Polyplus 103-05N 说明书

世界*实验材料供应商Polyplus Transfection正式上海金畔为其中国代理,Polyplus Transfection在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔一直秉承为中国科研客户带来的产品,的服务, Polyplus Transfection就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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PolyPlus-transfection(PT)是一家位于法国的生物技术公司,目前已经通过了ISO9001:2000质量体系认证。该公司拥有十几年的的转染试剂研发经验,已经开发了一系列的转染试剂,如:基因的体内和体外转染试剂、寡核苷酸转染试剂和siRNA转染试剂等,可以根据不同的需要获得稳定或瞬时的转染效果。
JetPEITM转染试剂
该试剂是阳离子化的大分子多聚乙烯亚胺(PEI)及其衍生物,其与外源基因结合形成颗粒状,与细胞表面的蛋白多糖结合,通过细胞”内吞作用”进入细胞。PEI-复合体具有质子海绵的特性,能吸收溶酶体的H+,在复合体中形成酸性环境使核酸酶失活,保护DNA免受核酸酶的降解。进入细胞后,JetPEI复合体囊泡肿胀破裂,将DNA释放到细胞质中。
特点:
l 转染效率高,重复性好
l 可转染许多种细胞系,对粘附细胞和悬浮细胞同样有效
l 对原代细胞也具有很好的转染结果
l 也可以转染寡核苷酸
l 培养基可含或不含血清
l 极低的细胞毒性
l 方法简便,省时省力

 

jetPEI®-Macrophage

For primary macrophages, glial & dendritic cells

JETPEI ® -巨噬细胞

对于原发性巨噬细胞,神经胶质和树突状细胞

  • 原发性巨噬细胞,神经胶质和树突状细胞的有效转染
  • 易于使用协议
  • 与血清和抗生素兼容
  • 产品规格

    试剂

    JETPEI ® -巨噬细胞

    递送的分子

    脱氧核糖核酸

    应用

    原发性巨噬细胞,神经胶质和树突状细胞的DNA转染

    细胞类型

    原发性巨噬细胞,神经胶质和树突状细胞

    转数

    0.5毫升JETPEI的® -巨噬细胞转染试剂是足够在24孔板或160转染在60-mm培养皿中进行高达500转染

    存储

    4℃,长达12个月

    与提供

    150mM NaCl的

概要

JETPEI ®细胞-巨噬transfects 原发性巨噬细胞,神经胶质和树突细胞被认为难以转染。它包含结合到表达特定甘露糖的膜受体,如巨噬细胞的细胞的甘露糖缀合的线性聚乙烯亚胺。

JETPEI ® -巨噬细胞也是的细胞系如RAW 264.7转染试剂。

JETPEI ® -巨噬细胞是甘露糖轴承的线性聚乙烯亚胺设计用于增强表达在其细胞膜受体甘露糖细胞的转染。细胞定位是的甘露糖残基的特异性细胞表面受体的结合,导致DNA复合物的内化的结果。

 

目录编号 试剂的量 氯化钠的量
103-05N 0.5毫升 50毫升

0.5毫升jetPEI®细胞巨噬细胞转染试剂是足以在24孔板进行高达500转染。

 

Open Biosystems 产品报价信息

 ATR1740Arrest-In transfection reagent 0.5ml0.5 ML1773Open Biosystems

ATR1741Arrest-In transfection reagent 1ml (2×0.5ml)2 x .05 mL2101Open Biosystems
ATR1742Arrest-In transfection reagent 5ml (5x1ml)5 x 1 mL8667Open Biosystems
ATR1743Arrest-In transfection reagent 10ml (10x1ml)10 x 1 mL12082Open Biosystems
ATR4164Fluorescent Arrest-In Transfection Reagent (0.5ml)0.5 ML2101Open Biosystems
ATR4165Fluorescent Arrest-In transfection reagent (1.0mL)1 ML3152Open Biosystems
ATR4166Fluorescent Arrest-In Transfection Reagent (5x1ml)5 x 1 mL10046Open Biosystems
ATR4167Fluorescent Arrest-In Transfection Reagent  (10x1ml)10 x 1 mL14511Open Biosystems
BCA5101Decode RNAi Barcode microarray-HumanEA10506Open Biosystems
ECN4010cDNA: NEI dog cloneEA1030Open Biosystems
EDR1052Zebrafish cDNA cloneEA708Open Biosystems
EDR4009cDNA: NEI zebrafish cloneEA1030Open Biosystems
EDR4422Zebrafish EST cDNA (Exelixis)EA1030Open Biosystems
EDR5649Zebrafish ZGC PCR-Rescued cDNAEA1146Open Biosystems
EFR1003cDNA: IMAGE Fugu cDNA cloneEA708Open Biosystems
EHS1001cDNA: IMAGE Human cDNA cloneEA708Open Biosystems
EHS1015cDNA: IMAGE Human Glioma-related collectionEA65276Open Biosystems
EHS1019cDNA: Human Cystic Fibrosis cloneEA1030Open Biosystems
EHS1020cDNA: Human Cystic Fibrosis collectionEAInquireOpen Biosystems
EHS1031cDNA: Human Eye cDNA cloneEA1030Open Biosystems
EHS1193cDNA: NEI cDNA cloneEA1030Open Biosystems
EHS1194cDNA: UI Human Placenta CloneEA1030Open Biosystems
EHS4352IMAGE Plate (all species)EAInquireOpen Biosystems
EHS4415Human GPCR Putative FL cDNA Plate (IRBI)EAInquireOpen Biosystems
EMM1002cDNA: IMAGE Mouse cDNA cloneEA708Open Biosystems
EMM1005cDNA: IMAGE Mouse Lymphochip cloneEA708Open Biosystems
EMM1006cDNA: IMAGE Mouse Lymphochip collectionEAInquireOpen Biosystems
EMM1018cDNA: BMAP Non-redundant collectionEAInquireOpen Biosystems
EMM1030cDNA: IMAGE Mouse Pancreas collectionEA79889Open Biosystems
EMM1032cDNA: BMAP Non-redundant cloneEA1030Open Biosystems
EMM1147cDNA: Mouse Retina CollectionEA34891Open Biosystems
EMM1212Organ of Corti CloneEA1030Open Biosystems
EMM1213Organ of Corti CollectionEAInquireOpen Biosystems
EMM1384Mouse Eye cDNA cloneEA1030Open Biosystems
EMM3836NIA Mouse EST CloneEA1030Open Biosystems
EMM3837NIA Mouse 15K EST CollectionEAInquireOpen Biosystems
EMM4783Mouse PCR-rescued cDNA Plate (384-well)EAInquireOpen Biosystems
EOC4011cDNA: NEI rabbit cloneEA1030Open Biosystems
EPL1218IMAGE EST cDNA Plate (all species)EAInquireOpen Biosystems
EPL4612MGC Putative FL cDNA Plate (all species)EAInquireOpen Biosystems
ERM1145IMAGE Rhesus Monkey cDNAEA708Open Biosystems
ERN1016cDNA: Rat Non-redundant collectionEAInquireOpen Biosystems
ERN1017cDNA: Rat Non-redundant cloneEA1030Open Biosystems
ERN1146IMAGE Rat cDNAEA708Open Biosystems
ERN1184cDNA: University of Iowa Rat EST cloneEA708Open Biosystems
ETR4620Express-In transfection reagent 0.5ml0.5 ML1481Open Biosystems
ETR4621Express-In transfection reagent 1ml (2×0.5ml)2 x .05 mL1738Open Biosystems
ETR4622Express-In transfection reagent 5ml (5x1ml)5 x 1 mL7210Open Biosystems
ETR4623Express-In transfection reagent 10ml (10x1ml)10 x 1 mL10107Open Biosystems
EUI1044NEI non-redundant clone.EA1030Open Biosystems
EXL1051IMAGE Xenopus cDNA CloneEA708Open Biosystems
EXT1168IMAGE Xenopus tropicalis cDNAEA708Open Biosystems
HCL4517HEK293T cell lineEA3514Open Biosystems
HMR5872Human lentiviral microRNA shMIMIC Non-Silencing controlEA8190Open Biosystems
HMR5881Human lentiviral microRNA shMIMIC Empty vector – viral particles (kit of 2x25ul)EA8190Open Biosystems
ICN1378Incyte Dog cDNA CloneEA1288Open Biosystems
ICN1379Dog GEM1 Incyte CollectionEAInquireOpen Biosystems
IHS1380Incyte Full Length Human cDNA CloneEA1288Open Biosystems
IHS1382Incyte Easy-to-Spot Human cDNA CloneEA1288Open Biosystems
IHS4256Human Novel Full-Length cDNA CollectionEAInquireOpen Biosystems
IPS5428viPS-EF1a-TurboGFPEA3969Open Biosystems
IPS5445viPS Kit Mouse FeedersEA5040Open Biosystems
IPS5449viPS Vector Kit – 6 pluripotency factors plus controlEA44100Open Biosystems
IRM1377Incyte Macaque cDNA CloneEA1288Open Biosystems
IRN1383Incyte Rat cDNA CloneEA1288Open Biosystems
YSC1021Yeast: Yeast Knock Out StrainEA708Open Biosystems
YSC1048Yeast Parental Strain – BY4741EA708Open Biosystems
YSC1049Yeast Parental Strain – BY4742EA708Open Biosystems
YSC1050Yeast Parental Strain – BY4743EA708Open Biosystems
YSC1053Yeast MATa CollectionEA38561Open Biosystems
YSC1054Yeast MATalpha CollectionEA38561Open Biosystems
YSC1055Yeast Heterozygous CollectionEA48668Open Biosystems
YSC1056Yeast Homozygous Diploid CollectionEA40556Open Biosystems
YSC1057Yeast Essential CollectionEA8884Open Biosystems
YSC1058Yeast W303 MATa StrainEA708Open Biosystems
YSC1059Yeast W303 MATalpha StrainEA708Open Biosystems
YSC1060S288CEA708Open Biosystems
YSC1061Yeast Parental Strain BY4730EA708Open Biosystems
YSC1062Yeast Parental Strain BY4739EA708Open Biosystems
YSC1068Yeast HA Tag CollectionEA18476Open Biosystems
YSC1070Yeast HA Tag Individual StrainEA708Open Biosystems
YSC1071Yeast W303 Heterozygous Diploid StrainEA708Open Biosystems
YSC1073Yeast Insertional Mutant CloneEA708Open Biosystems
YSC1074Yeast Insertional Mutant CollectionEA26973Open Biosystems
YSC1177TAP Tagged ORF Collection.EAInquireOpen Biosystems
YSC1178TAP Tagged strain (endogenous promoter)EA708Open Biosystems
YSC1180Yeast Tet-promoters Hughes(yTHC)StrainEA708Open Biosystems
YSC1182Yeast Tet-promoters Hughes Collection (yTHC) KITEA14871Open Biosystems
YSC1201Yeast mTn plasmid collectionEA26973Open Biosystems
YSC1202Yeast mTn plasmidEA708Open Biosystems
YSC1210Yeast Tet-promoters Hughes Parental Strain – R1158EA708Open Biosystems
YSC1247Yeast TAP-Fusion Membrane Protein SubsetEA10686Open Biosystems
YSC1764Y800 Parental StrainEA708Open Biosystems
YSC3867Yeast ORF Clone (E. coli)EA708Open Biosystems
YSC3868Yeast ORF Collection (E. coli)EAInquireOpen Biosystems
YSC3869Yeast ORF Clone (yeast)EA708Open Biosystems
YSC3870Yeast ORF Collection (Yeast)EAInquireOpen Biosystems
YSC3880Yeast ORF Host Strain (Y258)EA708Open Biosystems
YSC4423Yeast GST-Tagged CollectionEA50728Open Biosystems
YSC4515Yeast GST-Tagged StrainEA708Open Biosystems
YSC4613Yeast Genomic Tiling Collection – GlycerolEA22982Open Biosystems
YSC5034Yeast Genomic Tiling vector – pGP564 (glycerol stock)EA2060Open Biosystems
YSC5049Yeast Cross & Capture System Collection Bait MATaEA7854Open Biosystems
YSC5050Yeast DAmp Library for 970 essential genes – diploidEA14871Open Biosystems
YSC5089Part of YSC5849  —  Yeast Protein Interactome Collection – Bait F[1 2]EA47316Open Biosystems
YSC5090Yeast DAmP Library for 842 essential genes – haploidEA12875Open Biosystems
YSC5091Part of YSC5849 —  Yeast Protein Interactome Collection – Prey F[3]EA58131Open Biosystems
YSC5092Yeast Cross & Capture System Collection Prey MATalphaEA7854Open Biosystems
YSC5093Yeast DAmP individual strain – diploidEA708Open Biosystems
YSC5094Yeast DAmP individual strain – haploidEA708Open Biosystems
YSC5095Yeast Cross & Capture strain – Bait  MATaEA708Open Biosystems
YSC5096Yeast Cross & Capture strain – Prey MATalphaEA708Open Biosystems
YSC5097Yeast Protein Interactome – Individual Bait F[1 2]EA708Open Biosystems
YSC5098Yeast Protein Interactome – Individual Prey F[3]EA708Open Biosystems
YSC5103Yeast Genomic Tiling Collection Assay Ready Pooled DNA (kit)EA3412Open Biosystems
YSC5105Non Essential Histone H3 & H4 Mutant Individual Strain (Yeast)EA708Open Biosystems
YSC5106Non Essential Histone H3 & H4 Mutant Collection (Yeast)EA7725Open Biosystems
YSC5108Yeast YFP Fusion Kinase CollectionEA3863Open Biosystems
YSC5117Yeast Barcoder Collection MATaEA13519Open Biosystems
YSC5135Histone H3 & H4 Mutant Collection (Bacteria)EA1931Open Biosystems
YSC5136Histone H3 & H4 Mutant Individual Strain (Bacteria)EA708Open Biosystems
YSC5138Essential Histone H3 & H4 Mutant Individual Strain (Yeast)EA708Open Biosystems
YSC5139Essential Histone H3 & H4 Mutant Collection (Yeast)EA1931Open Biosystems
YSC5432Molecular Barcoded Yeast (MoBY) ORF CollectionEA47316Open Biosystems
YSC5470Yeast Histone Mutant Rescue Plasmid pJP11EA708Open Biosystems
YSC5849Yeast-Interactome CollectionEA63000Open Biosystems
MBC1249PirPlus DH10bF’DOT chemically competent CellEA1790Open Biosystems
MBC3995PirPlus DH10bpir116 chemically competent CellEA1790Open Biosystems
MDR1734Zebrafish full length cloneEA1146Open Biosystems
MDR1738IMCB Zebrafish CloneEA1146Open Biosystems
MDR1739IMCB Zebrafish LibraryEAInquireOpen Biosystems
MDR4775Zebrafish MGC verified FL cDNA (IRQL)EA1146Open Biosystems
MDR4778Zebrafish MGC verified FL cDNA (IRQM)EA1146Open Biosystems
MES3974OSDupDel Gene Targeting Vector – neo (DNA)EA2016Open Biosystems
MES3975OSDupDel Gene Targeting Vector – hygro (DNA)EA2016Open Biosystems
MES3976OSDupDel Gene Targeting Vector – hprt. (DNA)EA2016Open Biosystems
MES4306C57BL6 Mouse Embryonic Fibroblasts (Mitomycin-c inactivated)EAInquireOpen Biosystems
MES4486pBS31 VectorEA6759Open Biosystems
MES4487pBS31 Prime VectorEA6759Open Biosystems
MES4488pCAGGS-FlpE VectorEA6759Open Biosystems
MES4639Inducible Gene Targeting Vector SetEA7661Open Biosystems
MES4713OSDupDel Gene Targeting Vector – neo (glycerol stock)EA3090Open Biosystems
MES4714OSDupDel Gene Targeting Vector – hygro (Glycerol)EA3090Open Biosystems
MES4715OSDupDel Gene Targeting Vector – hprt. (Glycerol)EA3090Open Biosystems
MES4758Gene targeting kit for inducible expressionEAInquireOpen Biosystems
MES4759Gene targeting kit for constitutive expressionEAInquireOpen Biosystems
MES4817C57BL6 Mouse Embryonic Fibroblasts (3 vials)EAInquireOpen Biosystems
MHS1010Human MGC Verified FL cDNA (IRAT)EA1146Open Biosystems
MHS1011Human MGC Verified FL cDNA (IRAU)EA1146Open Biosystems
MHS1768Human MGC PCR-Rescued cDNAEA1146Open Biosystems
MHS4126Human MGC Plate (pCMV-SPORT6)EAInquireOpen Biosystems
MHS4147Human MGC Plate (all vectors)EAInquireOpen Biosystems
MHS4426Human MGC Verified FL cDNA (IRCM)EA1146Open Biosystems
MHS4768Human MGC verified FL cDNA (IRQL)EA1146Open Biosystems
MHS4771Human MGC verified FL cDNA (IRQM)EA1146Open Biosystems
MHS4836Human FL cDNA Set – GPCREAInquireOpen Biosystems
MHS4837Human FL cDNA (pCMV) Set – GPCREA30900Open Biosystems
MHS4863Human FL cDNA Set – KinaseEAInquireOpen Biosystems
MHS4864Human FL cDNA (pCMV) Set – KinaseEA59483Open Biosystems
MHS4865Human FL cDNA Set – Transcription FactorEAInquireOpen Biosystems
MHS4866Human FL cDNA (pCMV) Set – Transcription FactorEA64053Open Biosystems
MHS4906Human FL cDNA (pCMV) Set – Cell CycleEA61929Open Biosystems
MHS4908Human FL cDNA Set – TransporterEAInquireOpen Biosystems
MHS4909Human FL cDNA (pCMV) Set – TransporterEA75641Open Biosystems
MHS4911Human FL cDNA Set – Nuclear ReceptorEA19570Open Biosystems
MHS4912Human FL cDNA (pCMV) Set – Nuclear ReceptorEA16738Open Biosystems
MHS4923Human FL cDNA Set – Growth FactorEA56650Open Biosystems
MHS4924Human FL cDNA (pCMV) Set – Growth FactorEA21694Open Biosystems
MHS4927Human FL cDNA Set – CytokineEAInquireOpen Biosystems
MHS4928Human FL cDNA (pCMV) Set – CytokineEA23239Open Biosystems
MHS4938Human FL cDNA Set – Ion ChannelEA74804Open Biosystems
MHS5002Human FL cDNA Set – PhosphataseEA56264Open Biosystems
MHS5003Human FL cDNA Set – UbiquitinEA58774Open Biosystems
MHS5004Human FL cDNA (pCMV) Set – PhosphataseEA23368Open Biosystems
MHS5005Human FL cDNA (pCMV) Set – UbiquitinEA23214Open Biosystems
MHS5007Human FL cDNA Set – Plasma MembraneEAInquireOpen Biosystems
MHS5008Human FL cDNA (pCMV) Set – Plasma MembraneEAInquireOpen Biosystems
MHS5010Human FL cDNA Set – Insulin Receptor SignalingEA19956Open Biosystems
MHS5024Human FL cDNA (pCMV) Set – Ion ChannelEA25106Open Biosystems
MHS5025Human FL cDNA Set – PeptidaseEAInquireOpen Biosystems
MHS5026Human FL cDNA (pCMV) Set – PeptidaseEA42230Open Biosystems
MHS5027Human FL cDNA Set – Protein KinaseEAInquireOpen Biosystems
MHS5028Human FL cDNA (pCMV) Set – Protein KinaseEA43775Open Biosystems
MHS5843MGC (all Clone including IRAT  IRAU  IRCB  IRCM  IRQL and IRQM)EAInquireOpen Biosystems
MHS5844MGC (expression-ready Clone from IRAT  IRAU  IRCB  IRCM  IRQL and IRQM)EAInquireOpen Biosystems
MMM1013Mouse MGC Verified FL cDNA (IRAV+IRAW)EA1146Open Biosystems
MMM4127Mouse MGC Plate (pCMV-SPORT6)EAInquireOpen Biosystems
MMM4769Mouse MGC verified FL cDNA (IRQL)EA1146Open Biosystems
MMM4772Mouse MGC verified FL cDNA (IRQM)EA1146Open Biosystems
MMM5083Mouse FL cDNA Set – GPCRsEAInquireOpen Biosystems
MMM5084Mouse FL cDNA (pCMV) Set – GPCRsEA28325Open Biosystems
MMM5172Mouse FL cDNA (pCMV) Set – KinaseEAInquireOpen Biosystems
MMM5173Mouse FL cDNA (pCMV) Set – Transcription FactorsEA41393Open Biosystems
MRN1768Rat MGC Verified FL cDNAEA1146Open Biosystems
MRN4591Rat MGC Verified FL cDNA PlateEAInquireOpen Biosystems
MRN4770Rat MGC verified FL cDNA (IRQL)EA1146Open Biosystems
MRN4776Rat MGC verified FL cDNA (IRQM)EA1146Open Biosystems
MRN5085Rat FL cDNA Set – GPCRsEA42488Open Biosystems
MRN5645MGC Rat collection completeEAInquireOpen Biosystems
MXL1736XGC Full Length CloneEA1146Open Biosystems
MXL4596XGC Verified FL cDNA PlateEAInquireOpen Biosystems
MXL4773Xenopus laevis MGC verified FL cDNA (IRQL)EA1146Open Biosystems
MXL4777Xenopus laevis MGC verified FL cDNA (IRQM)EA1146Open Biosystems
MXT1765cDNA: XGC Xenopus tropicalis verfied FL cloneEA1146Open Biosystems
MXT4774Xenopus tropicalis MGC verified FL cDNA (IRQL)EA1146Open Biosystems
OCE1182C. elegans ORF CloneEA1030Open Biosystems
OCE4818C. elegans Transcription Factor Clone – E. coliEA1030Open Biosystems
OCE4819C. elegans Transcription Factor Collection – E. coliEA36501Open Biosystems
OCE4820C. elegans Transcription Factor Strain – YeastEA1030Open Biosystems
OCE4821C. elegans Transcription Factor Collection – YeastEA36501Open Biosystems
ODR5134Zebrafish ORF Collaboration PlateEAInquireOpen Biosystems
ODR5433Zebrafish ORFeome Collaboration CloneEA4493Open Biosystems
OEC4626E. coli SPA-Tagged StrainEA1030Open Biosystems
OEC4627E. coli SPA-Tagged CollectionEA12167Open Biosystems
OEC4629E. coli TAP-Tagged StrainEA1030Open Biosystems
OEC4630E. coli TAP-Tagged CollectionEA4056Open Biosystems
OEC4987E. coli Keio Knockout StrainEA1030Open Biosystems
OEC4988E. coli Keio Knockout CollectionEAInquireOpen Biosystems
OEC5042E. coli Keio Knockout Parent Strain – BW25113EA708Open Biosystems
OHS1770Human ORF CloneEA3206Open Biosystems
OHS4103Human Entry ORF Set – Cell CycleEAInquireOpen Biosystems
OHS4108Human Entry ORF Set – Transcription FactorEAInquireOpen Biosystems
OHS4112Human Entry ORF Set – Oxygen BindingEA17317Open Biosystems
OHS4464Human Synthesized ORF CloneEA1030Open Biosystems
OHS4559Human ORFeome Collaboration CloneEA4493Open Biosystems
OHS4560Human ORF Collaboration PlateEAInquireOpen Biosystems
OHS4766Human synthesized ORF clone (IRCV)EA1146Open Biosystems
OHS4838Human Entry ORF Set – GPCREA40943Open Biosystems
OHS4902Mammalian SH2 Domain CollectionEA10815Open Biosystems
OHS4903Mammalian SH2 Domain CloneEA1030Open Biosystems
OHS4910Human Entry ORF Set – TransporterEAInquireOpen Biosystems
OHS4913Human Entry ORF Set – Nuclear ReceptorEA17832Open Biosystems
OHS4922Human Entry ORF Set – KinaseEAInquireOpen Biosystems
OHS4925Human Entry ORF Set – Growth FactorEA27359Open Biosystems
OHS4929Human Entry ORF Set – CytokineEA33861Open Biosystems
OHS4939Human Entry ORF Set – Ion ChannelEA34119Open Biosystems
OHS4941Human Entry ORF Set – PhosphataseEA29613Open Biosystems
OHS5016Human Entry ORF Set – Plasma MembraneEAInquireOpen Biosystems
OHS5029Human Entry ORF Set – PeptidaseEA65148Open Biosystems
OHS5030Human Entry ORF Set – Protein KinaseEA57487Open Biosystems
OHS5031Human Entry ORF Set – UbiquitinEA29741Open Biosystems
OHS5830Precision LentiORF individual clone EA5670Open Biosystems
OHS5831Precision LentiORF individual clone EA9450Open Biosystems
OHS5832Precision LentiORF RFP controlEA5040Open Biosystems
OHS5833Precision LentiORF individual plateEA4120Open Biosystems
OHS5834Precision LentiORF Expression transfection starter kitEA15120Open Biosystems
OHS5835Precision LentiORF Expression transduction starter kitEA22680Open Biosystems
OHS5836Precision LentiORF Expression viral particle starter kitEA17010Open Biosystems
OHS5893Human ORFeome Collaboration Clone (with native stop codon) – glycerolEA5040Open Biosystems
OHS5894Human ORFeome Collaboration Clone (without native stop codon) – glycerolEA5040Open Biosystems
OHS5897Precision LentiORF individual clone (with native stop codon) – glycerolEA5670Open Biosystems
OHS5898Precision LentiORF individual clone (without native stop codon) – glycerolEA5670Open Biosystems
OHS5899Precision LentiORF individual clone (with native stop codon) – viral particlesEA9450Open Biosystems
OHS5900Precision LentiORF individual clone (without native stop codon) – viral particlesEA9450Open Biosystems
OMM4760Mouse ORFeome Collaboration CloneEA4493Open Biosystems
OMM4761Mouse ORF Collaboration PlateEAInquireOpen Biosystems
OMM4767Mouse synthesized ORF clone (IRCW)EA1146Open Biosystems
OMM5895Mouse ORFeome Collaboration Clone (with native stop codon) – glycerolEA5040Open Biosystems
OMM5896Mouse ORFeome Collaboration Clone (without native stop codon) – glycerolEA5040Open Biosystems
PCE1181C. elegans Promoters LibraryEAInquireOpen Biosystems
PCE1182C. elegans Promoters CloneEA1030Open Biosystems
PEC3876E. coli Promoter CloneEA1030Open Biosystems
PEC3877E. coli Promoter CollectionEA28389Open Biosystems
PRM5038Decode RNAi-GIPZ: shRNA amplification and sequencing primer kit-200 reactionsEA1970Open Biosystems
PRM5040Decode RNAi-GIPZ: shRNA amplification and sequencing primer kit-600 reactionsEA3283Open Biosystems
PRM5340Decode RNAi-GIPZ: Forward primer for barcode amplificationEA1313Open Biosystems
PRM5341Decode RNAi-GIPZ: Reverse primer for barcode amplificationEA1313Open Biosystems
RCE1181C. elegans RNAi Collection V1.1EAInquireOpen Biosystems
RCE1182C. elegans RNAi V1.1 CloneEA1392Open Biosystems
RHS3979TRC human shRNA individual cloneEA2639Open Biosystems
RHS4080pLKO.1 empty vector controlEA4056Open Biosystems
RHS4287RNAintro GIPZ lentiviral shRNAmir transfection starter kitEA11622Open Biosystems
RHS4332Hs TRC druggable genes shRNA focused sub-library-GlycerolEAInquireOpen Biosystems
RHS4335b-gal transfection efficiency control-Glycerol stockEA4177Open Biosystems
RHS4346Non-silencing-GIPZ lentiviral shRNAmir controlEA5516Open Biosystems
RHS4351pGIPZ lentiviral empty vectorEA7289Open Biosystems
RHS4371GAPDH-GIPZ lentiviral shRNAmir positive controlEA5516Open Biosystems
RHS4372GAPDH-GIPZ lentiviral shRNAmir positive controlEA4137Open Biosystems
RHS4430Human GIPZ lentiviral shRNAmir individual cloneEA2692Open Biosystems
RHS4432Human GIPZ lentiviral shRNAmir library-Release 6.1EAInquireOpen Biosystems
RHS4434Human GIPZ lentiviral shRNAmir library-Release 6.2EAInquireOpen Biosystems
RHS4435Human GIPZ lentiviral shRNAmir library-Release 6.3EAInquireOpen Biosystems
RHS4436Human GIPZ lentiviral shRNAmir library-Release 6.4EAInquireOpen Biosystems
RHS4437Human GIPZ lentiviral shRNAmir library-Release 6.5EAInquireOpen Biosystems
RHS4438Human GIPZ lentiviral shRNAmir library-Release 6.6EAInquireOpen Biosystems
RHS4439Human GIPZ lentiviral shRNAmir library-Release 6.7EAInquireOpen Biosystems
RHS4440Human GIPZ lentiviral shRNAmir library-Release 6.8EAInquireOpen Biosystems
RHS4441Human GIPZ lentiviral shRNAmir library-Release 6.9EAInquireOpen Biosystems
RHS4442Human GIPZ lentiviral shRNAmir library-Release 6.10EAInquireOpen Biosystems
RHS4443Human GIPZ lentiviral shRNAmir library-Release 6.11EAInquireOpen Biosystems
RHS4444Human GIPZ lentiviral shRNAmir library-Release 6.12EAInquireOpen Biosystems
RHS4445Human GIPZ lentiviral shRNAmir library-Release 6.13EAInquireOpen Biosystems
RHS4459TRC Lentiviral eGFP shRNA positive control- Glycerol StockEA5408Open Biosystems
RHS4480EG5-GIPZ lentiviral shRNAmir positive controlEA5516Open Biosystems
RHS4531Human GIPZ lentiviral shRNAmir target gene setEA5516Open Biosystems
RHS4533Human pLKO.1 lentiviral shRNA target gene setEA4137Open Biosystems
RHS4584EG5-GIPZ lentiviral shRNAmir positive controlEA4137Open Biosystems
RHS4641Human GIPZ lentiviral shRNAmir library-Release 6.14EAInquireOpen Biosystems
RHS4696Human TRIPZ lentiviral inducible shRNAmir individual cloneEA2692Open Biosystems
RHS4700Human TRIPZ lentiviral inducible shRNAmir library-Release 8.1EAInquireOpen Biosystems
RHS4701Human TRIPZ lentiviral inducible shRNAmir library-Release 8.2EAInquireOpen Biosystems
RHS4702Human TRIPZ lentiviral inducible shRNAmir library-Release 8.3EAInquireOpen Biosystems
RHS4703Human TRIPZ lentiviral inducible shRNAmir library-Release 8.4EAInquireOpen Biosystems
RHS4704Human TRIPZ inducible lentiviral shRNAmir library-Release 8.5EAInquireOpen Biosystems
RHS4705Human TRIPZ inducible lentiviral shRNAmir library-Release 8.6EAInquireOpen Biosystems
RHS4706Human TRIPZ inducible lentiviral shRNAmir library-Release 8.7EAInquireOpen Biosystems
RHS4707Human TRIPZ inducible lentiviral shRNAmir library-Release 8.8EAInquireOpen Biosystems
RHS4708Human TRIPZ inducible lentiviral shRNAmir library-Release 8.9EAInquireOpen Biosystems
RHS4709Human TRIPZ inducible lentiviral shRNAmir library-Release 8.10EAInquireOpen Biosystems
RHS4710Human TRIPZ inducible lentiviral shRNAmir library-Release 8.11EAInquireOpen Biosystems
RHS4711Human TRIPZ inducible lentiviral shRNAmir library-Release 8.12EAInquireOpen Biosystems
RHS4712Human TRIPZ inducible lentiviral shRNAmir library-Release 8.13EAInquireOpen Biosystems
RHS4740Human TRIPZ  lentiviral inducible shRNAmir target gene setEA5516Open Biosystems
RHS4741RNAintro TRIPZ inducible lentiviral shRNAmir transfection kitEA11622Open Biosystems
RHS4743Non-silencing-TRIPZ lentiviral inducible shRNAmir controlEA5516Open Biosystems
RHS4744GAPDH-TRIPZ lentiviral inducible shRNAmir positive controlEA5516Open Biosystems
RHS4750TRIPZ lentiviral empty vectorEA5516Open Biosystems
RHS4827Non-silencing-TRIPZ lentiviral inducible shRNAmir controlEA4137Open Biosystems
RHS4847Decode RNAi-GIPZ: whole genome screening library-Positive selection kitEAInquireOpen Biosystems
RHS4872Human Angiogenesis TRC lentiviral shRNA gene family-GlycerolEA71199Open Biosystems
RHS4875Human Apoptosis TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4878Human Cell Cycle TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4881Human GPCR TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4884Human Kinase Activity TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4887Human Phosphatase TRC lentiviral shRNA gene family-GlycerolEA84782Open Biosystems
RHS4890Human Transcription Factors TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4893Human UbiquitinTRC lentiviral shRNA gene family-GlycerolEA79632Open Biosystems
RHS4896Human Ubiquitin ligases TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RHS4930GAPDH-TRIPZ lentiviral inducible shRNAmir positive controlEA4137Open Biosystems
RHS4973pSMP retroviral empty vector-GlycerolEA5570Open Biosystems
RHS5086RNAintro GIPZ lentiviral shRNAmir transduction kitEA17269Open Biosystems
RHS5087RNAintro TRIPZ lentiviral shRNAmir transduction kitEA17269Open Biosystems
RHS5143Hs TRC cancer genes shRNAmir focused sub-library–GlycerolEAInquireOpen Biosystems
RHS5149MAPk-GIPZ lentiviral shRNAmir positive control- Viral particlesEA4045Open Biosystems
RHS5337Decode RNAi-GIPZ: whole genome screening library-Negative selection kitEAInquireOpen Biosystems
RHS5338Decode RNAi-GIPZ: annotated genes screening library-Positive selection kitEA65663Open Biosystems
RHS5339Decode RNAi-GIPZ: annotated genes screening library-Negative selection kitEA86675Open Biosystems
RHS5478Human GIPZ lentiviral shRNAmir library-Release 6.16EAInquireOpen Biosystems
RHS5479Human GIPZ lentiviral shRNAmir library-Release 6.17EAInquireOpen Biosystems
RHS5480Human GIPZ lentiviral shRNAmir library-Release 6.18EAInquireOpen Biosystems
RHS5481Human GIPZ lentiviral shRNAmir library-Release 6.19EAInquireOpen Biosystems
RHS5482Human GIPZ lentiviral shRNAmir library-Release 6.20EAInquireOpen Biosystems
RHS5483Human GIPZ lentiviral shRNAmir library-Release 6.21EAInquireOpen Biosystems
RHS5484Human GIPZ lentiviral shRNAmir library-Release 6.22EAInquireOpen Biosystems
RHS5485Human GIPZ lentiviral shRNAmir library-Release 6.23EAInquireOpen Biosystems
RHS5486Human GIPZ lentiviral shRNAmir library-Release 6.24EAInquireOpen Biosystems
RHS5487Human GIPZ lentiviral shRNAmir library-Release 6.25EAInquireOpen Biosystems
RHS5488Human GIPZ lentiviral shRNAmir library-Release 6.26EAInquireOpen Biosystems
RHS5489Human GIPZ lentiviral shRNAmir library-Release 6.27EAInquireOpen Biosystems
RHS5850Human GIPZ lentiviral shRNAmir library-Release 6.28EAInquireOpen Biosystems
RHS5851Human GIPZ lentiviral shRNAmir library-Release 6.29EAInquireOpen Biosystems
RHS5852Human GIPZ lentiviral shRNAmir library-Release 6.30EAInquireOpen Biosystems
RHS5853Human GIPZ lentiviral shRNAmir library-Release 6.31EAInquireOpen Biosystems
RHS5906Human GIPZ lentiviral shRNAmir library-Release 6.32EA183217Open Biosystems
RHS5907Human GIPZ lentiviral shRNAmir library-Release 6.33EA183217Open Biosystems
RMM3981TRC mouse shRNA individual cloneEA2639Open Biosystems
RMM4013TRC mouse genome-wide shRNA collectionEAInquireOpen Biosystems
RMM4431Mouse GIPZ lentiviral shRNAmir individual cloneEA2692Open Biosystems
RMM4446Mouse GIPZ lentiviral shRNAmir library-Release 7.1EAInquireOpen Biosystems
RMM4447Mouse GIPZ lentiviral shRNAmir library-Release 7.2EAInquireOpen Biosystems
RMM4448Mouse GIPZ lentiviral shRNAmir library-Release 7.3EAInquireOpen Biosystems
RMM4449Mouse GIPZ lentiviral shRNAmir library-Release 7.4EAInquireOpen Biosystems
RMM4450Mouse GIPZ lentiviral shRNAmir library-Release 7.5EAInquireOpen Biosystems
RMM4451Mouse GIPZ lentiviral shRNAmir library-Release 7.6EAInquireOpen Biosystems
RMM4452Mouse GIPZ lentiviral shRNAmir library-Release 7.7EAInquireOpen Biosystems
RMM4453Mouse GIPZ lentiviral shRNAmir library-Release 7.8EAInquireOpen Biosystems
RMM4454Mouse GIPZ lentiviral shRNAmir library-Release 7.9EAInquireOpen Biosystems
RMM4455Mouse GIPZ lentiviral shRNAmir library-Release 7.10EAInquireOpen Biosystems
RMM4456Mouse GIPZ lentiviral shRNAmir library-Release 7.11EAInquireOpen Biosystems
RMM4457Mouse GIPZ lentiviral shRNAmir library-Release 7.12EAInquireOpen Biosystems
RMM4458Mouse GIPZ lentiviral shRNAmir library-Release 7.13EAInquireOpen Biosystems
RMM4532Mouse GIPZ lentiviral target gene shRNAmir setEA5516Open Biosystems
RMM4534Mouse pLKO.1 lentiviral target gene shRNA setEA4137Open Biosystems
RMM4558Mm TRC druggable genes shRNAmir focused sub-library-GlycerolEAInquireOpen Biosystems
RMM4756Mouse GIPZ lentiviral shRNAmir target gene setEA8273Open Biosystems
RMM4953Mouse Angiogenesis TRC lentiviral shRNA gene family-GlycerolEA69461Open Biosystems
RMM4954Mouse Apoptosis TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RMM4955Mouse Cell Cycle TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RMM4956Mouse GPCR TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RMM4957Mouse Kinase Activity TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RMM4958Mouse Phosphatase TRC lentiviral shRNA gene family-GlycerolEA72164Open Biosystems
RMM4959Mouse Transcription Factors TRC lentiviral shRNA gene family-GlycerolEAInquireOpen Biosystems
RMM4960Mouse UbiquitinTRC lentiviral shRNA gene family-GlycerolEA64053Open Biosystems
RMM4961Mouse Ubiquitin ligases TRC lentiviral shRNA gene family-GlycerolEA38741Open Biosystems
RMM5146Mm TRC cancer genes shRNAmir focused sub-library–GlycerolEAInquireOpen Biosystems
RMM5490Mouse GIPZ lentiviral shRNAmir library-Release 7.14EAInquireOpen Biosystems
RMM5491Mouse GIPZ lentiviral shRNAmir library-Release 7.15EAInquireOpen Biosystems
RMM5492Mouse GIPZ lentiviral shRNAmir library-Release 7.16EAInquireOpen Biosystems
RMM5493Mouse GIPZ lentiviral shRNAmir library-Release 7.17EAInquireOpen Biosystems
RMM5494Mouse GIPZ lentiviral shRNAmir library-Release 7.18EAInquireOpen Biosystems
RMM5495Mouse GIPZ lentiviral shRNAmir library-Release 7.19EAInquireOpen Biosystems
RMM5496Mouse GIPZ lentiviral shRNAmir library-Release 7.20EAInquireOpen Biosystems
RMM5497Mouse GIPZ lentiviral shRNAmir library-Release 7.21EAInquireOpen Biosystems
RMM5498Mouse GIPZ lentiviral shRNAmir library-Release 7.22EAInquireOpen Biosystems
RMM5499Mouse GIPZ lentiviral shRNAmir library-Release 7.23EAInquireOpen Biosystems
RMM5500Mouse GIPZ lentiviral shRNAmir library-Release 7.24EAInquireOpen Biosystems
RMM5501Mouse GIPZ lentiviral shRNAmir library-Release 7.25EAInquireOpen Biosystems
TLP4614Trans-Lentiviral pGIPZ Packaging System – 10rxnEA11688Open Biosystems
TLP4615Trans-Lentiviral pGIPZ Packaging System with HEK293T Cell – 10 rxnEA13395Open Biosystems
TLP4616Trans-Lentiviral pLEX Packaging System – 10 rxnEA11688Open Biosystems
TLP4617Trans-Lentiviral pLEX Packaging System with HEK293T Cell – 10 rxnEA15956Open Biosystems
TLP4691Trans-Lentiviral pGIPZ Bulk Packaging – 50 rxnEA25832Open Biosystems
TLP4692Trans-Lentiviral pGIPZ Bulk Packaging – 100 rxnEA38964Open Biosystems
TLP4693Trans-Lentiviral pLEX Bulk Packaging 50 rxnEA25832Open Biosystems
TLP4694Trans-Lentiviral pLEX Bulk Packaging 100 rxnEA38964Open Biosystems
TLP4825Trans-Lentiviral pTRIPZ Packaging SystemEA14249Open Biosystems
TLP4858Trans-Lentiviral pTRIPZ Packaging System with HEK293T CellEA15956Open Biosystems
TLP4859Trans-Lentiviral pTRIPZ Bulk Packaging – 50 rxnEA25832Open Biosystems
TLP4860Trans-Lentiviral pTRIPZ Bulk Packaging – 100 rxnEA38964Open Biosystems
VGH5518Human GIPZ lentiviral shRNAmir individual cloneEA9849Open Biosystems
VGH5526GIPZ lentiviral shRNAmir target gene set of 3EA13067Open Biosystems
VGM5520Mouse GIPZ lentiviral shRNAmir individual cloneEA9849Open Biosystems
VSH5841Human lentiviral microRNA shMIMIC – viral particles (2x25ul)EA9677Open Biosystems
 

PEI Transfection

PEI Transfection

 

 

Transfection:

 

  1. Split 293T cells one day before transfection in DMEM/10% FBS medium:
    1. 6 well dish: 0.5×106 cells
    2. 10cm dish: 4.0×106 cells
    3. 15cm dish: 9.0×106 cells

 

  1. Prior to transfection bring all reagents to room temperature.

 

  1. In a sterile tube dilute total plasmid DNA (ug) in serum-free DMEM w/o phenol red (volume of media is 10% of final volume in culture vessel). Use transgene: viral packaging (psPAX2):viral envelope (pMD2G) constructs at 4:2:1 DNA ratio

 

  1. 6 well dish: 200ul + 3 ug of total DNA
  2. 10cm dish: 1mL + 7-8 ug of total DNA
  3. 15cm dish: 2mL +  11-12 ug of total DNA

 

  1. Add PEI (1ug/uL) to the diluted DNA. Mix immediay by vortexing or pipeting. The volume of PEI used is based on a 3:1 ratio of PEI (ug):total DNA (ug).

 

  1. 6 well dish: 9ul of PEI(1ug/ul) = 9ug
  2. 10cm dish: 21ul of PEI (1ug/ul) = 21ug
  3. 15cm dish: 33ul of PEI(1ug/ul) = 33ug

 

  1. Incubate 15 minutes at RT
  2. Add DNA/PEI mixture to cells

 

  1. Harvest transfected cells and/or viral supernatant at 48 hours post-transfection

 

 

Reagents:

 

PEI (1ug/ul) – PEI is Polyethylenimine 25kD linear from Polysciences (cat# 23966-2). To make a stock solution:

  • Dissolve PEI in endotoxin-free dH2O that has been heated to ~80°C.
  • Let cool to room temperature.
  • Neutralize to pH 7.0, filter sterilize (0.22um), aliquot and store at -20°C; a working stock can be kept at 4°C.
  • 货号 品名 价格¥ 规格 品牌 备注
    23966-2 POLYETHYLENIMINE LINEAR 2864 2 g polysciences 大量现货
    24765-2 POLYETHYLENEIMINE 'MAX' 3184 2 g polysciences 转染效率高30% 更好溶解
    78002qf01 线性PEI转染液 200 1ml jinpanbio 23966配制
    78002qf02 线性PEI转染液 600 50ml jinpanbio 23966配制
    78002qf03 线性PEI转染液 1800 500ml jinpanbio 23966配制
    78003qf01 线性PEI转染液 200 1ml jinpanbio 24765配制
    78003qf02 线性PEI转染液 700 50ml jinpanbio 24765配制
    78003qf03 线性PEI转染液 2200 500ml jinpanbio 24765配制
    78004qf01 POLYETHYLENIMINE LINEAR 1432 1g jinpanbio 23966分装
    78005qf01 POLYETHYLENEIMINE 'MAX'(40 000M.W.* 1592 1g jinpanbio 24765分装

Polyplus Transfection 代理


Polyplus Transfection 代理

简要描述:Polyplus Transfection代理,Polyplus Transfection中国代理,Polyplus Transfection上海代理,Polyplus Transfection代理,Polyplus Transfection
Polyplus Transfection专业代理-上海金畔生物科技有限公司,具体产品信息欢迎电询:021-50837765

详细介绍

产品咨询

世界*实验材料供应商Polyplus Transfection正式上海金畔为其中国代理,Polyplus Transfection在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔一直秉承为中国科研客户带来的产品,的服务, Polyplus Transfection就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Polyplus Transfection中国代理,Polyplus Transfection上海代理,Polyplus Transfection北京代理,Polyplus Transfection广东代理,Polyplus Transfection江苏代理 Polyplus Transfection湖北代理, Polyplus Transfection天津, Polyplus Transfection黑龙江代理, Polyplus Transfection内蒙古代理, Polyplus Transfection吉林代理, Polyplus Transfection福建代理, Polyplus Transfection江苏代理, Polyplus Transfection浙江代理, Polyplus Transfection四川代理,

 

公司:www.polyplus-transfection.com

PolyPlus-transfection(PT)是一家位于法国的生物技术公司,目前已经通过了ISO9001:2000质量体系认证。该公司拥有十几年的的转染试剂研发经验,已经开发了一系列的转染试剂,如:基因的体内和体外转染试剂、寡核苷酸转染试剂和siRNA转染试剂等,可以根据不同的需要获得稳定或瞬时的转染效果。
JetPEITM转染试剂
该试剂是阳离子化的大分子多聚乙烯亚胺(PEI)及其衍生物,其与外源基因结合形成颗粒状,与细胞表面的蛋白多糖结合,通过细胞”内吞作用”进入细胞。PEI-复合体具有质子海绵的特性,能吸收溶酶体的H+,在复合体中形成酸性环境使核酸酶失活,保护DNA免受核酸酶的降解。进入细胞后,JetPEI复合体囊泡肿胀破裂,将DNA释放到细胞质中。
特点:
l 转染效率高,重复性好
l 可转染许多种细胞系,对粘附细胞和悬浮细胞同样有效
l 对原代细胞也具有很好的转染结果
l 也可以转染寡核苷酸
l 培养基可含或不含血清
l 极低的细胞毒性
l 方法简便,省时省力
基本步骤
1) JetPEI溶液2μl和质粒DNA 1μg混合5-30分钟。
2) 将JetPEI-DNA复合体溶液加入到细胞液中(可以在含血清的培养基中)。
3) 检测
N/P比率(影响转染效率的关键)
N/P比率是对JetPEI复合体的所带电荷的度量。它指的是DNA分子带负电的磷酸基团与JetPEI中N残基的摩尔比,是衡量核酸与转染试剂比例的参数。从理论上讲,JetPEI/DNA复合体的电中性为N/P=2-3,但实际操作时,N/P=5-10效果较好。
转染试剂的类型
1.JetPEI转染试剂
JetPEI是PolyPlus-transfection的主导产品,毒性低,对细胞不会造成伤害。它能把基因转入各种粘附或悬浮的细胞系中以及原代细胞。
2.In vivo-JetPEI转染试剂
通过静脉、大脑、腹膜以及气管等体内途径进行转染,目前已在小鼠、大鼠、鸭、豚鼠和猴等动物身上取得成功。
3.Tagged JetPEIs:细胞介导的转染试剂
Tagged JetPEIs是偶联有配体的PEI衍生物,可将基因转入特殊的细胞。
1) JetPEI-Gal & in vivo-JetPEI-Gal(肝细胞介导)
PEI衍生物,偶联有半乳糖。受体细胞:细胞表面表达半乳糖特异性膜血凝素的细胞,如肝细胞。
2) JetPEI-Man & in vivo-JetPEI-Man(树突状细胞和巨噬细胞)
PEI衍生物,偶联有甘露糖。受体细胞:细胞表面表达甘露糖特异性受体的细胞,如巨噬细胞和树突状细胞。
3) JetPEI-RGD(上皮细胞和内皮细胞)
PEI衍生物,偶联有RGD肽链。受体细胞:细胞表面表达整合素的细胞,如上皮细胞和内皮细胞。 4.Fluo JetPEI:荧光转染试剂
1) JetPEI- FluoF
PEI衍生物,偶联荧光素FluoF(激发波长490nm,发射波长520nm),通常用于胞内定位研究,适于荧光显微镜和流式细胞仪的检测。
2) JetPEI- FluoR
PEI衍生物,偶联四甲基罗丹明FluoR(激发波长555nm,发射波长580nm),常用于双色标记和协同定位的实验中,如用于绿色荧光蛋白或FITC标记的质粒的转染。
3) In vivo-JetPEI-FluoF & In vivo-JetPEI-FluoR
PolyPlus-transfection也提供体内转染的荧光试剂,常用于生物分布研究。
5.JetPEI-Biotin和in vivo-JetPEI-Biotin
PEI衍生物,偶联Biotin,通过链亲和素检测JetPEI/DNA在细胞内的定位和分布。

 

 

上海金畔生物科技有限公司

Polyplus Transfection代理


Polyplus Transfection代理

简要描述:Polyplus Transfection代理,Polyplus Transfection中国代理,Polyplus Transfection上海代理,Polyplus Transfection代理,Polyplus Transfection
Polyplus Transfection专业代理-上海金畔生物科技有限公司,具体产品信息欢迎电询

详细介绍

产品咨询

世界*实验材料供应商BioLegend正式上海金畔为其中国代理,BioLegend在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔一直秉承为中国科研客户带来的产品,的服务, BioLegend就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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