标签归档：pngase

Ludger调整内糖苷酶和外糖苷酶价格，即日生效！

发表于由

Ludger调整内糖苷酶和外糖苷酶价格，即日生效！

 

Ludger2019年9月新版价格表如下：

 

huohao pingming guige jiage
LZ-rPNGaseF-KIT Recombinant PNGase F enzyme 150 μL 12548 
E-PNG01 PNGase F (Peptide N Glycosidase F) 0.3 U / 60 µL 10081 
E-PNG01-200
*formerly E-PNG05		 PNGase F (Peptide N Glycosidase F) 1 U / 200 µL 27563 
E-rPNG01 Recombinant PNGase F 0.3 U / 60 µL 10081 
E-EF01 Endoglycosidase F1 1 U / 60 µL 10081 
E-EF02 Endoglycosidase F2 0.3 U / 60 µL 10081 
E-EF03 Endoglycosidase F3 0.33 U / 60 µL 10081 
E-EH02 Endoglycosidase H 0.3 U / 60 µL 10081 
E-G001 O-glycosidase 75 mU / 60 µL 10081 
KE-DG01 Enzymatic CarboRelease Kit 20 µL each enzyme 14327 
KE-DGMX Enzymatic DeGlycoMx Kit 20 µL premix 7970 
E-XBG01 Endo-Beta-Galactosidase 0.9 U / 60 µL 10081 
LZ-CER-HM-KIT Ceramide glycanase kit 55 µL 5598 
E-S001 Sialidase Au Alpha-(2-3,6,8,9) 0.3 U / 60 µL 10081 
E-S005 Sialidase Cp Alpha-(2-3,6) 0.9 U / 60 μL 10081 
E-S007 Sialidase Sp Alpha-(2-3) 0.3 U / 60 µL 10081 
LZ-ACASE-KIT Sialate O-acetylesterase kit 50 µL 10959 
E-BG07 Beta-(1-4)-galactosidase 0.18 U / 60 µL 10081 
E-BG02 Beta-(1-3,4,6)-galactosidase 0.5 U / 200 µL 10081 
E-AG02  *formerly E-AG01 Alpha-(1-3,6)-galactosidase 0.4 U / 60 µL 10081 
E-GL01 Beta-N-acetylglucosaminidase 2.4 U / 60 µL 10081 
E-AM01 Alpha-(1-2,3,6)-mannosidase 0.6 U / 60 µL 10081 
E-AM02 Alpha-(1,6) core mannosidase 0.6 U / 60 µL 10081 
E-F134 Alpha-(1-3,4)-fucosidase 30 mU / 60 µL 10081 
LL-HYDRAZ-A2† Ludger Liberate Hydrazinolysis Kit 12 17980 
LL-ORELA-A2 Ludger Liberate Orela Kit 12 10698 
LL-MR-A2† Ludger Liberate Membrane Release kit 96 10200 
LT-KAB-A2 2-AB Glycan Labeling Kit up to 30 6120 
LT-KAA-A2 2-AA Glycan Labeling Kit up to 30 6120 
LT-KAB-VP24 2-AB Glycan Labeling Kit, 2PB reductant 24 6120 
LT-KAA-VP24 2-AA Glycan Labeling Kit, 2PB reductant 24 6120 
LT-KAB-VP96 2-AB Glycan Labeling Kit, 2PB reductant 96 14279 
LT-KPROC-VP24 Procainamide Glycan Labeling Kit, 2PB reductant 24 8278 
LT-KPROC-24 Procainamide Glycan Labeling Kit, sodium cyanoborohydride reductant 24 8278 
LT-KPROC-96 Procainamide Glycan Labeling Kit, sodium cyanoborohydride reductant 96 23198 
LT-VTAG-C30 Glycan Release and Labeling Kit 30 25618 
LT-VTAG-24 Glycopeptide Labeling and Enrichment Kit 24 14279 
LT-PERMET-96 Permethylation Kit 96 13165 
LT-PERMET-VP96 Permethylation Kit (without methyl iodide) 96 11172 
LT-KDMB-A1 DMB Sialic Acid Release & Labeling Kit 22 7662 
LT-MONO-96 Monosaccharide Release & Labeling Kit 96 14066 
LC-A-24 LudgerClean A cartridges 24 cartridges 10413 
LC-CEX-A6 LudgerClean CEX cartridges 6 cartridges 3060 
LC-EB10-A6 LudgerClean EB10 Cartridges 6 cartridges 3345 
LC-EC50-24 LudgerClean EC50 Cartridges 24 cartridges 8539 
LC-S-A6 LudgerClean S Cartridges 6 cartridges 2799 
LC-T1-A6 LudgerClean T1 Cartridges* 6 cartridges 2799 
LC-PBM-96 LudgerClean Protein Binding Membrane Plate 1 x 96 well plate 2870 
LC-PROC-96 LudgerClean Plate for Procainamide Glycan Clean Up 1 x 96 well plate 2609 
LC-PERMET-96 LudgerClean Plate for pre-Permethylation Clean Up 1 x 96 well plate 2609 
LC-VAC-MANIFOLD-KIT Vacuum manifold in 96 plate format kit 26946 
LC-VAC-TRAP-KIT Vacuum trap kit kit 9986 
LP-COLLPLATE-2ML-96 Collection Plate Pack 5 per pack 3938 
LP-COLLPLATE-LID-96 Collection Plate Lid Pack 5 per pack 4270 
LP-HOLDER-96 Cartridge Holder ea 6215 
LP-PLUG-96 Plug pack 12 strips of 8 plugs 1447 
LS-N1-4.6×10 LudgerSep N1 amide Guard Column 4.6 x 10 mm, 5 m 14066 
LS-N1-4.6×250 LudgerSep N1 amide HPLC Column 4.6 x 250 mm, 5 m 38403 
LS-N2-4.6×150 LudgerSep N2 amide HPLC Column 4.6 x 150mm, 3 m 36837 
LS-N2-2.0×150 LudgerSep N2 amide HPLC Column 2.0 x 150 mm, 3 m 36837 
LS-N-BUFFX40 LudgerSep N Buffer x40 Concentrate 50ml, makes 2 litres buffer 2562 
LS-C2-4.6×50 LudgerSep C2 anion exchange HPLC Column 4.6 x 50 mm 24005 
LS-C2-4.6×150 LudgerSep C2 anion exchange HPLC Column 4.6 x 150 mm 33398 
LS-C3-7.5×75 LudgerSep C3 anion exchange HPLC Column 7.5 x 75 mm 52730 
LS-C-BUFFX4 LudgerSep C Buffer x4 Concentrate 50ml, makes 250 ml buffer 1992 
LS-R1-4.6×150 LudgerSep R1 HPLC Column 4.6 x 150 mm 17577 
LS-R2-4.6×150 LudgerSep R2 HPLC Column 4.6 x 150 mm 17577 
LS-UR2-2.1×50 LudgerSep uR2 UHPLC Column – monosaccharide 2.1 x 50 mm 17577 
LS-UR2-2.1×100 LudgerSep uR2 UHPLC Column – sialic acid 2.1 x 100 mm 17577 
LS-R-BPTX10 LudgerSep R BPT solvent x10 Concentrate 50ml, makes 500ml solvent 2301 
CN-NA4-20U NA4 Glycan (A4G4) 20 g 6499 
CN-NA4-10U NA4 Glycan (A4G4) 10 g 4578 
CN-NGA4-20U NGA4 Glycan (A4) 20 g 6736 
CN-NGA4-10U NGA4 Glycan (A4) 10 g 4720 
CN-A3-20U A3 Glycan (A3G3S3) 20 g 4768 
CN-A3-10U A3 Glycan (A3G3S3) 10 g 3321 
CN-NA3-20U NA3 Glycan (A3G3) 20 g 5029 
CN-NA3-10U NA3 Glycan (A3G3) 10 g 3511 
CN-NGA3-20U NGA3 Glycan (A3) 20 g 5550 
CN-NGA3-10U NGA3 Glycan (A3) 10 g 3914 
CN-A2F-20U A2F Glycan (FA2G2S2, G2FS2) 20 g 7187 
CN-A2F-10U A2F Glycan (FA2G2S2, G2FS2) 10 g 5076 
CN-A1F-20U A1F Glycan (FA2G2S1, G2FS1) 20 g 7187 
CN-A1F-10U A1F Glycan (FA2G2S1, G2FS1) 10 g 5076 
CN-NA2F-20U NA2F Glycan (FA2G2, G2F) 20 g 7472 
CN-NA2F-10U NA2F Glycan (FA2G2, G2F) 10 g 5242 
CN-NGA2F-20U NGA2F Glycan (FA2, G0F) 20 g 8753 
CN-NGA2F-10U NGA2F Glycan (FA2, G0F) 10 g 6143 
CN-FA2G1-20U FA2G1 Glycan (G1F) 20 g 7472 
CN-FA2G1-10U FA2G1 Glycan (G1F) 10 g 5242 
CN-A2-20U A2 Glycan (A2G2S2, G2S2) 20 g 7187 
CN-A2-10U A2 Glycan (A2G2S2, G2S2) 10 g 5076 
CN-A1-20U A1 Glycan (A2G2S1, G2S1) 20 g 7187 
CN-A1-10U A1 Glycan (A2G2S1, G2S1) 10 g 5076 
CN-NA2-20U NA2 Glycan (A2G2, G2) 20 g 4436 
CN-NA2-10U NA2 Glycan (A2G2, G2) 10 g 3084 
CN-A2G1-20U A2G1 Glycan (G1) 20 g 7472 
CN-NGA2-20U NGA2 Glycan (A2, G0) 20 g 6665 
CN-NGA2-10U NGA2 Glycan (A2, G0) 10 g 4673 
CN-MAN9-20U Man-9 Glycan 20 g 7472 
CN-MAN9-10U Man-9 Glycan 10 g 5242 
CN-MAN8-20U Man-8 Glycan 20 g 6736 
CN-MAN8-10U Man-8 Glycan 10 g 4720 
CN-MAN7-20U Man-7 Glycan 20 g 6736 
CN-MAN7-10U Man-7 Glycan 10 g 4720 
CN-MAN6-20U Man-6 Glycan 20 g 4863 
CN-MAN6-10U Man-6 Glycan 10 g 3439 
CN-MAN5-20U Man-5 Glycan 20 g 4863 
CN-MAN5-10U Man-5 Glycan 10 g 3439 
CN-M3N2-20U M3N2 (Man-3) Glycan 20 g 5930 
CN-M3N2-10U M3N2 (Man-3) Glycan 10 g 4151 
CLIBN-IGG-01 IgG N glycan library 25 µg 4436 
CLIBN-FETUIN-01 Fetuin N glycan library Approx. 7.5 µg 4436 
SA-MAB4 MAb 4 Glycan Reference Panel 10 µg 10484 
CN-HYBRID-20U Hybrid Glycan – discontinued 20 g 停产
CN-HYBRID-10U Hybrid Glycan – discontinued 10 g 停产
SA-CORE1 Galactose-ß-(1-3)-GalNAc Glycan, O-linked 20 µg 8444 
CLIBO-FETUIN-01 Fetuin O glycan library Released from 30 µg fetuin 4436 
CPM-IGG-01 N-glycan IgG library, permethylated 20 MS runs 2372 
CPM-C13-IGG-01 N-glycan IgG library, permethylated with heavy (13C) methyl iodide 20 MS runs 2372 
CAB-NA4-01 NA4 Glycan (A4G4), 2-AB Labeled 100 pmol 2230 
CAB-NGA4-01 NGA4 Glycan (A4), 2-AB Labeled 100 pmol 2230 
CAB-A3-01 A3 Glycan (A3G3S3), 2-AB Labeled 100 pmol 2230 
CAB-NA3-01 NA3 Glycan (A3G3), 2-AB Labeled 100 pmol 2230 
CAB-NGA3-01 NGA3 Glycan (A3), 2-AB Labeled 100 pmol 2230 
CAB-A2F-01 A2F Glycan (FA2G2S2, G2FS2), 2-AB Labeled 100 pmol 2230 
CAB-A1F-01 A1F Glycan (FA2G2S1, G2FS1), 2-AB Labeled 100 pmol 2230 
CAB-NA2F-01 NA2F Glycan (FA2G2, G2F), 2-AB Labeled 100 pmol 2230 
CAB-NGA2F-01 NGA2F Glycan (FA2, G0F), 2-AB Labeled 100 pmol 2230 
CAB-FA2G1-01 FA2G1 Glycan (G1F), 2-AB Labeled 100 pmol 2657 
CAB-FA2B-01 FA2B Glycan (G0B), 2-AB Labeled 100 pmol 3250 
CAB-FA2BG1-01 FA2BG1 Glycan (G1B), 2-AB Labeled 100 pmol 3250 
CAB-A2-01 A2 Glycan (A2G2S2, G2S2), 2-AB Labeled 100 pmol 2230 
CAB-A1-01 A1 Glycan (A2G2S1, G2S1), 2-AB Labeled 100 pmol 2230 
CAB-NA2-01 NA2 Glycan (A2G2, G2), 2-AB Labeled 100 pmol 2230 
CAB-A2G1-01 A2G1 Glycan (G1), 2-AB Labeled 100 pmol 3250 
CAB-NGA2-01 NGA2 Glycan (A2, G0), 2-AB Labeled 100 pmol 2230 
CAB-MAN9-01 Man-9 Glycan, 2-AB Labeled 100 pmol 2230 
CAB-MAN8-01 Man-8 Glycan, 2-AB Labeled 100 pmol 2230 
CAB-MAN7-01 Man-7 Glycan, 2-AB Labeled 100 pmol 2230 
CAB-MAN6-01 Man-6 Glycan, 2-AB Labeled 100 pmol 2230 
CAB-MAN5-01 Man-5 Glycan, 2-AB Labeled 100 pmol 2230 
CAB-M3N2-01 M3N2 (Man-3) Glycan, 2-AB Labeled 100 pmol 2230 
CAB-IGG-01 IgG N glycan library, 2-AB Labeled 200 pmol 5598 
CAB-GHP-30 2-AB Labeled Glucose Homopolymer Ladder 30 runs 2633 
CAB-ALPHA-GAL-01 Gal alpha 1-3 Gal beta 1-4 GlcNAc, 2-AB Labeled 100 pmol 3250 
CAB-HYBRID-01 Hybrid Glycan (Man5BA1), 2-AB Labeled – discontinued 100 pmol 停产
CAB-C1-01 2AB labeled core 1 O- glycan, C1 100 pmol 8492 
CAB-C1S(3)1-01 2AB labeled sialylated core 1 O glycan, C1S(3)1 100 pmol 8492 
CAB-C1S(3)1-02 2AB labeled sialylated core 1 O glycan, C1S(3)1 50 pmol 5693 
CAB-C2S(3,3)2-01 2AB labeled di-sialylated core 2 O glycan, C2S(3,3)2 100 pmol 8492 
CAB-C2S(3,3)2-02 2AB labeled di-sialylated core 2 O glycan, C2S(3,3)2 50 pmol 停产，特殊要求时可询价
CAB-C1S(3,6)2-01 2AB labeled di-sialylated core 1 O glycan, C1S(3,6)2 100 pmol 8492 
CAA-NA4-01 NA4 Glycan (A4G4), 2-AA Labeled 100 pmol 2230 
CAA-NGA4-01 NGA4 Glycan (A4), 2-AA Labeled 100 pmol 2230 
CAA-A3-01 A3 Glycan (A3G3S3), 2-AA Labeled 100 pmol 2230 
CAA-NA3-01 NA3 Glycan (A3G3), 2-AA Labeled 100 pmol 2230 
CAA-NGA3-01 NGA3 Glycan (A3), 2-AA Labeled 100 pmol 2230 
CAA-A2F-01 A2F Glycan (FA2G2S2, G2FS2), 2-AA Labeled 100 pmol 2230 
CAA-A1F-01 A1F Glycan (FA2G2S1, G2FS1), 2-AA Labeled 100 pmol 2230 
CAA-NA2F-01 NA2F Glycan (FA2G2, G2F), 2-AA Labeled 100 pmol 2230 
CAA-FA2G1-01 FA2G1 Glycan (G1F), 2-AA Labeled 100 pmol 2657 
CAA-NGA2F-01 NGA2F Glycan (FA2, G0F), 2-AA Labeled 100 pmol 2230 
CAA-FA2B-01 FA2B Glycan (G0B), 2-AA Labeled 100 pmol 3250 
CAA-FA2BG1-01 FA2BG1 Glycan (G1B), 2-AA Labeled 100 pmol 3250 
CAA-A2-01 A2 Glycan (A2G2S2, G2S2), 2-AA Labeled 100 pmol 2230 
CAA-A1-01 A1 Glycan (A2G2S1, G2S1), 2-AA Labeled 100 pmol 2230 
CAA-NA2-01 NA2 Glycan (A2G2, G2), 2-AA Labeled 100 pmol 2230 
CAA-A2G1-01 A2G1 Glycan (G1), 2AA Labeled 100 pmol 3250 
CAA-NGA2-01 NGA2 Glycan (A2, G0), 2-AA Labeled 100 pmol 2230 
CAA-MAN9-01 Man-9 Glycan, 2-AA Labeled 100 pmol 2230 
CAA-MAN8-01 Man-8 Glycan, 2-AA Labeled 100 pmol 2230 
CAA-MAN7-01 Man-7 Glycan, 2-AA Labeled 100 pmol 2230 
CAA-MAN6-01 Man-6 Glycan, 2-AA Labeled 100 pmol 2230 
CAA-MAN5-01 Man-5 Glycan, 2-AA Labeled 100 pmol 2230 
CAA-M3N2-01 M3N2 (Man-3) Glycan, 2-AA Labeled 100 pmol 2230 
CAA-GHP-30 2-AA Labeled Glucose Homopolymer Ladder 30 runs 2633 
CAA-ALPHAGAL-01 Gal alpha 1-3 Gal beta 1-4 GlcNAc, 2-AA Labeled 100 pmol 3250 
CAA-HYBRID-01 Hybrid Glycan (Man5BA1), 2-AA Labeled – discontinued 100 pmol 停产
CAPTS-A2F-01 A2F Glycan (FA2G2S2, G2FS2), APTS Labeled 20 pmol 1044 
CAPTS-A1F-01 A1F Glycan (FA2G2S1, G2FS1), APTS Labeled 20 pmol 1044 
CAPTS-NA2F-01 NA2F Glycan (FA2G2, G2F), APTS Labeled 20 pmol 1044 
CAPTS-FA2G1-01 FA2G1 Glycan (G1F), APTS Labeled 20 pmol 1044 
CAPTS-NGA2F-01 NGA2F Glycan (FA2, G0F), APTS Labeled 20 pmol 1044 
CAPTS-A2-01 A2 Glycan (A2G2S2, G2S2), APTS Labeled 20 pmol 1044 
CAPTS-A1-01 A1 Glycan (A2G2S1, G2S1), APTS Labeled 20 pmol 1044 
CAPTS-NA2-01 NA2 Glycan (A2G2, G2), APTS Labeled 20 pmol 1044 
CAPTS-NGA2-01 NGA2 Glycan (A2, G0), APTS Labeled 20 pmol 1044 
CAPTS-MAN5-01 Man-5 Glycan, APTS Labeled 20 pmol 1044 
CAPTS-IGG-01 IgG N-glycan library, APTS labeled 10 pmol 1044 
CPROC-A3-01 A3 Glycan (A3G3S3), Procainamide Labeled 20 pmol 2135 
CPROC-NA3-01 NA3 Glycan (A3G3), Procainamide Labeled 20 pmol 2135 
CPROC-A1F-01 A1F Glycan (FA2G2S1, G2FS1), Procainamide Labeled 20 pmol 2135 
CPROC-FA2G1-01 FA2G1 Glycan (G1F), Procainamide Labeled 20 pmol 2135 
CPROC-NA2-01 NA2 Glycan (A2G2S1, G2S1), Procainamide Labeled 20 pmol 2135 
CPROC-NGA2-01 NGA2 Glycan (A2), Procainamide Labeled 20 pmol 2135 
CPROC-MAN5-01 Man-5 Glycan, Procainamide Labeled 20 pmol 2135 
CPROC-MAN6-01 Man-6 Glycan, Procainamide Labeled 20 pmol 2135 
CPROC-MAN7-01 Man-7 Glycan, Procainamide Labeled 20 pmol 2135 
CPROC-MAN8-01 Man-8 Glycan, Procainamide Labeled 20 pmol 2135 
CPROC-MAN9-01 Man-9 Glycan, Procainamide Labeled 20 pmol 2135 
CPROC-IGG-02 IgG N-glycan library, Procainamide Labeled 50 pmol 3060 
CPROC-GHP-30 Glucose Homopolymer Ladder, Procainamide Labeled ea 2514 
CLIBN-FETUIN-01 Fetuin N glycan library Approx. 7.5 µg 4436 
CLIBO-FETUIN-01 Fetuin O glycan library Released from 30 µg fetuin 4436 
CLIBN-IGG-01 IgG N glycan library 25 µg 4436 
CAB-IGG-01 IgG N glycan library, 2-AB Labeled 200 pmol 5598 
CPROC-IGG-02 IgG N-glycan library, Procainamide Labeled 50 pmol 3060 
CAPTS-IGG-01 IgG N-glycan library, APTS Labeled 10 pmol 1044 
CPM-IGG-01 N-glycan IgG library, permethylated 20 MS runs 2372 
CPM-C13-IGG-01 N-glycan IgG library, permethylated with heavy (13C)  methyl iodide 20 MS runs 2372 
SA-MAB4 MAb 4 Glycan Reference Panel 10 µg 10484 
CM-NEU-AC-01 N-acetylneuraminic acid quantitative standard 1nmol 901 
CM-NEUAC-100 N-acetylneuraminic acid quantitative standard 100nmol 2538 
CM-NEU-GC-01 N-glycolyneuraminic acid quantitative standard 1nmol 901 
CM-NEUGC-100 N-glycolyneuraminic acid quantitative standard 100nmol 2538 
CM-NEU5,9AC2-01 N-acetylneuraminic acid qualitative standard >800pmol 1186 
CM-MONOMIX-10 Mix of six monosaccharide quantitative standards(glucosamine, galactosamine, galactose, mannose,glucose(dextrose) and fucose) 10nmols of each 8848 
CM-MONOMIX-10X3 Mix of six monosaccharide quantitative standards(glucosamine, galactosamine, galactose, mannose,glucose(dextrose) and fucose) 10nmols of each, pack of 3 17695 
CM-SRP-01 Sialic acid reference panel approx 1.25 nmol 712 
CM-XYLOSE-100 Xylose quantitative standard 100nmols 1898 
CM-MAN6P-10 Mannose 6 phosphate standard 10nmols 2111 
GCP-FET-05 Fetuin glycoprotein standard 500 µg 901 
GCP-FET-250U Fetuin glycoprotein standard 250 µg 593 
GCP-FET-50U-X4 Fetuin glycoprotein standard 4 x 50 µg 1660 
GCP-IGG-50U IgG glycoprotein standard 50 µg 593 
GCP-IGG-100U IgG glycoprotein standard 100 µg 901 
BQ-GPEP-A2G2S2-10U BioQuant A2G2S2 glycopeptide standard 3.49 nmol 4578 
GPEP-FA2-01 GPEP FA2 glycopeptide standard approx 6 µg 3202 
BQ-CN-MAN8-10U BioQuant quantitative Man-8 standard 10 µg 5906 
BQ-GPEP-A2G2S2-10U BioQuant A2G2S2 glycopeptide standard 3.49 nmol 4578 
BQ-CHITOTRIOSE-01 BioQuant chitotriose standard 5 nmol 4578 
BQ-CAB-CHI-01 BioQuant chitotriose standard, 2-AB Labeled 100 pmol 4578 
BQ-CAA-CHI-01 BioQuant chitotriose standard, 2-AA Labeled 100 pmol 4578 
CM-NEU-AC-01 N-acetylneuraminic acid quantitative standard 1nmol 901 
CM-NEU-GC-01 N-glycolyneuraminic acid quantitative standard 1nmol 901 
CM-MONOMIX-10 Mix of six monosaccharide quantitative standards(glucosamine, galactosamine, galactose, mannose,glucose(dextrose) and fucose)glucose(dextrose) and fucose) 10nmols of each 8848 
CM-MONOMIX-10X3 Mix of six monosaccharide quantitative standards(glucosamine, galactosamine, galactose, mannose,glucose(dextrose) and fucose)glucose(dextrose) and fucose) 10nmols of each, pack of 3 17695 
CM-XYLOSE-100 Xylose quantitative standard 100nmols 1898 
CM-MAN6P-10 Mannose 6 phosphate standard 10nmols 2111 

Ludger是一家生物科学公司，专门从事糖生物学医学应用的分析技术。该技术起源于英国牛津大学。它用于FDA和EMEA批准的生物制药的质量控制，可用于支持IND提交。

neb P0704L说明书

发表于由

neb P0704L说明书

PNGase F.

目录 ＃

 

 P0704S

  • 浓度

     500,000单位/毫升

    尺寸

  •   P0704L  75,000个单位

    价格表

    $ 718.00

PNGase F是从糖蛋白中去除几乎所有N-连接的寡糖的有效的酶促方法。PNGase F是酰胺酶，其在高甘露糖，杂合和复合寡糖的内部GlcNAc和天冬酰胺残基之间切割。

  • 通过SDS-PAGE和完整的ESI-MS测定，纯度≥95％
  • 非重组，没有可检测的内切糖苷酶F1，F2或F3污染
  • 储存在50％甘油中; 宜活动和稳定性长达24个月
  • 可在天然或变性条件下使用
  • 优化糖蛋白的去糖基化; 使N-聚糖核心寡糖保持完整，适合进一步分析

 

 

 

 

 
 

肽 – N-糖苷酶F，也称为PNGase F，是一种酰胺酶，可裂解来自N-连接糖蛋白的高甘露糖，杂合寡糖和复合寡糖的内层GlcNAc和天冬酰胺残基（1）

详细的特异性： 

当内部的GlcNAc残基与α1-3岩藻糖残基连接时，PNGase F不能从糖蛋白切割N-连接的聚糖。这种修饰常见于植物和一些昆虫糖蛋白中。

产品来源

PNGase F从脑膜炎黄杆菌（Flavobacterium meningosepticum）（3）中纯化，不含蛋白酶和Endo F活性。

提供的试剂

本产品随附以下试剂：

  存储在（°C） 浓度
糖蛋白变性缓冲液 -20 10 X.
NP-40 -20 10％
GlycoBuffer 2 -20 10 X.

 

应用功能

  • 糖蛋白分析
  • 从糖蛋白中去除高甘露糖，杂合和复杂的  N-聚糖
  • 无污染物（Endo F，蛋白酶等）

 

单位定义

一个单位定义为在37℃下在10μl的总反应体积中在1小时内从10μg变性的RNase B中除去> 95％的碳水化合物所需的酶量。

单位定义测定：
将10μgRNaseB用1X糖蛋白变性缓冲液（0.5％SDS，40mM DTT）在100℃变性10分钟。加入NP-40和GlycoBuffer 2后，加入两倍稀释的PNGase F，将反应混合物在37℃下温育1小时。通过SDS-PAGE显现反应产物的分离。

1X糖蛋白变性缓冲液
0.5％SDS 
40 mM DTT 

1X NP-40
1％NP-40，MilliQ-H 2 O

反应条件

1X GlycoBuffer 2 
在37°C孵育

1X GlycoBuffer 2 
50 mM磷酸钠
（pH值@ 25°C）

 

贮存温度

-20℃下

储藏条件

在 25℃下，20mM Tris-HCl 
50mM NaCl 
5mM EDTA 
50％甘油
pH 7.5

热灭活

75°C，10分钟

分子量

表观：36000道尔顿

  1. 由于SDS抑制PNGase F活性，因此必须使NP-40存在于反应混合物中。为什么这种非离子型去污剂抵消SDS抑制作用目前尚不清楚。
  2. 为了使天然糖蛋白去糖基化，可能需要更长的孵育时间以及更多的酶。
  3. PNGase F不会切割  含有核心α1-3岩藻糖的N-连接聚糖。
  4. 典型反应条件：请参阅协议