以生长依赖胆固醇的细胞系(例如NS0和衍生物)。
-杂交瘤加不含胰岛素或转铁蛋白。
-这种介质不含表面活性剂。如果用于搅拌悬浮培养，添加0.1%Pluronic F-68。
-在大多数情况下，抗生素是不必要的，也不是建议的。然而，在需要抗生素的地方，大多数普通抗生素都与杂交瘤加兼容，包括青霉素/链霉菌。 在，庆大霉素，抗PPLO，亚诺辛，两性霉素B。不要使用硫酸卡那霉素或新霉素硫酸盐。
培养条件
文化类型：暂停
培养容器：摇瓶、滚筒瓶或生物反应器。
温度范围：36℃至38℃
孵化器气氛：空气中5%至10%CO2的加湿空气。确保适当的气体交换，尽量减少文化暴露在光线下。
恢复，复原
在37°C水浴中快速解冻(<1分钟)冷冻细胞。
将冷冻瓶的全部内容转移到不含抗生素的30毫升预温杂交瘤加的组织培养瓶中。
37°C在空气中5%CO2的加湿环境中孵育。
4.解冻后3~5天传代细胞。
使杂交瘤细胞适应杂交瘤+
成功的适应将取决于特定的杂交瘤细胞株和所使用的培养条件。我们建议将原始培养基中的备份培养保持到成功为止。 新的媒介已经实现。
注：细胞存活率至少为90%，细胞在适应前处于对数中期生长阶段，这是至关重要的。
直接适应，直接适应作用
传代杂交瘤细胞在常规培养基中加入5%~10%血清或其他无血清培养基培养成预温杂交瘤细胞。在适应过程中，播种密度应加倍。 细胞株的正常播种密度。
监测细胞生长，直到活细胞密度达到1×106个活细胞/ml。将细胞传代至1~2×105个活细胞密度为1~2×105个/ml。
继续监测和传代细胞3至5代，直到达到一致的生长。
注：如果使用直接适应法在3~5通道上观察到次优性能，则使用顺序适应法。
顺序适应
传代杂交瘤细胞在常规培养基中加入5%~10%的血清或其他无血清培养基培养成25：75的新鲜杂交瘤细胞，与原培养基的比例为25：75。适应程序期间 种子密度为正常播种密度的两倍。
监测细胞生长，直到活细胞密度达到1×106个活细胞/ml。继代培养细胞(稀释为1~2×105个活细胞/ml)，逐步增加新鲜杂交瘤细胞与Ori的比例。 继代培养基(50：50，75：25，90：10，100杂交加)。每个步骤可能需要多个通道。
继续监测和传代细胞，直到细胞持续生长。在*杂交中进行了几次连续生长和存活的传代后，这种培养被认为是无性的。 [医][=pulmonary thromboembolic disease]肺动脉血栓栓塞疾病.
冷冻保存，低温贮藏
在组织培养瓶中准备所需数量的细胞，在生长中期收获，活率>90%。保留条件培养基准备冷冻保存培养基。
测定活细胞密度，计算冷冻保存培养基所需体积，得出终细胞密度为0.5~1×107个/ml。
在预定使用日制备92.5%培养基(新鲜培养基与条件培养基的比例为50：50)7.5%DMSO的要求体积。过滤，消毒，4°C保存，直到使用 。重要的是：条件培养基应从高活力的中对数培养细胞中获得.
100×g离心收集细胞5~10 min。将球团置于预先确定的4°C冷冻保存介质中。
根据制造商的规格将此悬浮液配发到冷冻瓶中(即2毫升冰瓶中的1毫升)。
按照标准程序(每分钟减少1°C)，在自动化或手动控制的冷冻设备中实现低温保存。
将冷冻细胞转移到液氮(蒸汽相)，建议储存在-200°C至-125°C。
预防措施及免责声明
本产品仅供研究用。请查阅材料安全数据表，以获取有关危险和安全处理做法的信息。
Pluronic是巴斯夫的注册商标。
胎儿牛血清和其他血清
我们生产和测试了我们的生物加工和细胞培养血清产品，用于不同的哺乳动物细胞培养应用。我们提供各种各样的特色血清：
- 胎牛血清（FBS）
- 其他牛血清
- 其他动物血清
- 人类血清
细胞培养基
液体培养基为所有细胞培养应用提供基本营养。我们的每种高质量细胞培养基都是根据初公布的配方或对培养基的一致性能和稳定性*的修饰而制造的。使用摩羯座培养基是保证细胞成功生长的宜方法。
我们提供各种产品：
- 经典媒体
- 特别媒体
- 粉末介质
试剂和补品
来自摩羯座的试剂和补充剂允许您定制细胞培养系统的生长条件。所有产品都经过严格测试和设计，以提供可重复性和良性能。
我们提供各种各样的产品：
- 牛血清白蛋白
- 拾遗
- 分离
- 抗生素
- 细胞分离
- 冷冻保存
- 去除支原体
- 转染
- 细胞遗传学
- 缓冲盐解决方案
Capricorn Scientific产品目录：
|
货号 |
品名 |
规格 |
价格 |
|
DMEM-LPA |
DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate |
500 ml |
7.50 € |
|
DMEM-LPSTA |
DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate |
500 ml |
9.50 € |
|
DMEM-LPXA |
DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate |
500 ml |
7.50 € |
|
DMEM-HA |
DMEM High Glucose (4.5 g/l), with L-Glutamine |
500 ml |
6.40 € |
|
DMEM-HHSTA |
DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES |
500 ml |
9.50 € |
|
DMEM-HPA |
DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate |
500 ml |
7.50 € |
|
DMEM-HPSTA |
DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate |
500 ml |
7.00 € |
|
DMEM-HPXA |
DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate |
500 ml |
7.50 € |
|
DMEM-HXA |
DMEM High Glucose (4.5 g/l), w/o L-Glutamine |
500 ml |
6.40 € |
|
DMEM-HXRXA |
DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red |
500 ml |
10.50 € |
|
DMEM-12-A |
DMEM/Ham’s F-12, with L-Glutamine |
500 ml |
8.60 € |
|
DMEM-12-HXA |
DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES |
500 ml |
8.60 € |
|
HAM-12-A |
Ham’s F-12, with L-Glutamine |
500 ml |
9.50 € |
|
IMDM-A |
IMDM, with L-Glutamine, without Supplements |
500 ml |
9.00 € |
|
L15-XA |
Leibovitz’s L-15 Medium, w/o L-Glutamine |
500 ml |
12.40 € |
|
MCC-A |
McCoy’s Medium, with L-Glutamine |
500 ml |
12.70 € |
|
M199-A |
Medium 199, with L-Glutamine |
500 ml |
8.90 € |
|
MEM-A |
MEM with Earle’s Salts, with L-Glutamine |
500 ml |
7.20 € |
|
MEM-STA |
MEM with Earle’s Salts, with Stable Glutamine |
500 ml |
9.40 € |
|
MEM-XA |
MEM with Earle’s Salts, w/o L-Glutamine |
500 ml |
6.40 € |
|
MEMA-RXA |
MEM Alpha Modification, w/o L-Glutamine, with Nucleosides |
500 ml |
19.90 € |
|
MEMA-XRXA |
MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides |
500 ml |
13.00 € |
|
RPMI-A |
RPMI 1640, with L-Glutamine |
500 ml |
6.40 € |
|
RPMI-HA |
RPMI 1640, with L-Glutamine, with 25 mM HEPES |
500 ml |
9.90 € |
|
RPMI-HXA |
RPMI 1640, w/o L-Glutamine, with 25 mM HEPES |
500 ml |
9.50 € |
|
RPMI-STA |
RPMI 1640, with Stable Glutamine |
500 ml |
8.40 € |
|
RPMI-XA |
RPMI 1640, w/o L-Glutamine |
500 ml |
5.80 € |
|
RPMI-XRXA |
RPMI 1640, w/o L-Glutamine, w/o Phenol Red |
500 ml |
9.90 € |
|
FM1-F |
FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum |
50 ml |
65.00 € |
|
FM2-F |
FreezeMe Two, Cryopreservation Medium Serum-free |
50 ml |
70.00 € |
|
TP-A |
TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine |
500 ml |
29.90 € |
|
HP-A |
HybridomaPrime, Hybridoma Medium, Serum-free |
500 ml |
34.90 € |
|
HY-A |
HybridomaPlus, Hybridoma Medium, Protein-free |
500 ml |
39.90 € |
|
FP-A |
FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine |
500 ml |
29.90 € |
|
ERP-B |
EndoPrime Complete Medium for C*tion of Primary Cells |
100 ml |
on request |
|
ERP-A |
EndoPrime Complete Medium for C*tion of Primary Cells |
500 ml |
on request |
|
HPR-B |
HepaPrime Complete Medium for C*tion of Primary Hepatocytes |
100 ml |
on request |
|
HPR-A |
HepaPrime Complete Medium for C*tion of Primary Hepatocytes |
500 ml |
on request |
|
NKR-G |
NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells |
20 ml |
on request |
|
NKR-B |
NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells |
100 ml |
on request |
|
CHOA-500ML |
CHO-ONE A |
500 ml |
45.00 € |
|
CHOE-500ML |
CHO-ONE E |
500 ml |
49.00 € |
|
CHOE-1000ML |
CHO-ONE E |
1000ml |
89.00 € |
|
CHOF1-100ML |
CHO-ONE F1 |
100 ml |
23.00 € |
|
CHOF1-500ML |
CHO-ONE F1 |
500 ml |
95.00 € |
|
CHOF2-10ML |
CHO-ONE F2 |
10 ml |
15.00 € |
|
CHOF2-50ML |
CHO-ONE F2 |
50 ml |
49.00 € |
|
CHO-K1 |
CHO-ONE Starter Kit |
|
169.00 € |
|
CHO-K2 |
CHO-ONE Expansion Kit |
|
950.00 € |
|
DMEM-LPA-P10 |
DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate |
10 L |
14.90 € |
|
DMEM-LPA-P50 |
DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate |
50 L |
62.90 € |
|
DMEM-HA-P10 |
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
10 L |
15.50 € |
|
DMEM-HA-P50 |
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
50 L |
66.50 € |
|
DMEM-HPA-P10 |
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate |
10 L |
15.50 € |
|
DMEM-HPA-P50 |
DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate |
50 L |
66.50 € |
|
MEM-A-P10 |
MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
10 L |
14.90 € |
|
MEM-A-P50 |
MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
50 L |
62.90 € |
|
RPMI-A-P10 |
RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
10 L |
15.50 € |
|
RPMI-A-P50 |
RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate |
50 L |
66.50 € |
|
RPMI-HA-P10 |
RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate |
10 L |
20.10 € |
|
RPMI-HA-P50 |
RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate |
50 L |
89.90 € |
|
FBS-52B |
Fetal Bovine Serum, Collected in Australia |
100 ml |
on request |
|
FBS-52A |
Fetal Bovine Serum, Collected in Australia |
500 ml |
on request |
|
FBS-12B |
Fetal Bovine Serum, Collected in South America |
100 ml |
on request |
|
FBS-12A |
Fetal Bovine Serum, Collected in South America |
500 ml |
on request |
|
FBS-22B |
Fetal Bovine Serum, Collected in USA |
100 ml |
on request |
|
FBS-22A |
Fetal Bovine Serum, Collected in USA |
500 ml |
on request |
|
FBS-HI-12B |
Fetal Bovine Serum, Heat Inactivated, Collected in South America |
100 ml |
on request |
|
FBS-HI-12A |
Fetal Bovine Serum, Heat Inactivated, Collected in South America |
500 ml |
on request |
|
FBS-HI-22B |
Fetal Bovine Serum, Heat Inactivated, Collected in USA |
100 ml |
on request |
|
FBS-HI-22A |
Fetal Bovine Serum, Heat Inactivated, Collected in USA |
500 ml |
on request |
|
FBS-11B |
Fetal Bovine Serum Advanced, Collected in South America |
100 ml |
on request |
|
FBS-11A |
Fetal Bovine Serum Advanced, Collected in South America |
500 ml |
on request |
|
FBS-HI-11B |
Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America |
100 ml |
on request |
|
FBS-HI-11A |
Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America |
500 ml |
on request |
|
FBS-TET-12B |
Fetal Bovine Serum, Tetracycline Negative, Collected in South America |
100 ml |
on request |
|
FBS-TET-12A |
Fetal Bovine Serum, Tetracycline Negative, Collected in South America |
500 ml |
on request |
|
FBS-ES-22B |
Fetal Bovine Serum, ES cell pre-tested, Collected in USA |
100 ml |
on request |
|
FBS-ES-22A |
Fetal Bovine Serum, ES cell pre-tested, Collected in USA |
500 ml |
on request |
|
FBS-DIA-12B |
Fetal Bovine Serum, Dialysed, Collected in South America |
100 ml |
on request |
|
FBS-DIA-12A |
Fetal Bovine Serum, Dialysed, Collected in South America |
500 ml |
on request |
|
FBS-IG-12B |
Fetal Bovine Serum, IgG Stripped, Collected in South America |
100 ml |
on request |
|
FBS-IG-12B |
Fetal Bovine Serum, IgG Stripped, Collected in South America |
500 ml |
on request |
|
FBS-ED-12F |
Fetal Bovine Serum Exosome Depleted |
50 ml |
on request |
|
FBS-ED-12B |
Fetal Bovine Serum Exosome Depleted |
100 ml |
on request |
|
FBS-ED-12A |
Fetal Bovine Serum Exosome Depleted |
500 ml |
on request |
|
NCS-1B |
Newborn Calf Serum |
100 ml |
12.00 € |
|
NCS-1A |
Newborn Calf Serum |
500 ml |
38.00 € |
|
NCS-HI-1B |
Newborn Calf Serum, Heat Inactivated |
100 ml |
13.10 € |
|
NCS-HI-1A |
Newborn Calf Serum, Heat Inactivated |
500 ml |
43.00 € |
|
CS-1B |
Calf Serum |
100 ml |
8.00 € |
|
CS-1A |
Calf Serum |
500 ml |
29.50 € |
|
ABS-1B |
Adult Bovine Serum |
100 ml |
8.00 € |
|
ABS-1A |
Adult Bovine Serum |
500 ml |
29.50 € |
|
ABS-1C |
Adult Bovine Serum |
1000 ml |
on reques |
|
HOS-1B |
Horse Serum |
100 ml |
7.20 € |
|
HOS-1A |
Horse Serum |
500 ml |
32.00 € |
|
DHS-1B |
Donor Horse Serum |
100 ml |
9.00 € |
|
DHS-1A |
Donor Horse Serum |
500 ml |
35.00 € |
|
GOA-1B |
Goat Serum |
100 ml |
24.00 € |
|
GOA-1A |
Goat Serum |
500 ml |
105.00 € |
|
POR-1B |
Porcine Serum |
100 ml |
10.50 € |
|
POR-1A |
Porcine Serum |
500 ml |
39.00 € |
|
MOU-1B |
Mouse Serum |
100 ml |
499.00 € |
|
RAT-1B |
Rat Serum |
100 ml |
499.00 € |
|
RAB-1B |
Rabbit Serum |
100 ml |
99.00 € |
|
HUM-3B |
Human Serum, Type AB, Male |
100 ml |
134.00 € |
|
BSA-1U |
Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA |
50 g |
56.00 € |
|
BSA-1S |
Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA |
100 g |
77.00 € |
|
BSA-1V |
Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA |
250 g |
on request |
|
BSA-1T |
Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA |
500 g |
299.00 € |
|
BSA-FAF-1U |
Bovine Serum Albumin, Fatty Acid Free, Origin USA |
50 g |
99.00 € |
|
BSA-FAF-1S |
Bovine Serum Albumin, Fatty Acid Free, Origin USA |
100 g |
178.00 € |
|
BSA-PF-1U |
Bovine Serum Albumin, Protease Free, Origin USA |
50 g |
88.00 € |
|
BSA-PF-1S |
Bovine Serum Albumin, Protease Free, Origin USA |
100 g |
151.00 € |
|
HSA-1R |
Human Serum Albumin |
10 g |
56.00 € |
|
HSA-1U |
Human Serum Albumin |
50 g |
227.00 € |
|
HSA-1S |
Human Serum Albumin |
100 g |
420.00 € |
|
N2-K |
N2 Supplement (100x), Serum-free |
5 ml |
45.00 € |
|
C21-H |
NCS21 Supplement (50x), Serum-free |
10 ml |
45.00 € |
|
ITS-H |
ITS (100x), Insulin, Transferrin, Selenium |
10 ml |
25.00 € |
|
HCS-F |
Hybridoma Supplément, Serum-free |
50 ml |
99.00 € |
|
GLN-B |
L-Glutamine, 200 mM |
100 ml |
8.00 € |
|
STA-B |
Stable Glutamine, 200 mM |
100 ml |
14.50 € |
|
NPY-B |
Sodium Pyruvate, 100 mM |
100 ml |
10.50 € |
|
NEAA-B |
MEM Nonessential Amino Acids (100x) |
100 ml |
8.50 € |
|
ACC-1B |
Accutase Cell Detachment Solution |
100 ml |
26.00 € |
|
ACM-1G |
AccuMax Cell Detachment Solution |
20 ml |
29.00 € |
|
ACM-1F |
AccuMax Cell Detachment Solution |
50 ml |
69.00 € |
|
TRY-1B |
Trypsin-EDTA (0.05 %) in DPBS (1x) |
100 ml |
7.00 € |
|
TRY-1B10 |
Trypsin-EDTA (0.5 %) in DPBS (10x) |
100 ml |
11.50 € |
|
TRY-2B |
Trypsin (0.25 %) in DPBS (1x) |
100 ml |
7.00 € |
|
TRY-2B10 |
Trypsin (2.5 %) in DPBS (10x) |
100 ml |
11.50 € |
|
TRY-3B |
Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red |
100 ml |
9.50 € |
|
TRY-4B |
Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red |
100 ml |
8.50 € |
|
AMP-B |
Amphotericin B Solution (250 µg/ml) |
100 ml |
14.00 € |
|
AAS-B |
Antibiotic/Antimycotic Solution (100x) |
100 ml |
14.00 € |
|
GEN-10B |
Gentamicin Solution (10 mg/ml) |
100 ml |
50.00 € |
|
PS-B |
Penicillin/Streptomycin (100x) |
100 ml |
8.00 € |
|
PSG-B |
Penicillin/Streptomycin (100x), with L-Glutamine |
100 ml |
15.00 € |
|
MYX-B |
MycoXpert, Mycoplasma Removal Reagent (50x) |
100 ml |
99.00 € |
|
LSM-B |
Lymphocytes Separation Media, Density 1.077 g/ml |
100 ml |
12.00 € |
|
LSM-A |
Lymphocytes Separation Media, Density 1.077 g/ml |
500 ml |
36.50 € |
|
FM1-F |
FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum |
50 ml |
65.00 € |
|
FM2-F |
FreezeMe Two, Cryopreservation Medium Serum-free |
50 ml |
70.00 € |
|
MYX-B |
MycoXpert, Mycoplasma Removal Reagent (50x) |
100 ml |
99.00 € |
|
PBS-10XA |
Dulbecco’s PBS (10x), w/o Ca & Mg, w/o Phenol Red |
500 ml |
13.00 € |
|
PBS-1A |
Dulbecco’s PBS (1x), w/o Ca & Mg, w/o Phenol Red |
500 ml |
6.50 € |
|
PBS-2A |
Dulbecco’s PBS (1x), with Ca & Mg, w/o Phenol Red |
500 ml |
8.00 € |
|
HBSS-1A |
Hanks’ Balanced Salts (1x), with Ca & Mg, w/o Phenol Red |
500 ml |
7.50 € |
|
HBSS-2A |
Hanks’ Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red |
500 ml |
7.00 € |
|
HBSS-3A |
Hanks’ Balanced Salts (1x), w/o Ca & Mg, with Phenol Red |
500 ml |
7.50 € |
|
HEP-B |
HEPES Buffer Solution (1 M) |
100 ml |
39.00 € |
|
PBS-1A-P10 |
Dulbecco’s PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red |
10 L |
10.90 € |
|
PBS-1A-P50 |
Dulbecco’s PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red |
50 L |
43.10 € |
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